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1. 发明公开

EP2931894A4 NUCLEOTIDE SEQUENCE ENCODING HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT22 (HD-ZIP PROTEIN 22) FROM CORCHORUS OLITORIUS AND CORCHORUS CAPSULARIS AND METHODS OF USE 审中-公开
标题翻译： NUKLEOTIDSEQUENZCODIERENDES同源盒白介素-ZIP蛋白HAT22（HD-ZIP蛋白22）黄麻关长蒴黄麻和capsularis和使用方法
公开(公告)号：EP2931894A4
公开(公告)日：2016-05-25
申请号：EP14859332
申请日：2014-11-21
申请人： BANGLADESH JUTE RES INST
发明人： ALAM MAQSUDUL , ISLAM MOHAMMED SHAHIDUL , AHMED BORHAN , HAQUE MOHAMMED SAMIUL , ALAM MOHAMMED MONJURUL
IPC分类号： C12N15/82 , C07K14/415 , C12N15/29
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： The present invention relates to the isolated polynucleotide encoding homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22) from the plants Corchorus olitorius and Corchorus capsularis and corresponding polypeptide derived thereof. The present invention also relates to the plants having modulated expression of a nucleic acid encoding a homeobox-leucine zipper HAT22 polypeptide or a homologue thereof, which has the ability to modify, preferably to increase/enhance, the fiber length, plant height, and/or plant biomass. More specifically, the invention relates to polypeptides having homeobox-leucine zipper protein HAT22 activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. The present invention further provides vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22). The invention also provides methods for producing the said protein and methods for modifying the said protein in order to improve their desirable characteristics. The said protein of the invention can be used in a variety of ways, including increasing/enhancing the fiber length, height and biomass of plants and fiber yield.
摘要翻译：本发明涉及从植物长蒴黄麻和黄麻capsularis以及它们的衍生相应的多肽的分离的多核苷酸编码同源异型框亮氨酸拉链蛋白HAT22（HD-ZIP蛋白22）。因此，本发明涉及具有编码核酸的同源异型框亮氨酸拉链HAT22多肽或其同源物，其具有修改，优选地，以增加/提高的能力，纤维长度，株高的调节的表达的植物，和/ 或植物生物量。更具体地，本发明涉及具有同源框亮氨酸拉链蛋白HAT22活性，多核苷酸编码所述多肽的合成，以及制备和使用这些多核苷酸和多肽的方法中的多肽。本发明还提供了载体，表达构建体和宿主细胞，其包括和/或所述同源框亮氨酸拉链蛋白HAT22（HD-ZIP蛋白22）的核苷酸序列组成的聚合物。因此本发明提供了生产所述蛋白和用于为了提高其理想特性修改所述蛋白质的方法的方法。本发明的所述蛋白可以以各种方式，包括增加/增强植物和纤维产量的纤维长度，高度和生物量使用。

2. 发明公开

EP2922961A4 NUCLEOTIDE SEQUENCE ENCODING WUSCHEL-RELATED HOMEOBOX4 (WOX4) PROTEIN FROM CORCHORUS OLITORIUS AND CORCHORUS CAPSULARIS AND METHODS OF USE FOR SAME 有权
标题翻译： WUSCHEL ASSOCIATED HOMEOBOX4（WOX4）蛋白来自长长蒴黄麻和capsularis和方法及其使用核苷酸编码
公开(公告)号：EP2922961A4
公开(公告)日：2015-12-30
申请号：EP14859333
申请日：2014-11-20
申请人： BANGLADESH JUTE RES INST
发明人： ALAM MAQSUDUL , ISLAM MOHAMMED SHAHIDUL , AHMED BORHAN , HAQUE MOHAMMED SAMIUL , ALAM MOHAMMED MONJURUL
IPC分类号： C12N15/82 , A01H5/10 , C07K14/415
CPC分类号： C12N15/8242 , C07K14/415 , C12N15/8218 , C12N15/8246 , C12N15/8261
摘要： The present invention discloses isolated polynucleotides encoding WUSCHEL-related homeobox4 proteins from two species of jute plants, namely, the Corchorus olitorius (“C. olitorius”) and Corchorus capsularis (“C. capsularis”), and corresponding polypeptides derived therefrom. The disclosed polynucleotide sequences encode WUSCHEL-related homeobox4 polypeptides (WOX4), which possess catalytic activities in enhancing fiber production in jute. The present invention also relates to the plants having a modulated expression of a nucleic acid encoding a WOX4 polypeptide, which have enhanced fiber yield relative to corresponding wild type plants or other control plants. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the protein are also provided. Also disclosed are methods for producing the proteins and methods for modifying the proteins in order to improve their desirable characteristics. The proteins of the invention can be used in a variety of ways, including inducing, initiating, improving, or enhancing plant growth, plant height, fiber and seed yield.
摘要翻译：本发明的盘以下分离的多核苷酸编码WUSCHEL来自两个物种的植物黄麻，即长蒴黄麻（“C.蒴黄麻”）和黄麻capsularis（“C. capsularis”），并且相应的多肽由其衍生的相关homeobox4蛋白质。盘多核苷酸游离缺失序列编码WUSCHEL相关homeobox4多肽（WOX4），这在提高黄麻纤维生产具有催化活性。因此，本发明涉及一种具有一个核酸编码多肽WOX4，其具有增强的纤维产量相对于对应的野生型植物或其他对照植物的受调节的表达植物。载体，表达构建体和宿主细胞，其包括和/或蛋白质的核苷酸序列由......因此被提供。所以光盘游离缺失是产生蛋白质的，以提高他们的期望特征改变蛋白质的方法和方法。在本发明的蛋白可以以各种方式，包括诱导，发起，改善或增强的植物生长，植物高度，纤维和种子产量可以使用。

3. 发明公开

EP2885407A4 PECTIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 有权
标题翻译：果胶降解从Macrophomina phaseolina酶及其用途
公开(公告)号：EP2885407A4
公开(公告)日：2016-05-11
申请号：EP13829902
申请日：2013-08-15
申请人： BANGLADESH JUTE RES INST
发明人： ALAM MAQSUDUL , ISLAM MOHAMMED SHAHIDUL , HOSSEN MOHAMMED MOSADDEQUE , HAQUE MOHAMMED SAMIUL , ALAM MOHAMMED MONJURUL
IPC分类号： C12N9/88
CPC分类号： C12N9/88 , C12N9/18 , C12N9/2405 , C12Y301/01011 , C12Y402/02002 , C12Y402/02023
摘要： The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus Macrophomina phaseolina (“M. phaseolina”), responsible for degrading pectin, and it comprises and/or consists of nucleotide sequences set forth in SEQ ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, with or having enhanced production of pectin degrading enzyme. The polypeptide of the invention can be used for, amongst other things, manufactured fruit juice, textile products, pulp and paper, coffee, tea and oil extraction and pectic waste water treatment.

4. 发明公开

EP2885404A4 LIGNIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 有权
标题翻译： LIGNINABBAUENDE ENZYME AUS MACROPHOMINA PHASEOLINA UND VERWENDUNGEN DAVON
公开(公告)号：EP2885404A4
公开(公告)日：2016-05-11
申请号：EP13829529
申请日：2013-08-15
申请人： BANGLADESH JUTE RES INST
发明人： ALAM MAQSUDUL , ISLAM MOHAMMED SHAHIDUL , HOSSEN MOHAMMED MOSADDEQUE , HAQUE MOHAMMED SAMIUL , ALAM MOHAMMED MONJURUL
IPC分类号： C12N9/08 , C07H21/04 , C07K14/37 , C12N15/53
CPC分类号： C12N9/0061 , C12N9/0065
摘要： The present invention relates to the field of plant breeding and disease resistance in respect of lignin degradation by Macrophomina phaseolina (“M. phaseolina”). Disclosed are isolated polynucleotides encoding the lignin degrading enzymes produced by Macrophomina phaseolina, or the complement of such sequences. Also disclosed are isolated polypeptides encoded by various polynucleotide sequences; a recombinant gene construct comprising a polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, having enhanced production of lignin degrading enzyme. Further disclosed are polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomerase to fermentable sugar in saccharification process of biomass.
摘要翻译：本发明涉及植物育种领域和关于通过Macrophomina phaseolina（“M. phaseolina”）的木质素降解的抗病性。公开的是分离的多核苷酸，其编码由Macrophomina phaseolina产生的木质素降解酶，或这些序列的补体。还公开了由各种多核苷酸序列编码的分离的多肽; 包含多核苷酸的重组基因构建体; 包含重组基因构建体的转化体和转基因真菌，具有增强的木质素降解酶的产生。进一步公开的是具有寡聚酶活性的多肽，例如在生物质的糖化过程中将顽固性可溶性寡聚酶转化成可发酵糖的酶。

5. 发明公开

EP2885406A4 CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 有权
标题翻译：纤维素 - 发光/光合细胞藻类化合物大肠杆菌
公开(公告)号：EP2885406A4
公开(公告)日：2016-04-27
申请号：EP13829732
申请日：2013-08-15
申请人： BANGLADESH JUTE RES INST
发明人： ALAM MAQSUDUL , ISLAM MOHAMMED SHAHIDUL , HOSSEN MOHAMMED MOSADDEQUE , HAQUE MOHAMMED SAMIUL , ALAM MOHAMMED MONJURUL
IPC分类号： C12N9/42 , C12N15/00
CPC分类号： C12N9/2402 , C12N9/2408 , C12N9/2428 , C12N9/2437 , C12N9/244 , C12N9/2445 , C12N9/2471 , C12N9/2482 , C12N9/2494 , C12N9/88 , C12Y302/01037 , C12Y302/01051 , C12Y302/01055 , C12Y302/01059 , C12Y302/01075 , C12Y302/01089 , C12Y302/0113 , C12Y302/01177
摘要： The present invention provides nucleotide sequences of Macrophomina phaseolina (“M. phaseolina”) that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a β-glucosidase, a a-glucosidase, a xylanase, a mannanse, a β-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a-fucosidases, a β-galactanase, an unsaturated β-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts. These enzymes are also useful for complete hydrolysis of lignocellulosic biomass into simple sugar that can then be fermented to liquid fuels and chemical feedstocks.
摘要翻译：本发明提供了编码具有纤维素分解活性的蛋白质/酶的Macrophomina phaseolina（“M. phaseolina”）的核苷酸序列，包括纤维素酶活性，内切葡聚糖酶，纤维二糖水解酶，α-葡糖苷酶，α-葡糖苷酶，木聚糖酶，甘露聚糖，α-木糖苷酶，α-木糖苷酶，半乳糖苷酶，阿拉伯呋喃糖苷酶，α-岩藻糖苷酶，β-半乳糖苷酶，不饱和的 - 葡萄糖醛酸水解酶和/或寡聚酶活性。还提供了载体，表达构建体和包含酶基因的核苷酸序列的和/或由其组成的宿主细胞。本发明还提供了用于生产酶的方法和用于修饰酶的方法，以便改善其期望的特性。本发明的酶可以多种但不限于制药，农业，食品和饲料加工，生物燃料，能源效率和工业环境。这些酶也可用于将木质纤维素生物质完全水解成简单的糖，然后将其发酵成液体燃料和化学原料。

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