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1. 发明公开

EP4321620A1 METHOD FOR PRODUCING TAGATOSE BY IMMOBILIZING MULTIPLE ENZYMES BY USING ARTIFICIAL OIL BODY 审中-实审
公开(公告)号：EP4321620A1
公开(公告)日：2024-02-14
申请号：EP22783792.9
申请日：2022-02-11
申请人： TIANJIN YEAHE BIOTECHNOLOGY CO., LTD
发明人： MA, Yanhe , SHI, Ting , HAN, Pingping , LI, Yunjie
IPC分类号： C12N11/18 , C12N11/06 , C12P19/24 , C12P19/18 , C12P19/16 , C12P19/02
摘要： Provided are the immobilization of multiple enzymes on the basis of an artificial oil body and an application thereof in the preparation of tagatose. Specifically, an artificial oil body is used to mix an expressed fusion protein of target protease-oil body protein with an oil body, which then undergoes an ultrasonic treatment; the fusion protein is anchored to the surface of the oil body by means of the specific hydrophobicity of a human protein to form an artificial oil body containing the target protease, so that the purification and immobilization of enzymes can be completed simultaneously. The immobilized multiple enzymes that can be used for tagatose production utilize an artificial oil body as an immobilized enzyme substrate, which significantly improves the stability of the immobilized enzymes, reduces the production cost of the current enzymatic preparation of tagatose, and has a simple preparation process.

2. 发明公开

EP4321619A1 METHOD FOR PRODUCING TAGATOSE FROM BIOMIMETIC SILICON MINERALIZED MICROCAPSULE IMMOBILIZED MULTI-ENZYME 审中-实审
公开(公告)号：EP4321619A1
公开(公告)日：2024-02-14
申请号：EP22783791.1
申请日：2022-02-11
申请人： TIANJIN YEAHE BIOTECHNOLOGY CO., LTD
发明人： MA, Yanhe , SHI, Ting , HAN, Pingping
IPC分类号： C12N11/18 , C12N11/14 , C12N11/089 , C12N11/04 , C12P19/24 , C12P19/18 , C12P19/16 , C12P19/02
摘要： Provided are a biomimetic silicon mineralized microcapsule immobilized multi-enzyme, a preparation method therefor, and a method for producing tagatose by using same. The preparation method comprises the following steps: (1) pre-mixing glucan phosphorylase, phosphoglucomutase, phosphoglucoisomerase, 6-phosphate tagatose 4-position epimerase and 6-phosphate tagatose phosphatase solutions, then adding the mixture to a calcium chloride solution, and then pouring same into a sodium carbonate solution, stirring and separating same to obtain calcium carbonate microspheres containing a multi-enzyme; (2) mixing the calcium carbonate microspheres with a polyethyleneimine solution to obtain polyethyleneimine-calcium carbonate microspheres after separation; (3) mixing the polyethyleneimine-calcium carbonate microspheres with a silicate solution to obtain biomimetic silicon mineralized-calcium carbonate microspheres after separation; and (4) mixing the biomimetic silicon mineralized-calcium carbonate microspheres with ethylenediamine tetraacetic acid for reaction to remove calcium carbonate, and separating same to obtain a biomimetic silicon mineralized microcapsule immobilized multi-enzyme

3. 发明授权

EP3597180B1 DEBRANCHING ENZYME MODIFIED STARCH, THE PREPARING METHOD AND USE THEREOF IN HARD CAPSULE PRODUCTION 有权
公开(公告)号：EP3597180B1
公开(公告)日：2021-05-26
申请号：EP18190455.8
申请日：2018-08-23
发明人： CHANG, Ruei-Jan , CHAO, Hsin-Yi , LEE, Pei-Hsuan , CHEN, Wei-Yu
IPC分类号： C08B30/12 , C08L3/02 , C12P19/16 , A23L29/219 , A61K9/48

4. 发明公开

EP3379945A1 POLYPEPTIDES CAPABLE OF PRODUCING GLUCANS HAVING ALPHA-1,2 BRANCHES AND USE OF THE SAME 审中-公开
公开(公告)号：EP3379945A1
公开(公告)日：2018-10-03
申请号：EP16816788.0
申请日：2016-11-22
申请人： E. I. du Pont de Nemours and Company
发明人： CHENG, Qiong , DICOSIMO, Robert , PRASAD, Jahnavi Chandra , ZHANG, Zhenghong
IPC分类号： A23L33/10 , A61K31/716 , C08B37/00 , C12P19/04 , C12P19/08 , C12P19/16 , C12P19/18 , C12N9/10 , C08L5/00 , C08L5/02 , A23L33/135 , A23L33/21 , A61K8/73
CPC分类号： C07K14/4725 , A23L33/10 , A23L33/135 , A23L33/21 , A23V2002/00 , A23V2200/00 , A61K8/60 , A61K8/64 , A61K8/73 , A61K31/716 , A61K2800/10 , A61Q19/00 , C08B37/0009 , C08L5/00 , C08L5/02 , C11D3/22 , C12N9/1051 , C12P19/04 , C12P19/18 , C12Y204/00 , C12Y204/01 , C12Y204/01005 , C12Y302/01 , A23V2200/32 , A23V2200/3202 , A23V2200/328 , A23V2250/00 , A23V2250/282 , A23V2250/284 , A23V2250/5116
摘要： Disclosed herein are proteins capable of forming glucans having alpha-1,2 linkages/branches, reactions and methods for producing such glucan, compositions comprising such glucan, and various applications thereof.

5. 发明公开

EP3097192A2 PULLULANASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 有权
标题翻译： PULLUONASEVARIANTEN UND POLYNUKLEOTIDE ZUR CODIERUNG DAVON
公开(公告)号：EP3097192A2
公开(公告)日：2016-11-30
申请号：EP15703235.0
申请日：2015-01-21
申请人： Novozymes A/S
发明人： MATSUI, Tomoko , YAMAGISHI, Akihiko
IPC分类号： C12N9/44 , A23L29/30 , C12P19/16
CPC分类号： C12N9/2457 , C12P7/06 , C12P19/02 , C12P19/16 , C12P2203/00 , C12Y302/01041 , Y02E50/17
摘要： The present invention relates to pullulanase variants having increased thermoactivity and comprising substitutions of the parent pullulanase at one or more positions corresponding to positions 393, 143, 150, 243, 244, 345, 346, 368, 370, 373, 381, 382, 385, 387, 402, 429, 430, 431, 432, 456, 486, 492, 610, 624, 631, 632, 665 and 699 of the polypeptide of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The variants are mainly derived from pullulanases from Bacillus deramificans, Bacillus acidopullulyticus or hybrid pullulanases.
摘要翻译：本发明涉及具有增加的热活性的支链淀粉酶变体，并且包括在对应于位置393,143,150,243,244,345,346,368,370,373,381,368,370,373,381,368,370,337,381,382,385的一个或多个位置上的母体支链淀粉酶的取代，387,402,429,430,431,432,456,486,492,626,624,631,632,665和699的多核苷酸。本发明还涉及编码变体的多核苷酸 ; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。变异体主要衍生自来自芽孢杆菌属（Bacillus deramificans），嗜酸芽胞杆菌（Bacillus-islululyticus）或杂合支链淀粉酶的杂合支链淀粉酶的支链淀粉酶。

6. 发明公开

EP2898084A1 PROCESS FOR INCREASING YIELD OF DEXTROSE PRODUCTION PROCESS, BY MEMBRANE TECHNOLOGY 有权
标题翻译：方法增加DEXTROSEPRODUKTIONSVERFAHRENS的效率膜技术
公开(公告)号：EP2898084A1
公开(公告)日：2015-07-29
申请号：EP13770815.2
申请日：2013-09-20
申请人： Cargill, Incorporated
发明人： KETSMAN, Joost , NATALONI, Luigi , SANCHEZ, Samuel , BANDINI, Serena
IPC分类号： C12P19/02 , C12P19/14 , C12P19/16 , C12P19/20
CPC分类号： C12P19/20 , A23L29/35 , C12P19/02 , C12P19/16 , C13K1/08
摘要： The invention relates to a process for increasing the dextrose recovery from a dextrose containing solution. In particular, the invention relates to a process for increasing the dextrose yield of a starch hydrolysis process. The process comprises membrane filtration of a dextrose containing solution and an enzyme treatment of the retentate of the filtration.

7. 发明授权

EP2726623B1 METHODS FOR IMPROVEMENT OF ENZYMATIC HYDROLYSIS OF LIGNOCELLULLOSIC MATERIAL 有权
标题翻译：法木质纤维素材料改善酶水解
公开(公告)号：EP2726623B1
公开(公告)日：2015-06-10
申请号：EP12728446.1
申请日：2012-06-11
申请人： Sekab E-Technology AB
发明人： JÖNSSON, Leif , ALRIKSSON, Björn , SOUDHAM, Venkata, Prabhakar
IPC分类号： C12P7/10 , C12P19/02 , C12P19/04 , C12P19/14 , C12P19/16
CPC分类号： C12P19/14 , C08B1/00 , C12P7/10 , C12P19/02 , C12P19/04 , C12P19/16 , C12P2201/00 , C12P2203/00 , Y02E50/16

8. 发明公开

EP2809793A2 PROCESS FOR PRODUCING MALTITOL FROM STARCH 有权
标题翻译：用于生产麦芽糖醇，从强度
公开(公告)号：EP2809793A2
公开(公告)日：2014-12-10
申请号：EP13725757.2
申请日：2013-01-24
申请人： Cargill, Incorporated
发明人： FURLAN, Tiziano , NATALONI, Luigi , TOLOMELLI, Patrizia
IPC分类号： C12P19/12 , C12P19/14 , C12P19/16 , C12P19/22 , C07H15/04
CPC分类号： C12P19/22 , C07H15/04 , C12P19/12 , C12P19/14 , C12P19/16
摘要： The present invention relates to a process for producing a syrup which is rich in maltitol. The process is comprising the successive steps of liquefaction of a starch milk and saccharification of the liquefied starch milk in the presence of alpha-amylase, beta-amylase and a debranching enzyme selected from the group of pullulanase, iso-amylase and mixtures thereof, preferably pullulanase and further adding maltogenic alpha-amylase and/or iso-amylase, for obtaining a maltose containing syrup comprising at least 85% maltose based on dry matter and less than 1.5% glucose based on dry matter, preferably less than 1% glucose based on dry matter, followed by molecular sieving of the maltose containing syrup to obtain a fraction (A) comprising at least 95% maltose based on dry substance of fraction (A), and further hydrogenating catalytically fraction (A) for obtaining a liquid maltitol enriched product (B).

9. 发明公开

EP2687597A1 MODIFIED LPHA-GLUCOSIDASE AND APPLICATIONS OF SAME 审中-公开
标题翻译：修饰的LPHA-葡糖苷酶及其应用
公开(公告)号：EP2687597A1
公开(公告)日：2014-01-22
申请号：EP12758032.2
申请日：2012-03-05
申请人： Amano Enzyme Inc.
发明人： ISHIHARA Satoru
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/24 , C12P19/16 , C12P19/18
CPC分类号： C12N9/2408 , C12N9/1051 , C12N9/2428 , C12P19/04 , C12P19/16 , C12P19/18 , C12P19/20 , C12Y302/0102 , Y02P20/52
摘要： The object is to provide an α-glucosidase in which a transglycosylation activity predominates, and use thereof, and the like. A modified α-glucosidase consisting of an amino acid sequence in which one or two or more of the amino acid(s) selected from the group consisting of the following (1) to (14) has/have been substituted by other amino acid(s) in the amino acid sequence of α-glucosidase: (1) an amino acid corresponding to the amino acid at the position 385 of the amino acid sequence of SEQ ID NO.1; (2) an amino acid corresponding to the amino acid at the position 491 of the amino acid sequence of SEQ ID NO.1; (3) an amino acid corresponding to the amino acid at the position 535 of the amino acid sequence of SEQ ID NO.1; (4) an amino acid corresponding to the amino acid at the position 450 of the amino acid sequence of SEQ ID NO.1; (5) an amino acid corresponding to the amino acid at the position 534 of the amino acid sequence of SEQ ID NO.1; (6) an amino acid corresponding to the amino acid at the position 537 of the amino acid sequence of SEQ ID NO.1; (7) an amino acid corresponding to the amino acid at the position 538 of the amino acid sequence of SEQ ID NO.1; (8) an amino acid corresponding to the amino acid at the position 554 of the amino acid sequence of SEQ ID NO.1; (9) an amino acid corresponding to the amino acid at the position 556 of the amino acid sequence of SEQ ID NO.1; (10) an amino acid corresponding to the amino acid at the position 579 of the amino acid sequence of SEQ ID NO. 2; (11) an amino acid corresponding to the amino acid at the position 585 of the amino acid sequence of SEQ ID NO. 2; (12) an amino acid corresponding to the amino acid at the position 630 of the amino acid sequence of SEQ ID NO. 1; (13) an amino acid corresponding to the amino acid at the position 683 of the amino acid sequence of SEQ ID NO.1; and (14) an amino acid corresponding to the amino acid at the position 689 of the amino acid sequence of SEQ ID NO.1.
摘要翻译：其目的在于提供转糖基活性占优势的α-葡糖苷酶及其用途等。由选自下列（1）至（14）中的一个或两个以上氨基酸已经被其他氨基酸取代的氨基酸序列组成的修饰的α-葡糖苷酶（（1）对应于SEQ ID NO.1的氨基酸序列的第385位的氨基酸的氨基酸;（2）对应于SEQ ID NO.1的氨基酸序列的第385位氨基酸的氨基酸; （2）对应于SEQ ID NO.1氨基酸序列第491位氨基酸的氨基酸; （3）对应于SEQ ID NO.1氨基酸序列535位氨基酸的氨基酸; （4）对应于SEQ ID NO.1氨基酸序列第450位氨基酸的氨基酸; （5）对应于SEQ ID NO.1氨基酸序列534位氨基酸的氨基酸; （6）对应于SEQ ID NO.1氨基酸序列537位氨基酸的氨基酸; （7）对应于SEQ ID NO.1氨基酸序列538位氨基酸的氨基酸; （8）对应于SEQ ID NO.1氨基酸序列554位氨基酸的氨基酸; （9）对应于SEQ ID NO.1氨基酸序列556位氨基酸的氨基酸; （10）对应于SEQ ID NO.1的氨基酸序列第579位氨基酸的氨基酸。 2; （11）对应于SEQ ID NO.1的氨基酸序列的585位的氨基酸的氨基酸。 2; （12）对应于SEQ ID NO.1的氨基酸序列的第630位的氨基酸的氨基酸。 1; （13）对应于SEQ ID NO.1的氨基酸序列的第683位的氨基酸的氨基酸; 和（14）对应于SEQ ID NO.1氨基酸序列689位氨基酸的氨基酸。

10. 发明公开

EP2620506A1 Method of producing a galacto-oligosaccharide-containing composition 审中-公开
标题翻译： Verfahren zur Herstellung einer Galacto-Oligosaccharid-haltige Zusammensetzung
公开(公告)号：EP2620506A1
公开(公告)日：2013-07-31
申请号：EP12152502.6
申请日：2012-01-25
申请人： Arla Foods Amba
发明人： Bertelsen, Hans , Wejse, Peter Langborg , Busch, Jon Weis
IPC分类号： C12P19/00 , C12P19/04 , C12P19/16 , C12P19/20
CPC分类号： C12P19/14 , C12N9/2471 , C12P19/00 , C12P19/04 , C12Y302/01023
摘要： The present invention relates to a method of producing compositions containing galacto-oligosaccharides as well as to galacto-oligosaccharide-containing compositions as such.
摘要翻译：本发明涉及一种制备含有低聚半乳糖的组合物以及含有低聚半乳糖的组合物的方法。

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