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1. 发明授权

EP1389305B1 PEPTIDE FRAGMENTATION 有权
标题翻译：肽碎片
公开(公告)号：EP1389305B1
公开(公告)日：2009-09-30
申请号：EP02739339.6
申请日：2002-05-20
申请人： GE Healthcare Bio-Sciences AB
发明人： LIMINGA, Maria c/o Amersham Biosciences AB , MALOISEL, Jean-Luc Amersham Pharmacia Biotech AB , PALMGREN, Ronnie c/o Amersham Pharmacia Biotech AB , KEOUGH, Thomas, W. The Procter and Gamble Company , YOUNGQUIST, Robert S. The Procter and Gamble Company , VAUGHN, Harold, L. The Procter and Gamble Company , YELM, Ken, E. c/o The Procter and Gamble Company
IPC分类号： G01N33/58 , C12Q1/37 , C07D207/48
CPC分类号： C07D207/46 , G01N33/58 , G01N33/6824 , G01N2458/15 , Y10T436/18
摘要： The present invention relates to a method of identifying a polypeptide using a class of novel, water-stable reagents. More specifically, the method according to the invention comprises the steps of (a) derivatization in an aqueous solution of the N-terminus of the polypeptide, or the N-termini of one more peptides of the polypeptide, with at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives; (b) analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern; and (c) interpreting the fragmentation pattern. Furthermore, the present invention also relates to a kit and novel reagents for use in the present method.
摘要翻译：本发明涉及使用一类新型水稳定试剂鉴定多肽的方法。更具体地说，根据本发明的方法包括以下步骤：（a）在多肽的N末端或多肽的一个或多个肽的N末端的水溶液中用至少一种酸性试剂进行衍生化，包含与活化的酸部分偶联的磺酰基部分以提供一种或多种肽衍生物; （b）使用质谱技术分析至少一个这样的衍生物以提供分裂模式; 和（c）解释分裂模式。此外，本发明还涉及用于本方法的试剂盒和新型试剂。

2. 发明公开

EP1362058A2 COMPARATIVE LIGAND MAPPING FROM MHC POSITIVE CELLS 有权
标题翻译：比较配体从图片MHC阳性细胞
公开(公告)号：EP1362058A2
公开(公告)日：2003-11-19
申请号：EP01981521.6
申请日：2001-10-10
申请人： The Board of Regents of The University of Oklahoma
发明人： HICKMAN, Heather , HILDEBRAND, William
IPC分类号： C07K14/00
CPC分类号： G01N33/6878 , A61K39/39 , A61K2039/605 , A61K2039/622 , C07K14/005 , C07K14/47 , C07K14/4702 , C07K14/4728 , C07K14/70539 , C07K14/70571 , C07K14/78 , C07K16/2833 , C07K2319/00 , C12N9/1247 , C12N9/6421 , C12N2740/16122 , G01N33/5008 , G01N33/502 , G01N33/5044 , G01N33/5091 , G01N33/569 , G01N33/6824 , G01N33/6848 , G01N2333/16 , G01N2333/70539
摘要： The present invention relates generally to a methodology for the isolation, purification and identification of peptide ligands presented by MHC positive cells. In particular, the methodology of the present invention relates to the isolation, purification and identification of these peptide ligands from soluble class I and class II MHC molecules which may be uninfected, infected, or tumorgenic. The methodology of the present invention broadly allows for these peptide ligands and their comcomittant source proteins thereof to be identified and used as markers for infected versus uninfected cells and/or tumorgenic versus nontumorgenic cells with said identification being useful for marking or targeting a cell for therapeutic treatment or priming the immune response against infected cells.

3. 发明公开

EP1060377A2 REAGENTS AND METHODS FOR PROTEIN MICROSEQUENCING 审中-公开
标题翻译：试剂和蛋白质微量测序方法
公开(公告)号：EP1060377A2
公开(公告)日：2000-12-20
申请号：EP99931744.9
申请日：1999-02-26
申请人： Biotraces Inc.
发明人： WILK, Andrzej , DRUKIER, Andrzej, K.
IPC分类号： G01N1/00
CPC分类号： G01N33/6824 , C07K1/12 , G01N33/60
摘要： The invention provides methods of peptide and protein microsequencing using radiolabeled reagents to perform peptide and protein microsequencing at levels lower than 10 femtomole, preferably at a level lower than 1 femtomole.

4. 发明授权

EP0549752B1 PROTEIN SEQUENCING 失效
标题翻译： PROTEINSEQUENZIERUNG
公开(公告)号：EP0549752B1
公开(公告)日：1999-03-17
申请号：EP92914016.8
申请日：1992-06-12
申请人： Beckman Coulter, Inc.
发明人： FARNSWORTH, Vincent
IPC分类号： C07K1/12 , G01N33/68 , C07C333/04
CPC分类号： G01N33/6824 , C07K1/128 , Y10S530/802
摘要： A method is described for the generation of phenylthiocarbamyl. (PTC) amino acids from phenylthiohydantoin (PTH) or anilinothiozolinone (ATZ) amino acids. The method involves a base-catalyzed ring opening of the PTH or ATZ in the presence of a reducing agent. The method affords an alternative to the established aqueous acid conversion reaction of the Edman degradation in which ATZ and PTC amino acids are converted to the PTH amino acid. A further method is described for the generation of reactive ATZ amino acids from PTH amino acids. These methods facilitate the analysis of protein at low molar amounts by allowing the synthesis of amino acid derivatives which can be analyzed in quantities which are much lower than those required for conventional PTH amino acid analysis.

5. 发明公开

EP0669002A1 N-TERMINAL SEQUENCING OF PROTEINS AND PEPTIDES 失效
标题翻译：蛋白质和肽的N-末端测序。
公开(公告)号：EP0669002A1
公开(公告)日：1995-08-30
申请号：EP94929836.0
申请日：1994-09-14
申请人： CITY OF HOPE
发明人： BAILEY, Jerome M. , SHIVELY, John, E.
IPC分类号： C07K1 , G01N33
CPC分类号： C07K1/128 , G01N33/6824
摘要： An improved method for the N-terminal sequential degradation of proteins and peptides is disclosed. The protein or peptide to be sequenced is reacted with a compound effective to impart a tertiary amine functionality to the thiazolinone derivative of a cleaved terminal amino acid of the peptide.

6. 发明公开

EP0538999A2 Amino acid sequence determination with mobile peptide 失效
标题翻译： Bestimm der derAminosäuresequenzmit einem mobilen Peptid。
公开(公告)号：EP0538999A2
公开(公告)日：1993-04-28
申请号：EP92307683.0
申请日：1992-08-21
申请人： Hewlett-Packard Company
发明人： Kohr, William J.
IPC分类号： G01N33/68 , G01N30/38 , B01J19/00
CPC分类号： G01N33/6824 , C07K1/128 , Y10S530/81 , Y10S530/817
摘要： Sequential degradation of peptides for sequencing purposes by successive cleavage of amino acids from one end of the peptide chain is performed in an adsorption column with flows of the degradation reagents and wash liquids passing through the column in both directions. Migration of the peptide in one direction is thereby compensated by a subsequent migration in the other, and loss of peptide from the column over repeated cleavages is avoided. In preferred embodiments, the column contains two serially arranged adsorbent zones with differing adsorption characteristics, and the directions of flow of the various system components through the column are selected with a view toward a difference in peptide partitioning between the stationary and mobile phases in one zone vs . the other. The result is that any migration of the peptide occurring at any stage of a given cycle of the procedure occurs at a greater rate toward the zone interface than away from it. The overall effect after repeated cycles is a focusing of the peptide toward the center of the column without the need for covalent bonding of the peptide to a solid support.
摘要翻译：通过连续从肽链一端切割氨基酸的肽进行顺序降解，在吸附柱中进行，其中降解试剂和洗涤液体在两个方向上通过该柱。因此肽在一个方向的迁移由另外的随后的迁移补偿，并且避免了反复裂解从柱中的肽的损失。在优选的实施方案中，该柱含有两个具有不同吸附特性的连续排列的吸附剂区，并且通过该柱选择各种系统组分的流动方向，以便在一个区域中固定相和流动相之间的肽分配差异相对于另一个结果是，在程序的给定循环的任何阶段发生的肽的任何迁移以比远离它的区域界面更大的速率发生。重复循环后的总体效应是将肽聚焦到柱的中心，而不需要将肽与固体支持物共价键合。

7. 发明授权

EP0202894B1 A method for detecting amino acid derivatives 失效
标题翻译：检测氨基酸衍生物的方法
公开(公告)号：EP0202894B1
公开(公告)日：1992-02-19
申请号：EP86303763.6
申请日：1986-05-16
申请人： SEIKO INSTRUMENTS INC.
发明人： Tsugita, Akira , Arai, Isamu , Ataka, Tatsuaki Seiko Instr.&Electronics Ltd.
IPC分类号： G01N33/68
CPC分类号： C07K1/12 , G01N33/533 , G01N33/534 , G01N33/6809 , G01N33/6824 , Y10T436/141111

8. 发明公开

EP0397813A1 METHOD FOR SEQUENCING PEPTIDES OR PROTEINS 失效
标题翻译：肽或蛋白质的序列。
公开(公告)号：EP0397813A1
公开(公告)日：1990-11-22
申请号：EP89911635.0
申请日：1989-10-24
申请人： PHARMACIA AB
发明人： AUFFRET, Anthony, David , AITKEN, Alastair , FARRIES, Timothy, Charles
IPC分类号： C12Q1 , G01N33
CPC分类号： G01N33/6842 , C12Q1/37 , G01N33/6824
摘要： L'invention concerne un procédé de détermination de séquences d'acides aminés, consistant à acyler l'acide aminé à terminaison N puis à le cliver de manière sélective à l'aide d'une acylamino hydrolase à des fins d'analyse. On répète ce cycle pour chaque acide aminé se trouvant dans la protéine ou le peptide à analyser.

9. 发明公开

EP0257735A2 Devices and method for amino acid sequence determination 失效
标题翻译： Geräteund Verfahren zur Bestimmung der Sequenz vonAminosäuren。
公开(公告)号：EP0257735A2
公开(公告)日：1988-03-02
申请号：EP87304996.9
申请日：1987-06-05
申请人： GENENTECH, INC.
发明人： Kohr, William Jack
IPC分类号： G01N33/48 , G01N33/68 , G01N30/00 , G01N30/60
CPC分类号： G01N33/6824
摘要： A device for the determination of amino acid sequence of a polypeptide comprises two new features offering great advantages in the cost and efficiency of operation of amino acid sequencers: The sequencer is provided with the capability for the bidirectional flow of sequencing reagents and contains a sample chamber having a bicompositional adsorbent for the polypeptide.
摘要翻译：用于确定多肽的氨基酸序列的装置包括两个新特征，其在氨基酸测序仪的操作的成本和效率方面具有很大的优点：定序器具有测序试剂双向流动的能力并且包含样品室具有多肽的双组分吸附剂。

10. 发明公开

EP3371194A1 PROTEIN SEQUENCING METHODS AND REAGENTS 有权
公开(公告)号：EP3371194A1
公开(公告)日：2018-09-12
申请号：EP16854691.9
申请日：2016-10-17
申请人： Emili, Andrew , The Governing Council of the University of Toronto
发明人： Emili, Andrew
IPC分类号： C07D493/10 , C40B20/04 , G01N33/58 , G01N33/68
CPC分类号： G01N33/6824 , C07D493/10 , C40B20/04 , Y02P20/55
摘要： Described are optical methods and reagents for sequencing polypeptides. A probe that exhibits different spectral properties when conjugated to different N-terminal amino acids is conjugated to the N-terminal amino acid of a polypeptide. Sequentially detecting one or more spectral properties of the probe conjugated to the N-terminal amino acid and cleaving the N-terminal amino acid produces sequence information of the polypeptide. The use of super-resolution microscopy allows for the massively parallel sequencing of individual polypeptide molecules in situ such as within a cell. Also described are probes comprising hydroxymethyl rhodamine green, an isothiocyanate group and a protecting group.

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