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1. 发明公开

EP0690128A1 POLYPEPTIDE HAVING COLD-RESISTANT PYRUVATE PHOSPHATE DIKINASE ACTIVITY, DNA CODING FOR THE SAME, AND RECOMBINANT VECTOR AND TRANSFORMED PLANT BOTH CONTAINING SAID DNA 失效
标题翻译：具有耐寒PYRUVATPHOSPHATDIKINASE活动中，DNA编码该重组VAKTOR多肽转化植物这两包括DNA
公开(公告)号：EP0690128A1
公开(公告)日：1996-01-03
申请号：EP95902286.4
申请日：1994-12-01
申请人： Japan Tobacco Inc.
发明人： OHTA, Shozo , USAMI, Satoru , BURNELL, James Nigel, Dep. of Molecular Sciences,
IPC分类号： C12N15/54 , C12N9/12 , C12N5/00
CPC分类号： C12N9/1294 , C12N15/8273
摘要： A novel polypeptide having a cold-resistant pyruvate phosphate dikinase activity; a cloned DNA coding for the polypeptide; and a recombinant vector containing the DNA, each as a means for imparting cold resistance to plants. The polypeptide is either the following polypeptide (1) or polypeptide (2) and has such an amino acid sequence that at least one amino acid residue present in the region having a length of one-sixth of the whole length starting with the C-terminus has been replaced by another amino acid residue and also has a cold-resistant pyruvate phosphate dikinase activity: 1) a polypeptide having an amino acid sequence represented by any of the SEQ ID no:1 to no:4 in the sequence listing and also having a pyruvate phosphate dikinase activity; and 2) a polypeptide having such an amino acid sequence that at least 50 % thereof is homologous with the amino acid sequence mentioned in item 1) and also having a pyruvate phosphate dikinase activity.
摘要翻译：具有耐寒丙酮酸磷酸二激酶活性的新的多肽; 克隆的DNA编码的多肽; 和含有该DNA，各作为一种手段用于赋予植物抗寒性的重组载体。所述多肽是下列任一多肽（1）或多肽（2）和在氨基酸序列已经审查做至少一个氨基酸残基存在于具有开始与C末端的整个长度的六分之一的长度的区域已取代另一个氨基酸残基，并因此具有耐寒丙酮酸磷酸二激酶活性：1）具有由任何SEQ ID没有表示的氨基酸序列的多肽：1至NO：清单4的序列中，因此具有丙酮酸磷酸二激酶活性; 和2）在氨基酸序列经审查的多肽做其至少50％同源，在第1项），并因此具有丙酮酸磷酸二激酶活性所提到的氨基酸序列。

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