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JP4445597B2 Cell suspension processing method and its implementation apparatus 有权
标题翻译：空值
公开(公告)号：JP4445597B2
公开(公告)日：2010-04-07
申请号：JP1540699
申请日：1999-01-25
申请人：フレセニウス・アクチエンゲゼルシャフト
发明人：ヴォルフガンク・ビーゼル
IPC分类号： A61M1/02 , A61M1/36 , B04B5/04
CPC分类号： B04B5/0442 , A61M1/3692 , A61M1/3693 , A61M1/3696 , A61M2202/0429 , B04B2005/045 , A61M2202/0071
摘要： A cell suspension preparation method comprises dilution of a concentrated cell fraction, after discharge from a centrifugal separation unit, with a physiological solution. An Independent claim is also included for the corresponding apparatus, comprising a centrifuge (1) which includes a separation unit (2) with an annular channel and which has connections for a suspension supply line (5), a concentrated cell fraction discharge line (9) and a residual suspension component discharge line (13), a dilution unit (22) being provided for diluting the cell fraction delivered by the concentrated cell fraction discharge line (9). PREFERRED FEATURES:- The cells are concentrated to a hematocrit value of 60-98. The physiological solution is a Ringer solution. The dilution unit (22) comprises a diluent container (15) with a feed line (16), including a feed pump (17), connected to the concentrated cell fraction discharge line (9). The concentrated cell fraction discharge line (9) includes a concentrate pump (11) which withdraws the cell fraction from the separation unit (2) and which is located downstream of the feed line (16) connection point (23). The dilution unit has a control unit (19) for adjusting the feed rate of the diluent feed pump (17).
摘要翻译：细胞悬浮液制备方法包括在离心分离单元排出之后用生理溶液稀释浓缩的细胞级分。还包括用于相应装置的独立权利要求，其包括离心机（1），其包括具有环形通道的分离单元（2），并且具有用于悬浮液供应管线（5），浓缩池单元排出管线（9））和残留悬浮成分排出管线（13），稀释单元（22）用于稀释由浓缩的细胞部分排出管线（9）输送的细胞部分。优点特点： - 细胞浓缩至血细胞比容值为60-98。生理溶液是林格溶液。稀释单元（22）包括具有进料管线（16）的稀释剂容器（15），其包括连接到浓缩的细胞馏分排出管线（9）的进料泵（17）。浓缩的细胞馏分排出管线（9）包括从分离单元（2）中抽出细胞部分并且位于进料管线（16）连接点（23）下游的浓缩物泵（11）。稀释单元具有用于调节稀释剂供给泵（17）的进料速率的控制单元（19）。

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