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1. 发明公开

EP3033428A1 ENZYMATIC HYDROLYSIS OF ACYLATED STERYL GLYCOSIDES AND METHOD FOR TREATING BIOFUEL 审中-公开
标题翻译：酰化的酶水解固醇糖苷和方法用于治疗的生物燃料
公开(公告)号：EP3033428A1
公开(公告)日：2016-06-22
申请号：EP14750507.7
申请日：2014-08-13
申请人： ETH Zurich
发明人： MÜNGER, Linda , NYSTRÖM, Laura
IPC分类号： C12P7/64 , C11B3/00
CPC分类号： C10L1/04 , C10L2200/0469 , C10L2290/26 , C11B3/003 , C12P7/6445 , C12P33/00
摘要： The present invention relates to the use of at least one glycosidase of the class EC 3.2.1.21, EC 3.2.1.4, EC 3.2.1.7, and/or EC 3.2.1.80 for hydrolyzing at least one glycosiding bond in at least one substrate selected from the group of acylated steryl glycosides of the general formula (I): R1—CO—O—[CmHnOx]y—O—R2 wherein R1 is a saturated or non-saturated C1-C30 alkyl, R2 is a sterol moiety, m=5-7, n=9-14 and x=5-7, y=1-5. The present invention relates further to a method of for hydrolysing steryl gylcosides using said enzymes in biofuel, oil and/or fat.

2. 发明公开

EP2914725A4 NOVEL ACYLTRANSFERASE POLYNUCLEOTIDES, POLYPEPTIDES, AND METHODS OF USE 审中-公开
标题翻译：新型ACYLTRANSFERASEPOLYNUKLEOTIDE，多肽和使用方法
公开(公告)号：EP2914725A4
公开(公告)日：2016-04-20
申请号：EP13851129
申请日：2013-10-22
申请人： AGRES LTD
发明人： ROBERTS NICHOLAS JOHN , CURRAN AMY CHRISTINA , WINICHAYAKUL SOMRUTAI , ROLDAN MARISSA , SCOTT RICHARD WILLIAM
IPC分类号： C12N15/29 , C12N9/10 , C12N15/82 , C12P7/64
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6445 , C12Y203/0102
摘要： The invention provides a novel DGAT1 protein with improved properties over known DGAT proteins, particularly known DGAT1 proteins from plants. The novel DGAT1 protein of the invention can be expressed in cells to increase cellular lipid accumulation. Expression of the DGAT1 protein of the invention in cells results in a higher level of lipid than any of several other plant DGAT1 proteins tested by the applicants. The invention provides polynucleotides encoding the novel DGAT1 protein of SEQ ID NO:39, constructs, cells, plant, plant parts and progeny comprising the polynucleotides, and methods of use of the polynucleotides and polypeptides of the invention.

3. 发明授权

EP1776455B1 LIPOLYTIC ENZYME, USES THEREOF IN THE FOOD INDUSTRY 有权
标题翻译：脂解酶，它的使用在食品工业
公开(公告)号：EP1776455B1
公开(公告)日：2015-03-18
申请号：EP05773820.5
申请日：2005-07-18
申请人： DuPont Nutrition Biosciences ApS
发明人： MIASNIKOV, Andrei , SØE, Jørn, Borch , MIKKELSEN, Jørn, Dalgaard , POVELAINEN, Mira , PITKANEN, Virve
IPC分类号： C12N9/20 , C12N15/55 , C12P7/64 , A23C9/13 , A21D2/26 , C11B3/00 , C11C3/00 , C12N9/10 , A21D8/04 , A23J7/00
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003

4. 发明公开

EP2702150A1 LIPASE VARIANTS HAVING INCREASED ENZYME SPECIFICITY OR ENHANCED TRANS-SELECTIVITY, AND METHODS OF USE 失效
标题翻译：脂肪酶增大的酶特异性或选择性提高TRANS和使用方法
公开(公告)号：EP2702150A1
公开(公告)日：2014-03-05
申请号：EP12718322.6
申请日：2012-04-27
申请人： Frito-Lay Trading Company GmbH
发明人： BORNSCHEUER, Uwe , BRUNDIEK, Henrike , EVITT, Andrew , SAß, Stefan , BÖNISCH, Friedericke , KOURIST, Robert
IPC分类号： C12N9/20 , C12N1/15 , C12N1/21 , C12P7/64
CPC分类号： C12N9/20 , C11B3/003 , C12P7/6445
摘要： Disclosed are modified lipase enzymes, as well as polynucleotides and recombinant expression vectors encoding the lipase variant polypeptides, as well as methods for producing such lipase variants in selected bacterial and fungal host cells. The invention further relates to lipase variants having a preference for medium- or long- chain fatty acid esters or carboxylic acid moieties with carbon-chain lengths greater than or equal to C
6 . The invention further relates to methods for selectively reducing, or eliminating, one or more trans-fatty acids from substrates, including liquid edible oils useful in food manufacture and frying systems.

5. 发明授权

EP2057274B1 PHOSPHOLIPASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 有权
标题翻译：磷脂酶，NUKLEINSAÜRE，对他们的生产和使用此代码和方法
公开(公告)号：EP2057274B1
公开(公告)日：2013-12-11
申请号：EP07853585.3
申请日：2007-09-21
申请人： DSM IP Assets B.V.
发明人： O'DONOGHUE, Eileen , BARTON, Nelson R.
IPC分类号： C12N9/16 , C12N15/55 , C12P7/64 , C12N15/63 , C12N5/10 , C12N1/19 , C12N1/21 , A61K38/46 , C12P21/02 , A01K67/027 , A01H5/00
CPC分类号： C12P7/649 , C12N9/16 , C12N15/8243 , C12P7/6418 , C12P7/6445 , C12P7/6481 , Y02E50/13

6. 发明授权

EP1805302B1 HYDROLYSIS OF ESTER BONDS WITH LIPASE 有权
标题翻译：脂肪酶的埃斯特·邦兹的水解
公开(公告)号：EP1805302B1
公开(公告)日：2012-08-15
申请号：EP05789094.9
申请日：2005-10-05
申请人： Novozymes A/S
发明人： SCHÄFER, Thomas , ANTRANIKIAN, Garabed , ROYTER, Maryna , HOFF, Tine
IPC分类号： C12N9/20 , C12N15/55 , C12P7/40 , C12P7/62 , C12P7/64 , C12P41/00
CPC分类号： C12P7/6418 , C12N9/20 , C12P7/40 , C12P7/62 , C12P7/6445 , C12P41/004
摘要： The inventors have identified polypeptides having lipase activity in anaerobic thermo-philic bacteria. Accordingly, the invention provides a process for hydrolyzing an ester bond in a substrate, which comprises treating the substrate with a particular lipase (a polypeptide having lipase activity). The invention also provides a lipase for use in the process and a polynucleo-tide encoding the lipase.

7. 发明公开

EP2351845A1 Renewable chemicals and fuels from oleaginous yeast 审中-公开
标题翻译： Erneuerbare Chemikalien und Brennstoffe ausölhaltigerHefe
公开(公告)号：EP2351845A1
公开(公告)日：2011-08-03
申请号：EP11158642.6
申请日：2008-06-02
申请人： Solazyme, Inc.
发明人： Trimbur, Donald , Im, Chung-Soon , Dillon, Harrison , Day, Anthony , Franklin, Scott , Coragliotti, Anna
IPC分类号： C12P5/02 , C12P7/64 , C12N1/32 , C12N9/26 , C10L1/02
CPC分类号： C10L1/026 , C07H21/04 , C10L1/06 , C10L2200/0484 , C10L2270/026 , C10L2270/04 , C11C3/00 , C12N1/12 , C12N1/32 , C12N9/0006 , C12N9/0008 , C12N9/1205 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/2408 , C12N9/2431 , C12N9/88 , C12N15/79 , C12P5/02 , C12P7/6409 , C12P7/6436 , C12P7/6445 , C12P7/6463 , C12P7/649 , C12P21/06 , C12Y302/01026 , Y02E50/10 , Y02E50/13 , Y02E50/343 , Y02P20/52 , Y02T50/678
摘要： The invention provides a method of producing a fuel or a chemical, the method comprising the steps of heterotrophically culturing an oleaginous yeast in the presence of a fixed carbon source, wherein the fixed carbon source is selected from the group consisting of glycerol, depolymerized cellulosic material, sucrose, molasses, glucose, arabinose, galactose, xylose, fructose, mannose, and acetate; and wherein the oleaginous yeast accumulates at least 10% of its dry cell weight as lipid, followed by isolation of the lipid from the oleaginous yeast. The isolated lipid is then subjected to a chemical reaction selected from the group consisting of cracking, hydrotreating, hydroprocessing, indirect liquefaction, hydrodeoxygenation, transesterification, and isomerization, and optionally, distillation.
摘要翻译：本发明提供一种生产燃料或化学品的方法，所述方法包括以下步骤：在固定碳源的存在下异质培养含油酵母，其中固定碳源选自甘油，解聚纤维素材料蔗糖，糖蜜，葡萄糖，阿拉伯糖，半乳糖，木糖，果糖，甘露糖和乙酸酯; 并且其中所述含油酵母将其干细胞重量的至少10％作为脂质积聚，随后从含油酵母中分离出脂质。然后将分离的脂质进行选自裂化，加氢处理，加氢处理，间接液化，加氢脱氧，酯交换和异构化，以及任选地蒸馏的化学反应。

8. 发明授权

EP1791933B1 ENZYMATIC OIL-DEGUMMING METHOD 有权
标题翻译： ENZYMATIC OIL-DEGUMMING法
公开(公告)号：EP1791933B1
公开(公告)日：2011-06-29
申请号：EP05761577.5
申请日：2005-07-18
申请人： DANISCO A/S
发明人： SOE, Jorn, Borch , TURNER, Mark
IPC分类号： C11B3/00 , C11C3/00 , C07F9/10
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： A process of enzymatic degumming edible oils, comprising treating the edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxy group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprises one or more of the following amino acid sequences: SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, or SEQ ID No. 15, SEQ ID No. 16, SEQ ID No. 17, SEQ ID No. 18, SEQ ID No. 36, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No 45, SEQ ID No. 47, SEQ ID No. 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID No. 16 is also taught.
摘要翻译：一种酶促脱胶食用油的方法，包括用脂质酰基转移酶处理食用油以便将酰基从大部分磷脂转移到一个或多个酰基受体，其中酰基受体可以是任何含有羟基的化合物。在一个实施方案中，优选酰基受体是水，在另一个实施方案中，优选酰基受体是一种或多种甾醇和/或甾烷醇。当酰基受体是甾烷醇和/或甾醇时，产生一种或多种甾醇酯和/或甾烷醇酯。用于本发明方法的脂质酰基转移酶可以包含一个或多个以下氨基酸序列：SEQ ID No.1，SEQ ID No.3，SEQ ID No.4，SEQ ID No.5，SEQ ID No.5 序列号6，序列号7，序列号8，序列号9，序列号10，序列号11，序列号12，序列号13，序列号 14或SEQ ID No.15，SEQ ID No.16，SEQ ID No.17，SEQ ID No.18，SEQ ID No.36，SEQ ID No.38，SEQ ID No.40，SEQ ID No.41 ，SEQ ID No 45，SEQ ID No.47，SEQ ID No.50或与其具有75％或更多同一性的氨基酸序列。还教导了包含如SEQ ID No.16所示的氨基酸序列的新型脂质酰基转移酶。

9. 发明公开

EP2329032A2 HYDROLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 有权
标题翻译：使用具有水解酶活性的多肽的方法
公开(公告)号：EP2329032A2
公开(公告)日：2011-06-08
申请号：EP09789233.5
申请日：2009-08-28
申请人： Bunge Oils, Inc
发明人： DAYTON, Christopher, L., G. , BARTON, Nelson , BUENO, Analia , CUENCA, Joslin, G. , HITCHMAN, Tim , KLINE, Katie, A. , LYON, Jonathan , MILLER, Mark, L. , WALL, Mark, A.
IPC分类号： C12P7/64
CPC分类号： C12P7/6472 , A23D7/04 , A23D9/04 , C11B3/003 , C11C1/045 , C11C3/08 , C11C3/12 , C12N9/20 , C12P7/6418 , C12P7/6427 , C12P7/6445 , C12P7/6454
摘要： Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.

10. 发明公开

EP2267108A1 Enzymatic oil-degumming method 无效转让
标题翻译：酶促油脱胶法
公开(公告)号：EP2267108A1
公开(公告)日：2010-12-29
申请号：EP10174207.0
申请日：2005-07-18
申请人： Danisco A/S
发明人： Soe, Jørn Borch , Turner, Mark
IPC分类号： C11B3/00 , C11C3/00 , C07F9/10
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： A process of enzymatic degumming edible oils, comprising treating the edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxy group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprises one or more of the following amino acid sequences: SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, or SEQ ID No. 15, SEQ ID No. 16, SEQ ID No. 17, SEQ ID No. 18, SEQ ID No. 36, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No 45, SEQ ID No. 47, SEQ ID No. 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID No. 16 is also taught.
摘要翻译：一种酶促脱胶食用油的方法，包括用脂质酰基转移酶处理食用油以便将酰基从大部分磷脂转移到一个或多个酰基受体，其中酰基受体可以是任何含有羟基的化合物。在一个实施方案中，优选酰基受体是水，在另一个实施方案中，优选酰基受体是一种或多种甾醇和/或甾烷醇。当酰基受体是甾烷醇和/或甾醇时，产生一种或多种甾醇酯和/或甾烷醇酯。用于本发明方法的脂质酰基转移酶可以包含一个或多个以下氨基酸序列：SEQ ID No.1，SEQ ID No.3，SEQ ID No.4，SEQ ID No.5，SEQ ID No.5 序列号6，序列号7，序列号8，序列号9，序列号10，序列号11，序列号12，序列号13，序列号 14或SEQ ID No.15，SEQ ID No.16，SEQ ID No.17，SEQ ID No.18，SEQ ID No.36，SEQ ID No.38，SEQ ID No.40，SEQ ID No.41 ，SEQ ID No 45，SEQ ID No.47，SEQ ID No.50或与其具有75％或更多同一性的氨基酸序列。还教导了包含如SEQ ID No.16所示的氨基酸序列的新型脂质酰基转移酶。

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