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1. 发明授权

EP2316964B1 Test media and quantitative or qualitative method for identification and differentiation of bacteria in a test sample 有权
标题翻译：测试介质和用于在测试样品中检测和区分细菌之间的定量或定性的方法
公开(公告)号：EP2316964B1
公开(公告)日：2013-09-04
申请号：EP10012276.1
申请日：2005-06-08
申请人： Micrology Laboratories L.L.C.
发明人： Roth, Geoffrey N. , Roth, Jonathan N.
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q1/045 , C12Q1/10 , C12Q2334/00 , C12Q2334/50 , G01N2333/245 , G01N2333/25 , G01N2333/255 , G01N2333/924 , Y02A50/451
摘要： A test medium and method for detecting, quantifying, identifying and differentiating up to four (4) separate biological materials in a test sample. A test medium is disclosed which allows quantifying and differentiating under ambient light aggregates of biological entities producing specific enzymes, which might include general coliforms, E. coli, Aeromonas, and Salmonella in a single test medium. A new class of nonchromogenic substrates is disclosed which produce a substantially black, non-diffusible precipitate. This precipitate is not subject to interference from other chromogenic substrates present in the test medium.; In one embodiment, the substrates are selected such that E. coli colonies present in the test medium show as substantially black, general coliforms colonies show in the test medium as a blue-violet color, Aeromonas colonies present in the test medium show as a generally red-pink color, and Salmonella colonies show as a generally teal-green color. Other microorganisms and color possibilities for detection and quantification thereof are also disclosed. An inhibitor and method for making a test medium incorporating the inhibitor are disclosed.

2. 发明公开

EP2316964A1 Test media and quantitative or qualitative method for identification and differentiation of biological materials in a test sample 有权
标题翻译：测试介质和用于在测试样品中检测和区分细菌之间的定量或定性的方法
公开(公告)号：EP2316964A1
公开(公告)日：2011-05-04
申请号：EP10012276.1
申请日：2005-06-08
申请人： Micrology Laboratories L.L.C.
发明人： Roth, Geoffrey N. , Roth, Jonathan N.
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q1/045 , C12Q1/10 , C12Q2334/00 , C12Q2334/50 , G01N2333/245 , G01N2333/25 , G01N2333/255 , G01N2333/924 , Y02A50/451
摘要： A test medium and method for detecting, quantifying, identifying and differentiating up to four (4) separate biological materials in a test sample. A test medium is disclosed which allows quantifying and differentiating under ambient light aggregates of biological entities producing specific enzymes, which might include general coliforms, E. coli, Aeromonas, and Salmonella in a single test medium. A new class of nonchromogenic substrates is disclosed which produce a substantially black, non-diffusible precipitate. This precipitate is not subject to interference from other chromogenic substrates present in the test medium.; In one embodiment, the substrates are selected such that E. coli colonies present in the test medium show as substantially black, general coliforms colonies show in the test medium as a blue-violet color, Aeromonas colonies present in the test medium show as a generally red-pink color, and Salmonella colonies show as a generally teal-green color. Other microorganisms and color possibilities for detection and quantification thereof are also disclosed. An inhibitor and method for making a test medium incorporating the inhibitor are disclosed.
摘要翻译：一种测试介质和方法，用于在测试样品中检测，定量，识别和区分最多四个（4）单独的生物材料。测试介质是游离缺失盘，其允许定量和下生物实体产生特异的酶，其中可能包括一般形式的大肠杆菌，大肠杆菌，气单胞菌和沙门氏菌在单个测试介质的环境光聚集体区分。一类新的nonchromogenic基板的是游离缺失盘能产生基本上黑色的，非扩散性沉淀物。该沉淀是不受从其他存在于测试介质发色底物的干扰。在一个，实施例的基板被选择的搜索做大肠杆菌存在于测试介质显示为基本上黑色的菌落，一般大肠杆菌形成菌落表明在测试介质中的蓝紫色，存在于试验介质节目作为基因反弹气单胞菌的菌落红粉色，和沙门氏菌菌落显示为一个基因反弹水鸭绿色。用于检测和定量等微生物和颜色的可能性它们因此游离缺失盘。用于制作测试介质结合的抑制剂的抑制剂和方法游离缺失光盘。

3. 发明授权

EP1776465B1 TEST MEDIA AND QUANTITATIVE OR QUALITATIVE METHOD FOR IDENTIFICATION AND DIFFERENTIATION OF BIOLOGICAL MATERIALS IN A TEST SAMPLE 有权
标题翻译：试验介质和定量或定性方法识别和生物材料分化的测试样品
公开(公告)号：EP1776465B1
公开(公告)日：2011-03-02
申请号：EP05765940.1
申请日：2005-06-08
申请人： Micrology Laboratories L.L.C.
发明人： ROTH, Geoffrey, N. , ROTH, Jonathan, N.
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q1/045 , C12Q1/10 , C12Q2334/00 , C12Q2334/50 , G01N2333/245 , G01N2333/25 , G01N2333/255 , G01N2333/924 , Y02A50/451
摘要： A test medium and method for detecting, quantifying, identifying and differentiating up to four (4) separate biological materials in a test sample. A test medium is disclosed which allows quantifying and differentiating under ambient light aggregates of biological entities producing specific enzymes, which might include general coliforms, E. coli, Aeromonas, and Salmonella in a single test medium. A new class of nonchromogenic substrates is disclosed which produce a substantially black, non-diffusible precipitate. This precipitate is not subject to interference from other chromogenic substrates present in the test medium.; In one embodiment, the substrates are selected such that E. coli colonies present in the test medium show as substantially black, general coliforms colonies show in the test medium as a blue-violet color, Aeromonas colonies present in the test medium show as a generally red-pink color, and Salmonella colonies show as a generally teal-green color. Other microorganisms and color possibilities for detection and quantification thereof are also disclosed. An inhibitor and method for making a test medium incorporating the inhibitor are disclosed.

4. 发明授权

EP0897428B1 TEST MEDIA AND QUANTITATIVE METHOD FOR IDENTIFICATION AND DIFFERENTIATION OF BIOLOGICAL MATERIALS IN A TEST SAMPLE 失效
标题翻译：试验介质和定量检测方法的材料和生物材料的歧视测试样品
公开(公告)号：EP0897428B1
公开(公告)日：2007-06-06
申请号：EP97915186.7
申请日：1997-03-21
申请人： RCR Scientific, Inc.
发明人： ROTH, Jonathan, N. , BONTRAGER, Gordon, L.
IPC分类号： C12Q1/04 , C12Q1/54 , C12Q1/18 , C12Q1/02 , C12Q1/22 , C12N5/00 , C12N1/00 , C12Q1/10 , C12Q1/34
CPC分类号： C12Q1/04 , C12Q1/10 , C12Q2334/50 , C12Q2334/52 , G01N2333/24 , Y10S435/849 , Y10S435/879
摘要： A test method and medium for quantitatively identifying and differentiating biological materials in a test sample containing a plurality of different biological materials. A first biological material in the sample has enzyme specificity for a first chromogenic substrate, a second biological material has enzyme specificity for a second chromogenic substrate, and a third biological material has enzyme specificity for one of the chromogenic substrates. The chromogenic substrates form respective first and second colored water insoluble compounds upon reaction with specific enzymes from the biological materials. The first and second biological materials are capable of fermenting a sugar, and the third biological material does not ferment the sugar. The test medium is adjusted to a pH conducive for color change of a pH indicator upon acidification of a portion of the test medium due to fermentation of the sugar, which color change results in the formation of a colored zone around the water insoluble compounds of the sugar-fermenting biological materials, the colored zone being distinguishable from the colors of the water insoluble compounds.

5. 发明授权

EP1297177B1 DETECTION ET/OU DISCRIMINATION DES BACTERIES DU GENRE VIBRIO 有权
标题翻译：检测和/或弧菌属细菌的歧视
公开(公告)号：EP1297177B1
公开(公告)日：2004-11-24
申请号：EP01949540.7
申请日：2001-06-27
申请人： Rambach, Alain
发明人： Rambach, Alain
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q2334/50 , G01N2333/28 , G01N2333/924 , Y02A50/52
摘要： The invention concerns a culture medium for isolating Vibrio bacteria, characterised in that it comprises, in a Vibrio culture medium, at least a chromogenic agent selected among the beta -glucosidase substrates and the beta -galactosidase substrates.

6. 发明公开

EP1334205A1 PROCEDE D'IDENTIFICATION DE LISTERIA MONOCYTOGENES ET MILIEU DE CULTURE 有权
标题翻译：一种用于识别MONOZYTOGENEN增生李斯特菌和培养基
公开(公告)号：EP1334205A1
公开(公告)日：2003-08-13
申请号：EP01996621.7
申请日：2001-11-19
申请人： Biomerieux S.A.
发明人： ROGER-DALBERT, Céline , BARBAUX, Laurence
IPC分类号： C12Q1/04 , C12Q1/44 , C12Q1/37 , C12Q1/42 , C12N1/20
CPC分类号： C12N1/20 , C12N9/16 , C12Q1/04 , C12Q1/045 , C12Q1/34 , C12Q1/37 , C12Q1/42 , C12Q1/44 , C12Q2334/50
摘要： The invention concerns a substrate for directly identifying pathogenic bacteria of the genus Listeria. It consists in detecting an esterase activity, different from a Phosphatidylinositol (PI-PLC)-specific phospholipase C activity, specific of esterase activity which is specific of the Listeria monocytogenes species. The invention also concerns the association of two substrates, one as described above and the other specific of all or part of the genus Listeria. The invention further concerns a reaction medium containing such a substrate or such a combination of substrates. Finally, the invention concerns an identification method using such culture media. The invention is particularly applicable in the field of diagnosis.

7. 发明公开

EP1297177A1 DETECTION ET/OU DISCRIMINATION DES BACTERIES DU GENRE VIBRIO 有权
标题翻译：检测和/或弧菌属细菌的歧视
公开(公告)号：EP1297177A1
公开(公告)日：2003-04-02
申请号：EP01949540.7
申请日：2001-06-27
申请人： Rambach, Alain
发明人： Rambach, Alain
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q2334/50 , G01N2333/28 , G01N2333/924 , Y02A50/52
摘要： The invention concerns a culture medium for isolating Vibrio bacteria, characterised in that it comprises, in a Vibrio culture medium, at least a chromogenic agent selected among the β-glucosidase substrates and the β-galactosidase substrates.

8. 发明公开

EP1045923A1 METHOD FOR ISOLATION AND IDENTIFICATION OF (ESCHERICHIA COLI) 0157:H7 AND PLATING MEDIA FOR SAID PROCESS 失效
标题翻译：大肠杆菌（大肠杆菌）0157：H7的分离鉴定和鉴定方法
公开(公告)号：EP1045923A1
公开(公告)日：2000-10-25
申请号：EP97944436.1
申请日：1997-09-16
申请人： R & F Laboratories, Inc.
发明人： RESTAINO, Lawrence
IPC分类号： C12Q1/04
CPC分类号： C12Q1/10 , C12Q2334/50 , C12Q2334/52 , G01N2333/245
摘要： A solid plating medium for the presumptive detection of Escherichia coli 0157:H7 which selectively promotes growth of Escherichia coli, inhibits growth of gram positive microorganims, inhibits growth of Proteus species, inhibits the growth of Escherichia coli other than Escherichia coli 0157:H7, wherein said plating medium includes agar, a carbohydrate medium which is not fermented by Escherichia coli 0157:H7 but is fermented by other microorganisms to include other strains of Escherichia coli and results in a first color when fermented, a chromogenic beta-galactosidase substrate which produces a second color when fermented by beta-galactosidase, and a pH indicator dye which changes said medium to a third color.
摘要翻译：推定检测选择性促进大肠杆菌生长，抑制革兰氏阳性微生物生长，抑制变形菌种生长，抑制除大肠杆菌0157：H7以外的大肠杆菌生长的大肠杆菌O157：H7的固体培养基，其中所述平板培养基包括琼脂，即不被大肠杆菌O157：H7发酵但被其他微生物发酵以包含其他大肠杆菌菌株并在发酵时产生第一种颜色的碳水化合物培养基，所述发色β-半乳糖苷酶底物产生当由β-半乳糖苷酶发酵时为第二颜色，以及将所述介质改变为第三颜色的pH指示剂染料。

9. 发明公开

EP0897428A1 TEST MEDIA AND QUANTITATIVE METHOD FOR IDENTIFICATION AND DIFFERENTIATION OF BIOLOGICAL MATERIALS IN A TEST SAMPLE 失效
标题翻译：试验介质和定量检测方法的材料和生物材料的歧视测试样品
公开(公告)号：EP0897428A1
公开(公告)日：1999-02-24
申请号：EP97915186.0
申请日：1997-03-21
申请人： RCR Scientific, Inc.
发明人： ROTH, Jonathan, N. , BONTRAGER, Gordon, L.
IPC分类号： C12Q1 , C12N1
CPC分类号： C12Q1/04 , C12Q1/10 , C12Q2334/50 , C12Q2334/52 , G01N2333/24 , Y10S435/849 , Y10S435/879
摘要： A test method and medium for quantitatively identifying and distinguishing biological materials in a test sample. A first biological material has enzyme specificity for a first chromogenic substrate, a second biological material has enzyme specificity for a second chromogenic substrate, and a third biological material has specificity for one of the substrates. The chromogenic substrates form respective first and second colored water insoluble compounds upon reaction with specific enzymes. The first and second biological materials are capable of fermenting a sugar, and the third biological material does not ferment the sugar. The test medium is adjusted to a pH conducive for color change of a pH indicator upon fermentation, resulting in the formation of a zone of a third color around the water insoluble compounds of the fermenting materials. Following incubation, the sample is examined for the presence of colonies of the first biological material, having the first color and an encircling zone of the third color; for colonies of the second material, having the second color and the colored zone; and for colonies of a third material having the first or second color, and not having the colored zone.

10. 发明授权

EP0711360B1 Procédé d'identification de microorganismes à l'aide d'au moins deux chromogènes 有权
标题翻译：通过至少两个发色团的装置，用于微生物的测定方法
公开(公告)号：EP0711360B1
公开(公告)日：1998-03-11
申请号：EP94923761.4
申请日：1994-07-28
申请人： Rambach, Alain
发明人： Rambach, Alain
IPC分类号： C12Q1/04 , C12Q1/10
CPC分类号： C12Q1/10 , C12Q1/04 , C12Q2334/50 , C12Q2334/52
摘要： A method for revealing the presence or absence of a particular microorganism strain in a culture medium, wherein at least two strain enzyme substrate chromogens are added to the culture medium, said chromogens being selected so that the presence of said strain in the culture medium is revealed by a third colour.

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