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1. 发明专利

JPH0833482A NEW SORBITOL DEHYDROGENASE, ITS PRODUCTION, REAGENT FOR DETERMINATION OF SORBITOL USING THE ENZYME AND DETERMINATION METHOD 失效
公开(公告)号：JPH0833482A
公开(公告)日：1996-02-06
申请号：JP29670194
申请日：1994-11-30
申请人： KIKKOMAN CORP
发明人： NANBARA TOMOYUKI , SUZUKI MASARU
IPC分类号： C12N9/04 , C12Q1/32 , C12R1/38
摘要： PURPOSE:To obtain a new sorbitol dehydrogenase produced by the culture of microbial cell belonging to the genus Pseudomonas and capable of producing sorbitol dehydrogenase, extremely useful for the enzymatic assay of D-sorbitol and usable e.g. for the diagnosis of diabetes. CONSTITUTION:This new sorbitol dehydrogenase useful e.g. for the enzymatic assay of D-sorbitol is produced by culturing a microbial strain belonging to the genus Pseudomonas and capable of producing sorbitol dehydrogenase [e.g. Pseudomonas sp.KS-E1806 (FERM P-14229)] in a medium. The enzyme is effective to oxidize D-sorbitol in the presence o NAD to produce D-fructose and NADH and specifically act on D-sorbitol and galactitol, has an optimum pH of about 10 and stable pH of 5.5-10.5 (treatment at 30 deg.C for 4hr), completely deactivated at pH =11.0, has optimum working temperature range of about 50 deg.C in 100mM tris-HCl buffer solution (pH 9.0), is strongly inhibited with HgCl2 and has a molecular weight of about 64,500+ or -5,000 (gel- filtration).

2. 发明专利

JPH08205861A NEW PYRANOSE OXIDASE, PYRANOSE OXIDASE GENE, NEW RECOMBINANT DNA AND PRODUCTION OF PYRANOSE OXIDASE 失效
公开(公告)号：JPH08205861A
公开(公告)日：1996-08-13
申请号：JP12483595
申请日：1995-05-24
申请人： KIKKOMAN CORP
发明人： NISHIMURA IKUKO , OKADA KIMIHARU , NANBARA TOMOYUKI , KAWAI GENSHIRO , KOYAMA TAIJI , SUZUKI MASARU
IPC分类号： C12N15/09 , C12N1/21 , C12N9/04 , C12N15/36 , C12N15/53 , C12R1/01 , C12R1/19
摘要： PURPOSE: To obtain a pyranose oxidase useful for assaying 1,5-anhydro-D-glucitol as a diagnosing marker for glucose in foods or body fluid etc., or diabetes. CONSTITUTION: This pyranose oxidase is obtained by culturing e.g. Escherichia coli XL1-Bluc (pPRME 10) (FERM BP-4831), a transformant produced by transformation of Escherichia coli with recombinant plasmid DNApPRME 10 containing a pyranose oxidase gene derived from Coriolus versicolor)ps4a (MAFF 420002), and has the following physicochemical properties: (1) capable of oxidizing glucose to form glucosone; (2) optimum pH value: 7-7.5, being stable at pH4.0-8.0; (3) optimum temperature: 50 deg.C or so; (4) also capable of acting on galactose, L-sorbose, D-xylose, and 1,5-anhydro-D-glucitol; (5) molecular weight: about 290000 (gel filtration method).

3. 发明专利

JP2004024012A Method for monitoring condition of food 有权
公开(公告)号：JP2004024012A
公开(公告)日：2004-01-29
申请号：JP2002180809
申请日：2002-06-21
申请人： Kikkoman Corp , キッコーマン株式会社
发明人： TATSUMI HIROKI , FUKUDA MASARU , NANBARA TOMOYUKI
IPC分类号： G01N21/78 , C12Q1/34 , C12Q1/66 , G01N33/02
摘要： PROBLEM TO BE SOLVED: To provide a method for monitoring conditions of a food product, especially the degree of heat treatment of the food product, the degree of sterilizing treatment of the food product or freshness of the food product by using an enzyme activity in the food product as an index.
SOLUTION: 1. The method for monitoring the conditions of the food product comprises the following two steps. (1) a step of bringing the food product or its extract into contact with β-galactosidase as a substrate of (first step) and (2) a step of detecting a reaction product produced by the first step and thereby assaying the β-galactosidase activity in the food product (second step). 2. The method for monitoring the conditions of the food product comprises the following two steps. (3) a step of bringing the food product or its extract into contact with a luciferin galactoside, luciferase, adenosine 5'-triphosphae (ATP) and a metal ion (first step) and (4) a step of detecting the light emission produced by the first step and thereby assaying the β-galactosidase activity in the food product (second step). 3. A luciferin derivative, the luciferase, ATP and the metal ion are contained in a reagent for monitoring the conditions of the food product.
COPYRIGHT: (C)2004,JPO

4. 发明专利

JP2002186498A METHOD FOR DETECTING LACTOBACILLUS HETEROHIOCHII 失效
公开(公告)号：JP2002186498A
公开(公告)日：2002-07-02
申请号：JP2000389775
申请日：2000-12-22
申请人： KIKKOMAN CORP
发明人： KABUTOMORI TADAMICHI , FURUKI YOSHITAKA , NANBARA TOMOYUKI
IPC分类号： C12G3/02 , C12Q1/66 , C12R1/01
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting Lactobacillus heterohiochii in Sake, relating to a conventional method using the ATP bioluminescence method, by the use of neither a special filter for catching the bacteria, special medium for growing the bacteria nor a special bioluminescence image analytic system, and by the use of a general-purpose luminometer with no need of any high-level skill, intuition and experience. SOLUTION: This method for detecting Lactobacillus heterohiochii comprises the steps of diluting a Sake or a semi-finished product thereof with sterile water to decrease its alcohol concentration, directly culturing the diluted product, subjecting the resultant culture solution to microorganismal cytolysis, adding a luciferin/luciferase luminescent reagent to the resultant solution, and measuring the level of luminescence emanated from the solution.

5. 发明专利

JPH09234063A NEW ALPHA-GLUCOSIDASE AND ITS PRODUCTION 失效
公开(公告)号：JPH09234063A
公开(公告)日：1997-09-09
申请号：JP34240096
申请日：1996-12-09
申请人： KIKKOMAN CORP
发明人： NANBARA TOMOYUKI , OKADA KIMIHARU , SUZUKI MASARU
IPC分类号： C12N9/26 , C12R1/38
摘要： PROBLEM TO BE SOLVED: To obtain a new α-glucosidase acting on maltooligosaccharide, etc., having a degree of polymerization below that of maltotetraose but entirely inert to soluble starch, and useful as a coordinate enzyme for α-amylase activity assay and quantitative analysis of chlorine ions, as an enzyme for assaying and scavenging maltose, etc., or as an enzyme for industrial production of glucose and fructose, etc., from oligosaccharides. SOLUTION: This enzyme which is capable of hydrolyzing α-glucoside linkages to produce α-D-gluocose, and obtained by cultivation of Bacillus sp. KS-108a (FERM BP-5337) etc., is an α-gluocosidase having the following physicochmical properties: (1) having substrate specificity to maltoligosaccharides having a degree of polymerization below that of maltotetraose, and p- nitrophenyl-α-D-glucopyranoside etc.; (2) having the optimum pH at 6.0 to 9.0; (3) having the optimum action temperature at 52 to 55 deg.C.

6. 发明专利

JP2003339397A Method for detection of microorganism 审中-公开
标题翻译：检测微生物的方法
公开(公告)号：JP2003339397A
公开(公告)日：2003-12-02
申请号：JP2002149910
申请日：2002-05-24
申请人： Kikkoman Corp , キッコーマン株式会社
发明人： TATSUMI HIROKI , FUKUDA MASARU , NANBARA TOMOYUKI
IPC分类号： G01N33/48 , C12Q1/04
摘要： PROBLEM TO BE SOLVED: To provide a method for detection of microorganisms rapidly and accurately detecting specific microorganisms for a case in which a small number such as several cfus of the specific microorganisms exist in tens of millimeters or grams or more of foods or materials for foods. SOLUTION: The objective microorganisms are rapidly and accurately detected by culturing the foods or the materials for foods in a condition proliferating the objective microorganisms and then culturing a part of the culture products in a condition selectively proliferating the objective microorganism, and then measuring an enzyme which is a marker for the objective microorganisms, in high sensitivity. COPYRIGHT: (C)2004,JPO
摘要翻译：要解决的问题：提供一种微生物检测方法，用于在几十毫克或更多个食物中存在几个特定微生物数量少的存在的情况下，特异性微生物的检测或食品材料解决方案：通过在增加目标微生物的条件下培养食品或食品材料，然后在选择性增殖目标微生物的条件下培养一部分培养产物，快速准确地检测目标微生物，然后测量作为目标微生物的标记物的酶，具有高灵敏度。版权所有（C）2004，JPO

7. 发明专利

JPH10286092A ALPHA-GLUCOSIDASE GENE, NEW RECOBMINED DNA AND PRODUCTION OF ALPHA-GLUCOSIDASE 失效
公开(公告)号：JPH10286092A
公开(公告)日：1998-10-27
申请号：JP11177897
申请日：1997-04-15
申请人： KIKKOMAN CORP
发明人： NANBARA TOMOYUKI , KOYAMA TAIJI , SUZUKI MASARU
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N9/26 , C12N15/00 , C12R1/07 , C12R1/19
摘要： PROBLEM TO BE SOLVED: To obtain the subject new α-glucosidase gene encoding a protein having a specific amino acid sequence and useful for producing α-glucosidase small in a Km value, excellent in substrate specificity and stability and used for measuring the activity of α-amylase, etc. SOLUTION: This new α-glucosidase gene encodes a protein having an amino acid sequence of the formula or a protein having the amino acid sequence of the formula wherein one or more amino acids are deleted, replaced or added and having an α-glucosidase activity, and is used for efficiently producing an α-glucosidase small in a Km value, excellent in substrate specificity and stability, and useful as a coupling enzyme on the measurement of the activity of α-amylase and on the determination of chloride ion or as an enzyme for determining or deleting maltooligosaccharide. The gene is obtained by cloning the chromosomal DNA of Bacillus s.p. KS-108a (FERM BP-5337).

8. 发明专利

JPH10108692A HIGHLY SENSITIVE QUANTIFICATION OF D-SORBITOL AND GALACTITOL 失效
公开(公告)号：JPH10108692A
公开(公告)日：1998-04-28
申请号：JP27856096
申请日：1996-10-01
申请人： KIKKOMAN CORP
发明人： NANBARA TOMOYUKI , ONO TAKESHI
IPC分类号： C12Q1/26 , C12Q1/32 , C12Q1/48
摘要： PROBLEM TO BE SOLVED: To quickly quantify the level of minute amounts of D-sorbitol or galactitol or their mixture in a sample such as of blood, tissue, body fluid, food or the like in a simple operation and in high sensitivity. SOLUTION: First, a dehydrogenase reactive to D-sorbitol and/or galactitol in the presence of NAD is added to a sample to carry out a reaction. Secondly, the resultant NADH is phosphorylated by the use of NADH kinase to form NADPH. Subsequently, cycling reactions are conducted by using an enzyme or electron carrier capable of oxidizing the NADPH into NADP and that capable of reducing NADP into NADPH, and the change in substrate consumed or product formed in the cycling reactions is detected, thus quantifying the D-sorbitol and/or galactitol in high sensitivity.

9. 发明专利

JPH09201194A NEW ALPHA-GLUCOSIDASE AND ITS PRODUCTION 失效
公开(公告)号：JPH09201194A
公开(公告)日：1997-08-05
申请号：JP3117096
申请日：1996-01-26
申请人： KIKKOMAN CORP
发明人： NANBARA TOMOYUKI , OKADA KIMIHARU , SUZUKI MASARU
IPC分类号： C12N9/26 , C12R1/07
摘要： PROBLEM TO BE SOLVED: To produce a new α-glucosidase, capable of hydrolyzing an α-glucoside bond and providing α-D-glucose and useful for measurement, etc., of α-amylase activities by culturing a strain, belonging to the genus Bacillus and having the ability to produce the specific α-glucosidase. SOLUTION: This new α-glucosidase has functions to hydrolyze an α-glucoside bond and produce α-glucose, is capable of acting on maltooligosaccharide having the polymerization degree ranging from maltose to maltooctaose, a p- nitrophenyl-α-D-glucopyranoside, a p-nitrophenyl-α-D-maltoside, a p-nitrophenyl-α- D-maltopentaoside and nigerose without acting on isomaltose and kojibiose, has 6.5-7.0 optimum pH, 50-55 deg.C optimum temperature and about 42,000±5000 (measured by a gel filtration method) and is useful for measurement, etc., of α-amylase activities. The enzyme is obtained from a cultured product of a strain belonging to the genus Bacillus and having the ability to produce the α-glucosidase.

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