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1. 发明公开

EP2298924A2 Improved method for the recovery of non-segmented, negative-stranded RNA viruses from cDNA 审中-公开
标题翻译： Verbittenes Verfahren zur Gewinnung nichtsegmentierter negativ-strang-RNA-Viren aus cDNA
公开(公告)号：EP2298924A2
公开(公告)日：2011-03-23
申请号：EP10174369.8
申请日：2004-06-08
申请人： Wyeth LLC
发明人： Parks, Christopher L. , Udem, Stephen A. , Sidhu, Modinderjit S.
IPC分类号： C12N15/86 , A61K39/12 , C12N7/00
CPC分类号： C07K14/005 , A61K39/00 , A61K48/00 , A61K2039/5254 , A61K2039/5256 , A61K2039/5258 , C12N7/00 , C12N15/86 , C12N2760/18022 , C12N2760/18422 , C12N2760/18451 , C12N2760/18452 , C12N2760/18551 , C12N2760/18552 , C12N2760/18622 , C12N2760/18651 , C12N2760/18652 , C12N2760/18662 , C12N2760/20243 , C12N2760/20251 , C12N2760/20252 , C12N2760/20262
摘要： Methods for producing infectious, non-segmented, negative-stranded RNA viruses of the Order Mononegavirales are provided that involve coexpression of a viral cDNA along with essential viral proteins, N, P, and L in a host cell transiently transfected with an expression vector encoding an RNA polymerase. In alternate methods, after the host cell is transfected with a viral cDNA expression vector and one or more vectors encoding the RNA polymerase, N protein, P protein, and L protein, the host cell is exposed to an effective heat shock under conditions sufficient to increase recovery of the recombinant virus. In other alternate embodiments, the host cells are transferred after viral rescue begins into co-culture with a plaque expansion cell, typically a monolayer of expansion cells, and the assembled infectious, non-segmented, negative-stranded RNA virus is recovered from the co-culture. Also provided within the invention are compositions for producing infectious, non-segmented, negative-stranded RNA virus of the Order Mononegavirales, recombinant viruses produced using the foregoing methods and compositions, and immunogenic compositions and methods employing the recombinant viruses. In additional embodiments, the methods and compositions of the invention are employed to produce growth- or replication-defective non-segmented negative-stranded RNA viruses and subviral particles.
摘要翻译：提供了用于产生单宁属病毒感染性，非分段的负链RNA病毒的方法，其涉及病毒cDNA与基本病毒蛋白N，P和L共表达在用表达载体编码的瞬时转染的宿主细胞中 RNA聚合酶。在替代方法中，在用病毒cDNA表达载体和编码RNA聚合酶，N蛋白，P蛋白和L蛋白的一种或多种载体转染宿主细胞后，将宿主细胞暴露于有效的热休克，增加重组病毒的恢复。在其他替代实施方案中，宿主细胞在病毒性拯救开始与斑块扩增细胞（通常为单层扩增细胞）共培养后被转移，并且组装的感染性，非分段的负链RNA病毒从共同 -文化。在本发明中还提供了用于产生单宁属（Mononegavirales）的感染性，非分段的负链RNA病毒的组合物，使用前述方法和组合物产生的重组病毒，以及使用重组病毒的免疫原性组合物和方法。在另外的实施方案中，本发明的方法和组合物用于产生生长或复制缺陷型非分段负链RNA病毒和亚病毒颗粒。

2. 发明公开

EP1633860A2 IMPROVED METHOD FOR THE RECOVERY OF NON-SEGMENTED, NEGATIVE-STRANDED RNA VIRUSES FROM cDNA 审中-公开
标题翻译：改进方法OBTAINING未分段负链RNA病毒从cDNA
公开(公告)号：EP1633860A2
公开(公告)日：2006-03-15
申请号：EP04754803.7
申请日：2004-06-08
申请人： Wyeth
发明人： PARKS, Christopher, L. , UDEM, Stephen, A. , SIDHU, Modinderjit, S.
IPC分类号： C12N7/00
CPC分类号： C07K14/005 , A61K39/00 , A61K48/00 , A61K2039/5254 , A61K2039/5256 , A61K2039/5258 , C12N7/00 , C12N15/86 , C12N2760/18022 , C12N2760/18422 , C12N2760/18451 , C12N2760/18452 , C12N2760/18551 , C12N2760/18552 , C12N2760/18622 , C12N2760/18651 , C12N2760/18652 , C12N2760/18662 , C12N2760/20243 , C12N2760/20251 , C12N2760/20252 , C12N2760/20262
摘要： Methods for producing infectious, non-segmented, negative-stranded RNA viruses of the Order Mononegavirales are provided that involve coexpression of a viral cDNA along with essential viral proteins, N, P, and L in a host cell transiently transfected with an expression vector encoding an RNA polymerase. In alternate methods, after the host cell is transfected with a viral cDNA expression vector and one or more vectors encoding the RNA polymerase, N protein, P protein, and L protein, the host cell is exposed to an effective heat shock under conditions sufficient to increase recovery of the recombinant virus. In other alternate embodiments, the host cells are transferred after viral rescue begins into co-culture with a plaque expansion cell, typically a monolayer of expansion cells, and the assembled infectious, non-segmented, negative-stranded RNA virus is recovered from the co-culture. Also provided within the invention are compositions for producing infectious, non-segmented, negative-stranded RNA virus of the Order Mononegavirales, recombinant viruses produced using the foregoing methods and compositions, and immunogenic compositions and methods employing the recombinant viruses. In additional embodiments, the methods and compositions of the invention are employed to produce growth- or replication-defective non-segmented negative-stranded RNA viruses and subviral particles.

3. 发明授权

EP2322611B1 METHOD FOR PRODUCTION OF REPROGRAMMED CELL USING CHROMOSOMALLY UNINTEGRATED VIRUS VECTOR 有权转让
标题翻译：使用染色体未整合的病毒载体产生重编程细胞的方法
公开(公告)号：EP2322611B1
公开(公告)日：2016-06-01
申请号：EP09797978.5
申请日：2009-07-16
申请人： ID Pharma Co., Ltd.
发明人： FUSAKI, Noemi , BAN, Hiroshi , HASEGAWA, Mamoru , YONEMITSU, Yoshikazu
IPC分类号： C07K14/47 , C12N5/074
CPC分类号： C12N15/86 , C07K14/4702 , C12N5/0696 , C12N7/00 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2510/00 , C12N2760/18622 , C12N2760/18643 , C12N2760/18645 , C12N2760/18662 , C12N2760/18822 , C12N2760/18843

4. 发明公开

EP3075850A1 METHOD FOR PRODUCTION OF REPROGRAMMED CELL USING CHROMOSOMALLY UNINTEGRATED VIRUS VECTOR 有权转让
标题翻译： VERFAHREN ZUR HERSTELLUNG EINER NEUPROGRAMMIERTEN ZELLE ANHAND EINES CHROMOSOMAL UNINTEGRIERTEN VIRUSVEKTORS
公开(公告)号：EP3075850A1
公开(公告)日：2016-10-05
申请号：EP16161002.7
申请日：2009-07-16
申请人： ID Pharma Co., Ltd.
发明人： Fusaki, Noemi , Ban, Hiroshi , Hasegawa, Mamoru , Yonemitsu, Yoshikazu
IPC分类号： C12N15/09 , C12N5/10 , C12N5/074 , C07K14/47 , C12N15/86
CPC分类号： C12N15/86 , C07K14/4702 , C12N5/0696 , C12N7/00 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2510/00 , C12N2760/18622 , C12N2760/18643 , C12N2760/18645 , C12N2760/18662 , C12N2760/18822 , C12N2760/18843
摘要： Disclosed is a vector for producing an ES-like cell having no foreign gene integrated into the chromosome thereof conveniently and efficiently. Also disclosed is a method for producing an ES-like cell from a somatic cell by using a chromosomally unintegrated virus vector. The ES-like cell produced by the method has no foreign gene integrated into the chromosome thereof. Therefore, the cell can be advantageously used for tests and studies, and has high possibility of eliminating the problem of immunological rejection or ethical problems in the treatment of diseases.
摘要翻译：公开了一种用于生产没有外来基因的ES样细胞方便有效地整合到染色体中的载体。还公开了通过使用染色体未整合的病毒载体从体细胞产生ES样细胞的方法。通过该方法产生的ES样细胞没有外源基因整合到其染色体中。因此，细胞可以有利地用于测试和研究，并且具有消除疾病治疗中的免疫排斥或伦理问题的高可能性。

5. 发明公开

EP2322611A4 METHOD FOR PRODUCTION OF REPROGRAMMED CELL USING CHROMOSOMALLY UNINTEGRATED VIRUS VECTOR 有权转让
标题翻译：制造方法重新编程小区是基于染色体非整合病毒载体
公开(公告)号：EP2322611A4
公开(公告)日：2012-04-18
申请号：EP09797978
申请日：2009-07-16
申请人： DNAVEC CORP
发明人： FUSAKI NOEMI , BAN HIROSHI , HASEGAWA MAMORU , YONEMITSU YOSHIKAZU
IPC分类号： C12N15/09 , C12N5/074 , C12N5/10
CPC分类号： C12N15/86 , C07K14/4702 , C12N5/0696 , C12N7/00 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2510/00 , C12N2760/18622 , C12N2760/18643 , C12N2760/18645 , C12N2760/18662 , C12N2760/18822 , C12N2760/18843

6. 发明公开

EP2322611A1 METHOD FOR PRODUCTION OF REPROGRAMMED CELL USING CHROMOSOMALLY UNINTEGRATED VIRUS VECTOR 有权转让
标题翻译： VERFAHREN ZUR HERSTELLUNG EINER NEUPROGRAMMIERTEN ZELLE ANHAND EINES CHROMOSOMAL UNINTEGRIERTEN VIRUSVEKTORS
公开(公告)号：EP2322611A1
公开(公告)日：2011-05-18
申请号：EP09797978.5
申请日：2009-07-16
申请人： Dnavec Corporation
发明人： FUSAKI, Noemi , BAN, Hiroshi , HASEGAWA, Mamoru , YONEMITSU, Yoshikazu
IPC分类号： C12N15/09 , C12N5/10
CPC分类号： C12N15/86 , C07K14/4702 , C12N5/0696 , C12N7/00 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2510/00 , C12N2760/18622 , C12N2760/18643 , C12N2760/18645 , C12N2760/18662 , C12N2760/18822 , C12N2760/18843
摘要： An objective of the present invention is to provide vectors for conveniently and efficiently producing ES-like cells in which foreign genes are not integrated into the chromosome. The present inventors discovered methods for producing ES-like cells from somatic cells using chromosomally non-integrating viral vectors. Since no foreign gene is integrated into the chromosome of the produced ES-like cells, they are advantageous in tests and research, and immunological rejection and ethical problems can be avoided in disease treatments.
摘要翻译：本发明的目的是提供用于方便和有效地产生外源基因未整合到染色体中的ES样细胞的载体。本发明人发现使用染色体非整合型病毒载体从体细胞产生ES样细胞的方法。由于没有外源基因整合到产生的ES样细胞的染色体中，因此在测试和研究中是有利的，并且在疾病治疗中可以避免免疫排斥和伦理问题。

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