发明公开
EP4321620A1 METHOD FOR PRODUCING TAGATOSE BY IMMOBILIZING MULTIPLE ENZYMES BY USING ARTIFICIAL OIL BODY
审中-实审
基本信息:
- 专利标题： METHOD FOR PRODUCING TAGATOSE BY IMMOBILIZING MULTIPLE ENZYMES BY USING ARTIFICIAL OIL BODY
- 申请号：EP22783792.9 申请日：2022-02-11
- 公开(公告)号：EP4321620A1 公开(公告)日：2024-02-14
- 发明人： MA, Yanhe , SHI, Ting , HAN, Pingping , LI, Yunjie
- 申请人： TIANJIN YEAHE BIOTECHNOLOGY CO., LTD
- 申请人地址： CN Tianjin, 300308 1-201-4, Building 8, Huiying Industrial Park No. 86 Zhonghuan West Road Tianjin Pilot Free Trade Zone (Airport Economic Area)
- 代理机构： Pharma Patents International AG
- 优先权： CN202110370762 20210407
- 国际公布： WO2022213721 20221013
- 主分类号： C12N11/18
- IPC分类号： C12N11/18 ; C12N11/06 ; C12P19/24 ; C12P19/18 ; C12P19/16 ; C12P19/02
摘要：
Provided are the immobilization of multiple enzymes on the basis of an artificial oil body and an application thereof in the preparation of tagatose. Specifically, an artificial oil body is used to mix an expressed fusion protein of target protease-oil body protein with an oil body, which then undergoes an ultrasonic treatment; the fusion protein is anchored to the surface of the oil body by means of the specific hydrophobicity of a human protein to form an artificial oil body containing the target protease, so that the purification and immobilization of enzymes can be completed simultaneously. The immobilized multiple enzymes that can be used for tagatose production utilize an artificial oil body as an immobilized enzyme substrate, which significantly improves the stability of the immobilized enzymes, reduces the production cost of the current enzymatic preparation of tagatose, and has a simple preparation process.