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1. 发明公开

EP2318507A1 METHOD, KIT AND SYSTEM FOR CULTURABLE CELL COUNT 审中-公开
标题翻译：方法，试剂盒和系统计数细胞培养的
公开(公告)号：EP2318507A1
公开(公告)日：2011-05-11
申请号：EP09787468.9
申请日：2009-07-09
申请人： Tacount Exact Ltd.
发明人： GLUKHMAN, Vladimir , KAPLAN, Elon
IPC分类号： C12M1/34 , G01N33/50
CPC分类号： C12Q1/06 , C12M41/36
摘要： The present invention provides a method, kit and system for determining a quantitative value indicative of the number of culturable microbial cells, such as
E.Coli, Ps. Aeroginosa or a mixture of microbial cells (e.g. for total microbial count) in a tested sample, the method comprising (i) contacting the tested sample, susceptible of carrying culturable microbial cells with at least one signal-emitting agent capable of associating with the microbial cell's membrane, said contacting is for a predetermined first time period (Tl) being sufficient for internalization of the signal-emitting agent into the microbial cell to a level at which the signal emitted from the sample essentially reaches a plateau; (ii) removing from said tested sample non-internalized signal emitting agent; (iii) during a second time period (T2), following said first time period (Tl), and at which said signal is essentially maintained in said plateau, detecting from signal emitting objects within said sample signal emitting culturable cells, said detection being based on selection parameters predetermined for said culturable cells; and (iv) determining, based on said selected signal emitting objects, a quantitative value indicative of the number of culturable cells in the tested sample. The kit comprises one or more of said signal emitting agents and instructions for use of same. Also provided is a program storage device readable by machine, tangibly embodying a program of instructions executable by the machine to perform said method and a computer program comprising computer program code means for performing said method.

2. 发明公开

EP3102693A1 APPARATUS, SYSTEM AND METHOD FOR LIVE BACTERIA MICROSCOPY 审中-公开
标题翻译：设备，系统和方法观察活体细菌
公开(公告)号：EP3102693A1
公开(公告)日：2016-12-14
申请号：EP15708602.6
申请日：2015-02-05
申请人： Tacount Exact Ltd.
发明人： GLUKHMAN, Vladimir , AZRAD, Erez
IPC分类号： C12Q1/06 , G02B21/28 , G02B21/26 , G01N1/40 , G01N15/14
CPC分类号： C12Q1/06 , C12Q1/04 , C12Q2304/00 , G01N1/4077 , G01N15/1456 , G01N2001/4088 , G02B21/26 , G02B21/28 , G02B21/362
摘要： A system for detecting microbial cells in a sample includes an apparatus configured to image at least one cell in the sample, and a cooling device. The apparatus includes a holder having an internal portion and an external portion which are configured so as to secure a membrane between the portions, and an imaging device disposed above the external portion and configured to permit examination of the at least one cell. The apparatus further includes a stage attachable to a stage platform configured to connect to a motor, and a projecting member projecting from an upper surface of the stage and configured to receive and exchange solution. The cooling device includes a thermoelectric cooling element and/or at least one tube configured to circulate a cooling medium beneath the stage so as to cool the sample.

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