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21. 发明专利

JP2001112471A SUPPORT FOR IMMOBILIZING USE, METHOD FOR PRODUCING FERMENTED PRODUCT, AND THE RESULTANT FERMENTED PRODUCT 失效
公开(公告)号：JP2001112471A
公开(公告)日：2001-04-24
申请号：JP29420599
申请日：1999-10-15
申请人： SAPPORO BREWERIES , FUJI SPINNING CO LTD
发明人： UMEMOTO SHINGO , MAEHANE HIDEO , KAWAMURA YOSHIHIDE , ISHIKAWA EIKO , YABE HIROAKI
IPC分类号： C12C11/00 , C12M1/40 , C12N11/10
摘要： PROBLEM TO BE SOLVED: To obtain a support for immobilizing use, capable of attaining good fluidity inside a fluidized bed-type reactor through preventing the residence of gas components produced by fermentation, thereby enabling fermentation efficiency to be maintained high and stably. SOLUTION: This support 10 for immobilizing use is such as to be nearly spherical, provided with pores 12 on its surface and inside, to be packed in a bioreactor in such a condition as to carry microorganisms or the like thereon, 1.5-4.0 mm in average outer diameter, and 7.0-50.0 mm2 in the total area of the flat surface when its surface is assumed as flat surface; wherein the pores 12 lying open on its surface meet the following relationships (1) and (2): S1 =300 μm2 in open area).

22. 发明专利

JPH0956379A REGENERATION OF IMMOBILIZED LIPASE 失效
公开(公告)号：JPH0956379A
公开(公告)日：1997-03-04
申请号：JP23331895
申请日：1995-08-18
申请人： FUJI SPINNING CO LTD
发明人： KAWAMURA YOSHIHIDE , YABE HIROAKI
IPC分类号： C12N11/02 , C12N9/20 , C12N11/14
摘要： PROBLEM TO BE SOLVED: To regenerate the activities of an immobilized lipase capable of providing a liquid crystal raw material or a physiologically active substance by optical resolution or asymmetric synthesis by placing the immobilized lipase having remarkably reduced activities in ester transfer reaction as compared with the initial ones in a polar solvent containing a small amount of water. SOLUTION: Chitosan is dissolved in a 3.5% aqueous solution of acetic acid and the resultant solution is then dropped into a coagulating solution such as a 7% aqueous solution of sodium hydroxide to provide a regenerated granular porous chitosan carrier having 0.1mm average grain diameter. A diglycidyl compound is then added thereto to carry out cross-linking reaction and the resultant product is further reacted with stearoyl chloride to afford a cross-linked granular regenerated porous chitosan carrier. An aqueous solution of a lipase is subsequently added to the carrier to adsorb the lipase on the carrier. The resultant carrier is then washed with water to prepare an immobilized lipase, which is then filled in a column to carry out the ester transfer reaction. When the activities thereof are reduced to those without exceeding 1/10 of the initial ones, the immobilized lipase is placed in a polar solvent (e.g. acetone) containing 1-5% water to regenerate the enzymic activities. Thereby, the immobilized lipase is regenerated.

23. 发明专利

JPH0654687A PRODUCTION OF CARRIER FOR IMMOBILIZING MICROORGANISM 失效
公开(公告)号：JPH0654687A
公开(公告)日：1994-03-01
申请号：JP22927492
申请日：1992-08-05
申请人： FUJI SPINNING CO LTD
发明人： SHINONAGA MASAAKI , KURAHASHI ITSUO , KAWAMURA YOSHIHIDE
IPC分类号： C08B37/08 , C12N11/10
摘要： PURPOSE:To obtain a carrier for immobilizing a microorganism having excellent strength in spite of a large pore diameter and a large pare volume in the interior thereof. CONSTITUTION:The objective carrier for immobilizing a microorganism is obtained by dissolvinig low-molecular weight chitosan in an acidic aqueous solution, dropping the resultant solution containing the chitosan dissolved therein into a basic solution, treating the formed granular porous chitosan with an acid, then N-acylating the granular porous chitosan and reacting the N-acylated product with glycidyl ether of an aliphatic polyalcohol.

24. 发明专利

JPH0455610B2 失效
公开(公告)号：JPH0455610B2
公开(公告)日：1992-09-03
申请号：JP20300685
申请日：1985-09-12
申请人： FUJI SPINNING CO LTD
发明人： YABE HIROAKI , KAWAMURA YOSHIHIDE , KINOSHITA MORISHIGE
IPC分类号： C08B37/08 , A61K8/72 , A61K8/73 , B01J20/281 , C07K17/10 , C08J3/12 , C12N11/02 , G01N30/88

25. 发明专利

JPH0416157B2 失效
公开(公告)号：JPH0416157B2
公开(公告)日：1992-03-23
申请号：JP27392887
申请日：1987-10-29
申请人： FUJI SPINNING CO LTD
发明人： KAWAMURA YOSHIHIDE
IPC分类号： C12N11/10

26. 发明专利

JPH02225539A PRODUCTION OF POROUS CHITOSAN MOLDING 失效
公开(公告)号：JPH02225539A
公开(公告)日：1990-09-07
申请号：JP4578189
申请日：1989-02-27
申请人： FUJI SPINNING CO LTD
发明人： SHINONAGA MASAAKI , KAWAMURA YOSHIHIDE
IPC分类号： C08J9/28 , B01J20/24 , C08L5/00
摘要： PURPOSE:To obtain a chitosan molding having pores of a diameter substantially as large as that of pores in the inside even on the surface or its proximity by treating a porous chitosan molding obtained by depositing the chitosan by coagulating an acidic aqueous low-MW chitosan solution in a basic solution with an acid solution. CONSTITUTION:An acidic aqueous solution of a low-MW chitosan is coagulated in a basic aqueous solution to obtain a porous chitosan molding as a deposit. This molding is treated with an acid solution to obtain the title molding. As the chitosan, a low-MW chitosan of an average MW of 10000-230000 is used, and this chitosan is dissolved in an aqueous solution of acetic acid, dichloroacetic acid, formic acid or a mixture thereof to obtain an acidic aqueous chitosan solution. To obtain a porous chitosan molding by regenerating the chitosan from this solution, the solution is dropped in portions of a given quantity into the basic aqueous solution under pressure through a nozzle of a hole diameter of for example, 0.1-0.25mm to form porous chitosan particles.

27. 发明专利

JPH0116420B2 失效
公开(公告)号：JPH0116420B2
公开(公告)日：1989-03-24
申请号：JP16119284
申请日：1984-07-31
申请人： FUJI SPINNING CO LTD
发明人： KAWAMURA YOSHIHIDE , YABE HIROAKI , KURAHASHI ITSUO , SEO HIROSHI , NAKAJIMA SABURO
IPC分类号： B01J20/281 , B01J20/22 , C08J9/28 , G01N30/88

28. 发明专利

JPS6327501A CHITIN OR CHITOSAN MINUTE PARTICLE CONTAINING ULTRAFINE POWDER OF INORGANIC SUBSTANCE 失效
公开(公告)号：JPS6327501A
公开(公告)日：1988-02-05
申请号：JP17262186
申请日：1986-07-22
申请人： FUJI SPINNING CO LTD
发明人： KAWAMURA YOSHIHIDE , YABE HIROAKI , HIROSE EIJI
IPC分类号： A61K8/72 , A61K8/00 , A61K8/18 , A61K8/29 , A61K8/73 , A61Q1/02 , A61Q17/04 , C08B37/08
摘要： PURPOSE:Ultrafine particles suitable as a base for makeup, having extremely high ultraviolet light scattering ability, improved spread and extension on the skin, obtained by dispersing ultrafine powder titanium oxide, an inorganic substance, into chitin or chitosan minute particles. CONSTITUTION:A dispersed suspension of chiton or chitosan minute particles (having preferably 1-100mum particle diameter) and ultrafine powder titanium oxide (having preferably 0.01-1.0mum particle diameter) is spray-dried in a high- temperature atmosphere usually at 100-180 deg.C to give the aimed ultrafine particles. The dispersed suspension, for example, can be obtained by dripping an acidic solution of low-molecular-weight chitosan (having preferably 10,000-230,000 average molecular weight) to a basic solution to give chitosan particles or by treating the chitosan particles with acetic anhydride to give regenerated particles and adding ultrafine powder titanium oxide to the chitosan particles of the regenerated particles.

29. 发明专利

JPS6321561A CARRIER FOR IMMOBILIZING BIOLOGICALLY ACTIVE SUBSTANCE AND ITS MANUFACTURE 失效
公开(公告)号：JPS6321561A
公开(公告)日：1988-01-29
申请号：JP16539486
申请日：1986-07-14
申请人： FUJI SPINNING CO LTD
发明人： KAWAMURA YOSHIHIDE , YABE HIROAKI , HIROSE EIJI
IPC分类号： A61K47/36 , A61K47/00 , A61K47/34 , G01N33/548 , G01N33/551
摘要： PURPOSE:To prevent the lowering of activity in a biologically active substance with a chitosan film attached onto a base material, by forming the chitosan film on the base material after the base material is treated with a silane coupling agent. CONSTITUTION:The surface of a base material undergoes a silane coupling treatment and a low molecular chitosan acid aqueous solution attached onto the surface of the base material is treated in a basic solution. Thereafter, a contact crosslinking reaction is performed by organic diisocyanate. As a result, a chitosan film is coupled firmly by making -NCO group of the organic isocyanate bond to not only amino group of chitosan but also a silane coupling agent.

30. 发明专利

JPS6238246A PREPARATION OF CHITOSAN ANION EXCHANGE BODY 失效
公开(公告)号：JPS6238246A
公开(公告)日：1987-02-19
申请号：JP17526585
申请日：1985-08-09
申请人： FUJI SPINNING CO LTD
发明人： KAWAMURA YOSHIHIDE , YABE HIROAKI , KURAHASHI ITSUO , HIROSE EIJI
IPC分类号： B01J20/281 , B01J41/16 , C08B37/08 , C12N11/10 , G01N30/88
摘要： PURPOSE:To enhance ion exchange capacity and to enhance strength, by catalytically crosslinking granular porous chitosan with an org. diisocyanate compound in a polar solvent and subsequently reacting the crosslinked product with org. halide in a state contacted with a basic solution. CONSTITUTION:Low M.W. chitosan is dissolved in an acidic aqueous solution and the resulting solution is dripped in a basic solution to form granular porous chitosan. Subsequently, said granular porous chitosan is catalytically crosslinked to an org. diisocyanate compound in a polar solvent and subsequently contacted with a basic solution. The crosslinked product is further reacted with org. halide having a substituted or unsubstituted amino group or a substituted quaternary ammonium salt to prepare an anion exchanger. As chitosan, low M.W. chitosan with average M.W. of 10,000-230,000 is suitable. As the polar solvent, alcohols such as methanol and ketones such as acetone are designated.

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