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1. 发明公开

EP2446027A1 PROTEIN 审中-公开
标题翻译：蛋白质
公开(公告)号：EP2446027A1
公开(公告)日：2012-05-02
申请号：EP10730253.1
申请日：2010-06-23
申请人： Danisco A/S
发明人： MIASNIKOV, Andrei , BOTT, Richard, R , SØRENSEN, Jens, Frisbæk
IPC分类号： C12N9/18 , C12N9/20 , A23L1/015 , C12N15/55
CPC分类号： C12N9/20 , A21D8/042 , C12N9/18 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032
摘要： The present invention relates to a method for preparing a variant lipolytic enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a fungal lipolytic enzyme and comprises at at least one modification at a position which corresponds in the encoded amino acid sequence to a) the introduction of at least one glycosylation site (or one additional glycosylation site) in the amino acid sequence compared with the original fungal lipolytic enzyme; b) the introduction of at least one amino acid at a surface position on the polypeptide and at a location in an external loop distal to the active site (catalytic triad) of the enzyme which is more hydrophilic (compared with the original amino acid); or c) a substitution or insertion at one or more of positions 33, 63, 78, 190, 305, 306 or 320 or a deletion at one or more positions 311-312 or 307-319, wherein each amino acid position corresponds to the position of the amino acid sequence when aligned with SEQ ID No. 2; wherein when the nucleotide sequence has at least 90% identity with a nucleotide sequence encoding the fungal lipolytic enzyme shown in SEQ ID No. 22 or SEQ ID No. 23 the modification is not a substitution at position 63 and the deletion is not at position 311 -312. Preferably the nucleotide sequence has at least 90% identity with SEQ ID No. 1. The invention also relates to polypeptide produced by the method and to novel nucleic acids.

2. 发明授权

EP1711064B1 BAKED PRODUCT TREATED WITH NATAMYCIN AND PROCESS THEREOF 有权
标题翻译：纳他霉素经处理焙烤制品及其方法
公开(公告)号：EP1711064B1
公开(公告)日：2012-04-11
申请号：EP05707851.1
申请日：2005-01-26
申请人： DANISCO A/S
发明人： WILLIAMS, Graham , DELVES-BROUGHTON, Joss , FARAGHER, John , SALMELA, Diane , HARDY, Jay , HAUGAN, Kersti , THOMAS, Linda , WISLER, Peter
IPC分类号： A21D15/08 , A23L3/3463
CPC分类号： A23L3/34635 , A21D15/08
摘要： The present invention relates to a non yeast-leavened fine bakery product with increased shelf life and to a process for increasing the shelf life of intermediate and high moisture fine bakery products. The fine bakery product comprises an intermediate or high moisture baked product having a water activity aw >0.8. Its surface has deposited thereon an effective amount of natamycin, which is sufficient to keep the product mould free when packaged for a storage time of 2 weeks or more at ambient temperature. In the process the outer surface of a baked product is sprayed with natamycin to deposit an effective amount of natamycin thereon, whereafter the sprayed product is packaged in a protective envelope.

3. 发明公开

EP2432876A1 USE 审中-公开
标题翻译：抑制剂Zdhhc2的活性的调制使用脂肪形成
公开(公告)号：EP2432876A1
公开(公告)日：2012-03-28
申请号：EP10727890.5
申请日：2010-05-19
申请人： Danisco A/S
发明人： SØRENSEN, Bo Spange , POVLSEN, Inge Lise , MEJLDAL, Rie , KRAGH, Karsten Matthias , KELLETT-SMITH, Anja Hemmingsen , MIKKELSEN, Rene , JENNER, Rikke L. Bundgaard
IPC分类号： C12N9/28
CPC分类号： C12N9/20 , A21D8/042 , C12N9/1029 , C12N9/2417
摘要： The present invention relates to the use of an amylase and a lipolytic enzyme in combination to improve the stackability of bread, methods of preparing dough and baked products having a combination of such enzymes, as well as bread having particular bread stackability profiles.

4. 发明公开

EP2427526A1 USE OF SOLID BETAINE PRODUCT AND METHOD FOR ITS MANUFACTURE 有权转让
标题翻译：使用ENES扎实甜菜碱产品VEFAHREN其生产
公开(公告)号：EP2427526A1
公开(公告)日：2012-03-14
申请号：EP10772067.4
申请日：2010-05-06
申请人： Danisco A/S
发明人： NURMI, Juha , JUTILA, Kirsti , PAANANEN, Hannu , ERIKSSON, Kristian
IPC分类号： C09K3/18 , C07C229/12 , C07C227/40 , E01H10/00 , B01J2/30
摘要： The invention relates to the use of solid betaine for de-icing and/or preventing slipperiness. The solid betaine comprises at least one non-betaine compound affecting water and/or moisture movement in said solid betaine. The invention further relates to a process for the manufacture of solid betaine. A suspension comprising betaine crystals is prepared from a feed liquid and the betaine crystals are separated and washed. The amount of wash liquid is adjusted in order to leave 0.5 to 10 w-% of at least one non-betaine compound in said solid betaine.

5. 发明授权

EP1366198B1 SEPARATION PROCESS 有权
标题翻译：分离
公开(公告)号：EP1366198B1
公开(公告)日：2012-02-08
申请号：EP01994869.4
申请日：2001-12-28
申请人： Danisco A/S
发明人： HEIKKILÄ, Heikki , MÄNTTÄRI, Mika , NYSTRÖM, Marianne , LINDROOS, Mirja , PAANANEN, Hannu , PUUPPO, Outi , KOIVIKKO, Hannu
IPC分类号： C13K1/00 , C13K13/00 , C13B20/16
CPC分类号： C13B20/165 , C13K13/002
摘要： The invention relates to a process of separating compounds with a small molar mass from compounds having a molar mass less than 1.9 times that of the compounds with a small molar mass using nanofiltration. The compounds with a small molar mass have a typical molar mass less than 250 g/mol. In one embodiment of the invention, pentose sugars are separated from hexose sugars. The invention can be applied to the recovery of xylose from spent liquors and to the recovery of betaine from sugar beet pulp extracts, for example.

6. 发明公开

EP2375916A1 PROCÉDÉ DE FABRICATION DE LAIT MATURÉ POUR L'ALIMENTATION D'ANIMAUX NOURRIS AU LAIT 有权转让
标题翻译：用于生产BEREIFERTE奶食品类乳GEFUTTERTE动物
公开(公告)号：EP2375916A1
公开(公告)日：2011-10-19
申请号：EP10706011.3
申请日：2010-01-07
申请人： Danisco A/S
发明人： BERGER, Claudette
IPC分类号： A23K1/18 , A23C9/123
CPC分类号： A23K50/60 , A23K10/16 , A23K10/28 , A23K50/10 , A23V2002/00 , Y02P60/875 , A23V2200/32 , A23V2200/3204 , A23V2200/06
摘要： The invention relates to a method for producing cured milk for feeding milk-fed animals, particularly livestock.

7. 发明公开

EP2275522A3 Enzymatic oil-degumming method 审中-公开
标题翻译：酶促油脱胶法
公开(公告)号：EP2275522A3
公开(公告)日：2011-09-21
申请号：EP10175653.4
申请日：2005-07-18
申请人： Danisco A/S
发明人： Miasnikov, Andrei , Soe, Jørn , Mikkelsen, Jørn , Povelainen, Mira , Pitkanen, Virve
IPC分类号： C11B3/00 , C11C3/00 , C07F9/10 , A23J7/00 , C12N9/20 , C12P7/64 , A21D8/04
CPC分类号： C12N9/1029 , A21D8/042 , A23J7/00 , C11B3/003 , C12N9/20 , C12P7/6418 , C12P7/6445 , C12P7/6481 , C12Y301/01003
摘要： The invention also encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to a acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ. ID. No.s 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID No. 3, 6, 9, 13, 15 or 17 or a nulceotide seqeunce which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Steptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID No. 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of:
a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3;
a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3 by the degeneration of the genetic code; and
a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID No. 3.
摘要翻译：本发明还包括在各种方法和用途中可使用从下列属之一获得的脂解酶：链霉菌属，棒状杆菌属和嗜热菌属，其中所述脂解酶能够水解糖脂或磷脂或从糖脂转移酰基或磷脂酰基受体。优选地，用于这些方法和用途的脂解酶包含如SEQ.ID.NO.1中任一所示的氨基酸序列。 ID。编号为4,5,7,8,12,14或16的氨基酸序列或与其具有至少70％同一性的氨基酸序列，或者包含如SEQ ID No.3,6,9,13,15或17所示的核苷酸序列或与其具有至少70％同一性的核苷酸序列。本发明还涉及能够水解至少半乳糖脂或能够将酰基从半乳糖脂转移到一个或多个酰基受体底物的脂肪分解酶，其中所述酶可以从链霉菌种获得并且包括含有氨基酸的脂解酶如SEQ ID No.4所示的氨基酸序列或与其具有至少60％同一性的氨基酸序列。该酶可由选自以下的核酸编码：包含SEQ ID No.3所示核苷酸序列的核酸; 通过遗传密码的变性与核苷酸序列SEQ ID No.3相关的核酸; 和包含与SEQ ID No.3中所示的核苷酸序列具有至少70％同一性的核苷酸序列的核酸。

8. 发明授权

EP1252127B1 A PROCESS FOR THE CRYSTALLIZATION OF BETAINE MONOHYDRATE AND ANHYDROUS BETAINE 有权
标题翻译：结晶betainemonohydrate和无水甜菜碱的方法
公开(公告)号：EP1252127B1
公开(公告)日：2011-05-18
申请号：EP01948977.2
申请日：2001-01-16
申请人： DANISCO A/S
发明人： ERIKSSON, Kristian , NURMI, Juha , VIRTANEN, Jouko
IPC分类号： C07B63/00 , C07D201/16 , C13B30/02
CPC分类号： C13B30/027 , C07B63/00 , C07D201/16 , C30B7/00
摘要： The invention relates to a process for the crystallization of substances having a narrow metastable supersaturation zone. In the process the saturation of a solution is gradually increased and the solution is seeded for the crystallization. The seeding is performed at a seeding point which is selected in response to a signal received from said process indicating imminent or initial spontaneous nucleation. The process provides a good crystal yield and a crystalline product having a uniform crystal structure and a narrow crystal size distribution.

9. 发明公开

EP2318022A1 NEW USES OF LACTIC ACID BACTERIA AND BIFIDOBACTERIA 审中-公开
标题翻译：牛奶和新用途乳酸菌双歧杆菌
公开(公告)号：EP2318022A1
公开(公告)日：2011-05-11
申请号：EP09766203.5
申请日：2009-06-19
申请人： Danisco A/S
发明人： BURCELIN, Rémy , CARCANO, Didier , DESREUMAUX, Pierre , LAHTINEN, Sampo
IPC分类号： A61K35/74 , A61P3/10 , A61P9/00
CPC分类号： A61K35/74 , A61K35/744 , A61K35/745 , A61K35/747
摘要： The invention comprises use of a bacterium selected from a lactic acid bacterium, a Bifidobacterium and a mixture of any thereof in the manufacture of a food product, dietary supplement or medicament for lowering tissue inflammation (in particular, adipose, liver or muscle tissue inflammation) and treating cardiovascular disease in a mammal. The lactic acid bacteria and/or Bifidobacteria can also be used to treat diabetes and insulin resistance and induce enhanced secretion of insulin upon a glucose challenge and lower blood glucose levels without a concomitant decrease in weight gain.

10. 发明公开

EP2315840A2 A METHOD OF GLYCOSYLATING A LIPID 审中-公开
标题翻译：脂质糖基化方法
公开(公告)号：EP2315840A2
公开(公告)日：2011-05-04
申请号：EP09786133.0
申请日：2009-07-16
申请人： Danisco A/S
发明人： KRAGH, Karsten, Mathias , MEJLDAL, Rie , MIKKELSEN, Rene
IPC分类号： C12P19/44 , A21D10/00
CPC分类号： C12P19/44 , A21D8/042
摘要： A method of glycosylating a lipid having a free hydroxyI group, the method comprising contacting said lipid with a source of monosaccharide moiety and a transglycosidase enzyme, is disclosed. In particular, a method for in situ production of the glycosylated lipid in a food product is disclosed.

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