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1. 发明专利

JPS62265993A MONOCLONAL ANTIBODY AGAINST VIBRIO CHOLERAE, HYBRIDOMA PRODUCING SAME AND DETECTION OF VIBRIO CHOLERAE USING SAME 失效
公开(公告)号：JPS62265993A
公开(公告)日：1987-11-18
申请号：JP11017786
申请日：1986-05-14
申请人： DENKA SEIKEN KK
发明人： MATSUDA JUNJI , GONDAIRA FUMIO , HIRANO MASARU , SOGA MASAO , SUGIYAMA JUNICHI , TERADA TOMOJI
IPC分类号： C07K14/195 , C07K14/005 , C07K14/41 , C07K16/00 , C07K19/00 , C12N5/00 , C12N5/10 , C12N15/00 , C12N15/02 , C12P21/00 , C12P21/08 , C12Q1/00 , C12R1/91 , G01N33/569 , G01N33/577
摘要： PURPOSE:To provide an antibody which is a monoclonal antibody against antigen b-factor of Vibrio cholerase and capable of detecting Ogawa-type Vibrio cholerae in high sensitivity. CONSTITUTION:A spleen cell of an animal immunized with Ogawa-type Vibrio cholerae is fused with a myeloma cell to obtain a hydridoma capable of producing a monoclonal antibody against antigen b-factor of Vibrio cholerae. The hybridoma is preferably hybridoma B-3-14 or A-1-1. The monoclonal antibody against antigen b-factor of Vibrio cholerae exhibits strong agglutination behavior to Ogawa-type Vibrio cholerae but no agglutination behavior to Inaba-type Vibrio cholerae.

2. 发明专利

JPS62265994A MONOCLONAL ANTIBODY AGAINST VIBRIO CHOLERAE, HYBRIDOMA PRODUCING SAME AND DETECTION OF VIBRIO CHOLERAE USING SAME 失效
公开(公告)号：JPS62265994A
公开(公告)日：1987-11-18
申请号：JP11017886
申请日：1986-05-14
申请人： DENKA SEIKEN KK
发明人： MATSUDA JUNJI , GONDAIRA FUMIO , HIRANO MASARU , SOGA MASAO , SUGIYAMA JUNICHI , TERADA TOMOJI
IPC分类号： C07K14/195 , C07K14/005 , C07K14/41 , C07K16/00 , C07K19/00 , C12N5/00 , C12N5/10 , C12N15/00 , C12N15/02 , C12P21/00 , C12P21/08 , C12Q1/00 , C12R1/91 , G01N33/569 , G01N33/577
摘要： PURPOSE:To provide an antibody which is a monochlonal antibody against antigen c-factor of Vibrio chlerae and capable of detecting Inaba-type Vibrio cholerae in high sensitivity. CONSTITUTION:A spleen cell of an animal immunized with Inaba-type Vibrio cholerae is fused with a myeloma cell to obtain a hybridoma capable of producing a monoclonal antibody against antigen c-factor of Vibrio cholerae. The hybridoma is preferably hybridoma 41-1 or 22-4. The monoclonal antibody against antigen c-factor of Vibrio cholerae exhibits only weak agglutination behavior to Ogawa-type Vibrio cholerae but exhibits strong agglutination behavior to Inaba-type Vibrio cholerae.

3. 发明专利

JP2003238599A REAGENT FOR DETECTING BACILLUS ANTHRACIS 审中-公开
公开(公告)号：JP2003238599A
公开(公告)日：2003-08-27
申请号：JP2002031199
申请日：2002-02-07
申请人： DENKA SEIKEN KK
发明人： NIIHORI YOMIKO , GONDAIRA FUMIO , SUGIYAMA JUNICHI , MAKINO SOICHI
IPC分类号： G01N33/569 , C07K16/12 , C12Q1/04
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting Bacillus anthracis using an antibody recognizing the capsule, microbial cell and spore possessed by the Bacillus anthracis, and to provide a kit therefor. SOLUTION: An antibody composition containing the antibody against the Bacillus anthracis contains at least one of an antibody against the capsule of the Bacillus anthracis, an antibody against the spore of the Bacillus anthracis, and an antibody against the Bacillus anthracis free from the capsule and the spore. COPYRIGHT: (C)2003,JPO

4. 发明专利

JPH05172816A MEASURING METHOD FOR ANTIGEN 失效
公开(公告)号：JPH05172816A
公开(公告)日：1993-07-13
申请号：JP13713591
申请日：1991-05-13
申请人： DENKA SEIKEN KK
发明人： GONDAIRA FUMIO , YOSHIMURA RYUJI , SUGIYAMA JUNICHI
IPC分类号： G01N33/543
摘要： PURPOSE:To enable the highly accurate measurement of an antigen in a short time by applying force to an obtained sediment in another direction different from the sedimentation direction upon applying centrifugal force, followed by measuring its dimensional change. CONSTITUTION:A specimen, in which the antigen to be measured is likely to be contained, is reacted with a carrier, in which the corresponding antibody is contained in a solid phase, directly or dilutedly. An antigen-antibody reaction can be performed under a normal condition. The resulted reactant is forcibly sedimented by a centrifugal operation to form a sediment of a reactant. The condition for the centrifugal operation is not preferably restricted as far as the reactant can be sedimented, for example about ten minutes at 1200 rpm. Next, an external force is applied to the obtained sediment in another direction different from the sedimentation direction upon applying the centrifugal force, followed by measuring the dimensional change of the sediment. Although the sediment maintains the pellet-like shape if an antigen-antibody reaction has taken place, it can be observed that the carrier particle flows in a tear-like fashion if no antigen-antibody reaction has taken place.

5. 发明专利

JPH11248709A ANTIGEN MEASURING METHOD 失效
公开(公告)号：JPH11248709A
公开(公告)日：1999-09-17
申请号：JP5323898
申请日：1998-03-05
申请人： DENKA SEIKEN KK
发明人： NAKAOMI YASUO , OZAWA KENSUKE , GONDAIRA FUMIO
IPC分类号： G01N33/543
摘要： PROBLEM TO BE SOLVED: To measure a low concentration of antigen in a sample with high sensitivity by bringing about a reaction between an antigen present in a sample solution and an antibody carried by magnetic particles, gathering the magnetic particles, suspending them in a small amount of liquid, mixing carried non-magnetic insoluble particles into reaction, and measuring the degree of agglutination of the obtained reaction mixture. SOLUTION: An antigen to be an object to be measured is a microorganism or an antigen derived from a microorganism and is tubercle bacillus, staphylococcus, etc. There arc organic high-molecular matter and inorganic micro powder containing magnetic bodies as magnetic particles. There are organic high-molecular matter and inorganic micro powder as non-magnetic insoluble particles. In measurement, a sample solution and a magnetic particle liquid are mixed to bring about a reaction between an antigen present in the sample solution and an antibody carried by magnetic particles. After achieving equilibrium in the reaction. a magnetic field is applied to gather not-yet-reacted magnetic particles and carried magnetic particles. The gathered magnetic particles are suspended in a small amount of liquid to concentrate the antigen selectively. After the non-magnetic insoluble particle liquid carrying the antibody is mixed with the suspension to bring about a reaction, the degree of agglutination is measured.

6. 发明专利

JPS62267298A MONOCLONAL ANTIBODY OF ANTI-VIBRIO CHOLERAE, HYBRIDOMA PRODUCING SAME AND METHOD FOR DETECTING VIBRIO CHOLERAE USING SAID MONOCLONAL ANTIBODY 失效
公开(公告)号：JPS62267298A
公开(公告)日：1987-11-19
申请号：JP11017686
申请日：1986-05-14
申请人： DENKA SEIKEN KK
发明人： MATSUDA JUNJI , GONDAIRA FUMIO , HIRANO MASARU , SOGA MASAO , SUGIYAMA JUNICHI , TERADA TOMOJI
IPC分类号： C07K14/195 , C07K14/005 , C07K14/41 , C07K16/00 , C12N5/00 , C12N5/10 , C12N15/00 , C12N15/02 , C12P21/00 , C12P21/08 , C12R1/91 , G01N33/569 , G01N33/577
摘要： PURPOSE:To detect Vibrio cholerae in good sensitivity, by subjecting a splenic cell of an animal immunized against Vibria cholerae and a myeloma cell to cell fusion to give a hybridoma and producing the titled antibody by the use of the hybridoma. CONSTITUTION:Pellets of a splenic cell of an animal (e.g. mouse, etc.) immunized against Vibrio cholerae and pellets of the myeloma cell of the animal are suspended in a solution containing a fusing agent (e.g. polyethylene glycol, etc.), blended and incubated to give a hybridoma, which is cloned by limiting dilution method to give a hybridoma producing a monoclonal antibody of anti- Vibrio cholerae. Then, the hybridoma is cultivated, the aimed monoclonal antibody is obtained from the supernatant liquid of the culture mixture or the hybridoma is inoculated into an animal and the aimed monoclonal antibody is obtained from the ascites and purified by the conventional procedure. The antibody is reacted with a test specimen and a formed antigen-antibody complex is detected so Vibrio cholerae is detected.

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