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1. 发明专利

JP2011200120A Method for evaluating soil health by activity measurement of pectin-degrading enzyme 审中-公开
标题翻译：通过PECTIN降解酶活性测定评估土壤健康的方法
公开(公告)号：JP2011200120A
公开(公告)日：2011-10-13
申请号：JP2010067640
申请日：2010-03-24
申请人： Kansai Electric Power Co Inc:The , Kobe Univ , Matsumoto Biseibutsu Kenkyusho:Kk , 国立大学法人神戸大学 , 株式会社松本微生物研究所 , 関西電力株式会社
发明人： TAWARADA KEI , MIYASAKA HITOSHI , AE NORIJI , NAKAMURA SHINTARO , MAKI TAKAAKI , SARUTA TOSHIYASU
IPC分类号： C12Q1/06 , C12N1/14
摘要： PROBLEM TO BE SOLVED: To provide a method for evaluating soil health which obtains the result in a short time while considering antagonism to other microorganisms in soil.SOLUTION: The method for evaluating soil health by activity measurement of pectin-degrading enzyme includes (i) adding a pectin to a sample of soil of evaluated object, culturing the sample, and measuring a pectin-degrading enzyme activity after the culture, (ii) culturing the sample without adding a pectin to a sample of soil of evaluated object and measuring a pectin-degrading enzyme activity after the culture, (iii) obtaining a difference between the pectin degrading enzyme activity measured by (i) and the pectin-degrading enzyme activity measured by (ii), and making the difference as a pectin-degrading enzyme induction activity of the soil of evaluated object, and (iv) comparing the pectin-degrading enzyme induction activity of the soil of evaluated object with a previously calculated pectin-degrading enzyme induction activity of sound soil and determining the soil of evaluated object as unsound soil when the pectin-degrading enzyme induction activity of the soil of evaluated object is higher than the pectin-degrading enzyme induction activity of the sound soil by a fixed ratio.
摘要翻译：要解决的问题：提供一种评估土壤健康的方法，在考虑土壤中其他微生物的拮抗作用的同时，在短时间内获得结果。解决方案：通过果胶降解酶的活性测定来评估土壤健康的方法包括（i）在评价对象的土壤样品中加入果胶，培养样品，培养后测定果胶降解酶活性，（ii）培养不添加果胶的样品至测定对象的土壤样品，测定果胶（iii）获得通过（i）测定的果胶降解酶活性与通过（ii）测定的果胶降解酶活性之间的差异，并且使差异作为果胶降解酶诱导活性的评估对象的土壤，以及（iv）比较评估对象的土壤的果胶降解酶诱导活性与先前计算的果胶降解酶诱导当评估对象土壤的果胶降解酶诱导活性高于固体比例下的土壤果胶降解酶诱导活性时，声土的活性和评价对象的土壤测定为不健康的土壤。

2. 发明专利

JP2012080829A Greening material and method for producing the same 审中-公开
标题翻译：绿色材料及其制造方法
公开(公告)号：JP2012080829A
公开(公告)日：2012-04-26
申请号：JP2010230038
申请日：2010-10-12
申请人： Kansai Electric Power Co Inc:The , Matsumoto Biseibutsu Kenkyusho:Kk , 株式会社松本微生物研究所 , 関西電力株式会社
发明人： OKUDA EIJI , SAKAKURA YUKI , MAKI TAKAAKI , SARUTA TOSHIYASU , KURISU TOSHIHIRO
IPC分类号： A01G7/00 , A01G1/00
摘要： PROBLEM TO BE SOLVED: To provide a greening material capable of holding ectomycorrhizal fungi or arbuscular mycorrhizal fungi at high density and sufficiently exhibiting the effect of the microorganisms when the material is applied to the soil.SOLUTION: The pH of vegetable carbide is adjusted to 5.0-8.0 by adding at least one of organic acid, vegetable juice and fruit juice to the vegetable carbide, and ectomycorrhizal fungi and/or arbuscular mycorrhizal fungi are held to the vegetable carbide to obtain the greening material. As a result, the vegetable carbide, whose chemical characteristic is normally mildly alkaline or alkaline, can be optimized as a material for holding ectomycorrhizal fungi and arbuscular mycorrhizal fungi which like mildly acidic or acidic conditions. With this greening material, ectomycorrhizal fungi and/or arbuscular mycorrhizal fungi can be efficiently propagated on the vegetable carbide and held at high density, and the effect of the microorganisms can be stably exhibited when the greening material is applied to the soil.
摘要翻译：要解决的问题：提供能够以高密度保持外生菌根真菌或丛枝菌根真菌的绿化材料，并且当将材料施用于土壤时充分显示微生物的作用。解决方案：将植物碳酸钙的pH值调节至5.0-8.0，将有机酸，蔬菜汁和果汁中的至少一种加入到植物碳化物中，将外生菌根真菌和/或丛枝菌根真菌保存在植物碳化物获得绿化材料。结果，其化学特性通常为碱性或碱性的植物碳化物可以优化为用于保持外来菌根真菌和类似轻度酸性或酸性条件的菌根真菌的物质。利用这种绿色材料，外生菌根真菌和/或丛枝菌根真菌可以在植物碳化物上高效繁殖并保持高密度，并且当将绿化材料施用于土壤时，可以稳定地显示微生物的效果。版权所有（C）2012，JPO＆INPIT

3. 发明专利

JP2015047130A 緑化資材及びその製造方法有权
标题翻译：绿色材料及其生产方法
公开(公告)号：JP2015047130A
公开(公告)日：2015-03-16
申请号：JP2013181883
申请日：2013-09-03
申请人：関西電力株式会社 , Kansai Electric Power Co Inc:The , 株式会社松本微生物研究所 , Matsumoto Biseibutsu Kenkyusho:Kk
发明人： OKUDA EIJI , SAKAKURA HIROKI , MAKI TAKAAKI , SARUTA TOSHIYASU , KURISU TOSHIHIRO
IPC分类号： A01G7/00 , A01G1/00
摘要：【課題】従来の緑化資材よりも菌根菌を高密度に保持させることができ、更に土壌に使用した際もより優れた効果を安定して発揮させることが可能な緑化資材を提供する。【解決手段】植物炭化物に酸性の液体を添加することによりpH5.0〜8.0としたものに、外生菌根菌又はアーバスキュラー菌根菌であるイグチ科チチアワタケ1140−TK44菌株(NITE AP−01658)、キシメジ科シャカシメジ273−TS49菌株(NITE AP−01659)、及びグロマス(Glomus)属KANSO−19菌株のいずれか一株又は複数株を保持させることにより緑化資材を形成する。【選択図】図1
摘要翻译：要解决的问题：提供能够以比常规的更高的密度保持菌根真菌的绿化材料，并且甚至在土壤中使用时也稳定地表现出优异的效果。解决方案：通过保持任何一种或多种菌株的芽孢杆菌形成绿色材料家蚕科家族Boleaceae的菌株1140-TK44（NITE AP-01658），家蚕Tricholoma的Lyophyllum fumosum菌株273-TS49（NITE AP-01659）和作为外生菌根真菌或丛枝菌根真菌的Glomus属的KANSO-19菌株，通过加入酸性液体得到pH5.0〜8.0。

4. 发明专利

JP2007176759A Microbial material using earthworm excrement and its use 审中-公开
标题翻译：使用地球化学技术的微生物材料及其使用
公开(公告)号：JP2007176759A
公开(公告)日：2007-07-12
申请号：JP2005378545
申请日：2005-12-28
申请人： Health & Life:Kk , Matsumoto Biseibutsu Kenkyusho:Kk , 株式会社ヘルスアンドライフ , 株式会社松本微生物研究所
发明人： MAKI TAKAAKI , SASAHIRA TAKASHI , SARUTA TOSHIYASU , NAKAMURA AKINARI
IPC分类号： C05F3/00 , A01G7/00 , A01G7/06 , B09B3/00 , C02F11/02 , C05F11/00 , C05F11/08 , C09K17/32 , C09K101/00
CPC分类号： Y02A40/205 , Y02W10/27
摘要： PROBLEM TO BE SOLVED: To provide a microbial material using an earthworm excrement agglomerating soil and exhibiting a fertilization effect within a short period after the application to the soil and also which is not bulky and being easily transported and handled.
SOLUTION: The microbial material using the earthworm excrement is obtained by adding strict aerobic bacteria containing microaerobic bacteria to an extract solution of soil microorganisms contained in the earthworm excrement (hereinafter referred simply as the extract solution). The extract solution is a liquid portion obtained by extracting the soil microorganisms by adding predetermined water, for example ionized water or the like containing no chlorine or various kinds of chemical substances, to the earthworm excrement. The obligate aerobic bacteria containing the slightly aerobic bacteria which are added to the extract solution are photosynthesis bacteria, such as red non-sulfur bacteria, and microorganism containing lactic acid bacteria or the like. When the red non-sulfur bacteria are used, Rhodobacter capsulata are especially preferable while the kind of bacteria is out of the question.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供使用蚯蚓排泄物附聚土并在施用于土壤之后的短时间内施肥的微生物物质，并且其不笨重且易于运输和处理。解决方案：使用蚯蚓粪便的微生物物质是通过将含有微生物菌的严格需氧菌添加到蚯蚓粪中含有的土壤微生物的提取液中（以下简称为提取液）得到的。提取液是通过向蚯蚓粪中添加规定的水（例如，不含氯或各种化学物质的电离水等）提取土壤微生物而获得的液体部分。添加到提取液中的含有微生物需氧细菌的有义需氧细菌是光合细菌，如红色非硫细菌，含有乳酸菌的微生物等。当使用红色非硫细菌时，特别优选荚果红细菌，而细菌的种类是不合适的。版权所有（C）2007，JPO＆INPIT

5. 发明专利

JP2013173707A Plant disease controlling material and method for controlling plant disease 有权
标题翻译：植物病害控制材料和控制植物病害的方法
公开(公告)号：JP2013173707A
公开(公告)日：2013-09-05
申请号：JP2012039929
申请日：2012-02-27
申请人： Kansai Electric Power Co Inc:The , 関西電力株式会社 , General Environmental Technos Co Ltd , 株式会社環境総合テクノス
发明人： OKUDA EIJI , SAKAI KENJI , KURISU TOSHIHIRO , MAKI TAKAAKI , SARUTA TOSHIYASU
IPC分类号： A01N63/00 , A01P3/00
摘要： PROBLEM TO BE SOLVED: To provide a plant disease controlling material for a white root rot disease and a valsa canker disease.SOLUTION: A plant disease controlling material having excellent antagonistic action against a white root rot disease and a valsa canker disease is composed by supporting a Bacillus methylotrophicus KB-1 strain (NITE AP-1234), a Bacillus sp. KB-B strain (NITE AP-1235), a Bacillus amyloliquefaciens KB-7 strain (NITE AP-1236), a Streptomyces sp. KS-21 strain (NITE AP-1237), a Millerozyma sp. KC-10 strain (NITE AP-1238) and mycorrhizal fungi with a neutral-alkaline woody carbide.
摘要翻译：要解决的问题：提供白根腐病和缬草溃疡病的植物病害防治材料。解决方案：对白根腐病和valsa溃疡病具有优异的拮抗作用的植物病害防治材料由支持甲状芽孢杆菌KB-1菌株（NITE AP-1234），芽孢杆菌属 KB-B菌株（NITE AP-1235），解淀粉芽孢杆菌KB-7菌株（NITE AP-1236），链霉菌属（Streptomyces sp。 KS-21菌株（NITE AP-1237），Millerozyma sp。 KC-10菌株（NITE AP-1238）和具有中性碱性木质碳化物的菌根真菌。

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