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1. 发明公开

EP0791071A1 ASSAY FOR PROLINE IMINOPEPTIDASE AND OTHER HYDROLYTIC ACTIVITIES 失效
标题翻译：分析法的脯氨酸亚氨基等活动水解
公开(公告)号：EP0791071A1
公开(公告)日：1997-08-27
申请号：EP95939592.0
申请日：1995-10-25
申请人： LITMUS CONCEPTS, INC.
发明人： LAWRENCE, Paul, J. , ANDREASEN, Terrence, J. , SHOCKEY, David, R.
IPC分类号： C12Q1
CPC分类号： C12Q1/37 , C12Q1/34 , C12Q2334/70 , C12Q2337/30
摘要： The presence of an enzymatically active hydrolase in a fluid sample is detected by contacting the sample with a solid-phase conjugate which is susceptible to cleavage by the hydrolase, and simultaneously or shortly thereafter, contacting the sample with an indicator which undergoes a detectable change upon the action of a reporter group. The reporter group is part of the conjugate and is liberated from it either partly or entirely by the action of the hydrolase. The indicator is susceptible to action by the reporter group only upon decoupling of the reporter group from the remainder of the conjugate, the decoupling occuring either in part or entirely upon action of the hydrolase. Also provided by this invention are various forms of a dry, self-contained test device which contains the conjugate described above plus the indicator and all other reagents and components necessary to achieve a detectable indication of the presence or absence of a catalytically active hydrolase. Preferred embodiments of the device also contain positive and negative controls.

2. 发明公开

EP0380932A3 SOLID PHASE TEST FOR THE DETECTION OF PERIODONTAL DISEASE IN SUBGINGIVAL PLAQUE 失效
标题翻译：用于检测子宫肌瘤中的周围疾病的固相试验
公开(公告)号：EP0380932A3
公开(公告)日：1991-11-06
申请号：EP90100409
申请日：1990-01-10
申请人： ORAL B LABORATORIES A DIVISION OF GILLETTE CANADA INCORPORATED
发明人： KERSCHENSTEINER, DANIEL
IPC分类号： G01N33/50 , A61B10/00 , A61C19/04 , C12Q1/00 , C12Q1/04 , C12Q1/37 , C12R1/01
CPC分类号： C12Q1/04 , C12Q1/37 , C12Q2334/70 , C12Q2337/30 , G01N2333/976
摘要： Subgingival plaque samples can be analyzed for the presence of the trypsin substrate N-benzoyl-DL-arginine-2-naphthylamide (BANA) in order to make a clinical diagnosis of periodontal disease. The solid phase test utilizes enzyme sensitive reagents adsorbed separately onto two elements. The sample is added to one element, the second element contacted therewith and water is added to initiate the reaction. The resulting analysis is complete within thirty minutes. Positive tests indicating periodontal disease-causing organisms are determined by a color change on the element containing the Fast Black K salt color developer. The test provides a stable, readable, permanent record which can be included in the patient's dental records.

3. 发明公开

EP0255341A3 Reagent for testing periodontal diseases 失效
标题翻译：用于测试周围疾病的试剂
公开(公告)号：EP0255341A3
公开(公告)日：1990-01-31
申请号：EP87306663.3
申请日：1987-07-28
申请人： SUNSTAR KABUSHIKI KAISHA
发明人： Tanaka, Toshiyuki , Nakamura, Masakazu , Suido, Hirohisa
IPC分类号： C12Q1/37 , C07K5/00 , C07K7/00
CPC分类号： C12Q1/37 , C12Q2337/12 , C12Q2337/30 , Y10S530/802
摘要： A reagent for testing periodontal diseases which diagnoses or prognosticates contraction or progress of said diseases by determining aminopeptidase-like enzymatic activity in a specimen, said reagent comprises as a substrate for the enzyme either or both compounds of the formula:
X-Z-Arg-Y [1]
wherein Arg is arginine residue; X is hydrogen or an amino blocking group; Y is a color developing group attached to the C-terminal of Arg; and Z is an amino acid or peptide residue composed of 1 to 4 amino acids or their blocked derivatives, the C-terminal of which is attached to the N-terminal of Arg, and
Xʹ-Zʹ-Pro-Yʹ [2]
wherein Pro is proline residue; Xʹ is hydrogen or an amino blocking group; Yʹ is a color developing group attached to the C-terminal of Pro; and Zʹ is an amino acid or peptide residue composed of 0 to 4 amino acids or their blocked derivatives, the C-terminal of which is attached to the N-terminal of Pro.

4. 发明公开

EP0175421A2 Test method for the presumptive identification of streptococci 失效
标题翻译： Testverfahren zur vermutlichen Identifizierung von Streptokokken。
公开(公告)号：EP0175421A2
公开(公告)日：1986-03-26
申请号：EP85201467.9
申请日：1985-09-16
申请人： WARNER-LAMBERT COMPANY
发明人： Buenviaje Wantland, Nora M. , Seo, John S.
IPC分类号： C12Q1/14 , C12Q1/00
CPC分类号： C12Q1/14 , C12Q2334/10 , C12Q2337/30 , G01N2333/315
摘要： The identification of streptococci can be carried out simply and quickly using a series of tests and comparison of the test results with an identification scheme.
摘要翻译：可以通过一系列测试和测试结果与识别方案的比较，简单快速地进行链球菌的鉴定。

5. 发明公开

EP0122028A1 Colorimetric assay for enzymes, diagnostic article therefor and a method for forming such article 失效
标题翻译：比色测定适合于该诊断装置以及用于制造这种装置的方法的酶。
公开(公告)号：EP0122028A1
公开(公告)日：1984-10-17
申请号：EP84301525.6
申请日：1984-03-07
申请人： E-Y LABORATORIES, INC.
发明人： Chun, Peter Kwok Chi , Chu, Albert En-Yuen
IPC分类号： C12Q1/00 , C12Q1/04 , C12Q1/36
CPC分类号： C12Q1/00 , C12Q1/04 , C12Q1/37 , C12Q2334/10 , C12Q2334/52 , C12Q2334/70 , C12Q2337/30 , G01N2333/22 , G01N2333/24
摘要： A method for the colorimetric assay of a suspected enzyme-containing substance in a biologically derived specimen is provided by absorbing a substrate for the enzyme onto an absorbent surface such as a swab, which is contacted with the specimen to produce a color if the enzyme is present. Multiple substrates producing different colors may be absorbed onto the swab for the detection of multiple enzymes. The invention includes a diagnostic article, and its formation, incorporating this principle.
摘要翻译：如拭子，所有这一切都与样品接触，以产生彩色如果酶是：一种用于在生物衍生试样一个怀疑含有酶的物质的比色测定方法，通过吸收所述酶的底物走上到吸收表面提供目前。多基片产生不同颜色可以走上拭子用于检测多种酶的吸收。本发明包括一种诊断物品，和它的形成，结合这一原理。

6. 发明授权

EP1235927B1 NOUVEAUX SUBSTRATS ENZYMATIQUES POUR LA DETECTION DE PSEUDOMONAS AERUGINOSA 有权
标题翻译：新的酶底物铜绿假单胞菌的检测
公开(公告)号：EP1235927B1
公开(公告)日：2006-03-08
申请号：EP00985413.4
申请日：2000-12-06
申请人： Biomerieux S.A.
发明人： DESMONCEAUX, Mireille , MONGET, Daniel
IPC分类号： C12Q1/04 , C12Q1/37 , C07C237/20
CPC分类号： C12Q1/04 , C07C237/04 , C07D209/40 , C07D311/16 , C12Q1/37 , C12Q2326/90 , C12Q2337/10 , C12Q2337/12 , C12Q2337/22 , C12Q2337/30
摘要： The invention concerns an enzymatic substrate consisting of a target portion and a marker portion, the target portion being separated from the marker portion by hydrolysing the substrate, said target portion characterising the desired enzymatic activity. The invention also concerns a composition containing at least such a substrate and a method for detecting the Pseudomonas aeruginosa bacterial species. The target portion of the substrate enables to reveal a beta -alanine aminopeptidase enzymatic activity and the marker portion is a molecule revealing the hydrolysis reaction. The invention is particularly useful in the field of diagnosis.

7. 发明公开

EP1235927A2 NOUVEAUX SUBSTRATS ENZYMATIQUES POUR LA DETECTION DE PSEUDOMONAS AERUGINOSA 有权
标题翻译：新的酶底物铜绿假单胞菌的检测
公开(公告)号：EP1235927A2
公开(公告)日：2002-09-04
申请号：EP00985413.4
申请日：2000-12-06
申请人： Biomerieux S.A.
发明人： DESMONCEAUX, Mireille , MONGET, Daniel
IPC分类号： C12Q1/04 , C12Q1/37 , C07C237/20
CPC分类号： C12Q1/04 , C07C237/04 , C07D209/40 , C07D311/16 , C12Q1/37 , C12Q2326/90 , C12Q2337/10 , C12Q2337/12 , C12Q2337/22 , C12Q2337/30
摘要： The invention concerns an enzymatic substrate consisting of a target portion and a marker portion, the target portion being separated from the marker portion by hydrolysing the substrate, said target portion characterising the desired enzymatic activity. The invention also concerns a composition containing at least such a substrate and a method for detecting thePseudomonas aeruginosabacterial species. The target portion of the substrate enables to reveal a β-alanine aminopeptidase enzymatic activity and the marker portion is a molecule revealing the hydrolysis reaction. The invention is particularly useful in the field of diagnosis.

8. 发明授权

EP0791071B1 ASSAY FOR PROLINE IMINOPEPTIDASE AND OTHER HYDROLYTIC ACTIVITIES 失效
标题翻译：分析法的脯氨酸亚氨基等活动水解
公开(公告)号：EP0791071B1
公开(公告)日：1999-09-15
申请号：EP95939592.2
申请日：1995-10-25
申请人： LITMUS CONCEPTS, INC.
发明人： LAWRENCE, Paul, J. , ANDREASEN, Terrence, J. , SHOCKEY, David, R.
IPC分类号： C12Q1/37 , G01N33/52
CPC分类号： C12Q1/37 , C12Q1/34 , C12Q2334/70 , C12Q2337/30
摘要： The presence of an enzymatically active hydrolase in a fluid sample is detected by contacting the sample with a solid-phase conjugate which is susceptible to cleavage by the hydrolase, and simultaneously or shortly thereafter, contacting the sample with an indicator which undergoes a detectable change upon the action of a reporter group. The reporter group is part of the conjugate and is liberated from it either partly or entirely by the action of the hydrolase. The indicator is susceptible to action by the reporter group only upon decoupling of the reporter group from the remainder of the conjugate, the decoupling occuring either in part or entirely upon action of the hydrolase. Also provided by this invention are various forms of a dry, self-contained test device which contains the conjugate described above plus the indicator and all other reagents and components necessary to achieve a detectable indication of the presence or absence of a catalytically active hydrolase. Preferred embodiments of the device also contain positive and negative controls.

9. 发明公开

EP0292708A3 Detection of Bacteroides gingivalis 失效
标题翻译：检测菌株GINGIVALIS
公开(公告)号：EP0292708A3
公开(公告)日：1990-06-27
申请号：EP88106533.8
申请日：1988-04-23
申请人： THE RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YORK
发明人： Zambon, Joseph J. , Genco, Robert J.
IPC分类号： C12Q1/04 , C12Q1/36
CPC分类号： C12Q1/04 , C12Q2337/30 , Y10S435/822 , Y10S530/825
摘要： The present invention relates to the detection of B. gingivalis by the specific ability of B. gingivalis to hydrolyze N-carbobenzoxy-glycyl-glycyl-L-arginine-B-napthylamide derivatives. The present invention also relates to the use of assay systems which inhibit serum amino peptidase and enhance the detection of the B. gingivalis N-CBz-Gly-Gly-Arg peptidase. The assay system inhibits the serum enzyme by the use of a serum aminopeptidase inhibitor which inhibits the activity of serum aminopeptidase to a greater extent than it inhibits the activity of B. gingivalis N-CBz-Gly-Gly-Arg peptidase. The assay system also increases the reliability of detection of the B. gingivalis enzyme by the use of enhancing materials which enhance the enzyme activity of B. gingivalis N-CBz-Gly-Gly-Arg peptidase to a greater extent than such materials enhance the enzyme activity of serum aminopeptidase.

10. 发明授权

EP0165905B1 Diagnostisches Testverfahren zum Nachweis von oralen pathogenen Bakteriengemischen 失效
标题翻译：用于检测口腔致病菌细菌混合物的诊断测定方法
公开(公告)号：EP0165905B1
公开(公告)日：1989-03-08
申请号：EP85810281.7
申请日：1985-06-18
申请人： THE BOARD OF REGENTS OF THE UNIVERSITY OF MICHIGAN
发明人： Lang, Niklaus P., Dr. med. dent. c/o Zahnmed. , Gusberti, Francesco A., Dr. med. dent. c/o Zahn- , Syed, Salam A. c/o Zahnmed. Kliniken der , Loesche Walter J
IPC分类号： C12Q1/04 , C12Q1/36
CPC分类号： C12Q1/37 , C12Q1/04 , C12Q2337/30
摘要： 1. Diagnostic procedure for detecting a mixture of pathogenic oral plaque bacteria associated with gingivitis and parodontitis, characterized in that an oral plaque specimen is collected in a sterile, small glass bottle containing 0.5 - 1 ml of HEPES buffer or reduced transport liquid and from 5 - 10 cleaned, sterile glass beads having a diameter of from 2 - 3 mm, and that the small bottle is thoroughly shaken for about 10 sec., the contents are divided up into from 4 - 8 parts of 100 mu l each, and each part is tested for enzymatic activity by mixing each part specimen with a chromogenic substrate and incubating it, the substrates being chosen from the group of the following substrates : N-alpha-benzoyl-D,L-arginine-beta-naphthylamide.HCl ; L-valine-beta-naphthylamide ; L-leucine-beta-naphthylamide.HCl ; L-pyrrolidonyl-beta-naphthylamide ; L-serine-beta-naphthylamide ; L-arginine-beta-naphthylamide ; L-alamine-beta-naphthylamide ; L-phenylalanine-beta-naphthylamide ; L-hydroxy-proline-beta-naphthylamide and glycylglycyl-beta-naphthylamide, which are substrates of enzymes that are released by pathogenic bacteria contained in the mixture to be detected, the positive enzymatic reaction being detected by means of attacked substrates which, through the addition of chromogenic reagents, have modified light-absorption values, and the substantially positive enzymatic reaction indicates the presence of a pathogenic bacterial mixture.
摘要翻译： 1.用于检测与牙龈炎和牙菌斑相关的病原性口腔斑块细菌的混合物的诊断程序，其特征在于将口腔斑块样品收集在含有0.5-1ml HEPES缓冲液或还原的运输液体的无菌小玻璃瓶中，并从5 - 10个直径为2-3mm的清洁无菌玻璃珠，并将小瓶充分摇动约10秒，将内容物分成4至8份，每份100μl，每份通过将各部分样品与显色底物混合并将其孵育来测试部分酶活性，底物选自下列底物：N-α-苯甲酰-D，L-精氨酸-β-萘酰胺。 L-缬氨酸-β-萘酰胺; L-亮氨酸-β-萘酰胺。 L-吡咯烷酮基-β-萘酰胺; L-丝氨酸-β-萘酰胺; L-精氨酸-β-萘酰胺; L-阿拉伯-β-萘酰胺; L-苯丙氨酸-β-萘酰胺; L-羟基 - 脯氨酸-β-萘基酰胺和甘氨酰甘氨酰基-β-萘基酰胺，它们是待检测的混合物中包含的病原体释放的酶的底物，通过侵袭的底物检测到阳性酶反应，显色剂的添加具有改变的光吸收值，并且基本上正的酶反应表明存在病原菌混合物。

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