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31. 发明专利

JPS58105052A SIMPLE METHOD OF TWO-DIMENTIONAL ELECTROPHORESIS 失效
公开(公告)号：JPS58105052A
公开(公告)日：1983-06-22
申请号：JP20372881
申请日：1981-12-18
申请人： HITACHI LTD
发明人： ITOU MICHIO , YOSHIDA MOTOKO
IPC分类号： G01N27/26 , G01N27/447
摘要： PURPOSE:To enable the substantial simplification of an operation of separating electrophoresis, by using the same plane-shaped gel for one-dimentional and two- dimentional electro-phoresis without distinguishing gels used for these two stages of electrophoresis from each other, and thereby enabling the handling of the gel together with a hard substrate. CONSTITUTION:One surface of a glass plate 1 is processed by methacryl oxypropyltrimethoxysilane, and thereby a plane gel 2 having the concentration gradient of acrylamide of 4-20% and containing potassium chloride is prepared. A cut 3 is made in the low-concentration part of the gel and a serum and amphorine, which is an amphoteric electrolyte, are given in it. Filter paper sheets 4 and 5 for liquid junction are connected to a negative electrode tank 9 and a positive electrode tank 10, and serum protein is separated according to the difference in an isoelectric point. A filter paper sheet impregnated with a buffer (A liquid) containing trishydroxymethyl-aminomethane and glycine is put on, the A liquid is made to permeate the plane gel 2, the filter paper sheet is removed, filter paper sheets 7 and 8 for liquid junction are connected to a positive electrode tank 11 and a negative electrode tank 12, and the serum protein is separated according to the difference in molecular weight.

32. 发明专利

JPS5853745A TWO DIMENSIONAL ELECTROPHORESIS DEVICE 失效
公开(公告)号：JPS5853745A
公开(公告)日：1983-03-30
申请号：JP15208581
申请日：1981-09-28
申请人： HITACHI LTD
发明人： YOSHIDA MOTOKO , ITOU MICHIO
IPC分类号： G01N27/26 , G01N27/447
摘要： PURPOSE:To enable simplifying of the process of a two dimensional electrophoresis method, by a method wherein one side of an acrylamide concentration gradient gel, used in two dimensional electrophoresis, is previously secured to a substrate. CONSTITUTION:A rodform acrylamide gel 1, whose one dimensional migration is completed, is extruded through a glass tube, and is placed in a recess in a low concentration side of a concentration gradient polyamide flat board gel 2 for two dimensional migration, which is placed on a cooling plate 6 and has one side secured to a substrate, so that air bubbles is prevented from entering. The opposite ends of the flat board gel 2 are liquid-connected to buffer 7 through filter papers 4 to perform a horizontal type electrophoresis. The flat board gel used herewith is prepared such that a glass plate, on which a silane coupling treatment is made, and a glass plate, on which no said treatment is made, are positioned facing and opposite to each other with a spacer between, and acrylamide monomer, in which a continuous change in concentration is produced, a cross linking agent, and a mixture solution of polymerization catalyst are injected between the spacer and the glass for polymerization to prepare a polymer.

33. 发明专利

JPS5410934A ALKALINE CELL 失效
公开(公告)号：JPS5410934A
公开(公告)日：1979-01-26
申请号：JP7571777
申请日：1977-06-27
申请人： HITACHI MAXELL , HITACHI LTD
发明人： YOKOYAMA KENICHI , KAWAKAMI AKIRA , KOTANI NOBORU , YOSHIDA MOTOKO , KUDOU TETSUICHI , OOHAYASHI HIDEHITO
IPC分类号： H01M2/08 , H01M6/04

34. 发明专利

JPS53142632A GAS ELECTRODE 失效
公开(公告)号：JPS53142632A
公开(公告)日：1978-12-12
申请号：JP5638577
申请日：1977-05-18
申请人： HITACHI LTD
发明人： YOSHIDA MOTOKO , KIKUCHI TAKESHI , OOHAYASHI HIDEHITO , KUDOU TETSUICHI
IPC分类号： H01M4/86

35. 发明专利

JPS53131437A ALKALINE BATTERY 失效
公开(公告)号：JPS53131437A
公开(公告)日：1978-11-16
申请号：JP4576377
申请日：1977-04-22
申请人： HITACHI MAXELL , HITACHI LTD
发明人： YOKOYAMA KENICHI , KAWAKAMI AKIRA , KOTANI NOBORU , YOSHIDA MOTOKO , KUDOU TETSUICHI , OOHAYASHI HIDEHITO
IPC分类号： H01M2/08 , H01M2/04 , H01M6/04

36. 发明专利

JPH10206387A METHOD AND DEVICE TO CONDENSE SAMPLE SOLUTION 失效
公开(公告)号：JPH10206387A
公开(公告)日：1998-08-07
申请号：JP1095397
申请日：1997-01-24
申请人： HITACHI LTD
发明人： TAKADA YASUAKI , SAKAIRI MINORU , NABESHIMA TAKAYUKI , YOSHIDA MOTOKO , KIMURA KOICHI
IPC分类号： G01N27/62 , G01N1/28 , G01N1/36 , G01N30/08
摘要： PROBLEM TO BE SOLVED: To condense the difficult-to-volatile substance contained in the solution by spraying and atomizing the sample solution introduced in a small pipe from a terminal of the small pipe by the gas injection flow, and recovering the ion derived from the sample solution by electrodes. SOLUTION: The sample solution is introduced in a small pipe 1. A terminal of the small pipe 1 is installed in the vicinity of a gas injection port 2 provided on a container 5, the gas introduced in the container 5 flows along the outside of the small pipe 1. When the gas is injected from the gas injection port 2 at the flow speed of about the sonic velocity, the sample solution is injected from the terminal of the small pipe 1, and atomized, and the sample substance in the fine droplet is taken out into the gas phase as the positive/negative ions. The substance is drifted in the direction of recovery electrodes 3a, 3b to which different voltage is applied by a power source 4a, and precipitated on its surface. The precipitated sample substance is recovered to obtain the solid sample substance, and the sample solution of high concentration can be generated by dissolving the sample substance in the solvent of arbitrary quantity.

37. 发明专利

JPH0980022A DATA PROCESSING METHOD IN MASS SPECTROMETER 失效
公开(公告)号：JPH0980022A
公开(公告)日：1997-03-28
申请号：JP23500195
申请日：1995-09-13
申请人： HITACHI LTD
发明人： TAKADA YASUAKI , SAKAIRI MINORU , NABESHIMA TAKAYUKI , HIRABAYASHI YUKIKO , YOSHIDA MOTOKO , KOIZUMI HIDEAKI
IPC分类号： G01N27/62 , G01N27/447
摘要： PROBLEM TO BE SOLVED: To increase the reliability of an analyzed result using a capillary electrophoretic mass spectrometer by a method wherein a plurality of analytical operations are performed under the same analytical condition, the electrophoretic times of at least one substance are compared and the time base of the obtained analyzed result is corrected. SOLUTION: Ion electropherograms obtained, in a first analytical operation and a second analytical operation, of ions whose value m/z obtained by dividing the valence of ions by the molecular weight of ions is (x) are designated respectively as A and B. A peak caused by the ions to which attention is to be paid is designated as P1 , the peak P1 is observed at a time t1 in the electropherogram A, and it is observed at a time t1 in the electropherogram B. When the time base of the electropherogram B is corrected by making use of the peak P1 in the electropherogram A as a reference, the peak P1 in the electropherogram B is observed at the time t1 ' although it should be observed at the time t1 . As a result, the time base in the electropherogram B is multiplied by t1 /t1 ' so as to obtain a time base after it has been corrected in the electropherogram B. Thereby, an apparent time base in the electropherogram A agrees with that in the eletropherogram B, and the elecropherograms A, B can be compared directly.

38. 发明专利

JPH0886772A CAPILLARY ELECTROPHORETIC DEVICE 失效
公开(公告)号：JPH0886772A
公开(公告)日：1996-04-02
申请号：JP21978494
申请日：1994-09-14
申请人： HITACHI LTD
发明人： YOSHIDA MOTOKO , MURAYAMA SEIICHI , TAKI MAMORU
IPC分类号： G01N27/447
摘要： PURPOSE: To enable trace components to be detected without such processes as fluorescent labeling or derivatization. CONSTITUTION: A beam emitted from an UV light source 1 is focused at a slit position by a condensing lens 7, and a pinhole 2 is placed at a light-source focusing position to reduce the light source size to a capillary bore or less. The beam from the light source is converted into a parallel beam, which is then transmitted through a half mirror 10 and focused inside a capillary 4 by a condensing lens 9. A branching beam produced by the half mirror 10 is used as a reference. The branching beam and the beam transmitted through the capillary 4 are received by a receiver 5 and processed at a signal processor 6, from which the results of processing are then output. A diaphragm 3 for controlling the solid angle of the beam incident on the capillary is combined with the condensing lens 9. The beam incident on the capillary 4 is allowed to pass through only a space where a sample at the center of the capillary passes, while the beam received by the receiver without passing through the space where the sample passes is blocked.

39. 发明专利

JPH0674938A CELL WINDOW FORMING DEVICE FOR CAPILLARY 失效
公开(公告)号：JPH0674938A
公开(公告)日：1994-03-18
申请号：JP23092592
申请日：1992-08-31
申请人： HITACHI LTD
发明人： TAKI MAMORU , WATANABE YOSHIO , NAKAYAMA KANAE , YOSHIDA MOTOKO
IPC分类号： B01D57/02 , G01N27/447
摘要： PURPOSE:To form a cell window slit by removing a part of a capillary coating layer mechanically and accurately in a prescribed dimension. CONSTITUTION:A capillary 1 is fixed to a holding body 6, and the edges formed by adjusting clearance A between a pair of work bodies 5a and 5b in a prescribed slit width dimension by means of a spring 4, a support point 8, a screw- fitting part 10, a clearance adjusting presser screw 9 and so on, are pushed against a capillary 1 coating layer 2, and the edges of the pair of work bodies 5a and 5b are closed in an arrow direction, and a part of the capillary 1 coating layer 2 is removed mechanically in a prescribed dimension, and a slit can be formed.

40. 发明专利

JPH0574779B2 失效
公开(公告)号：JPH0574779B2
公开(公告)日：1993-10-19
申请号：JP1003484
申请日：1984-01-25
申请人： HITACHI LTD
发明人： YOSHIDA MOTOKO , ITO MICHIO , MAEDA KUNIHIRO
IPC分类号： G01N27/26 , B01D57/02 , G01N27/447

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