会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 8. 发明专利
    • Method for discriminating cell
    • 分辨细胞的方法
    • JP2010022224A
    • 2010-02-04
    • JP2008184710
    • 2008-07-16
    • Seitai Kagaku Kenkyusho:Kk株式会社生体科学研究所
    • SHIGEMATSU AKIYO
    • C12Q1/02G01N33/48
    • PROBLEM TO BE SOLVED: To provide a method for discriminating cells, which discriminates cells in a cell division S stage during a specific short time from cells except them in a cell small mass of examination object so as to observe cell division ability of examination object.
      SOLUTION: The method for discriminates cells in a cell division S stage in a time of contact with an animal serum containing [2-14C] thymidine comprises immersing a cell small mass being an examination object in an animal serum containing [2-14C] thymidine at a normal temperature for 2-5 minutes, then immediately, bringing the cell small mass into contact with a fixing solution of an in vivo tissue sample on a slide glass for 2-5 minutes to stick the cell small mass on the slide glass, then immersing the slide glass, on which the cell small mass is stuck, in a picric acid-containing solution for ≥10 minutes, after washing in water, detecting the distribution of 14C.
      COPYRIGHT: (C)2010,JPO&INPIT
    • 解决问题的方案:提供一种辨别细胞的方法,其特征在于,在特定短时间内将细胞分化S期的细胞与细胞小体积的检查对象之外的细胞区分开,以观察细胞分裂能力 检查对象 解决方案:与含有[2-14C]胸苷的动物血清接触的细胞分裂S期中鉴别细胞的方法包括将作为检查对象的细胞小块浸入含有2- [ 14C]胸苷在常温下2-5分钟,然后立即使细胞小体积与载玻片上的体内组织样品的定影液接触2-5分钟,以将细胞块粘附在 然后在水中洗涤之后,将容纳细胞小块的载玻片浸入含苦味酸的溶液中≥10分钟,检测14C的分布。 版权所有(C)2010,JPO&INPIT