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1. 发明专利

JP2002300875A METHOD FOR PRODUCING ALKALINE EARTH METAL-TREATED IMMOBILIZED ENZYME 失效
公开(公告)号：JP2002300875A
公开(公告)日：2002-10-15
申请号：JP2001105696
申请日：2001-04-04
申请人： FUJI SPINNING CO LTD
发明人： KAWAMURA YOSHIHIDE , ISHIKAWA EIKO , YABE HIROAKI
IPC分类号： C12N11/10 , C12N11/08
摘要： PROBLEM TO BE SOLVED: To provide a method for producing an immobilized enzyme having an excellent enzyme activity in a polar solvent, especially a water-containing polar solvent and capable of exhibiting a stable activity even when repetitively used. SOLUTION: This method for producing an alkaline earth metal-treated immobilized enzyme comprises moderating an acidic atmosphere near an enzyme causing the deterioration of activity expression of the enzyme by coordinating an alkaline earth metal to a carboxylic acid produced by reacting maleic anhydride with amino group of chitosan or water, namely, reacting 1 pt.wt. of a dried regenerated granular porous chitosan carrier with 0.05-0.60 pt.wt. of a dried copolymer of an alkyl ether of vinyl alcohol and maleic anhydride, then immobilizing the enzyme on the regenerated granular porous chitosan carrier after the treatment with a solution containing a copolymer of an alkyl ether of vinyl alcohol and maleic anhydride with an aqueous solution of the enzyme and subsequently subjecting the resultant immobilized enzyme to dipping treatment in an aqueous solution containing an alkaline earth metal when the regenerated granular porous chitosan carrier is treated with the solution containing the copolymer of the alkyl ether of vinyl alcohol and the maleic anhydride.

2. 发明专利

JPH09271393A ESTER TRANSFER REACTION 失效
公开(公告)号：JPH09271393A
公开(公告)日：1997-10-21
申请号：JP10457296
申请日：1996-04-02
申请人： FUJI SPINNING CO LTD
发明人： KAWAMURA YOSHIHIDE , SHINONAGA MASAAKI
IPC分类号： C12N11/02 , C12P41/00
摘要： PROBLEM TO BE SOLVED: To obtain an optically active alcohol which is useful as an intermediate for physiologically active substances, liquid crystals and the like by asymmetric ester transferring reaction between a racemic alcohol and an acyl donor, for example, a vinyl fatty acid ester using an immobilized catalyst in the presence of a specific amount of an adsorbent. SOLUTION: Chitosan is dissolved in an aqueous acetic acid, the solution is added dropwise to a coagulation solution to form porous particles with an average particle size of 0.1mm, the particles are crosslinked to prepare a support for immobilizing a biocatalyst. The immobilizing support is treated with glutaraldehyde, allowed to react with a biocatalyst, for example, lipase to prepare a immobilized biocatalyst. This biocatalyst is used to effect ester transfer reaction of a racemic mixture of an optically active alcohol with an acyl donor of a vinyl fatty acid ester of the formula (n is 0-16) in the presence of an adsorbent insoluble in an organic solvent in an amount of 0.01-0.1-time the weight of the acyl donor whereby the objective optically native alcohol useful as a synthetic intermediate for physiologically active substances such as medicines, agrochemicals and the like or for liquid crystal materials is obtained.

3. 发明专利

JPH08205866A PRODUCTION OF CARRIER FOR ENZYME IMMOBILIZATION 失效
公开(公告)号：JPH08205866A
公开(公告)日：1996-08-13
申请号：JP3917295
申请日：1995-02-03
申请人： FUJI SPINNING CO LTD
发明人： SHINONAGA MASAAKI , KAWAMURA YOSHIHIDE
IPC分类号： C12N11/10 , C08B37/08
摘要： PURPOSE: To obtain a carrier high in enzyme immobilization level in immobilizing an enzyme using not only a purified enzyme solution but also unpurified one, and excellent in manifestation strength and manifestation rate. CONSTITUTION: Granular porous chitosan produced by dripping an acidic aqueous solution of low-molecular weight chitosan into a basic solution to effect coagulation and regeneration of chitosan is reacted with an aliphatic polyalcohol diglycidyl ether to produce crosslinked granular porous chitosan, which is then reacted with phenyl glycidyl ether suspended in water in the presence of a surfactant, to obtain the objective carrier for enzyme immobilization. Alternatively, phenyl glycidyl ether is reacted in a polar solvent with granular porous chitosan formed by dripping an acidic aqueous solution of low-molecular weight chitosan into a basic solution to effect coagulation and regeneration of chitosan followed by reaction of the resultant granular porous chitosan with an aliphatic polyalcohol diglycidyl ether to obtain the objective carrier.

4. 发明专利

JPH0638755A REGENERATION OF SUPPORT FOR MICROORGANISM IMMOBILIZATION 失效
公开(公告)号：JPH0638755A
公开(公告)日：1994-02-15
申请号：JP21663692
申请日：1992-07-23
申请人： FUJI SPINNING CO LTD
发明人： KAWAMURA YOSHIHIDE , SHINONAGA MASAAKI
IPC分类号： C12N11/10
摘要： PURPOSE:To restore the support used in immobilization of micro-organisms to the state where it can be readily utilized again, by effecting efficient removal of the microorganisms inside the support without any damage to its various properties for immobilization. CONSTITUTION:The microorganisms which have been proliferated inside the support of porous granules of chitosan for immobilization are treated with hot alkali, washed with water, then treated with such an enzyme as to solubilize the constituents of the cell walls of the microorganism whereby the microorganisms inside the support are dissolved and removed.

5. 发明专利

JPH0585158B2 失效
公开(公告)号：JPH0585158B2
公开(公告)日：1993-12-06
申请号：JP11283890
申请日：1990-04-27
申请人： FUJI SPINNING CO LTD
发明人： SHINONAGA MASAAKI , KAWAMURA YOSHIHIDE
IPC分类号： C12N11/10 , C08B37/08

6. 发明专利

JPH0542258B2 失效
公开(公告)号：JPH0542258B2
公开(公告)日：1993-06-28
申请号：JP19162485
申请日：1985-08-30
申请人： FUJI SPINNING CO LTD
发明人： ITO YOSHIO , KAWAMURA YOSHIHIDE , YABE HIROAKI
IPC分类号： C12M3/00 , C12N5/00 , C12N5/07 , C12N5/071 , C12N11/10 , C12N5/06

7. 发明专利

JPH0515728B2 失效
公开(公告)号：JPH0515728B2
公开(公告)日：1993-03-02
申请号：JP24089685
申请日：1985-10-28
申请人： FUJI SPINNING CO LTD
发明人： KAWAMURA YOSHIHIDE , KINOSHITA MORISHIGE , ITO YOSHIO , HIROSE EIJI , ITOYAMA MITSUNORI , UEDA KYOTERU
IPC分类号： C08B37/08 , A61K8/72 , A61K8/73 , C08J3/12

8. 发明专利

JPH0411888A CARRIER FOR IMMOBILIZING ACETIC ACID BACTERIUM AND PRODUCTION THEREOF 失效
公开(公告)号：JPH0411888A
公开(公告)日：1992-01-16
申请号：JP11283890
申请日：1990-04-27
申请人： FUJI SPINNING CO LTD
发明人： SHINONAGA MASAAKI , KAWAMURA YOSHIHIDE
IPC分类号： C12N11/10 , C08B37/08
摘要： PURPOSE:To obtain the subject carrier capable of enhancing compressive elastic modulus and increasing adsorption of acetic acid bacteria by forming an acidic aqueous solution of low-molecular weight chitosan in a basic aqueous solution, then treating the resultant formed chitosan with an acid and subsequently reacting the treated chitosan with phenyl isocyanate. CONSTITUTION:An acidic aqueous solution of low-molecular weight chitosan is coagulated and deposited in a basic aqueous solution to provide a cellular chitosan granular substance, which is then treated with an acid solution and further reacted with phenyl isocyanate to afford the objective carrier having 4-45 phenyl groups introduced thereinto based on 100 structural units of pyranose rings constructing the chitosan of the cellular granular substance and 3-10mum pore diameter of the aforementioned cellular granular substance.

9. 发明专利

JPH026733B2 失效
公开(公告)号：JPH026733B2
公开(公告)日：1990-02-13
申请号：JP30970086
申请日：1986-12-25
申请人： FUJI SPINNING CO LTD
发明人： YABE HIROAKI , KAWAMURA YOSHIHIDE , KURAHASHI ITSUO
IPC分类号： C08B37/08 , A61K8/00 , A61K8/18 , A61K8/72 , A61K8/73 , A61Q1/02 , A61Q17/04

10. 发明专利

JPS6354284B2 失效
公开(公告)号：JPS6354284B2
公开(公告)日：1988-10-27
申请号：JP16119184
申请日：1984-07-31
申请人： FUJI SPINNING CO LTD
发明人： KURAHASHI ITSUO , YABE HIROAKI , KAWAMURA YOSHIHIDE , SEO HIROSHI
IPC分类号： C08B37/08

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