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    • 4. 发明公开
    • Laser scanning microscope
    • 激光扫描-Mikroskop
    • EP2312367A1
    • 2011-04-20
    • EP10013319.8
    • 2010-10-05
    • Olympus Corporation
    • Yamashita, YusukeNakata, Tatsuo
    • G02B21/00G02B21/28G02B21/30G01N21/64
    • G02B21/0076G02B21/0088G02B21/28G02B21/30
    • Provided is a laser scanning microscope that allows stable examination by eliminating the influence of ambient light while inhibiting damage to a specimen and fading of fluorescence. Employed is a laser scanning microscope 1 including a culture vessel 6 accommodating a specimen A and capable of maintaining an interior temperature and humidity thereof and an optical system space 5 adjacent to the culture vessel 6 and optically connected to the culture vessel 6. The optical system space 5 includes a scanner 22 that two-dimensionally scans ultrashort pulsed laser light across the specimen A; an objective lens 15 that focuses the scanned ultrashort pulsed laser light on the specimen A and collects light coming from the specimen A; a dichroic mirror 53, disposed between the scanner 22 and the objective lens 15, that splits off the light coming from the specimen A from the laser light; a photodetector 55 that detects the split-off light coming from the specimen A; and a outer cover 2, provided so as to surround the optical system space 5, that blocks light coming from outside the optical system space 5.
    • 提供了一种激光扫描显微镜,其通过消除环境光的影响,同时抑制样品的损伤和荧光褪色,从而进行稳定的检查。 采用激光扫描显微镜1,其包括容纳样本A并能够保持其内部温度和湿度的培养容器6和与培养容器6相邻并光学连接到培养容器6的光学系统空间5。 空间5包括扫描仪22,其将超短脉冲激光二维扫描穿过样本A; 物镜15,其将扫描的超短脉冲激光聚焦在样本A上并收集来自样本A的光; 设置在扫描器22和物镜15之间的分色镜53,其从激光分离出来自检体A的光; 检测来自检体A的分离光的光检测器55; 以及设置为围绕光学系统空间5设置的外盖2,其遮挡来自光学系统空间5的外部的光。