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51. 发明专利

JPH07147986A HUMAN PARVOVIRUS GENE, POLYPEPTIDE CODED WITH THE SAME AND USE 失效
公开(公告)号：JPH07147986A
公开(公告)日：1995-06-13
申请号：JP28101792
申请日：1992-09-24
申请人： DENKI KAGAKU KOGYO KK , DENKA SEIKEN KK
发明人： YAMAZAKI OSAMICHI , MATSUNAGA YASUKO , TAKEDA NAOKAZU , MATSUURA ZENJI , OGAWA HIROYUKI , SHIMIZU HIDEHARU , KAMATA KUNIO , KUROSAWA DAISUKE
IPC分类号： G01N33/53 , C07K14/015 , C07K16/00 , C12N1/21 , C12N5/10 , C12N15/09 , C12P21/02 , C12Q1/68 , C12Q1/70 , C12R1/19 , C12R1/91 , G01N33/569
摘要： PURPOSE:To provide the gene coding a human parvovirus (HPV) antigen protein, enabling to massively produce the antigen protein, capable of being utilized for the high sensitive measurement of the HPV and the HPV-resistant antibody in a specimen, and useful for the diagnoses of HPV-originated diseases, etc. CONSTITUTION:The serum of a patient infected with infectious erythema is diluted with a phosphate-buffered physiological saline (pH: 7.2), mixed with proteinase K and sodium dodecyl sulfate, subjected to their reaction at 37 deg.C for 1hr, mixed with phenol, chloroform and isoamyl alcohol, and subsequently centrifuged to recover the supernatant liquid. The supernatant liquid is added to ethanol and subsequently allowed to stand at -20 deg.C to obtain the precipitates of human parvovirus DNA. The DNA is multiplied by a PCR method, and then treated with a restriction enzyme to obtain the objective human parvovirus gene enabling the mass production of the human parvovirus antigen protein and enabling the measurement the human parvovirus or the human parvovirus- resistant antibody in a specimen in high sensitivity.

52. 发明专利

JPH05172816A MEASURING METHOD FOR ANTIGEN 失效
公开(公告)号：JPH05172816A
公开(公告)日：1993-07-13
申请号：JP13713591
申请日：1991-05-13
申请人： DENKA SEIKEN KK
发明人： GONDAIRA FUMIO , YOSHIMURA RYUJI , SUGIYAMA JUNICHI
IPC分类号： G01N33/543
摘要： PURPOSE:To enable the highly accurate measurement of an antigen in a short time by applying force to an obtained sediment in another direction different from the sedimentation direction upon applying centrifugal force, followed by measuring its dimensional change. CONSTITUTION:A specimen, in which the antigen to be measured is likely to be contained, is reacted with a carrier, in which the corresponding antibody is contained in a solid phase, directly or dilutedly. An antigen-antibody reaction can be performed under a normal condition. The resulted reactant is forcibly sedimented by a centrifugal operation to form a sediment of a reactant. The condition for the centrifugal operation is not preferably restricted as far as the reactant can be sedimented, for example about ten minutes at 1200 rpm. Next, an external force is applied to the obtained sediment in another direction different from the sedimentation direction upon applying the centrifugal force, followed by measuring the dimensional change of the sediment. Although the sediment maintains the pellet-like shape if an antigen-antibody reaction has taken place, it can be observed that the carrier particle flows in a tear-like fashion if no antigen-antibody reaction has taken place.

53. 发明专利

JPH05172812A MEASUREMENT OF COMPLEMENT BONDING ANTIBODY QUANTITY AND REAGENT 失效
公开(公告)号：JPH05172812A
公开(公告)日：1993-07-13
申请号：JP21300191
申请日：1991-07-31
申请人： DENKA SEIKEN KK
发明人： SEKINE SHIGERU
IPC分类号： G01N33/53 , G01N33/544 , G01N33/555
摘要： PURPOSE:To provide a method that is convenient to operate and allows the high-precision measurement of complement bonding antibodies in specimen, and to obtain reagent for its purpose. CONSTITUTION:Specimen is reacted on complement bonding antigens and complements, complement activating capsules are added thereto and the amount of filled material in the capsules which is changed in suspensibility or scattering due to capsule destruction or isolated from the capsules, to provide a method of measuring the amount of complement bonding antibodies for quantifying the amount of complement bonding antibodies in the specimen. The complement bonding antigens and the complements are mixed together and dried to obtain reagent for use in the measurement of the amount of complement bonding antibodies with the complement, activating capsules.

54. 发明专利

JPH0545906B2 失效
公开(公告)号：JPH0545906B2
公开(公告)日：1993-07-12
申请号：JP22593687
申请日：1987-09-09
申请人： TOSHIBA KK , DENKA SEIKEN KK
发明人： IMAI TOSHIAKI , HEIJI TAKASHI
IPC分类号： G01N21/75

55. 发明专利

JPH05163161A VACCINE FOR RHINOVACCINATION 失效
公开(公告)号：JPH05163161A
公开(公告)日：1993-06-29
申请号：JP35211891
申请日：1991-12-13
申请人： DENKA SEIKEN KK
发明人： OBATA AKIRA , IKUSHIMA KOICHIRO
IPC分类号： A61K9/08 , A61K39/00 , A61K39/145 , A61K39/39 , A61K47/36
摘要： PURPOSE:To provide the subject vaccine containing hyaluronic acid, etc., effective for increasing the production of secretory antibody and circulating antibody, having antibody-producing function and useful for the prevention of influenza such as respiratory tract infection diseases. CONSTITUTION:The objective vaccine (preferably influenza vaccine) contains hyaluronic acid or its salt having a molecular weight of 50,000-8,000,000 (at a concentration of preferably 0.001-3.0w/v% in the vaccine).

56. 发明专利

JPH04117299A DETECTION OF BACTERIA 失效
公开(公告)号：JPH04117299A
公开(公告)日：1992-04-17
申请号：JP23540190
申请日：1990-09-05
申请人： DUSKIN CO LTD , DENKA SEIKEN KK
发明人： ISHIDATE MASAKAZU , KOYAMA TARO , YUGAWA HIROSHI , YOSHIMURA RYUJI , SUGIYAMA JUNICHI , ISHIGURO HITOSHI
IPC分类号： C12Q1/00 , C12Q1/04
摘要： PURPOSE:To simply, in high sensitivity detect bacteria in a short time by incubating bacteria in a medium containing both trypticase soy agar medium and broth-added agar medium followed by superposing a reducing-type color developing reagent-contg. substrate on the medium and by observing the color tone change of said reagent. CONSTITUTION:Both trypticase soy agar medium and broth-added agar medium are added to purified water, and sodium pyruvate and L-glutamine as bacteria proliferation-promotive substances are added to the above system followed by hot sterilization at 121 deg.C for 15min. The resultant system is aliquoted onto laboratory dishes, left to stand under cooling and solidified. Thence, human fingers are rubbed on the resulting agar medium to collect bacteria, which is then incubated at 37 deg.C for 8hr, and a reducing-type color developing reagent [e.g. 3-(p-iodophenyl)-2-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]-contg. substrate is superposed on the agar medium and the resulting color tone change of said reagent is observed, thus detecting the bacteria.

57. 发明专利

JPH01240860A IMMUNOLOGICAL ANALYSIS 失效
公开(公告)号：JPH01240860A
公开(公告)日：1989-09-26
申请号：JP7000088
申请日：1988-03-23
申请人： TOSHIBA CORP , DENKA SEIKEN KK , GREEN CROSS CORP
发明人： MUTSUKAWA KYUJI , TAKIGUCHI YASUKO , ISHIMORI YOSHIO , SEKINE SHIGERU , MOTODA SHOSAKU , MATSUDA HIROSHI , INUI YUICHIRO
IPC分类号： G01N33/544
摘要： PURPOSE:To inhibit non-specific reaction, by making a non-specific anti- resolution substance of a ribosome coexist in a reaction solution in an immunological analysis using ribosome sealed up with a label substance having an antibody immobilized on the surface thereof. CONSTITUTION:A ribosome with phospholipid or glycolipid as composition having an antibody or a part thereof immobilized on the surface thereof and being sealed up with a label substance is brought into contrast with a reagent for immunological analysis. Here, a blocking agent is added to a reaction solution to prevent a non-specific resolution of the ribosome. This blocking agent employs those to which is bonded a hetero-philic antibody in a sample replacing an antibody on the ribosome, hence, a fraction of immunoglobulin derived from species the same as the antibody or a part thereof and belonging to the same class thereof immobilized on the ribosome, for example, the one obtained from an animal not sensitized with a specified antigen. This enables inhibiting of a non-specific reaction in an immunological analysis thereby achieving a highly accurate analysis.

58. 发明专利

JPH01144972A CULTIVATION AND COLLECTION OF PATHOGENIC STRAIN OF CIZZAR' S DISEASE 失效
公开(公告)号：JPH01144972A
公开(公告)日：1989-06-07
申请号：JP30302787
申请日：1987-11-30
申请人： DENKA SEIKEN KK
发明人： SHIBUYA TOYOHIKO , FUJIWARA AKIRA , MOTODA SHOSAKU
IPC分类号： C12N1/20 , C12R1/01 , C12R1/07
摘要： PURPOSE:To collect the title strain useful as a detection reagent for an antibody against the pathogen of Cizzar's disease which is an infectious disease of rabbit, etc., by inoculating the pathogen of Cizzar's disease (Bacillus piliformis) in amniotic cavity, allantoic cavity or yolk sac of an embryonated hen egg, proliferating the call and collecting the chorioallantoic fluid or amniotic fluid of the embryonated hen egg. CONSTITUTION:Pathogen of Cizzar's disease separated from the hepatic emulsion of infected animal is inoculated in amniotic cavity, allantoic cavity or yolk sac of an embryonated hen egg and proliferated in the embryonated hen egg by incubating the inoculated embryonated hen egg at 30-38 deg.C for 7 days. The chorioallantoic fluid or amniotic fluid of the embryonated hen egg is collected, inactivated with formalin and centrifuged at 2,000rpm for 10min. The precipitate is redispersed in a phosphate-buffered saline water at a specific concentration to achieve the cultivation and collection of the pathogen of Cizzar's disease in high purity.

59. 发明专利

JPH0113525B2 失效
公开(公告)号：JPH0113525B2
公开(公告)日：1989-03-07
申请号：JP11053782
申请日：1982-06-26
申请人： TOSHIBA KK , DENKA SEIKEN KK
发明人： MINEKANE TOMIJI , TAMURA EMIKO , TAKEUCHI KUNIO
IPC分类号： G01N1/00 , B01L3/00 , G01N1/10 , G01N33/48

60. 发明专利

JPS6336150A QUANTITATIVE DETERMINATION OF SPECIFIC IMMUNE GLOBULIN SPECIES ANTIBODY 失效
公开(公告)号：JPS6336150A
公开(公告)日：1988-02-16
申请号：JP17757086
申请日：1986-07-30
申请人： DENKA SEIKEN KK
发明人： IWASE ISAO , SATO SEIYA , KAWAMATA OSAMU
IPC分类号： G01N33/53
摘要： PURPOSE:To measure plural antibodies with one kind of antibody support by bringing a sample contg. the specific immune globulin species antibody to a known antigen, 1st antibody to said antibody, 2nd antibody to the 1st antibody and the known antigen into reaction with each other. CONSTITUTION:The sample contg. the specific immune globulin species antibody to the known antigen, the 1st antibody to said specific immune globulin species antibody,and the 2nd antibody fixed to the fixing support for the 1st antibody are first brought into reaction with each other. Then, the specific immune globulin species antibody to be quantitatively determined is fixed via the 1st antibody and the 2nd antibody to the fixing support. The antigen complex formed in the above-mentioned manner and the known antigen mentioned above are brought into reaction with each other and the quantity of the known antigen conjugated thereto is measured, by which the quantity of the specific immune globulin species antibody in the sample can be measured. Various specific immune globulin species antibodies are quantitatively determinable if the 1st antibody is changed to various antibodies.

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