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1. 发明授权

EP1585384B1 Precise breeding 有权
标题翻译：精密养殖
公开(公告)号：EP1585384B1
公开(公告)日：2012-07-11
申请号：EP03716081.9
申请日：2003-02-20
申请人： J.R. Simplot Company
发明人： ROMMENS, Caius, M.T. , YE, Jingsong , YAN, Hua , SWORDS, Kathleen, M.M. , MENENDEZ-HUMARA, Jaime , LEIGH BRINKERHOFF, W , RICHAEL, Craig
IPC分类号： C12N15/29 , C12N15/82 , C12N15/84 , A01H5/00
CPC分类号： C12N15/8202 , A01H1/00 , A01H1/04 , A23L5/11 , A23L19/12 , A23L19/18 , C07K14/415 , C12N9/0059 , C12N9/1051 , C12N9/1294 , C12N15/8205 , C12N15/8209 , C12N15/821 , C12N15/8216 , C12N15/8218 , C12N15/8226 , C12N15/8243 , C12N15/8245 , C12N15/825 , C12N15/8273
摘要： The present invention relates to a new plant breeding process. The process improves the agronomic performance of crop plants by using genetic material that is also used in classical breeding. Instead of sexually recombining entire genomes at random, as is done in classical breeding, specific genetic elements are rearranged in vitro and inserted back into individual plant cells. Plants obtained through this new plant breeding process do not contain foreign nucleic acid but only contain nucleic acid from the plant species selected for transformation or plants that are sexually compatible with the selected plant species. Plants developed through this new plant breeding process are provided. In particular, potato plants displaying improved tuber storage and health characteristics are provided.

2. 发明公开

EP2121932A1 GENETICALLY MODIFIED PLANTS WHICH SYNTHESIZE A LOW AMYLOSE STARCH WITH INCREASED SWELLING POWER 审中-公开
标题翻译：具有低直链淀粉增加的动力源合成，转基因植物实力
公开(公告)号：EP2121932A1
公开(公告)日：2009-11-25
申请号：EP08707320.1
申请日：2008-01-23
申请人： Bayer CropScience AG
发明人： FROHBERG, Claus , SCHMIDT, Ralf-Christian
IPC分类号： C12N15/82 , C12N9/10 , C12N9/12 , A01H5/00 , A01H5/10 , C08B30/00 , A23L1/00
CPC分类号： C12N15/8245 , A23L29/212 , C12N9/1051 , C12N9/1294
摘要： Genetically modified monocot plant cells producing starch with an amylose content below 5 wt.% have increased starch synthase II and glucan-water dikinase activities compared with the wild type. Independent claims are also included for: (1) monocot plant comprising genetically modified cells as above; (2) propagation material of plants as above; (3) producing a genetically modified monocot plant by modifying a plant cell by introducing a genetic modifications that increase starch synthase II activity, increase glucan-water dikinase activity and reduce granule-bound starch synthase I activity; regenerating plants from the cell; and optionally using these plants to produce more plants; (4) producing modified starch by extracting starch from plant cells, plants or propagation material as above; (5) modified starch produced as above; (6) producing derivatized starch by derivatizing modified starch as above; (7) derivatized starch produced as above; (8) flour comprising modified starch as above; (9) products comprising starch or flour as above.
摘要翻译：遗传修饰的单子叶植物细胞中生产具有高直链淀粉含量的淀粉至低于5％的增加的淀粉合成酶II和葡聚糖水二激酶活性与野生型相比重量.. 独立权利要求，因此包括用于：（1）单子叶植物，其包含基因修饰的细胞如上; （2）如上述植物的繁殖材料; （3）通过引入遗传修饰改变植物细胞中生产遗传修饰的单子叶植物也增加淀粉合成酶II活性，增加葡聚糖水二激酶活性和减少颗粒结合淀粉合成酶I活性; 从所述细胞再生植物; 和任选地使用这些植物产生更多的植物; （4）通过提取从植物细胞，植物或如上述繁殖材料产淀粉的改性淀粉; （5）如上述制得的改性淀粉; （6）生产淀粉通过衍生的改性淀粉如上述衍生化; （7）如上述制得衍生化的淀粉; （8）包含面粉改性淀粉如上述; （9）产品，其包括淀粉或面粉如上。

3. 发明公开

EP0690128A1 POLYPEPTIDE HAVING COLD-RESISTANT PYRUVATE PHOSPHATE DIKINASE ACTIVITY, DNA CODING FOR THE SAME, AND RECOMBINANT VECTOR AND TRANSFORMED PLANT BOTH CONTAINING SAID DNA 失效
标题翻译：具有耐寒PYRUVATPHOSPHATDIKINASE活动中，DNA编码该重组VAKTOR多肽转化植物这两包括DNA
公开(公告)号：EP0690128A1
公开(公告)日：1996-01-03
申请号：EP95902286.4
申请日：1994-12-01
申请人： Japan Tobacco Inc.
发明人： OHTA, Shozo , USAMI, Satoru , BURNELL, James Nigel, Dep. of Molecular Sciences,
IPC分类号： C12N15/54 , C12N9/12 , C12N5/00
CPC分类号： C12N9/1294 , C12N15/8273
摘要： A novel polypeptide having a cold-resistant pyruvate phosphate dikinase activity; a cloned DNA coding for the polypeptide; and a recombinant vector containing the DNA, each as a means for imparting cold resistance to plants. The polypeptide is either the following polypeptide (1) or polypeptide (2) and has such an amino acid sequence that at least one amino acid residue present in the region having a length of one-sixth of the whole length starting with the C-terminus has been replaced by another amino acid residue and also has a cold-resistant pyruvate phosphate dikinase activity: 1) a polypeptide having an amino acid sequence represented by any of the SEQ ID no:1 to no:4 in the sequence listing and also having a pyruvate phosphate dikinase activity; and 2) a polypeptide having such an amino acid sequence that at least 50 % thereof is homologous with the amino acid sequence mentioned in item 1) and also having a pyruvate phosphate dikinase activity.
摘要翻译：具有耐寒丙酮酸磷酸二激酶活性的新的多肽; 克隆的DNA编码的多肽; 和含有该DNA，各作为一种手段用于赋予植物抗寒性的重组载体。所述多肽是下列任一多肽（1）或多肽（2）和在氨基酸序列已经审查做至少一个氨基酸残基存在于具有开始与C末端的整个长度的六分之一的长度的区域已取代另一个氨基酸残基，并因此具有耐寒丙酮酸磷酸二激酶活性：1）具有由任何SEQ ID没有表示的氨基酸序列的多肽：1至NO：清单4的序列中，因此具有丙酮酸磷酸二激酶活性; 和2）在氨基酸序列经审查的多肽做其至少50％同源，在第1项），并因此具有丙酮酸磷酸二激酶活性所提到的氨基酸序列。

5. 发明公开

EP2473597A4 PRODUCTION OF SECRETED BIOPRODUCTS FROM PHOTOSYNTHETIC MICROBES 审中-公开
标题翻译：生产分泌的有机产品光合作用，芽
公开(公告)号：EP2473597A4
公开(公告)日：2013-05-15
申请号：EP10814563
申请日：2010-09-03
申请人： HARVARD COLLEGE
发明人： WAY JEFFREY CHARLES , NIEDERHOLTMEYER HENRIKE , WOLFSTAEDTER BERND , SAVAGE DAVID
IPC分类号： C12N1/21 , C12N15/31 , C12N15/52 , C12P19/02
CPC分类号： C07K14/47 , C12N9/0006 , C12N9/0008 , C12N9/1205 , C12N9/1294 , C12N9/88 , C12P7/56 , C12P13/14 , C12P19/02

6. 发明公开

EP2222855A4 PLANTS WITH MODIFIED STARCH METABOLISM 审中-公开
标题翻译： PFLANZEN MIT MODIFIZIERTEMSTÄRKESTOFFWECHSEL
公开(公告)号：EP2222855A4
公开(公告)日：2011-08-24
申请号：EP08853342
申请日：2008-11-27
申请人： COMMW SCIENT IND RES ORG , GRAINS RES & DEV CORP
发明人： RAL JEAN-PHILIPPE FRANCOIS MICHEL , LI ZHONGYI , MORELL MATTHEW KENNEDY
IPC分类号： C12N15/82 , A01H5/00 , A23L7/10 , A23L7/104 , A23L7/109 , C12N9/00 , C12N9/12 , C12N15/29
CPC分类号： C12N15/8245 , C07K14/415 , C12N9/1294 , C12Y207/09004
摘要： The specification provides methods of obtaining a genetically modified plant which has increased production potential compared to a control plant, the method comprising the steps of i) obtaining a plurality of plants at least one of which comprises in its genome a heterologous polynucleotide, ii) identifying from the plurality of plants a plant which has increased production potential relative to the control plant and comprises the heterologous polynucleotide, and iii) selecting the genetically modified plant, wherein the polynucleotide comprises a transcriptional control sequence operably linked to a nucleic acid sequence which encodes an agent that modifies endogenous starch phosphorylation and/or starch degradation in the plant. In some embodiments, the plant has increased endogenous glycosylase or increased digestibility compared to a control plant. In some specific embodiments, the endogenous starch phosphorylation and/or starch degradation is modified by modifying expression or activity of one or more enzymes selected from the group consisting of α-amylase (EC 3.2.1.1), &bgr;-amylase (EC 3.2.1.2), glucoamylase (EC 3.2.1.3), starch phosphorylase (EC2.4.1.1), glycosylase (EC 3.1.33), sucrase-isomaltase (EC 3.2.10), amylomaltase (EC 2.4.1.25), maltase (EC 3.2.1.20), isoamylase, and α-glucan, water dikinase (GWD, EC 2.7.9.4).
摘要翻译：本说明书提供获得与对照植物相比具有增加的生产潜力的遗传修饰植物的方法，所述方法包括以下步骤：i）获得多个植物，其中至少一个在其基因组中包含异源多核苷酸，ii）鉴定来自多个植物的植物相对于对照植物具有增加的生产潜力并且包含异源多核苷酸，和iii）选择遗传修饰的植物，其中所述多核苷酸包含可操作地连接到编码修饰植物内源性淀粉磷酸化和/或淀粉降解的试剂。在一些实施方案中，与对照植物相比，植物具有增加的内源糖基化酶或增加的消化率。在一些具体实施方案中，内源性淀粉磷酸化和/或淀粉降解通过修饰一种或多种选自α-淀粉酶（EC 3.2.1.1），β-淀粉酶（EC 3.2。 1.2），葡糖淀粉酶（EC 3.2.1.3），淀粉磷酸化酶（EC2.4.1.1），糖基化酶（EC 3.1.33），蔗糖酶异麦芽糖酶（EC 3.2.10），淀粉麦芽糖酶（EC 2.4.1.25），麦芽糖酶 3.2.1.20），异淀粉酶和α-葡聚糖，水二激酶（GWD，EC 2.7.9.4）。

7. 发明公开

EP2248902A3 Precise breeding 有权
标题翻译：精密养殖
公开(公告)号：EP2248902A3
公开(公告)日：2011-03-02
申请号：EP10008266.8
申请日：2003-02-20
申请人： J.R. Simplot Company
发明人： Rommens, Caius M. T. , Ye, Jingsong , Yan, Hua , Swords, Kathleen M. , Menendez-Humara, Jaime , Brinkerhoff, Leigh W. , Richael, Craig
IPC分类号： C12N15/82 , C12N9/10 , A23L1/217 , C12N9/12
CPC分类号： C12N15/8202 , A01H1/00 , A01H1/04 , A23L5/11 , A23L19/12 , A23L19/18 , C07K14/415 , C12N9/0059 , C12N9/1051 , C12N9/1294 , C12N15/8205 , C12N15/8209 , C12N15/821 , C12N15/8216 , C12N15/8218 , C12N15/8226 , C12N15/8243 , C12N15/8245 , C12N15/825 , C12N15/8273
摘要： The present invention relates to a new plant breeding process. The process improves the agronomic performance of crop plants by using genetic material that is also used in classical breeding. Instead of sexually recombining entire genomes at random, as is done in classical breeding, specific genetic elements are rearranged in vitro and inserted back into individual plant cells. Plants obtained through this new plant breeding process do not contain foreign nucleic acid but only contain nucleic acid from the plant species selected for transformation or plants that are sexually compatible with the selected plant species. Plants developed through this new plant breeding process are provided. In particular, potato plants displaying improved tuber storage and health characteristics are provided.

8. 发明公开

EP2248902A2 Precise breeding 有权
标题翻译： Präzisionszüchtung
公开(公告)号：EP2248902A2
公开(公告)日：2010-11-10
申请号：EP10008266.8
申请日：2003-02-20
申请人： J.R. Simplot Company
发明人： Rommens, Caius M. T. , Ye, Jingsong , Yan, Hua , Swords, Kathleen M. , Menendez-Humara, Jaime , Brinkerhoff, Leigh W. , Richael, Craig
IPC分类号： C12N15/82 , C12N9/10 , A23L1/217 , C12N9/12
CPC分类号： C12N15/8202 , A01H1/00 , A01H1/04 , A23L5/11 , A23L19/12 , A23L19/18 , C07K14/415 , C12N9/0059 , C12N9/1051 , C12N9/1294 , C12N15/8205 , C12N15/8209 , C12N15/821 , C12N15/8216 , C12N15/8218 , C12N15/8226 , C12N15/8243 , C12N15/8245 , C12N15/825 , C12N15/8273
摘要： The present invention relates to a new plant breeding process. The process improves the agronomic performance of crop plants by using genetic material that is also used in classical breeding. Instead of sexually recombining entire genomes at random, as is done in classical breeding, specific genetic elements are rearranged in vitro and inserted back into individual plant cells. Plants obtained through this new plant breeding process do not contain foreign nucleic acid but only contain nucleic acid from the plant species selected for transformation or plants that are sexually compatible with the selected plant species. Plants developed through this new plant breeding process are provided. In particular, potato plants displaying improved tuber storage and health characteristics are provided.
摘要翻译：本发明涉及新的植物育种过程。该过程通过使用也用于古典育种的遗传物质改善作物的农艺性能。不同于随机地对整个基因组进行性重组，如在经典育种中所做的那样，特异性遗传元件在体外重新排列并插入到单独的植物细胞中。通过这种新的植物育种过程获得的植物不含外来核酸，但仅含有选自用于转化的植物物种的核酸或与所选择的植物物种性相容的植物。提供了通过这种新的植物育种过程开发的植物。特别地，提供显示改善的块茎储存和健康特征的马铃薯植物。

9. 发明公开

EP2235170A1 LIPID METABOLISM PROTEIN AND USES THEREOF III (PYRUVATE-ORTHOPHOSPHATE-DIKINASE) 审中-公开
标题翻译：脂质代谢蛋白及其用途III（丙酮酸磷酸二激酶）
公开(公告)号：EP2235170A1
公开(公告)日：2010-10-06
申请号：EP08861575.2
申请日：2008-12-17
申请人： BASF Plant Science GmbH
发明人： ZANK, Thorsten , HÄRTEL, Heiko, A.
IPC分类号： C12N9/12 , C12N15/82
CPC分类号： C12N15/8247 , C12N9/1294
摘要： The present invention relates to pyruvate-orthophosphate dikinase encoding polynucleotides and polypeptides. Further, the present invention relates to the use of the aforementioned nucleic acid sequences and proteins in transgenic plants. In particular, the invention is directed to methods for manipulating sugar-related compounds and for increasing oil level and altering the fatty acid composition in plants and seeds. The invention further relates to methods of using these novel plant polypeptides to stimulate plant growth and/or to increase yield and/or composition of seed storage compounds.

10. 发明授权

EP2222855B1 PLANTS WITH MODIFIED STARCH METABOLISM 有权
公开(公告)号：EP2222855B1
公开(公告)日：2018-08-01
申请号：EP08853342.7
申请日：2008-11-27
申请人： Commonwealth Scientific and Industrial Research Organisation
发明人： RAL, Jean-Philippe Francois Michel , LI, Zhongyi , MORELL, Matthew Kennedy
IPC分类号： C12N15/82 , C12N9/00 , C12N15/29 , A01H5/00 , C12N9/12
CPC分类号： C12N15/8245 , C07K14/415 , C12N9/1294 , C12N15/8234 , C12N15/8261 , C12Y207/09004
摘要： The specification provides methods of obtaining a genetically modified plant which has increased production potential compared to a control plant, the method comprising the steps of i) obtaining a plurality of plants at least one of which comprises in its genome a heterologous polynucleotide, ii) identifying from the plurality of plants a plant which has increased production potential relative to the control plant and comprises the heterologous polynucleotide, and iii) selecting the genetically modified plant, wherein the polynucleotide comprises a transcriptional control sequence operably linked to a nucleic acid sequence which encodes an agent that modifies endogenous starch phosphorylation and/or starch degradation in the plant. In some embodiments, the plant has increased endogenous glycosylase or increased digestibility compared to a control plant. In some specific embodiments, the endogenous starch phosphorylation and/or starch degradation is modified by modifying expression or activity of one or more enzymes selected from the group consisting of α-amylase (EC 3.2.1.1), &bgr;-amylase (EC 3.2.1.2), glucoamylase (EC 3.2.1.3), starch phosphorylase (EC2.4.1.1), glycosylase (EC 3.1.33), sucrase-isomaltase (EC 3.2.10), amylomaltase (EC 2.4.1.25), maltase (EC 3.2.1.20), isoamylase, and α-glucan, water dikinase (GWD, EC 2.7.9.4).

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