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1. 发明公开

EP0151492A3 Heterologous system for the detection of chemically-labeled DNA and other biological materials providing a receptor or target moiety thereon 失效
标题翻译：用于检测化学标记的DNA和其他生物材料的异位系统，提供受体或目标物质
公开(公告)号：EP0151492A3
公开(公告)日：1988-11-09
申请号：EP85101353
申请日：1985-02-08
申请人： ENZO BIOCHEM, INC.
发明人： Rabbani, Elazar
IPC分类号： G01N33/535 , C12Q01/68 , G01N33/53
CPC分类号： C12Q1/6813 , G01N33/535 , Y10S435/803 , Y10S435/81 , Y10S436/808 , Y10S436/824 , Y10S436/827
摘要： The detection and/or identification of materials of interest, such as biological materials, e.g. chemically labeled DNA, which provide a receptor thereon, is improved by employing a heterologous detection system by employing more than one binding reactions, such as reactions involving the affinity of lectin to a glycoprotein or sugar group and the affinity between biotin and avidin or streptavidin, avidin itself being a glycoprotein. Particularly useful in the heterologous detection system are biotinylated lectins, such as biotinylated Concanayalin A, biotinylated dextran, lectingly- coprotein complexes, such as lectin-avidin and lectinenzyme complexes and streptavidin-biotinylated enzyme complexes. By way of example, the detection of single-stranded glycosy- toted DNA hybridized to complementary single-stranded DNA is detected by bringing the hybridized double-stranded DNA into contact with a lectin, such as Concanavalin A, for attachment to the glycosyl group of the glycosylated DNA, followed by contact with a biotinylated dextran, a polysaccharide, and then by contact with a lectin-enzyme complex, such as Concanavalin A-horseradish peroxidase or acid phosphatase. Also, there would be employed an enzyme complex comprising streptavidin-biotinylated horseradish peroxidase. The lectin-enzyme complex would attach itself to the sugar or saccharide of the biotinylated dextran which is also attached to the lectin which is fixed to the glycosyl groups of the hybridized DNA. The streptavidin-biotinylated enzyme complex would attach itself to the biotin moieties of the biotinylated dextran which, as indicated, is linked to the lectin attached to the glycosyl groups of the target double-stranded DNA.

2. 发明公开

EP0135108A3 Nucleotide hybridization assay for protozoan parasites 失效
标题翻译：用于原生土壤的核酸杂交测定
公开(公告)号：EP0135108A3
公开(公告)日：1988-07-13
申请号：EP84109346
申请日：1984-08-07
申请人： ROCKEFELLER UNIVERSITY
发明人： Lizardi, Paul , Nogueira, Nadia
IPC分类号： C12Q01/68 , C12N15/00 , C12P19/34 , C07H21/00
CPC分类号： C07K14/445 , C12Q1/6893 , Y02A50/58
摘要： The present invention provides a method for detection of protozoan parasites in blood or other specimen from their mammalian hosts. The method comprises nucleic acid hybridization of repetitive nuclear DNA fragments of the parasites. Hybridization probes have been prepared for this purpose by cloning the repetitive nuclear elements that are species-specific in appropriate vectors. The sensitivity of these probes has been increased by further sub-cloning to make them capable of cascade hybridization. The assay is highly specific and sensitive for detection of disease-causing protozoan parasites of the commonly occurring Trypanosomatidae of the genus Leishmania and genus Trypanosoma, as well as for malaria-causing protozoan and other parasitic microorganism of mammals.

3. 发明公开

EP0159719A3 Hybridization method for the detection of genetic materials 失效
标题翻译：用于检测遗传材料的杂交方法
公开(公告)号：EP0159719A3
公开(公告)日：1988-03-30
申请号：EP85105130
申请日：1985-04-26
申请人： ENZO BIOCHEM, INC.
发明人： Rabbani, Elazar , Engelhardt, Dean L.
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6813 , C12Q1/6816 , C12Q1/682 , C12Q1/6827 , C12Q1/683 , C12Q1/6832 , Y10S435/803 , C12Q2565/113 , C12Q2563/179 , C12Q2563/149 , C12Q2565/519
摘要： This invention provides methods for the detection of a target genetic material having a desired base sequence or gene. Also disclosed are methods for the detection of mutations. Also provided are components for use in such methods. The methods are based upon techniques which utilize two labeled single stranded polynucleotide segments which are complementary to the same or the opposite strands of the target genetic material. The methods of the invention result in the formation of a double hybrid and/or a multihybrid.

4. 发明公开

EP0128018A3 Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe 失效
标题翻译：分子遗传探针及其形成方法，使用分子遗传学探针的测定技术和试剂盒
公开(公告)号：EP0128018A3
公开(公告)日：1988-02-10
申请号：EP84303671
申请日：1984-05-31
申请人： Orgenics Ltd.
发明人： Herzberg, Max
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6804 , C12Q1/6816
摘要： A method of assaying for the presence or quantity of a suspect nucleic acid in a suspect sample using a modified genetic probe in combination with an imaging procedure. The probe is modified by addition and provides an easy and very effective technique of modifying a genetic probe preparatory to its use in a kit. The genetic probe is then introduced into contact with DNA or RNAofthesuspectsample and permitted to hybridize therewith. A kit for performing an assay using the modified probe is also disclosed.

5. 发明公开

EP0123300A3 Complexing of biologically active or functional compounds and methods of preparing and utilizing same 失效
标题翻译：生物活性或功能化合物的复合物及其制备和利用方法
公开(公告)号：EP0123300A3
公开(公告)日：1988-01-20
申请号：EP84104488
申请日：1984-04-19
申请人： ENZO BIOCHEM, INC.
发明人： Johnston, Kenneth H.
IPC分类号： G01N33/58 , C12Q01/68 , G01N33/74
CPC分类号： G01N33/74 , C12Q1/6816 , G01N33/531 , G01N33/535 , G01N33/544 , G01N33/551 , C12Q2563/131 , C12Q2563/125
摘要： Complexes containing a biologically active or functional compound or compounds are prepared by attaching or fixing a first compound, which may be a biologically active or functional compound, to a solid substrate under conditions such that the active site of said first compound is protected, thereupon derivatizing or combining said first compound with a derivatizing or modifying compound while said first compound is attached to said solid substrate. The resulting complex or complexed first compound is then released from attachment to said solid substrate to yield the complex, said complex comprising said derivatizing compound attached to said first compound, said first compound, upon release from the solid substrate having available its functional or biologically active site. For example, alkaline phosphatase is attached to a solid substrate, such as dextran beads, preferably through a ligand, such as a competitive inhibitor for alkaline phosphatase, e.g. para-aminobenzyl-phosphonic acid (p-ABPA). The dextran beads would be first treated with (p-ABPA) and the alkaline phosphatase brought into contact with the thus-treated dextran beads. The competitive inhibitor p-ABPA serves as a ligand for attachment of the alkaline phosphatase to the dextran beads while at the same time occupying and protecting the active site of the alkaline phosphatase. Thereupon, the thus-attached alkaline phosphatase is derivatized or modified, such as by biotinylation. Upon biotinylation of the alkaline phosphatase, the resulting biotinylated alkaline phosphatase is eluted or removed from attachment to the p-ABPA treated solid substrate or dextran beads to which it was fixed, yielding a biotinylated alkaline phosphatase with the active or functional site of the alkaline phosphatase intact. The complex, while still attached to the dextran beads, could be further treated with a second compound or component which might also, if desired, provide an active or functional site, such as by contact with avidin, which would bind to the modifying or derivatizing moiety, i.e. biotin, of the biotinylated alkaline phosphatase and the thus-produced avidin-biotinylated alkaline phosphatase complex then eluted. This complex, such as a complex comprising avidin-biotinylated alkaline phosphatase, could be employed as a detector in a detection system for the identification and/or location of another compound, such as a biotinylated compound, e.g. a biotinylated nucleotide or biotinylated DNA.
摘要翻译：含有生物活性或功能性化合物或化合物的复合物可通过将第一化合物（其可以是生物活性或功能性化合物）附着或固定在固体底物上，使得所述第一化合物的活性位点被保护的条件下进行衍生化或将所述第一化合物与衍生化或改性化合物组合，同时所述第一化合物连接到所述固体基质上。然后将所得复合物或络合的第一化合物从附着物释放到所述固体基质上以产生络合物，所述复合物包含与所述第一化合物连接的所述衍生化合物，所述第一化合物在从固体基质释放后具有其功能或生物活性例如，碱性磷酸酶连接到固体底物，例如葡聚糖珠，优选通过配体，例如碱性磷酸酶的竞争性抑制剂，例如对 - 氨基苄基膦酸（p-ABPA），首先用（p-ABPA）处理葡聚糖珠，碱性磷酸酶与如此处理的葡聚糖珠接触。竞争性抑制剂p-ABPA用作碱性磷酸酶附着于葡聚糖珠的配体，同时占据和保护碱性磷酸酶的活性位点。因此，如此附着的碱性磷酸酶被衍生化或修饰，例如通过生物素化。在碱性磷酸酶的生物素化后，所得到的生物素化碱性磷酸酶被洗脱或从与其固定的p-ABPA处理的固体底物或葡聚糖珠的连接中洗脱或除去，产生具有碱性磷酸酶活性或功能位点的生物素化碱性磷酸酶完整。复合物虽然仍然附着在葡聚糖珠上，但可以用第二化合物或组分进一步处理，如果需要，还可以提供活性或功能部位，例如通过与抗生物素蛋白接触，其将结合修饰或衍生化部分，即生物素，生物素化碱性磷酸酶和随后产生的抗生物素蛋白 - 生物素化碱性磷酸酶复合物，然后洗脱。该复合物，例如包含抗生物素蛋白 - 生物素化碱性磷酸酶的复合物，可以用作检测系统中的检测器，用于鉴定和/或定位另一种化合物，例如生物素化化合物，例如生物素化化合物。生物素化的核苷酸或生物素化的DNA。

6. 发明公开

EP0200056A3 Method and kit for detecting polyriboadenosine segments and messenger RNA 失效
标题翻译：检测多核苷酸部分和信使RNA的方法和试剂盒
公开(公告)号：EP0200056A3
公开(公告)日：1988-01-13
申请号：EP86104980
申请日：1986-04-11
申请人： ALLIED CORPORATION , GENETICS INSTITUTE, INC.
发明人： Hull Vary, Calvin Pardee , Diamond, Steven Elliot , Wolfman, Neil M. , Koudelka, Astrid P.
IPC分类号： C12Q01/68 , C12Q01/48 , C12Q01/34
CPC分类号： C12Q1/6816 , C12Q1/48 , C12Q1/68 , C12Q1/6823 , Y10S435/81 , Y10S435/975 , C12Q2527/125 , C12Q2521/525 , C12Q2563/179
摘要： RNA such as messenger RNA is digested to nucleotide phosphates including AMP or ADP. The ATP or a byproduct of the osphorylation, e.g., pyruvate, is detected. Exemplary enzymes d (with appropriate co-reactants and co-factors) are: (1) polynucleotide phosphorylase, pyruvate kinase and luciferase, or 2) phosphodiesterase (or RNase), myokinase, pyruvate kinase and luciferase. The phosphorylation to ATP (e.g., with pyruvate kinase) is preferably coupled with the previous (reversible) enzymatic step.

7. 发明公开

EP0173339A3 Composition and method for the dectection of the presence of a polynucleotide sequence of interest 失效
标题翻译：保护多核苷酸顺序顺序的组合物和方法
公开(公告)号：EP0173339A3
公开(公告)日：1987-12-23
申请号：EP85110910
申请日：1985-08-29
申请人： ENZO BIOCHEM, INC.
发明人： Rabbani, Elazar , Engelhardt, Dean L.
IPC分类号： C12Q01/68 , C12N15/00
CPC分类号： C12Q1/70 , C12Q1/6813 , C12Q1/6832 , C12Q1/689 , Y10T436/143333
摘要： Described is a genetic analyses using the technique of nucleic acid hybridization. The genetic analyses includes, for example, the diagnosis of infections by foreign microbes and the detection of specific genetic traits and abnormalities. Furthermore, a technique for the detection of the presence of a polynucleotide sequence of interest is described.

8. 发明公开

EP0229943A3 Fluorescent stokes shift probes for polynucleotide hybridization assays 失效
标题翻译：荧光检测用于多核苷酸杂交测定的移位探针
公开(公告)号：EP0229943A3
公开(公告)日：1987-11-11
申请号：EP86116652
申请日：1986-12-01
申请人： MOLECULAR BIOSYSTEMS, INC.
发明人： Heller, Michael J. , Jablonski, Edward J.
IPC分类号： C12Q01/68 , C07H21/00
CPC分类号： G01N33/533 , C12Q1/6818 , C12Q1/705
摘要： Fluorescent stokes shift probes for polynucleotide hybridization assays are designed to provide predetermined nucleotide base unit spacings between the donor and acceptor fluorophores. When the probes are hybridized to the target polynucleotide the fluorophores paired for non-radiative energy transfer are separated by 2 to 7 nucleotide base units. The fluorophores are attached to the DNA or RNA probes by linker arms having lengths of 4 to 30 Angstroms. Fluorescein is a preferred donor for use with a Texas Red acceptor.

9. 发明公开

EP0209996A3 Nucleic acid probes comprising N4 -(substituted amino)cytidines 失效
标题翻译：包含N4 - （取代氨基）氨基酸的核酸探针
公开(公告)号：EP0209996A3
公开(公告)日：1987-09-02
申请号：EP86304945
申请日：1986-06-25
申请人： SISKA DIAGNOSTICS,INC.
发明人： Musso, Gary Fred , Ghosh, Soumitra Shankar , Orgel, Leslie Eleazer , Wahl, Geoffrey Myles , Kaiser, Emil Thomas
IPC分类号： C12Q01/68 , C07C143/48 , C07D317/30 , C07D311/82 , C07D319/06 , C07D407/12 , C07D285/12 , C07H21/00 , C07D417/12 , C07F07/18 , C07D495/04 , C07D333/00 , C07D235/00
CPC分类号： C07H21/00 , C07F7/1852 , C07F7/1856 , C12Q1/6816
摘要： Nucleic acid hybridization probes are provided which comprise an N 4 -(substituted amino) cytosine moiety, wherein the substituted amino group comprises a tag moiety, whereby the probe is detected. Methods of preparing probes of the Invention, intermediates used in such methods, and methods of using the probes of the invention in hybridization assays are also provided. Typical tag moieties employed with the probes of the invention are biotinyl, aminothiadiazole and fluorescein derivatives, connected to N 4 -amino groups of modified cytosines of the probe through linker moieties.
摘要翻译：提供了包含N 4 - （取代氨基）胞嘧啶部分的核酸杂交探针，其中取代的氨基包含标签部分，从而检测探针。还提供了制备本发明的探针的方法，在这些方法中使用的中间体以及在杂交测定中使用本发明的探针的方法。与本发明的探针一起使用的典型标签部分是通过接头部分与探针的修饰胞嘧啶的N 4 - 氨基连接的生物素基，氨基噻二唑和荧光素衍生物。

10. 发明公开

EP0223618A3 Automatable process for nucleotide sequencing 失效
标题翻译：核苷酸序列的自动化过程
公开(公告)号：EP0223618A3
公开(公告)日：1987-07-08
申请号：EP86401590
申请日：1986-07-16
申请人： NEW YORK MEDICAL COLLEGE
发明人： Melamede, Robert Jean
IPC分类号： C12Q01/68 , G01N33/48 , G01N21/33
CPC分类号： C12Q1/6858 , C12Q1/6869 , C12Q2535/113 , C12Q2525/101
摘要： This invention relates to a method for determining the nucleotide sequence of DNA and RNA molecules. The method is automatable and avoids the use of radioactive labels and gel electrophoresis. The method is also adaptable for introducing site-specific mutations in DNA and RNA molecules.

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