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11. 发明专利

JP2004271430A Manufacturing method for micro fluid element 有权
公开(公告)号：JP2004271430A
公开(公告)日：2004-09-30
申请号：JP2003064912
申请日：2003-03-11
申请人： Kawamura Inst Of Chem Res , 財団法人川村理化学研究所
发明人： TAKADA TETSUO , ANAZAWA TAKANORI , TERAMAE ATSUSHI
IPC分类号： G01N37/00 , B81C99/00 , G01N30/52 , G01N30/56 , G01N30/60 , G03F7/20 , B81C5/00
CPC分类号： G01N30/6052 , G01N30/6095 , G01N2030/525 , G01N2030/562 , Y10T137/2224
摘要： PROBLEM TO BE SOLVED: To form a porous layer capable of immobilizing optimally a large amount of, for example, a protein such as an enzyme and an antigen, a catalyst or the like, with a uniform thickness, on a surface of a fine flow passage, without blocking the flow passage, in the surface inside the flow passage of a micro fluid element. SOLUTION: The porous layer having a large number of pores on its surface is formed on a support body, an active energy radiation-curable composition is applied on the porous layer to fill the pores therewith, a portion other than a portion to be formed into the flow passage is cured by exposure and development of the radiation-curable composition to form a recess with the porous layer exposed in a bottom face, a member serving as a lid is fixed thereafter to the recess to form the flow passage, the porous layer is easily formed thereby with the uniform thickness on the surface of the flow passage. COPYRIGHT: (C)2004,JPO&NCIPI

12. 发明专利

JP2004194652A Microfluidic element having soluble material-adhering passage and method for using same 审中-公开
标题翻译：具有可溶性材料粘合剂的微流体元件及其使用方法
公开(公告)号：JP2004194652A
公开(公告)日：2004-07-15
申请号：JP2003393739
申请日：2003-11-25
申请人： Dainippon Ink & Chem Inc , Kawamura Inst Of Chem Res , 大日本インキ化学工業株式会社 , 財団法人川村理化学研究所
发明人： TORII MIHO , ANAZAWA TAKANORI , TAKADA TETSUO , TERAMAE ATSUSHI
IPC分类号： G01N33/53 , B01J19/00 , B81B1/00 , C12M1/00 , C12N15/09 , G01N33/566 , G01N35/08 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide a method for accurately preparing a minute amount of a reaction solution and a treatment solution, and introducing the same into a microfluidic element, especially without complicating an operation and a structure, a method for adding another ingredient in the middle of fluid passages, and the microfluidic element for the same.
SOLUTION: The microfluidic element has capillary flow passages on the inside of a structural material. The flow passages have soluble material-adhered flow passages in which a (bio)chemical material soluble in the fluid adheres to at least a part of the fluid passages.
COPYRIGHT: (C)2004,JPO&NCIPI
摘要翻译：要解决的问题：为了提供精确地制备微量反应溶液和处理溶液并将其引入微流体元件的方法，特别是不使操作和结构复杂化的方法，添加另一种方法流体通道中间的成分，以及用于其的微流体元件。
解决方案：微流体元件在结构材料的内部具有毛细管流动通道。流动通道具有可溶解的材料粘附的流动通道，其中可溶于流体的（生物）化学材料粘附到至少一部分流体通道。版权所有（C）2004，JPO＆NCIPI

13. 发明专利

JP2004191357A Analysis method of polynucleotide 审中-公开
公开(公告)号：JP2004191357A
公开(公告)日：2004-07-08
申请号：JP2003343140
申请日：2003-10-01
申请人： Kawamura Inst Of Chem Res , 財団法人川村理化学研究所
发明人： ANAZAWA TAKANORI , TERAMAE ATSUSHI , TAKADA TETSUO
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N1/00 , G01N1/28 , G01N21/78 , G01N33/566 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide an analysis method which can rapidly and reliably determine the presence of a target base sequence in a polynucleotide to be examined which is present in a solution sample, the difference from the target base sequence and the like, by easy operations. SOLUTION: An analysis method of polynucleotides which determines at least one selected from the presence in a polynucleotide to be examined of a polynucleotide (A) having a target base sequence, the presence therein of a polynucleotide (B) having no target base sequence and the difference from the target base sequence in the polynucleotide (B), the method comprising the detection step of flowing a test solution containing the polynucleotide to be examined, a single-strand probe compound having a base sequence complementary to the target base sequence and a fluorescent intercalator through a capillary detection flow path having a controlled temperature and a temperature distribution in the flowing detection to measure the fluorescent intensity in an arbitrary position of the flow path, wherein the determination is conducted at the fluorescent intensity. COPYRIGHT: (C)2004,JPO&NCIPI

14. 发明专利

JP2004144568A Liquid chromatography device and sample introduction method 有权
公开(公告)号：JP2004144568A
公开(公告)日：2004-05-20
申请号：JP2002308680
申请日：2002-10-23
申请人： Kawamura Inst Of Chem Res , 財団法人川村理化学研究所
发明人： ANAZAWA TAKANORI , TAKADA TETSUO , TERAMAE ATSUSHI
IPC分类号： G01N31/20 , G01N30/10 , G01N30/16 , G01N30/26 , G01N30/32 , G01N30/60 , G01N37/00
CPC分类号： G01N30/16 , G01N30/10 , G01N30/6095
摘要： PROBLEM TO BE SOLVED: To provide a micro-liquid chromatography device equipped with a micro-column, capable of introducing a very small quantity of sample into the column and forming a sample adsorption band having a narrow band width, and a sample introduction method into the liquid chromatography device.
SOLUTION: This micro-liquid chromatography device 200 has inside a member, a capillary-shaped upstream side passage 3, a liquid injection part 13 positioned at the upstream end of the upstream side passage, an adsorption column 5 having one end connected to the terminal of the upstream side passage, and a downstream side discharge port 14 for discharging liquid passing through the adsorption column. The device is characterized as follows: a branch part 4 is provided on the upstream side passage; a branch passage 7 is provided from the branch part; and the branch passage is connected to the outside of the device through a gas-permeable and liquid-impermeable gas-liquid separator 8.
COPYRIGHT: (C)2004,JPO

15. 发明专利

JP2004049223A Method for detecting of polynucleotide, fluid device and apparatus for detecting of polynucleotide 审中-公开
公开(公告)号：JP2004049223A
公开(公告)日：2004-02-19
申请号：JP2003149070
申请日：2003-05-27
申请人： Dainippon Ink & Chem Inc , Kawamura Inst Of Chem Res , 大日本インキ化学工業株式会社 , 財団法人川村理化学研究所
发明人： NISHIE HARUO , ANAZAWA TAKANORI , TAKADA TETSUO , TERAMAE ATSUSHI
IPC分类号： G01N33/53 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , G01N33/566 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting polynucleotide, quickly identifying the presence or absence of a polynucleotide with a specific base sequence in a specimen containing a number of polynucleotides with different base sequences.
SOLUTION: The method for detecting the polynucleotide with the specific base sequence detects the presence or absence of the polynucleotide with the specific base sequence comprises measuring concentration of the polynucleotide in a sample solution (Ni) before charging into a flow path having a diameter of 3-200 μm and having a path wall with affinity to the objective polynucleotide of the detection with the specific base sequence, measuring concentration of the polynucleotide in a sample solution (No) discharged from the flow path, and comparing the concentration (Ni) and (No).
COPYRIGHT: (C)2004,JPO

16. 发明专利

JP2010018775A Organic and inorganic composite material dispersion and method for its preparation 有权
标题翻译：有机和无机复合材料分散体及其制备方法
公开(公告)号：JP2010018775A
公开(公告)日：2010-01-28
申请号：JP2009049127
申请日：2009-03-03
申请人： Kawamura Inst Of Chem Res , 財団法人川村理化学研究所
发明人： TAKADA TETSUO , HARAGUCHI KAZUTOSHI
IPC分类号： C08L33/14 , B32B27/30 , C08F20/28 , C08K3/34 , C08K7/00 , C09D1/00 , C09D133/14 , C09K3/18 , C12M1/00
摘要： PROBLEM TO BE SOLVED: To provide an aqueous dispersion of an organic and inorganic composite material with an organic and inorganic composite material particles having a clay mineral and a macromolecular polymer forming a three-dimensional network, capable of easily controlling the composite structure and the particle size thereof and having the excellent coating film forming ability and bonding property with respect to a substrate stably dispersed in water, and to provide a preparation of the aqueous solution simply in a short time over a wide range of a clay mineral content.
SOLUTION: The organic and inorganic composite dispersion comprises (X) organic and inorganic composite material particles prepared by multiplexing (P) a polymer from (a) a monomer represented by formula (1): CH
2 =CR
1 -CO(OR
2 )
n OR
3 , and (B) one or more of inorganic materials selected from the group consisting of a water-swellable clay mineral and a silica dispersed in (C) an aqueous solution.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：为了提供有机和无机复合材料的水性分散体，其具有有机和无机复合材料颗粒，其具有形成三维网络的粘土矿物和大分子聚合物，能够容易地控制复合结构并且具有优异的涂膜形成能力和相对于稳定分散于水中的基材的粘结性能，并且在宽范围的粘土矿物质含量下简单地在短时间内提供水溶液的制备。有机和无机复合分散体包含（X）通过使（A）由式（1）表示的单体的聚合物（P）复合（P）制备的（X）有机和无机复合材料颗粒：CH 2 => >（B）一个或多个选自水溶胀性粘土矿物和分散在（C）水溶液中的二氧化硅）的无机材料。版权所有（C）2010，JPO＆INPIT

17. 发明专利

JP2005194951A Microfluid device pump and method of transferring fluid 审中-公开
标题翻译：微流体装置泵和输送流体的方法
公开(公告)号：JP2005194951A
公开(公告)日：2005-07-21
申请号：JP2004002920
申请日：2004-01-08
申请人： Kawamura Inst Of Chem Res , 財団法人川村理化学研究所
发明人： ANAZAWA TAKANORI , TAKADA TETSUO
IPC分类号： F04B43/12
摘要： PROBLEM TO BE SOLVED: To provide a method of transferring a fluid which enables the use of a pump mechanism of small size and simple structure and which dispenses with a complicated control and to provide, moreover, a microfluid device pump which can be manufactured easily with the simple structure and having the pump mechanism which has good quantitativeness and which can be applied to gas transfer as well. SOLUTION: The method of transferring the fluid includes (i) a step of circulating the fluid by moving the pressed part in the direction of a valve while deforming a capillary tube-like flow path cross section by pressing the pressed part and opening the valve by the pressurization of the fluid by using the microfluid device pump having the valve opened by the pressurization of the fluid on the way of the capillary tube-like flow path and the pressed part which deforms the flow path by the pressing, or (ii) a step of transferring the fluid by moving the pressed part in the reverse direction to the valve while deforming the pressed part by pressing from the exterior and opening the valve by the pressure reduction of the fluid by using the microfluid device pump having the valve opened by the pressurization of the fluid on the way of the capillary tube-like flow path and the pressed part which deforms the flow path by the pressing. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供一种转移流体的方法，该方法能够使用尺寸小且结构简单的泵机构，其不需要复杂的控制，并且还提供一种微流体装置泵，其可以是制造容易，结构简单，泵机构具有良好的定量性，可应用于气体输送。解决方案：传送流体的方法包括：（i）通过沿着阀的方向移动受压部分，同时使毛细管状流路横截面通过按压按压部分和打开而变形而使流体循环的步骤通过使用具有通过在毛细管状流动路径上的流体的加压而打开的阀的微流体装置泵和通过按压使流路变形的被挤压部分来加压流体的阀，或 ii）通过使用具有阀的微流体装置泵，通过从外部按压使压迫部分变形并通过流体的压力降低来使阀打开而使按压部件沿与阀的相反方向移动的步骤，通过在毛细管状流路的路径上的流体的加压和通过按压使流路变形的被挤压部而开启。版权所有（C）2005，JPO＆NCIPI

18. 发明专利

JP2005091135A Member for liquid chromatography, and processing method by liquid chromatography 审中-公开
标题翻译：液相色谱仪及液相色谱法处理方法
公开(公告)号：JP2005091135A
公开(公告)日：2005-04-07
申请号：JP2003324439
申请日：2003-09-17
申请人： Kawamura Inst Of Chem Res , 財団法人川村理化学研究所
发明人： TAKADA TETSUO , ANAZAWA TAKANORI , TERAMAE ATSUSHI
IPC分类号： G01N31/20 , B01J20/281 , G01N30/02 , G01N30/18 , G01N30/26 , G01N30/32 , G01N30/54 , G01N30/56 , G01N30/60 , G01N30/74 , G01N30/88 , G01N37/00 , G01N30/48
摘要： PROBLEM TO BE SOLVED: To provide a member for liquid chromatography allowing quick analysis even when a developing agent flows at low pressure, and a separation method by the liquid chromatography using the same.
SOLUTION: A bulking agent is not required to be filled, the developing agent is allowed to be fed at the low pressure, and the accurate and quick analysis is allowed, by using the member for the liquid chromatography having a separation column in which at least one portion on an inner face is a porous layer, preferably the member for the liquid chromatography having the separation column fixed, on the porous layer, with a probe having affinity with a specified substance.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供用于液相色谱的成分，即使显影剂在低压下流动也能进行快速分析，以及通过使用其的液相色谱法进行分离。解决方案：不需要填充填充剂，显影剂可以在低压下进料，通过使用具有分离塔的液相色谱成分，可以进行准确快速的分析其中内表面上的至少一部分是具有多孔层的多孔层，优选地是具有分离柱的液相色谱用部件固定在多孔层上，探针与特定物质具有亲和力。版权所有（C）2005，JPO＆NCIPI

19. 发明专利

JP2005024445A Member for affinity electrophoresis and method for processing affinity electrophoresis 审中-公开
标题翻译：亲属电泳会员及处理亲和电泳的方法
公开(公告)号：JP2005024445A
公开(公告)日：2005-01-27
申请号：JP2003191884
申请日：2003-07-04
申请人： Kawamura Inst Of Chem Res , 財団法人川村理化学研究所
发明人： TERAMAE ATSUSHI , ANAZAWA TAKANORI , TAKADA TETSUO
IPC分类号： G01N33/53 , G01N27/447 , G01N33/566
摘要： PROBLEM TO BE SOLVED: To provide a member for affinity electrophoresis which rapidly and accurately separates and detects an analyzed object and a method for rapidly and accurately processing electrophoresis. SOLUTION: The member for the affinity electrophoresis has a minute flow path and a porous layer on which a probe is fixed and has an affinity to the analyzed object in a part of an inner wall face of the flow path. An average distance between the porous layer and the facing inner wall has a range within 1-50 μm. In the method for processing the electrophoresis, the material substantially having the affinity to the probe and contained in a solution having viscosity of 0.2-20 (mPs), is separated and detected by the electrophoresis. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供快速且准确地分离和检测分析对象的亲和电泳成员和快速准确地加工电泳的方法。解决方案：用于亲和电泳的构件具有微小的流路和多孔层，探针固定在其上，并且在流路的内壁面的一部分中与分析对象具有亲和性。多孔层与面对的内壁之间的平均距离在1-50μm范围内。在电泳处理方法中，通过电泳分离并检测基本上对探针具有亲和力并且含有粘度为0.2-20（mPs）的溶液）的材料。版权所有（C）2005，JPO＆NCIPI

20. 发明专利

JP2004226396A Polynucleotide analyzing method 审中-公开
公开(公告)号：JP2004226396A
公开(公告)日：2004-08-12
申请号：JP2003194275
申请日：2003-07-09
申请人： Kawamura Inst Of Chem Res , 財団法人川村理化学研究所
发明人： ANAZAWA TAKANORI , TERAMAE ATSUSHI , TAKADA TETSUO
IPC分类号： G01N33/53 , C12Q1/68 , G01N21/77 , G01N21/78 , G01N33/566 , G01N33/58
摘要： PROBLEM TO BE SOLVED: To provide an analyzing method for rapidly determining the presence or absence of a target base sequence in polynucleotide to be inspected in a sample solution, the difference with the target base sequence or the like with good reliability by simple operation. SOLUTION: The analyzing method for polynucleotide is constituted so as to determine at least one of the presence or absence of polynucleotide (A) having a target base sequence, the presence or absence of polynucleotide (B) having no target base sequence and the difference from the target base sequence in the polynucleotide (B), in a polynucleotide to be inspected. This analyzing method has a process for letting a specimen solution which contains polynucleotide to be inspected, a single stranded probe compound having a base sequence substantially complementary with respect to the target base sequence and a fluorescent intercalator, flow through a capillary detection flow channel regulated in temperature and measuring the intensity of fluorescence in the flow channel, and the determination mentioned above is performed based on the measured value of the intensity of fluorescence. COPYRIGHT: (C)2004,JPO&NCIPI

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