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1. 发明公开

EP1995313A4 NOVEL PHOSPHOLIPASE C 有权转让
标题翻译：新的磷脂酶C
公开(公告)号：EP1995313A4
公开(公告)日：2010-04-07
申请号：EP06728878
申请日：2006-03-10
申请人： MITSUBISHI KAGAKU FOODS CORP
发明人： NAGASAKI EIKO , FUKAZAWA TETSUYA , ONO YASUNORI
IPC分类号： C12N9/20 , C07K1/14 , C12P1/02
CPC分类号： C12N9/20 , C12N9/16 , C12P7/6418 , C12P7/6481 , C12R1/69

2. 发明公开

EP1995313A1 NOVEL PHOSPHOLIPASE C 有权转让
标题翻译： NEUE PHOSPHOLIPASE C
公开(公告)号：EP1995313A1
公开(公告)日：2008-11-26
申请号：EP06728878.7
申请日：2006-03-10
申请人： Mitsubishi-Kagaku Foods Corporation
发明人： NAGASAKI, Eiko, c/o Mitsubishi-Kagaku Foods Corporation , FUKAZAWA, Tetsuya, c/o Mitsubishi-Kagaku Foods Corporation , ONO, Yasunori, c/o Daiichi Sankyo Co. ,Ltd.
IPC分类号： C12N9/20 , C07K1/14
CPC分类号： C12N9/20 , C12N9/16 , C12P7/6418 , C12P7/6481 , C12R1/69
摘要： The present invention provides a phospholipase C enzyme(s) having ability to hydrolyze phospholipid in both acidic and around neutral ranges and the activity in a citrate buffer solution as well as having some degree of heat stability, and having a property not to hydrolyze phosphate esters not containing lipid moieties. The phospholipase C enzyme(s) shows the activity at from acidic to neutral pH and does not substantially hydrolyze any phosphate esters except for phospholipids.
摘要翻译：本发明提供了具有在酸性和中性范围内水解磷脂的能力的磷脂酶C酶和柠檬酸盐缓冲溶液中的活性以及具有一定程度的热稳定性，并且具有不水解磷酸酯的性质不含脂质部分。磷脂酶C显示出从酸性到中性pH的活性，除磷脂外，基本上不含水解磷酸酯。

3. 发明公开

EP3375869A1 MUTANTS OF UNSPECIFIC PEROXYGENASE WITH HIGH MONOOXYGENASE ACTIVITY AND USES THEREOF 有权
公开(公告)号：EP3375869A1
公开(公告)日：2018-09-19
申请号：EP16863726.2
申请日：2016-11-14
申请人： Consejo Superior De Investigaciones Científicas (CSIC)
发明人： MOLINA ESPEJA, Patricia , PLOUGASCA, Francisco José , ALCALDE GALEOTE, Miguel , GÓMEZ DE SANTOS, Patricia
IPC分类号： C12N9/08
CPC分类号： C12N9/0065 , C12N15/80 , C12P7/02 , C12P7/22 , C12P7/26 , C12R1/685 , C12R1/69 , C12Y111/02001
摘要： The invention relates to an unspecific peroxygenase of the Agrocybe aegerita fungus, obtained by means of directed molecular evolution to facilitate the functional expression thereof in an active, soluble and stable form. The peroxygenase described in the invention shows a significant improvement in the functional expression thereof, improved monooxygenase activity and reduced peroxidase activity, in relation to the monooxygenase and peroxidase activities showed by the unspecific wild-type peroxygenase of A. aegerita. The peroxygenase of the invention is useful in chemical processes, including industrial transformations such as the selective oxyfunctionalisation of carbon-hydrogen bonds of various organic compounds.

4. 发明授权

EP1995313B1 NOVEL PHOSPHOLIPASE C 有权转让
标题翻译：新的磷脂酶C
公开(公告)号：EP1995313B1
公开(公告)日：2013-05-08
申请号：EP06728878.7
申请日：2006-03-10
申请人： Mitsubishi-Kagaku Foods Corporation
发明人： NAGASAKI, Eiko, c/o Mitsubishi-Kagaku Foods Corporation , FUKAZAWA, Tetsuya, c/o Mitsubishi-Kagaku Foods Corporation , ONO, Yasunori, c/o Daiichi Sankyo Co. ,Ltd.
IPC分类号： C12N9/20 , C07K1/14 , C12P1/02
CPC分类号： C12N9/20 , C12N9/16 , C12P7/6418 , C12P7/6481 , C12R1/69

5. 发明公开

EP2022850A4 FLAVIN ADENINE DINUCLEOTIDE-BINDING GLUCOSE DEHYDROGENASE 有权
标题翻译：黄素 - 腺苷二酮糖结合葡萄糖 - 脱氢酶
公开(公告)号：EP2022850A4
公开(公告)日：2009-11-18
申请号：EP07744128
申请日：2007-05-25
申请人： AMANO ENZYME INC
发明人： TANAKA NORIAKI , YUUKI KENSUKE
IPC分类号： C12N9/04 , A61K47/48 , C12N1/14 , C12Q1/32 , G01N21/75
CPC分类号： C12R1/69 , C12N9/0006 , C12Q1/54

6. 发明公开

EP2022850A1 FLAVIN ADENINE DINUCLEOTIDE-BINDING GLUCOSE DEHYDROGENASE 有权
标题翻译： FLAVIN-ADENIN-DINUKLEOTID BINDENDE GLUCOSE-DEHYDROGENASE
公开(公告)号：EP2022850A1
公开(公告)日：2009-02-11
申请号：EP07744128.5
申请日：2007-05-25
申请人： Amano Enzyme Inc.
发明人： TANAKA, Noriaki , YUUKI, Kensuke
IPC分类号： C12N9/04 , C12N1/14 , C12Q1/32 , C12R1/69 , G01N21/75
CPC分类号： C12R1/69 , C12N9/0006 , C12Q1/54
摘要： The object is to provide a novel enzyme which enables to determine the glucose level more accurately, a bacterium capable of producing the enzyme, and use of the enzyme. Disclosed is a flavin adenine dinucleotide-binding glucose dehydrogenase having the following properties (1) to (3): (1) the enzyme has an activity of catalyzing a reaction for oxidizing a hydroxyl group in glucose in the presence of an electron acceptor to produce glucono-δ-lactone; (2) the enzyme has a molecular weight of about 100 kDa as measured by SDS-polyacrylamide gel electrophoresis or about 400 kDa as measured by gel filtration chromatography; and (3) the enzyme is less reactive to maltose, D-fructose, D-mannose and D-galactose. Also disclosed is a microorganism Aspergillus oryzae which can produce the enzyme. Further disclosed are a glucose determination method, a reagent for the determination of glucose, and a kit for the determination of glucose, each utilizing the enzyme.
摘要翻译：本发明的目的是提供一种新的酶，其能够更精确地测定葡萄糖水平，能够产生酶的细菌和使用该酶。公开了具有以下性质（1）至（3）的黄素腺嘌呤二核苷酸结合葡萄糖脱氢酶：（1）酶在电子受体存在下具有催化葡萄糖氧化反应的反应活性以产生葡糖酸'内酯; （2）通过SDS-聚丙烯酰胺凝胶电泳测定的酶的分子量为约100kDa，或通过凝胶过滤色谱测定的约400kDa的酶; 和（3）酶对麦芽糖，D-果糖，D-甘露糖和D-半乳糖的反应性较差。还公开了可以产生酶的微生物米曲霉。进一步公开了葡萄糖测定方法，用于测定葡萄糖的试剂和用于测定葡萄糖的试剂盒，各自使用酶。

7. 发明公开

EP3211076A4 NOVEL ALPHA-GLUCOSIDASE 审中-公开
公开(公告)号：EP3211076A4
公开(公告)日：2018-03-21
申请号：EP14904623
申请日：2014-10-20
申请人： SHOWA SANGYO CO
发明人： KAWANO ATSUSHI , YASUTAKE NOZOMU , TERADA ATSUSHI , MATSUMOTO YUJI , TOMINAGA AKIHIRO
IPC分类号： C12N9/24 , C12R1/66 , C12R1/69
CPC分类号： C12N9/24 , C12R1/66 , C12R1/69
摘要： The problem to be solved by the invention is to provide a novel ±-glucosidase exhibiting catalyzing the formation of saccharides containing repeating ±-1,6 linkages and that has a high thermal stability. ±-Glucosidase was discovered in one type of genus Aspergillus , specifically Aspergillus sojae. This ±-glucosidase was found to act on various ±-oligosaccharides and ±-polysaccharides and to exhibit high activity of generating saccharide containing repeating ±-1,6 linkages. Furthermore, this ±-glucosidase was found to have a high thermal stability in which at least 90% of the activity remains after incubation of the enzyme at 50°C for 1 h.

8. 发明授权

EP2368978B1 Novel phospholipase C enzyme(s) 有权转让
标题翻译：新的磷脂酶C
公开(公告)号：EP2368978B1
公开(公告)日：2014-04-30
申请号：EP11164069.4
申请日：2006-03-10
申请人： Mitsubishi-Kagaku Foods Corporation
发明人： Nagasaki, Eiko , Fukazawa, Tetsuya , Ono, Yasunori
IPC分类号： C12N9/20 , C07K1/14 , C12P1/02 , C12N9/16 , C12P7/64 , C12R1/69
CPC分类号： C12N9/20 , C12N9/16 , C12P7/6418 , C12P7/6481 , C12R1/69

9. 发明公开

EP2368978A1 Novel phospholipase C enzyme(s) 有权转让
标题翻译：新型磷脂酶-C酶
公开(公告)号：EP2368978A1
公开(公告)日：2011-09-28
申请号：EP11164069.4
申请日：2006-03-10
申请人： Mitsubishi-Kagaku Foods Corporation
发明人： Nagasaki, Eiko , Fukuzawa, Tetsuya , Ono, Yasunori
IPC分类号： C12N9/20 , C07K1/14 , C12P1/02 , C12N9/16 , C12P7/64 , C12R1/69
CPC分类号： C12N9/20 , C12N9/16 , C12P7/6418 , C12P7/6481 , C12R1/69
摘要： The present invention provides a phospholipase C enzyme(s) having ability to hydrolyze phospholipid in both acidic and around neutral ranges and the activity in a citrate buffer solution as well as having some degree of heat stability, and having a property not to hydrolyze phosphate esters not containing lipid moieties. The phospholipase C enzyme(s) shows the activity at from acidic to neutral pH and does not substantially hydrolyze any phosphate esters except for phospholipids.
摘要翻译：本发明提供了具有在酸性和中性范围内水解磷脂的能力的磷脂酶C酶和柠檬酸盐缓冲溶液中的活性以及具有一定程度的热稳定性，并且具有不水解磷酸酯的性质不含脂质部分。磷脂酶C显示出从酸性到中性pH的活性，除磷脂外，基本上不含水解磷酸酯。

10. 发明授权

EP2022850B1 FLAVIN ADENINE DINUCLEOTIDE-BINDING GLUCOSE DEHYDROGENASE 有权
标题翻译：黄素腺嘌呤二核苷酸结合型葡萄糖脱氢酶
公开(公告)号：EP2022850B1
公开(公告)日：2011-08-31
申请号：EP07744128.5
申请日：2007-05-25
申请人： Amano Enzyme Inc.
发明人： TANAKA, Noriaki , YUUKI, Kensuke
IPC分类号： C12N9/04 , C12N1/14 , C12Q1/32 , A61K47/48 , G01N21/75
CPC分类号： C12R1/69 , C12N9/0006 , C12Q1/54
摘要： The object is to provide a novel enzyme which enables to determine the glucose level more accurately, a bacterium capable of producing the enzyme, and use of the enzyme. Disclosed is a flavin adenine dinucleotide-binding glucose dehydrogenase having the following properties (1) to (3): (1) the enzyme has an activity of catalyzing a reaction for oxidizing a hydroxyl group in glucose in the presence of an electron acceptor to produce glucono-δ-lactone; (2) the enzyme has a molecular weight of about 100 kDa as measured by SDS-polyacrylamide gel electrophoresis or about 400 kDa as measured by gel filtration chromatography; and (3) the enzyme is less reactive to maltose, D-fructose, D-mannose and D-galactose. Also disclosed is a microorganism Aspergillus oryzae which can produce the enzyme. Further disclosed are a glucose determination method, a reagent for the determination of glucose, and a kit for the determination of glucose, each utilizing the enzyme.

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