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1. 发明公开

EP2899267A1 IN VITRO PREPARATION METHOD OF ROTAVIRUS DOUBLE-LAYER VIRUS-LIKE PARTICLES 有权
标题翻译： VITRO-VERFAHREN ZUR HERSTELLUNG VON ROTAVIRUS-DOPPELSCHICHTIGENVIRUSÄHNLICHENPARTIKELN
公开(公告)号：EP2899267A1
公开(公告)日：2015-07-29
申请号：EP13838213.0
申请日：2013-06-24
申请人： Xiamen University , Xiamen Innovax Biotech Co., Ltd.
发明人： GE, Shengxiang , LI, Tingdong , GUO, Qingshun , XU, Feihai , ZHANG, Jun , XIA, Ningshao
IPC分类号： C12N7/04 , C12P21/02 , C07K1/16 , C07K1/14 , C12R1/19
CPC分类号： C12N7/00 , A61K38/162 , A61K39/00 , C12N7/04 , C12N2720/12322 , C12N2720/12323 , C12N2720/12333 , C12N2720/12334 , C12N2720/12352
摘要： The invention relates to a method for preparing double-layered virus-like particles of rotavirus in vitro. The method comprises the following steps: purifying rotavirus VP6 proteins from a lysis supernatant, and in vitro assembling double-layered virus-like particles consisting of VP2 proteins and VP6 proteins, wherein the proteins and the virus-like particles can be used for preventing or reducing the clinical symptoms caused by rotavirus infection.
摘要翻译：本发明涉及体外制备轮状病毒双层病毒样颗粒的方法。该方法包括以下步骤：从裂解上清液中纯化轮状病毒VP6蛋白，以及体外组装由VP2蛋白和VP6蛋白组成的双层病毒样颗粒，其中蛋白质和病毒样颗粒可用于预防或减少轮状病毒感染引起的临床症状。

2. 发明授权

EP1583772B1 EMPLOYMENT OF ROTAVIRUS PROTEINS, DERIVED PROTEINS AND PEPTIDES FOR THE MODULATION OF TISSUE PERMEABILITY 有权
标题翻译：轮状病毒蛋白应用导出调制组织渗透性由其和蛋白质肽
公开(公告)号：EP1583772B1
公开(公告)日：2015-04-01
申请号：EP03700188.0
申请日：2003-01-10
申请人： Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional
发明人： GONZALEZ-MARISCAL, Lorenza , NAVA-DOMINGUEZ, Porfirio
IPC分类号： C07K14/14
CPC分类号： C07K14/005 , A61K38/162 , A61K47/42 , C07K2319/00 , C12N2720/12322 , Y02A50/471 , Y02A50/475 , Y02A50/483
摘要： The present invention refers to the use of rotavirus proteins VP4, VP8 and their derived fusion proteins and peptides, for enhancing the delivery of pharmaceutical agents through the paracellular pathway. These rotavirus proteins and derived peptides may additionally be employed to reduce unwanted cellular adhesion that can occur between cancerous cells, or between normal cells as a result of surgery, injury, chemotherapy, disease, inflammation or other pathological conditions.

3. 发明公开

EP2102344A2 PLASMID EXPRESSION VECTORS FOR EXPRESSION OF RECOMBINANT ROTAVIRUS AND ASTROVIRUS PROTEINS OR EPITOPES AND PRODUCTION ROCESS OF RAW MATERIAL 审中-公开
标题翻译：为原材料的重组轮状病毒和ASTRO病毒蛋白或表位和方法表达质粒的表达载体
公开(公告)号：EP2102344A2
公开(公告)日：2009-09-23
申请号：EP06804584.8
申请日：2006-10-20
申请人： FUNDACAO OSWALDO CRUZ (FIOCRUZ)
发明人： GÓES, Ana Carolina Magalhães , LEITE, José Paulo Gagliardi , DE MORAES SOUZA, Márcia Tereyinha Baroni , ARAUJO, Irene Trigueiros , D'HALLUIN, Jean, Claude , DA SILVA JR., Jose Godinho
IPC分类号： C12N15/40 , C12N15/46
CPC分类号： C07K14/005 , C12N2720/12322 , C12N2770/12022 , G01N33/56983 , G01N2333/14
摘要： The present invention refers to the production of specific recombinant viral proteins intended for use in the construction of a diagnostic kit for the simultaneous detection of the two most important gastroenteric viruses, namely rotavirus and astrovirus.

4. 发明公开

EP1478657A2 SEQUENCE ANALYSIS OF THE TETRAVALENT ROTAVIRUS VACCINE 审中-公开
标题翻译：四价轮状病毒疫苗序列分析
公开(公告)号：EP1478657A2
公开(公告)日：2004-11-24
申请号：EP03709213.7
申请日：2003-02-19
申请人： THE UNITED STATES GOVERNMENT,as represented by THE DEPARTMENT OF HEALTHAND HUMAN SERVICES
发明人： BUONAGURIO, Deborah, A. , GEORGIU, Alice, F. , LERCH, Robert, A. , MASON, Bruce, B. , MURTHY, Shridhara, C. , RAPPAPORT, Ruth, S. , SIDHU, Mohinder, S. , UDEM, Stephen, A. , ZAMB, Timothy, J.
IPC分类号： C07H21/04 , C12N15/74 , C12P21/06
CPC分类号： C12N7/00 , A61K39/00 , A61K2039/525 , A61K2039/5254 , C07K14/005 , C12N2720/12322 , C12N2720/12362 , C12Q1/701
摘要： Isolated nucleic acid molecules comprising a gene segment from a rhesus rotavirus (RRV) or from one of three rhesus: human reassortant viruses are disclosed, including isolated nucleic acid molecules having a sequence selected from the group consisting of: SEQ ID NO: 1-14, inclusive, and isolated nucleic acid molecules encoding a protein having a sequence selected from the group consisting of SEQ ID NO: 15-28, inclusive, as well as variants of the isolated nucleic acid molecules.

5. 发明公开

EP1170368A3 Immunization by inoculation of dna transcription unit 失效
标题翻译：免疫通过DNA转录单位的接种
公开(公告)号：EP1170368A3
公开(公告)日：2004-05-12
申请号：EP01202355.2
申请日：1995-01-25
申请人： UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER , ST. JUDE CHILDREN'S RESEARCH HOSPITAL
发明人： Robinson, Harriet L. , Fynan, Ellen F. , Webster, Robert G. , Lu, Shan
IPC分类号： C07K14/155 , C07K14/16 , C12N15/48 , A61K39/21
CPC分类号： C12N15/88 , A61K9/1652 , A61K39/00 , A61K39/12 , A61K39/145 , A61K39/15 , A61K39/21 , A61K48/00 , A61K2039/53 , A61K2039/54 , A61K2039/545 , C07K14/005 , C12N2720/12322 , C12N2720/12334 , C12N2740/11043 , C12N2740/15022 , C12N2740/15034 , C12N2740/16122 , C12N2760/16122 , C12N2760/16134
摘要： This invention relates to a method of immunizing a vertebrate, comprising introducing into the vertebrate a DNA transcription unit which comprises DNA encoding a desired antigen or antigens. The uptake of the DNA transcription unit by a host vertebrate results in the expression of the desired antigen or antigens, thereby eliciting humoral or cell-mediated immune responses or both humoral and cell-mediated responses. The elicited humoral and cell-mediated response can provide protection against invention by pathogenic agents, provide an anti-tumor response, or provide contraception. The host can be any vertebrate, avian or mammal, including humans.

6. 发明授权

EP0833670B1 VACCINE AGAINST ROTAVIRUS INFECTION COMPRISING PEPTIDES OF VIRAL ENTEROTOXIN NSP4 失效
标题翻译：轮状病毒疫苗感染VIRALENTEROTOXINES NSP4的含肽
公开(公告)号：EP0833670B1
公开(公告)日：2003-09-03
申请号：EP96923329.5
申请日：1996-06-14
申请人： BAYLOR COLLEGE OF MEDICINE
发明人： ESTES, Mary, K. , BALL, Judith , TIANG, Peng
IPC分类号： A61K38/16 , A61K39/15 , A61P43/00
CPC分类号： C07K14/005 , A61K38/00 , A61K39/00 , A61K49/0004 , C07K16/10 , C12N2720/12322
摘要： The rotavirus nonstructural glycoprotein, NSP4, performs multiple functions in the virus replication cycle, especially during viral morphogenesis. Specifically, NSP4 is an intracellular receptor that mediates the acquisition of a transient membrane envelope during the budding of newly formed subviral particles into the endoplasmic reticulum (ER). We now report that NSP4 alone induces diarrhea in young (6-11 days) rodents (mice and rats) when administered by the intraperitoneal (IP) or intraileal (IL) route. A 22 amino acid synthetic peptide corresponding to NSP4 residues 114-135 (NSP4 114-135) also induces diarrheal disease. Disease induction by the protein or peptide is specific and is both age and dose-dependent, and it occurs in the absence of virus replication. No diarrhea was observed with control proteins or peptides administered to the same age animals and by the same route. Electrophysiologic data from intestinal mucosa indicate that NSP4 and the peptide stimulate a calcium-mediated rise in chloride secretion, i.e., potentiate chloride secretion by a calcium dependent signaling pathway. Based on these results, a new mechanism by which rotavirus induces diarrhea is proposed. This model involves two intestinal receptors. One receptor is needed to permit viral infection to be initiated, while a second age-dependent receptor is needed to interact with NSP4, a noncapsid viral protein, expressed and released from viral-infected cells. Induction of a signal transduction pathway following the interaction of NSP4 (a viral enterotoxin) with the second receptor leads to chloride secretion and diarrhea, i.e., diarrhea is induced when NSP4, acting as a viral enterotoxin, triggers a signal transduction pathway. The action of this viral enterotoxin with its specific receptor mimics that of bacterial enterotoxins.

7. 发明授权

EP0672157B1 RECOMBINANT VIRUSES COMPRISING ARTIFICIAL PROTEOLYTIC CLEAVAGE SITE 失效
标题翻译：含有人工蛋白水解分裂重组病毒。
公开(公告)号：EP0672157B1
公开(公告)日：2001-10-10
申请号：EP93900925.4
申请日：1992-12-04
申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , AMERICAN CYANAMID COMPANY
发明人： FEINBERG, Mark , ANDINO, Raul , WEEKS-LEVY, Carolyn, Louise , REILLY, Patricia, Anne
IPC分类号： C12N15/86 , C12N7/01 , C12N15/38 , C12N15/43 , C12N15/44 , C12N15/46 , A61K39/106 , A61K39/245
CPC分类号： C07K14/005 , A61K39/00 , C07K14/235 , C07K14/28 , C07K2319/40 , C07K2319/50 , C07K2319/55 , C12N15/86 , C12N2710/16622 , C12N2720/12322 , C12N2730/10122 , C12N2760/16122 , C12N2770/32622 , Y02A50/466
摘要： Replication-competent recombinant viruses, particularly replication-competent recombinant polioviruses, which include (1) exogenous nucleic acid sequences which encode an exogenous polypeptide and (2) a nucleic acid sequence which encodes an artificial proteolytic cleavage site for a viral or cellular protease which proteolytically processes (cleaves) the precursor protein produced by the parent virus and uses therefor. The recombinant precursor is cleaved into the usual array of constituent proteins, freeing the exogenous polypeptide. Replication-competent recombinant viruses are useful as vaccines against bacterial, viral, fungal and yeast infections, parasitic diseases, cancer and allergies.

8. 发明公开

EP1127134A1 ROTAVIRUS SUBUNIT VACCINE 审中-公开
标题翻译：亚单位疫苗轮状病毒
公开(公告)号：EP1127134A1
公开(公告)日：2001-08-29
申请号：EP99956868.6
申请日：1999-11-01
申请人： CHILDREN'S HOSPITAL MEDICAL CENTER
发明人： CHOI, Anthony , WARD, Richard, L.
IPC分类号： C12N15/46 , C12N15/62 , C07K14/14 , C07K19/00 , A61K39/15
CPC分类号： C07K14/005 , A61K39/00 , C07K2319/00 , C12N2720/12322 , Y02A50/472
摘要： The present invention is directed to the generation and use of recombinant rotavirus fusion proteins as immunogens to produce a protective immune response from immunized individuals. In one embodiment, the present invention contemplates a recombinant rotavirus fusion protein vaccine composition comprising a rotavirus subunit protein or immunogenic fragment thereof, and an adjuvant in combination with the recombinant rotavirus subunit fusion protein. In one aspect of this embodiment, the recombinant rotavirus fusion protein comprises a rotavirus subunit protein and a fusion partner protein in genetic association with the rotavirus subunit protein, wherein the fusion partner protein does not interfere with expression and immunogenicity of the rotavirus subunit protein, the fusion partner protein prevents complex formation by the rotavirus subunit protein, and the fusion partner protein facilitates purification of the recombinant rotavirus fusion protein. In another aspect of this embodiment, the rotavirus subunit protein is selected from the group consisting of VP1, VP2, VP3, VP4, VP6, VP7, NSP1, NSP2, NSP3, NSP4 or NSP5. In yet another aspect of this embodiment, the rotavirus subunit protein is VP6.

9. 发明授权

EP0821583B1 DNA VACCINES AGAINST ROTAVIRUS INFECTIONS 失效
标题翻译： DNA疫苗抗轮状病毒感染
公开(公告)号：EP0821583B1
公开(公告)日：2000-10-04
申请号：EP95927480.4
申请日：1995-07-26
申请人： UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER
发明人： HERMANN, John, E. , ROBINSON, Harriet, L. , FYNAN, Ellen, F.
IPC分类号： C12N15/46 , A61K39/15 , C12N15/86
CPC分类号： A61K31/711 , A61K9/1652 , A61K38/00 , A61K39/00 , A61K39/12 , A61K39/15 , A61K48/00 , A61K2039/53 , A61K2039/54 , A61K2039/58 , C07K14/005 , C12N15/87 , C12N2720/12322 , C12N2720/12334 , C12N2740/15022 , C12N2740/16122 , C12N2760/16122
摘要： This invention relates to DNA vaccines for eliciting an immune response and/or protective immunity in a vertebrate by introducing into the vertebrate the DNA vaccine which consists essentially of DNA encoding an antigen or antigens, e.g. capsid proteins or polypeptides, of rotavirus. The uptake of the DNA vaccine by a host vertebrate results in the expression of the capsid protein, thereby eliciting humoral or cell-mediated immune responses, or both, which can provide protection against infection and/or prevent clinically significant rotavirus-caused disease. In addition, the invention demonstrates that an internal viral antigen provides protective immunity in a host. The host can be any vertebrate, including birds, piglets, and humans.

10. 发明公开

EP0833670A2 ROTAVIRUS ENTEROTOXIN NSP4 AND METHODS OF USING SAME 失效
标题翻译： NSP4轮状病毒肠毒素及其制备方法
公开(公告)号：EP0833670A2
公开(公告)日：1998-04-08
申请号：EP96923329.0
申请日：1996-06-14
申请人： BAYLOR COLLEGE OF MEDICINE
发明人： ESTES, Mary, K. , BALL, Judith , TIANG, Peng
IPC分类号： C12N15 , A61K39 , A61K45 , A61K49 , A61P1 , A61P3 , A61P31 , C07K14 , C07K16 , C12N7 , C12P21 , C12Q1 , G01N33 , A61K38
CPC分类号： C07K14/005 , A61K38/00 , A61K39/00 , A61K49/0004 , C07K16/10 , C12N2720/12322
摘要： The rotavirus nonstructural glycoprotein, NSP4, performs multiple functions in the virus replication cycle, especially during viral morphogenesis. Specifically, NSP4 is an intracellular receptor that mediates the acquisition of a transient membrane envelope during the budding of newly formed subviral particles into the endoplasmic reticulum (ER). We now report that NSP4 alone induces diarrhea in young (6-11 days) rodents (mice and rats) when administered by the intraperitoneal (IP) or intraileal (IL) route. A 22 amino acid synthetic peptide corresponding to NSP4 residues 114-135 (NSP4 114-135) also induces diarrheal disease. Disease induction by the protein or peptide is specific and is both age and dose-dependent, and it occurs in the absence of virus replication. No diarrhea was observed with control proteins or peptides administered to the same age animals and by the same route. Electrophysiologic data from intestinal mucosa indicate that NSP4 and the peptide stimulate a calcium-mediated rise in chloride secretion, i.e., potentiate chloride secretion by a calcium dependent signaling pathway. Based on these results, a new mechanism by which rotavirus induces diarrhea is proposed. This model involves two intestinal receptors. One receptor is needed to permit viral infection to be initiated, while a second age-dependent receptor is needed to interact with NSP4, a noncapsid viral protein, expressed and released from viral-infected cells. Induction of a signal transduction pathway following the interaction of NSP4 (a viral enterotoxin) with the second receptor leads to chloride secretion and diarrhea, i.e., diarrhea is induced when NSP4, acting as a viral enterotoxin, triggers a signal transduction pathway. The action of this viral enterotoxin with its specific receptor mimics that of bacterial enterotoxins.

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