基本信息:
- 专利标题： New species bacteriophage and method for breeding the same
- 专利标题（中）：新物种及其育种方法
- 申请号：JP9306282 申请日：1982-06-02
- 公开(公告)号：JPS58212781A 公开(公告)日：1983-12-10
- 发明人： NAKANO EIICHI , MASUDA TSUTOMU , KOYAMA TAIJI , KITAO SATORU
- 申请人： Noda Sangyo Kagaku Kenkyusho
- 专利权人： Noda Sangyo Kagaku Kenkyusho
- 当前专利权人： Noda Sangyo Kagaku Kenkyusho
- 优先权： JP9306282 1982-06-02
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12N1/21 ; C12N7/00 ; C12N15/00 ; C12N15/70 ; C12N15/73 ; C12R1/91
摘要：
PURPOSE: A new species bacteriophage, having DNA parts cleaved at specific sites, and capable of effectively and remarkably facilitating the insertion of the desired genetic information into the above-mentioned cleaved sites.
CONSTITUTION: A new species bacteriophage obtained by hybridizing a λ type bacteriophage resistant to an edonuclease and/or a λ type bacteriophage having one or more sites cleaved by the endonuclease in a reconstitutive number in cleaving the bacteriophage DNA with the endonuclease, and rejoining the resultant DNA fragments with the DNA parts on the upstream side from the late promoter necessary for the transcription of the genetic information of the biosynthesis of a film protein to the sticky end thereof by a DNA ligase with a λ type bacteriophage having one or more sites cleaved by another endonuclease on the downstream side from the late promoter to the sticky end thereof.
COPYRIGHT: (C)1983,JPO&Japio
摘要（中）：
CONSTITUTION: A new species bacteriophage obtained by hybridizing a λ type bacteriophage resistant to an edonuclease and/or a λ type bacteriophage having one or more sites cleaved by the endonuclease in a reconstitutive number in cleaving the bacteriophage DNA with the endonuclease, and rejoining the resultant DNA fragments with the DNA parts on the upstream side from the late promoter necessary for the transcription of the genetic information of the biosynthesis of a film protein to the sticky end thereof by a DNA ligase with a λ type bacteriophage having one or more sites cleaved by another endonuclease on the downstream side from the late promoter to the sticky end thereof.
COPYRIGHT: (C)1983,JPO&Japio
目的：一种新的物种噬菌体，其具有在特定部位切割的DNA部分，并且能够有效和显着地促进将所需遗传信息插入上述切割位点。 构成：通过将具有一个或多个由核酸内切酶切割的内切核酸酶切割的一个或多个位点的λ型噬菌体杂交到具有内切核酸酶的噬菌体DNA切割的重组数中，并且将所得物质重新连接而获得的新物种噬菌体 DNA片段的DNA部分位于晚期启动子上游侧，用于通过DNA连接酶转录膜蛋白的生物合成的基因信息到其粘性末端，该DNA连接酶具有一个或多个由一个或多个位点切割的位点的λ型噬菌体 从后期启动子到其粘性末端的下游侧的另一种核酸内切酶。
公开/授权文献:
- JPH0149479B2 公开/授权日：1989-10-24