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    • 4. 发明公开
    • PECTIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF
    • 维生素DAVON的PEKTINABBAUENDE ENZYME AUS MACROPHOMINA PHASEOLINA
    • EP2885407A2
    • 2015-06-24
    • EP13829902.9
    • 2013-08-15
    • Bangladesh Jute Research Institute
    • ALAM, MaqsudulISLAM, Mohammed, ShahidulHOSSEN, Mohammed, MosaddequeHAQUE, Mohammed, SamiulALAM, Mohammed, Monjurul
    • C12N9/88
    • C12N9/88C12N9/18C12N9/2405C12Y301/01011C12Y402/02002C12Y402/02023
    • The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus Macrophomina phaseolina (“M. phaseolina”), responsible for degrading pectin, and it comprises and/or consists of nucleotide sequences set forth in SEQ ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, with or having enhanced production of pectin degrading enzyme. The polypeptide of the invention can be used for, amongst other things, manufactured fruit juice, textile products, pulp and paper, coffee, tea and oil extraction and pectic waste water treatment.
    • 本发明公开了编码酶的分离的多核苷酸,其衍生自负责降解果胶的巨噬细胞相容酶真菌(“M. phaseolina”),并且其包含和/或由SEQ ID NO:1所示的核苷酸序列组成。 编号2,5,8,11,14,17,20,23,26,29,32,35,38,41,44,47,50,53,56,59,62,1,4,7 ,10,13,16,19,22,25,28,31,34,37,40,43,46,49,52,55,58和61,或这些序列的补体。 本发明还涉及由SEQ ID No.3,6,9,12,15,18,21,27,23,30,33,36,39,42,45,45,46,45,45,48,48,48,48,48,48,48,45,45,40,45,45,45,40,45,40,45,40,49,40,49,40,49,40,49,40,49,40,49,40,49,40 48,51,54,57,60及63条; 包含该多核苷酸的重组基因构建体; 转化体和转基因真菌,其包含具有或具有增强的果胶降解酶生产的重组基因构建体。 本发明的多肽可以用于制造果汁,纺织品,纸浆和纸,咖啡,茶和油提取和果胶废水处理。