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1. 发明授权

EP0694077B1 REPORTER ENZYME RELEASE TECHNOLOGY: METHODS OF ASSAYING FOR THE PRESENCE OF ASPARTIC PROTEASES AND OTHER HYDROLYTIC ENZYME ACTIVITIES 失效
标题翻译：报道酶释放技术：方法天冬氨酸蛋白酶和水解酶的其他活动有无探测
公开(公告)号：EP0694077B1
公开(公告)日：2000-05-24
申请号：EP94914164.2
申请日：1994-04-13
申请人： LITMUS CONCEPTS, INC.
发明人： LAWRENCE, Paul, J. , CHAUHURI, Aulena , ANDREASEN, Terrence, J.
IPC分类号： C12Q1/34 , C12Q1/37
CPC分类号： C12N11/02 , C12M21/18 , C12M41/46 , C12N11/06 , C12Q1/37 , G01N2333/4731 , G01N2333/805
摘要： Methods of assaying for the presence of enzymatically active hydrolases (i.e., hydrolytic enzymes) in a sample or specimen are disclosed. In particular, a method of detecting candidiasis by assaying for the presence of enzymatically active aspartic protease in a sample is provided. In these methods, a sample or specimen is contacted with a solid support. The solid support with which the sample is contacted has a reporter enzyme (i.e., a signal generating enzyme) immobilized thereon. The reporter enzyme is immobilized on the solid support in a manner such that it is released from the solid support upon action of the enzymatically active hydrolase if the enzymatically active hydrolase is, in fact, present in the sample. The sample after having been contacted with the solid support is combined with an indicator. The indicator is any chemical species which is susceptible to a detectable change, usually a change in color, upon action of the reporter enzyme. A detectable change in the indicator is an indication that the enzymatically active hydrolase is present in the sample. Moreover, the preence of an enzymatically active hydrolase in a sample may indicate the presence of a particular pathogen or disease state such as, for example, candidiasis. In addition to the methods of assaying for the presence of enzymatically active aspartic protease and other hydrolytic enzymes, a dry, self-contained test device for assaying for the presence of an enzymatically active hydrolase in a sample is disclosed. In particular, a dry, self-contained test device for testing a sample for the presence of candidiasis by assaying for the presence of enzymatically active aspartic protease is also disclosed. These test devices combine a reporter enzyme immobilized on a solid support, an indicator, and all other reagents and components necessary to achieve a detectable indication of the presence or absence of the enzymatically active hydrolase whose presence is being detected in the sample, and preferred embodiments contain positive and negative controls as well.

2. 发明授权

EP0925501B1 pH AND AMINE TEST ELEMENTS AND APPLICATIONS TO DIAGNOSIS OF VAGINAL INFECTIONS 失效
标题翻译： PH和AMIN-TEST元素及其用途阴道感染的诊断
公开(公告)号：EP0925501B1
公开(公告)日：2009-06-17
申请号：EP97933328.3
申请日：1997-07-10
申请人： LITMUS CONCEPTS, INC.
发明人： LAWRENCE, Paul, J. , LY, Peter, U. , SHOCKEY, David, R.
IPC分类号： G01N31/22 , G01N33/52 , G01N33/84
CPC分类号： G01N31/221 , G01N33/52 , G01N33/84 , Y10T436/173845
摘要： Tests for elevated pH and volatile amines in aqueous fluids are disclosed, including tests useful in the diagnosis of bacterial vaginosis and in other biological conditions. By using formulated indicators and indicators held in matrices that are permeable to gas but not to liquid, the tests provide clear and sharp transitions detectable by visual or machine-readable means rather than by subjective judgements such as small gradations in color or olfactory determinations. The tests lend themselves readily to iconic readouts of the test indications and to the inclusion of positive and negative controls.

3. 发明公开

EP0925501A1 pH AND AMINE TEST ELEMENTS AND APPLICATIONS TO DIAGNOSIS OF VAGINAL INFECTIONS 失效
标题翻译： PH和AMIN-TEST元素及其用途阴道感染的诊断
公开(公告)号：EP0925501A1
公开(公告)日：1999-06-30
申请号：EP97933328.0
申请日：1997-07-10
申请人： LITMUS CONCEPTS, INC.
发明人： LAWRENCE, Paul, J. , LY, Peter, U. , SHOCKEY, David, R.
IPC分类号： G01N31 , G01N33
CPC分类号： G01N31/221 , G01N33/52 , G01N33/84 , Y10T436/173845
摘要： Tests for elevated pH and volatile amines in aqueous fluids are disclosed, including tests useful in the diagnosis of bacterial vaginosis and in other biological conditions. By using formulated indicators and indicators held in matrices that are permeable to gas but not to liquid, the tests provide clear and sharp transitions detectable by visual or machine-readable means rather than by subjective judgements such as small gradations in color or olfactory determinations. The tests lend themselves readily to iconic readouts of the test indications and to the inclusion of positive and negative controls.

4. 发明公开

EP0341249A4 DEVELOPING SYSTEM AND A METHOD OF USING THE DEVELOPING SYSTEM FOR DETECTION OF CARBOXYLIC ACIDS 失效
标题翻译：开发系统和使用开发系统检测羧酸的方法
公开(公告)号：EP0341249A4
公开(公告)日：1990-12-05
申请号：EP88900528
申请日：1987-12-14
申请人： LITMUS CONCEPTS INC
发明人： PRICE NEIL H , LAWRENCE PAUL J
IPC分类号： G01N33/50 , G01N21/78 , G01N31/00 , G01N31/10 , G01N33/52 , G01N33/00
CPC分类号： G01N33/52 , Y10T436/201666
摘要： A developing system and a method of using the developing system for detecting the presence of carboxylic acids in a specimen. A specimen is contacted with a developing system containing a metal salt reactive with carboxylic acids present in the specimen to form metal-carboxylic acid complexes. Any such complexes are colorimetrically detected by their activity in catalyzing oxidation of a chromogen such as guaiac. The method is useful in detecting intestinal helminth infestation.

5. 发明公开

EP0632892A1 FAST RESPONSE TEST PANEL 失效
标题翻译：快速无试验装置。
公开(公告)号：EP0632892A1
公开(公告)日：1995-01-11
申请号：EP93908289.0
申请日：1993-03-10
申请人： LITMUS CONCEPTS, INC.
发明人： LAWRENCE, Paul, J. , BUCCAFURNI, Marian, B. , LAWRENCE, Leah, P.
IPC分类号： G01N31 , B01L3 , G01N33
CPC分类号： B01L3/5027 , B01L2200/0684 , B01L2300/0627 , B01L2400/0406
摘要： A dry test panel test samples such as undiluted bodily fluid specimens. The panel contains all reagents and components necessary to achieve a visible indication of the presence or absence of a suspect analyte in the sample, and preferred embodiments contain positive and negative controls (36, 37) as well. The device contains an internal chamber (31) into which the specimen is introduced, and which contains all materials necessary for the reactions which produce a color change which is visible on the outer surface of the device. The materials are positioned in the chamber (31) in such a manner that they are activated only when the chamber (31) is filled with the specimen.

6. 发明授权

EP0341249B1 DEVELOPING SYSTEM AND A METHOD OF USING THE DEVELOPING SYSTEM FOR DETECTION OF CARBOXYLIC ACIDS 失效
标题翻译：开发体系和将其用于炭酸的检测方法。
公开(公告)号：EP0341249B1
公开(公告)日：1994-07-13
申请号：EP88900528.6
申请日：1987-12-14
申请人： LITMUS CONCEPTS, INC.
发明人： PRICE, Neil, H. , LAWRENCE, Paul, J.
IPC分类号： G01N33/00 , G01N33/50
CPC分类号： G01N33/52 , Y10T436/201666
摘要： A developing system and a method of using the developing system for detecting the presence of carboxylic acids in a specimen. A specimen is contacted with a developing system containing a metal salt reactive with carboxylic acids present in the specimen to form metal-carboxylic acid complexes. Any such complexes are colorimetrically detected by their activity in catalyzing oxidation of a chromogen such as guaiac. The method is useful in detecting intestinal helminth infestation.

7. 发明授权

EP0632892B1 FAST RESPONSE TEST PANEL 失效
标题翻译：快速无试验装置
公开(公告)号：EP0632892B1
公开(公告)日：2003-01-22
申请号：EP93908289.7
申请日：1993-03-10
申请人： LITMUS CONCEPTS, INC.
发明人： LAWRENCE, Paul, J. , BUCCAFURNI, Marian, B. , LAWRENCE, Leah, P.
IPC分类号： G01N31/22 , G01N33/50 , B01L3/00
CPC分类号： B01L3/5027 , B01L2200/0684 , B01L2300/0627 , B01L2400/0406
摘要： A dry test panel test samples such as undiluted bodily fluid specimens. The panel contains all reagents and components necessary to achieve a visible indication of the presence or absence of a suspect analyte in the sample, and preferred embodiments contain positive and negative controls (36, 37) as well. The device contains an internal chamber (31) into which the specimen is introduced, and which contains all materials necessary for the reactions which produce a color change which is visible on the outer surface of the device. The materials are positioned in the chamber (31) in such a manner that they are activated only when the chamber (31) is filled with the specimen.

8. 发明授权

EP0791071B1 ASSAY FOR PROLINE IMINOPEPTIDASE AND OTHER HYDROLYTIC ACTIVITIES 失效
标题翻译：分析法的脯氨酸亚氨基等活动水解
公开(公告)号：EP0791071B1
公开(公告)日：1999-09-15
申请号：EP95939592.2
申请日：1995-10-25
申请人： LITMUS CONCEPTS, INC.
发明人： LAWRENCE, Paul, J. , ANDREASEN, Terrence, J. , SHOCKEY, David, R.
IPC分类号： C12Q1/37 , G01N33/52
CPC分类号： C12Q1/37 , C12Q1/34 , C12Q2334/70 , C12Q2337/30
摘要： The presence of an enzymatically active hydrolase in a fluid sample is detected by contacting the sample with a solid-phase conjugate which is susceptible to cleavage by the hydrolase, and simultaneously or shortly thereafter, contacting the sample with an indicator which undergoes a detectable change upon the action of a reporter group. The reporter group is part of the conjugate and is liberated from it either partly or entirely by the action of the hydrolase. The indicator is susceptible to action by the reporter group only upon decoupling of the reporter group from the remainder of the conjugate, the decoupling occuring either in part or entirely upon action of the hydrolase. Also provided by this invention are various forms of a dry, self-contained test device which contains the conjugate described above plus the indicator and all other reagents and components necessary to achieve a detectable indication of the presence or absence of a catalytically active hydrolase. Preferred embodiments of the device also contain positive and negative controls.

9. 发明公开

EP0694077A1 REPORTER ENZYME RELEASE TECHNOLOGY: METHODS OF ASSAYING FOR THE PRESENCE OF ASPARTIC PROTEASES AND OTHER HYDROLYTIC ENZYME ACTIVITIES 失效
标题翻译：报道酶FREISETZUNGSTECKNOLOGIE：检测方法的天冬氨酸蛋白酶和酶HYDRLYTISCKEN其他活动的存在
公开(公告)号：EP0694077A1
公开(公告)日：1996-01-31
申请号：EP94914164.0
申请日：1994-04-13
申请人： LITMUS CONCEPTS, INC.
发明人： LAWRENCE, Paul, J. , CHAUHURI, Aulena , ANDREASEN, Terrence, J.
IPC分类号： C12M1 , C12Q1 , C12R1
CPC分类号： C12N11/02 , C12M21/18 , C12M41/46 , C12N11/06 , C12Q1/37 , G01N2333/4731 , G01N2333/805
摘要： Methods of assaying for the presence of enzymatically active hydrolases (i.e., hydrolytic enzymes) in a sample or specimen are disclosed. In particular, a method of detecting candidiasis by assaying for the presence of enzymatically active aspartic protease in a sample is provided. In these methods, a sample or specimen is contacted with a solid support. The solid support with which the sample is contacted has a reporter enzyme (i.e., a signal generating enzyme) immobilized thereon. The reporter enzyme is immobilized on the solid support in a manner such that it is released from the solid support upon action of the enzymatically active hydrolase if the enzymatically active hydrolase is, in fact, present in the sample. The sample after having been contacted with the solid support is combined with an indicator. The indicator is any chemical species which is susceptible to a detectable change, usually a change in color, upon action of the reporter enzyme. A detectable change in the indicator is an indication that the enzymatically active hydrolase is present in the sample. Moreover, the preence of an enzymatically active hydrolase in a sample may indicate the presence of a particular pathogen or disease state such as, for example, candidiasis. In addition to the methods of assaying for the presence of enzymatically active aspartic protease and other hydrolytic enzymes, a dry, self-contained test device for assaying for the presence of an enzymatically active hydrolase in a sample is disclosed. In particular, a dry, self-contained test device for testing a sample for the presence of candidiasis by assaying for the presence of enzymatically active aspartic protease is also disclosed. These test devices combine a reporter enzyme immobilized on a solid support, an indicator, and all other reagents and components necessary to achieve a detectable indication of the presence or absence of the enzymatically active hydrolase whose presence is being detected in the sample, and preferred embodiments contain positive and negative controls as well.

10. 发明公开

EP0391976A4 FECAL OCCULT BLOOD TEST REAGENTS AND METHODS 失效
标题翻译： FECAL成人血液检测试剂和方法
公开(公告)号：EP0391976A4
公开(公告)日：1991-01-30
申请号：EP89901647
申请日：1988-12-19
申请人： LITMUS CONCEPTS, INC.
发明人： LAWRENCE, PAUL, J.
IPC分类号： C12Q1/28 , G01N33/72 , G01N21/78
CPC分类号： G01N33/725 , C12Q1/28 , C12Q2326/30 , Y10S436/904
摘要： An improved method for detecting peroxidatively active materials in a sample is provided, the method incorporating antimalarial drugs such as quine, quinidine, chloroquine, primaquine, and quinacrine as modulating compounds. In a preferred embodiment, the method is a fecal occult blood test (FOBT), in which a fecal specimen is contacted with a chromogen and a hydroperoxide in the presence of a selected quinoline derivative. The method enables careful modulation of test sensitivity and specificity, and further provides an FOBT in which interference from dietary meat ingestion is substantially eliminated.

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