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1. 发明公开

EP0108301A3 Method of heterologous cloning of gene in bacillus microorganism 失效
标题翻译：基因在黑麦草微生物中的杂交克隆方法
公开(公告)号：EP0108301A3
公开(公告)日：1986-02-05
申请号：EP83110394
申请日：1983-10-19
申请人： MILES LABORATORIES, INC.
发明人： Grosch, Josephine , Wilson, Gary A. , Yehle, Clifford O. , Wollweber, Karen
IPC分类号： C12N15/00 , C12N09/28 , C12N09/34 , C12N09/54 , C12N09/92 , C12R01/07
CPC分类号： C12N9/2417 , C12N9/2428 , C12N9/54 , C12N9/92 , C12N15/64 , C12N15/75 , C12Q1/40
摘要： A method for heterologous cloning of a gene encoding α-amylase, protease, amyloglucosidase or glucose isomerase. The method involves the use of a bifunctional plasmid which can replicate in both E. coli and B. subtilis, and contains at least two selectable markers. A gene fragment for the enzyme is incorporated into the plasmid, leaving at least one functional selectable marker and a nonfunctional marker, producing a mixture of recombinant hybrid plasmids and the original bifunctional plasmid. This mixture is incorporated into E. coli microorganisms and transformants seiected. The transformants which contain the recombinant plasmid are identified, and plasmid DNA extracted. The plasmid DNA is used to transform Bacillus host microorganisms and transformants containing the recombinant plasmids are selected by means of the functional selectable marker.

2. 发明公开

EP0268193A3 Process for promoting the excretion of amylolytic enzymes from bacteria, and enzymes produced according to this process 失效
标题翻译：促进从细菌中提取淀粉酶的方法和根据本方法生产的酶
公开(公告)号：EP0268193A3
公开(公告)日：1988-08-24
申请号：EP87116625
申请日：1987-11-11
申请人： Kali-Chemie Aktiengesellschaft
发明人： Antranikian, Garabed, Dr. , Gottschalk, Gerhard, Prof. Dr.
IPC分类号： C12N09/24 , C12N09/28 , C12N09/34 , C12N09/44
CPC分类号： C12Y302/01003 , C12N9/2417 , C12N9/2428 , C12N9/2457 , C12Y302/01001 , C12Y302/01041
摘要： Die Erfindung betrifft ein Verfahren zur Förderung der Ex kretion von amylolytischen Enzymen aus thermophilen, stärke abbauenden, die genannten Enzyme bildenden anaeroben Bakte rien sowie die Enzyme, die nach dem Verfahren erhältlich sind, nämlich Pullulanasen, alpha-Amylase und Glucoamylase.

3. 发明公开

EP0135138A3 A novel thermostable glucoamylase and method for its production 失效
标题翻译：一种新型热稳定蛋白酶及其生产方法
公开(公告)号：EP0135138A3
公开(公告)日：1986-06-25
申请号：EP84109640
申请日：1984-08-13
申请人： CPC INTERNATIONAL INC.
发明人： Katkocin, Dennis M. , Word, Nancy S. , Yang, Shiow-Shong
IPC分类号： C12N09/34 , C12P19/20 , C12N15/00 , C12R01/145
CPC分类号： C12N9/2428 , Y10S435/842
摘要： This invention relates to a glucoamylase enzyme exhibiting thermostability at pH values between 6 and 7 which is derived from a spore-forming, thermophilic, anaerobic bacterium and to a process for its production. The glucoamylase is especially useful for the preparation of glucose-containing syrups from starch.

4. 发明公开

EP0260404A3 Amylolytic enzymes producing microorganisms constructed by recombinant DNA technology and their use for fermentation processes 失效
标题翻译：生产由重组DNA技术构建的微生物的氨基酸酶及其用于发酵过程的用途
公开(公告)号：EP0260404A3
公开(公告)日：1988-07-06
申请号：EP87110370
申请日：1987-07-17
申请人： Heineken Technisch Beheer B.V.
发明人： Strasser, Alexander , Martens, Feodor Bernard , Dohmen, Jürgen , Hollenberg, Cornelius P.
IPC分类号： C12N15/00 , C12P21/02 , C12N09/30 , C12N09/34 , C12P07/06 , C12P01/00 , C12C11/04 , C12N01/16 , A21D08/04 , C12N01/18
CPC分类号： C12N9/242 , A21D8/047 , C12C12/004 , C12C12/006 , C12C12/02 , C12N9/2428 , C12N15/81 , C12N15/815 , C12P7/06 , Y02E50/17
摘要： This invention provides a method for producing amylolytic enzymes by culturing a microorganism, having received as a result of recombinant DNA technology DNA sequences from a donor yeast comprising the coding sequences for the amylolytic enzymes wherein the host microorgamism is capable of expressing said amylolytic enzymes. Furthermore, this invention provides microorganisms genetically engineered as to being able to produce and express the amylolytic enzymes, a vector containing the DNA sequences, coding for the amylolytic enzymes and the respective DNA sequences. The said host microorganisms are useful in the production of biomass and many fermentation processes, preferably in the production of special beers.

5. 发明公开

EP0146022A3 Recovery of acid fungal protease 失效
标题翻译：恢复酸性真菌蛋白
公开(公告)号：EP0146022A3
公开(公告)日：1987-07-22
申请号：EP84114313
申请日：1984-11-27
申请人： MILES LABORATORIES, INC.
发明人： Shetty, Jayarama K. , Marshall, James J.
IPC分类号： C12N09/62 , C12N09/34
CPC分类号： C12N9/2428 , C12N9/62 , Y10S435/814
摘要： Glucoamylase and acid fungal protease co-produced by the fermentation of fungal species capable of producing these enzymes are separated by carrying out the fermentation at a pH at which the protease associates itself with the fungal mycelium and removing the mycelium from the fermentation broth. The mycelium is then resuspended in an aqueous medium and the pH adjusted to cause the disassociation of the protease and mycelium with subsequent recovery of the protease from the aqueous medium.

6. 发明公开

EP0126206A3 Glucoamylase cDNA 失效
标题翻译：葡糖淀粉酶cDNA
公开(公告)号：EP0126206A3
公开(公告)日：1985-03-06
申请号：EP84100834
申请日：1984-01-26
申请人： CETUS CORPORATION
发明人： Nunberg, Jack H. , Flatgaard, Jeffrey E. , Innis, Michael Alan , Gelfand, David Harrow , Meade, James Henry
IPC分类号： C12N15/00 , C12P21/02 , C07C103/52 , C07H21/04 , C12P07/06 , C12N09/34 , C12N01/16 , C12R01/85
CPC分类号： C12N9/2428 , C07K2319/02 , C07K2319/036 , C12N15/10 , C12N15/70 , C12N15/81 , C12P7/06 , Y02E50/17
摘要： A gene having a DNA sequence complementary to that of the glucoamylase polypeptide mRNA from a fungal species, preferably Aspergillus awamori, is prepared. The mRNA is an approximately 2.2 kilobase poly a RNA obtained from fungal cells grown under conditions of glucoamylase induction. Reverse transcription of the mRNA provides a glucoamylase probe used to identify genomic digest fragments containing glucoamylase gene regions, which are sequenced to locate the introns and exons. The genomic fragments are spliced together to form a gene having a DNA sequence with altered or deleted introns which codes for fungal glucoamylase protein and is capable, when correctly combined with a cleaved DNA expression vector, of expressing a non-native protein having glucoamylase enzyme activity upon transformation of a host organism by the vector. The host organism is preferably a bacterium or a yeast. The transformed host organism may be used to produce ethanol.
摘要翻译：制备具有与来自真菌物种，优选泡盛曲霉的葡糖淀粉酶多肽mRNA互补的DNA序列的基因。 mRNA是从在葡糖淀粉酶诱导条件下生长的真菌细胞获得的约2.2千碱基多聚RNA。 mRNA的逆转录提供了用于鉴定含有葡糖淀粉酶基因区域的基因组消化片段的葡糖淀粉酶探针，其被测序以定位内含子和外显子。将基因组片段拼接在一起以形成具有编码真菌葡糖淀粉酶蛋白并具有改变或缺失的内含子的DNA序列的基因，并且当其与切割的DNA表达载体正确结合时能够表达具有葡糖淀粉酶酶活性的非天然蛋白通过载体转化宿主生物体。宿主生物优选为细菌或酵母。转化的宿主生物体可用于生产乙醇。

7. 发明公开

EP0263372A3 Method for the preparation of a hydrophobic enzyme-containing composition and the produced thereby 失效
标题翻译：含有疏水酶的组合物的制备方法及其生产方法
公开(公告)号：EP0263372A3
公开(公告)日：1988-08-03
申请号：EP87114016
申请日：1987-09-25
申请人： MILES LABORATORIES, INC.
发明人： Chung, Koo-Heung , Verhoff, Francis Henry
IPC分类号： C12N09/00 , C12N09/96 , C12N09/34 , C12N09/18
CPC分类号： C12N9/2428 , C12N9/00 , C12N9/18 , C12N9/96
摘要： An enzyme-containing solid composition is prepared by a process comprising removing the solvent from a mixture of a water-soluble enzyme, a water-insoluble metal salt of a fatty acid and an organic solvent.

8. 发明公开

EP0185327A3 Process for producing alcohol 失效
标题翻译：生产酒精的方法
公开(公告)号：EP0185327A3
公开(公告)日：1987-07-15
申请号：EP85115912
申请日：1985-12-13
申请人： Suntory Limited
发明人： Ashikari, Toshihiko , Nakamura, Norihisa , Tanaka, Yoshikazu , Shibano, Yuji , Yoshizumi, Hajime
IPC分类号： C12N15/00 , C12N09/34 , C12P07/06
CPC分类号： C12N9/2428 , C12P7/06 , Y02E50/17 , Y10S435/939
摘要： This invention provides a process for producing ethanol from a starchy material, wherein the starchy material is simultaneously subjected to saccharification and subsequent fermentation by use of a yeast host which has been transformed by an expressible recombinant vector comprising a glucoamylase gene derived from a fungus of the genus Rhizopus

9. 发明公开

EP0171218A3 Enzymatic hydrolysis of granular starch directly to glucose 失效
标题翻译：颗粒淀粉的直接水解至葡萄糖
公开(公告)号：EP0171218A3
公开(公告)日：1987-06-03
申请号：EP85305167
申请日：1985-07-19
申请人： GENENCOR, INC.
发明人： Jackson, Leroy Eugene , Seidman, Martin
IPC分类号： C12N09/34 , C12P19/20
CPC分类号： C12N9/2428 , C12P19/20 , C12Y302/01003
摘要： It is discovered that certain fungi, particularly members of the genus Humicola, secrete an enzyme mixture or preparation that hydrolyzes raw or granular starch directly to glucose without producing substantial amounts of limit dextrins or tri or higher number saccharides. The fungi is germinated and fermented on carbohydrate substrates, producing a broth exhibiting raw starch hydrolyzing activity. The broth is separated from the fungi annd is added to a slurry of raw starch to hydrolyze the same to glucose. To produce glucose more efficiently, mutuations have been induced in Humicola, and certain mutants produce elevated titers of raw starch hydrolyzing activity.

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