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1. 发明公开

EP4169931A1 NUCLEIC ACID LIGAND AND USE THEREOF 审中-实审
公开(公告)号：EP4169931A1
公开(公告)日：2023-04-26
申请号：EP21828968.4
申请日：2021-06-21
申请人： Nuhigh Biotechnologies Co., Ltd
发明人： BI, Wanli , HE, Wenlong , FU, Dongke , PAN, Jie , XING, Yadong , WANG, Zhiqing , QIN, Pingping
IPC分类号： C07H21/00 , C12N9/99 , C12P19/34
摘要： Provided are a nucleic acid ligand (nucleic acid polymerase substrate analog), a mixture thereof, and the use thereof. The mixture of the nucleic acid polymerase substrate analog contains two or more nucleic acid polymerase substrate analogs. The nucleic acid polymerase substrate analog is a single nucleic acid molecule or nucleic acid molecule analog which forms complementary pairing within a molecule, or a single or two nucleic acid molecules or nucleic acid molecule analogs which form complementary pairing between molecules; and a structure formed thereby has the characteristics of a nucleic acid polymerase substrate. When an amplification reaction mixture is at or below a certain temperature, the enzyme activity of a nucleic acid polymerase is inhibited by means of the nucleic acid ligand and there is no residual enzyme activity. When the reaction mixture is heated, the nucleic acid polymerase is separated from the nucleic acid polymerase substrate analog to exert activity and form a primer extension product, thereby achieving the effect of inhibiting non-specific amplification. The nucleic acid polymerase substrate analog is suitable for all polymerases and can be widely used in the field of nucleic acid amplification. The 3' end of the nucleic acid ligand has a modification which inhibits the extension thereof.

2. 发明公开

EP3283630A1 OLIGONUCLEOTIDE FRAGMENT, AND METHOD AS WELL AS APPLICATION OF SELECTIVE AMPLIFICTION OF VARIANT OF TARGET NUCLEIC ACID SEQUENCE USING THE SAME 有权
标题翻译：寡核苷酸片段和方法以及使用相同核酸序列变体的选择性扩增的应用
公开(公告)号：EP3283630A1
公开(公告)日：2018-02-21
申请号：EP15888914.7
申请日：2015-06-18
申请人： Nuhigh Biotechnologies Co., Ltd
发明人： BI, Wanli , WANG, Zhifeng , XU, Xin
IPC分类号： C12N15/11 , C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6853 , C12Q2525/186 , C12Q2525/301 , C12Q2525/107 , C12Q2525/125 , C12Q2525/113 , C12Q2525/117
摘要： Provided is a method for selective amplification of a variant of a target nucleic acid sequence. Also provided is an oligonucleotide fragment with a particular structure which is used in the the method, the oligonucleotide fragment includes a primer region and a variant recognition region.

3. 发明公开

EP4163371A1 NUCLEIC ACID EXTRACTION METHOD AND APPLICATION 审中-实审
公开(公告)号：EP4163371A1
公开(公告)日：2023-04-12
申请号：EP21818014.9
申请日：2021-05-31
申请人： Nuhigh Biotechnologies Co., Ltd
发明人： FU, Dongke , HE, Wenlong , LIU, Liandi , BI, Wanli
IPC分类号： C12N15/10
摘要： Disclosed is a nucleic acid extraction method. By using a quaternary ammonium salt, nucleic acid molecules in a solution are adsorbed to adsorbing materials such as silicon beads, magnetic beads, diatomite or silica gel or precipitated, thereby improving efficiency of nucleic acid purification and recovery.

4. 发明公开

EP3348642A1 MOLECULAR WEIGHT INTERNAL STANDARD AND APPLICATIONS THEREOF 审中-公开
公开(公告)号：EP3348642A1
公开(公告)日：2018-07-18
申请号：EP16838600.1
申请日：2016-08-26
申请人： Nuhigh Biotechnologies Co., Ltd
发明人： BI, Wanli , LU, Weihua
IPC分类号： C12N15/11 , C12Q1/68
CPC分类号： C12Q1/6876 , C12N15/11 , C12Q1/68 , C12Q2600/166
摘要： Provided is a molecular weight internal standard, consisting of n nucleotide fragments that have different molecular weights and identifiable markers, the n nucleotide fragments comprising at least p nucleotide fragment pairs differing by one base, and one fragment of the nucleotide fragment pair differing by one base having m bases, and the other fragment having m+1 or m-1 bases; p is 1 to n/2 when n is an even number and p is 1 to (n-1)/2 when n is an odd number. Compared with current commercial molecular weight internal standards, the molecular weight internal standard can be used to identify a single base and differentiate PCR product fragments differing by one base, and is applicable to STR analysis, sequencing, large fragment analysis, and genome analysis.

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