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1. 发明专利

JP2589287B2 失效
公开(公告)号：JP2589287B2
公开(公告)日：1997-03-12
申请号：JP33826789
申请日：1989-12-28
申请人： KITSUKOOMAN KK , NODA SANGYO KAGAKU KENKYUSHO
发明人： OOTAKE HIDEKO , KOYAMA TAIJI , HORIUCHI TATSUO , NAKANO EIICHI
IPC分类号： C12N9/04 , C12N15/09 , C12N15/53 , C12R1/01 , C12R1/19 , C12R1/20

2. 发明专利

JPH0632608B2 失效
公开(公告)号：JPH0632608B2
公开(公告)日：1994-05-02
申请号：JP20129086
申请日：1986-08-29
申请人： KITSUKOOMAN KK , NODA SANGYO KAGAKU KENKYUSHO
发明人： KOYAMA TAIJI , NAKANO EIICHI , SUZUKI MASARU , YAMAMOTO HIDEKO
IPC分类号： C12N15/09 , C12N9/06 , C12N15/00 , C12R1/07 , C12R1/19 , C12N15/53

3. 发明专利

JPS62205786A NOVEL RECOMBINANT DNA 失效
公开(公告)号：JPS62205786A
公开(公告)日：1987-09-10
申请号：JP4824686
申请日：1986-03-07
申请人： KIKKOMAN CORP , NODA SANGYO KAGAKU KENKYUSHO
发明人： KOYAMA TAIJI , NAKANO EIICHI , KITAO SATORU , YAMAMOTO HIDEKO , SUZUKI MASARU , NAKAJIMA YASUHIKO
IPC分类号： C12N15/09 , C12N9/78 , C12N9/80 , C12R1/19
摘要： PURPOSE:To obtain a recombinant DNA useful for the economical and efficient production of creatinase, by inserting a DNA containing creatinase-coding gene into a vector DNA. CONSTITUTION:Bacterial cells obtained by centrifugal separation of culture product of Flavobacterium U-188 strain are digested with a restriction enzyme and purified to obtain a mixture of DNA fragments. The mixture is mixed with a vector DNA (e.g. plasmid pBR322DNA) and made to react e.g. with T4DNA ligase at 4-37 deg.C for >=1hr at an enzyme concentration of 1-100 unit to obtain a recombinant DNA. The DNA is transduced e.g. into E.coli DHI and a creatinase-producing strain is selected from the obtained strain. The selected strain is cultured and the resultant microbial cell is treated and purified to obtain a novel recombinant DNA.

4. 发明专利

JPS60118188A NOVEL RECOMBINANT DNA 失效
公开(公告)号：JPS60118188A
公开(公告)日：1985-06-25
申请号：JP22447283
申请日：1983-11-30
申请人： NODA SANGYO KAGAKU KENKYUSHO
发明人： NAKANO EIICHI , MASUDA TSUTOMU , KOYAMA TAIJI , KITAO SATORU
IPC分类号： C12N15/09 , C12N7/00 , C12N9/04 , C12N15/00 , C12N15/70 , C12R1/19
摘要： NEW MATERIAL:A recombinant DNA having if necessary an endonuclease cleavage site in a DNA part on the upstream side of a late promotor necessary for transfer of genetic information about film protein biosynthesis to the adhesive end thereof and the aimed genetic information inserted into an endonuclease cleavage site present in a DNA part on the downstream side of the late promoter of a new species bacteriophage DNA having an endonulcease cleavage site on the downstream side of the late promoter and to the adhesive end thereof. USE:For biosynthesis of protein, e.g. enzymic protein, hormone, antigen, antibody, etc. in large quantities. PREPARATION:DNA containing genetic information, e.g. homoserine dehydrogenase gene, derived from coliform bacilli, is inserted into a DNA part on the downstream side of a late promoter on the DNA of a bacteriophage and to the adhesive end thereof to give the aimed recombinant DNA.

5. 发明专利

JPS58212781A New species bacteriophage and method for breeding the same 失效
标题翻译：新物种及其育种方法
公开(公告)号：JPS58212781A
公开(公告)日：1983-12-10
申请号：JP9306282
申请日：1982-06-02
申请人： Noda Sangyo Kagaku Kenkyusho
发明人： NAKANO EIICHI , MASUDA TSUTOMU , KOYAMA TAIJI , KITAO SATORU
IPC分类号： C12N15/09 , C12N1/21 , C12N7/00 , C12N15/00 , C12N15/70 , C12N15/73 , C12R1/91
CPC分类号： C12N15/70 , C12N15/73
摘要： PURPOSE: A new species bacteriophage, having DNA parts cleaved at specific sites, and capable of effectively and remarkably facilitating the insertion of the desired genetic information into the above-mentioned cleaved sites.
CONSTITUTION: A new species bacteriophage obtained by hybridizing a λ type bacteriophage resistant to an edonuclease and/or a λ type bacteriophage having one or more sites cleaved by the endonuclease in a reconstitutive number in cleaving the bacteriophage DNA with the endonuclease, and rejoining the resultant DNA fragments with the DNA parts on the upstream side from the late promoter necessary for the transcription of the genetic information of the biosynthesis of a film protein to the sticky end thereof by a DNA ligase with a λ type bacteriophage having one or more sites cleaved by another endonuclease on the downstream side from the late promoter to the sticky end thereof.
COPYRIGHT: (C)1983,JPO&Japio
摘要翻译：目的：一种新的物种噬菌体，其具有在特定部位切割的DNA部分，并且能够有效和显着地促进将所需遗传信息插入上述切割位点。构成：通过将具有一个或多个由核酸内切酶切割的内切核酸酶切割的一个或多个位点的λ型噬菌体杂交到具有内切核酸酶的噬菌体DNA切割的重组数中，并且将所得物质重新连接而获得的新物种噬菌体 DNA片段的DNA部分位于晚期启动子上游侧，用于通过DNA连接酶转录膜蛋白的生物合成的基因信息到其粘性末端，该DNA连接酶具有一个或多个由一个或多个位点切割的位点的λ型噬菌体从后期启动子到其粘性末端的下游侧的另一种核酸内切酶。

6. 发明专利

JPH0690765A L-FUCOSE DEHYDROGENASE GENE, NEW RECOMBINANT DNA AND PRODUCTION OF L-FUCOSE DEHYDROGENASE 失效
公开(公告)号：JPH0690765A
公开(公告)日：1994-04-05
申请号：JP24337292
申请日：1992-09-11
申请人： KIKKOMAN CORP , NODA SANGYO KAGAKU KENKYUSHO
发明人： OTAKE HIDEKO , KOYAMA TAIJI , HORIUCHI TATSUO , NAKANO EIICHI
IPC分类号： C12N1/21 , C12N9/04 , C12N15/09 , C12N15/53 , C12R1/19 , C12R1/38
摘要： PURPOSE:To obtain a new gene useful in the production of L-fucose dehydrogenase useful for determination, etc., of L-fucose in serum, etc. CONSTITUTION:A L-fucose dehydrogenase gene coding an amino sequence of the formula. Phage particles are formed from a recombinant phage DNA of DNA fragment containing a gene derived from Pseudomonas sp No. 01143 and coding L-FDH and a bacteriophage vector DNA capable of incorporating Sau3AI fragment and Pseudomonas P2392, etc., is infected with these phage particles to afford a plaque of recombinant phage having various DNA fragments. Plaque hybridization is carried out using an oligonucleotide labelled with [gamma P]ATP, etc., as a probe from among this plaque and a purified phage DNA is obtained from the resultant recombinant phage and unnecessary DNA is removed to provide the objective DNA fragment containing a DNA fragment containing gene coding L-FDH.

7. 发明专利

JPH03201988A N-ACETYLMANNOSAMINE DEHYDROGENASE GENE, NEW RECOMBINANT DNA AND PRODUCTION OF N-ACETYLMANNOSAMINE DEHYDROGENASE 失效
公开(公告)号：JPH03201988A
公开(公告)日：1991-09-03
申请号：JP33826789
申请日：1989-12-28
申请人： KIKKOMAN CORP , NODA SANGYO KAGAKU KENKYUSHO
发明人： OTAKE HIDEKO , KOYAMA TAIJI , HORIUCHI TATSUO , NAKANO EIICHI
IPC分类号： C12N9/04 , C12N15/09 , C12N15/53 , C12R1/01 , C12R1/19 , C12R1/20
摘要： NEW MATERIAL:An N-acetylmannosamine dehydrogenase gene derived from a bacterium belonging to the genus Flavobacterium prescribed by a restriction endonuclease cleavage map shown by formula I (S is SalI; A is AatII; Sp is SphI). EXAMPLE:An N-acetylmannosamine dehydrogenase gene to code an amino acid sequence shown by formula II. USE:Determination of sialic acid. PREPARATION:A chromosome DNA obtained from cell of Flavobacterium sp. no. 141-8 strain is integrated with phage vector lambdagt11 and Escherichia coli Y-1,090 is infected with recombinant phage. Phage DNA is purified from recombinant phage containing an N-acetylmannosamine dehydrogenase gene fraction and labeled to give the title incomplete gene DNA, which is used as a probe and a DNA fraction containing the title complete gene is obtained from a chromosome library prepared by using phage vector EMBL3 as a vector.

8. 发明专利

JPH05115281A NEW SARCOSINE OXIDASE M, ITS GENE AND NEW RECOMBINANT DNA AND PRODUCTION OF NEW SARCOSINE OXIDASE M 失效
公开(公告)号：JPH05115281A
公开(公告)日：1993-05-14
申请号：JP28012691
申请日：1991-10-25
申请人： KIKKOMAN CORP , NODA SANGYO KAGAKU KENKYUSHO
发明人： ICHIKAWA TOSHIO , SUZUKI MASARU , KOYAMA TAIJI , NAKANO EIICHI
IPC分类号： C12N1/21 , C12N9/06 , C12N15/53 , C12Q1/26 , C12R1/185
摘要： PURPOSE:To obtain a new sarcosine oxidase M suitable as an enzyme for diagnosis of kidney diseases, etc. CONSTITUTION:Sarcosine oxidase M having an amino acid sequence of the formula. The sarcosine oxidase M is obtained by culture of a microorganism [e.g. Escherichia coli JM109 (FERM P-3604)] having sarcosine oxidase producing ability and belonging to the genus Escherichia, and has high substrate specificity and thermal stability.

9. 发明专利

JPS6359893A NOVEL RECOMBINANT DNA 失效
公开(公告)号：JPS6359893A
公开(公告)日：1988-03-15
申请号：JP20128986
申请日：1986-08-29
申请人： KIKKOMAN CORP , NODA SANGYO KAGAKU KENKYUSHO
发明人： KOYAMA TAIJI , NAKANO EIICHI , SUZUKI MASARU , YAMAMOTO HIDEKO
IPC分类号： C12N15/09 , C12N9/06 , C12N15/00 , C12R1/07 , C12R1/19
摘要： PURPOSE:To improve the productivity of a heat-resistant sarcosine oxidase N, by inserting a DNA containing a gene coding a heat-resistant sarcosine oxidase N into a vector DNA to produce a recombinant DNA. CONSTITUTION:A recombinant DNA is prepared by inserting a DNA containing a gene coding a heat-resistant sarcosine oxidase N into a vector DNA. The recombinant is included in a cell of a bacterial strain belonging to Escherichia genus and the obtained strain capable of producing heat-resistant sarcosine oxidase N is cultured in a medium. The objective heat-resistant sarcosine oxidase N can be produced in the medium in high efficiency without addition of creatinine, creatine and/or sarcosine to the medium. The vector DNA is e.g. plasmid pC18 DNA.

10. 发明专利

JPH0616712B2 失效
公开(公告)号：JPH0616712B2
公开(公告)日：1994-03-09
申请号：JP22447283
申请日：1983-11-30
申请人： NODA SANGYO KAGAKU KENKYUSHO
发明人： NAKANO EIICHI , MASUDA TSUTOMU , KOYAMA TAIJI , KITAO SATORU
IPC分类号： C12N15/09 , C12N7/00 , C12N9/04 , C12N15/00 , C12N15/70 , C12R1/19 , C12N15/73

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