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1. 发明授权

EP2360275B1 OLIGONUCLEOTIDE IDENTIFIERS 有权
公开(公告)号：EP2360275B1
公开(公告)日：2018-03-28
申请号：EP10184188.0
申请日：2001-11-15
申请人： Minerva Biotechnologies Corporation
发明人： BAMDAD, Cynthia , BAMDAD, Shoshana
IPC分类号： C12Q1/68 , G01N33/50
CPC分类号： C40B30/04 , B82Y30/00 , C12N15/1055 , C12Q1/6804 , C12Q1/6834 , G01N33/532 , G01N33/58 , G01N33/6842 , G01N33/6845 , C12Q2565/201 , C12Q2563/179 , C12Q2563/131 , C12Q2563/149 , C12Q2563/137
摘要： Methods, assays, and components are described in which biological samples can be rapidly and sensitively analyzed for the presence of species associated with neurodegenerative disease. Techniques and components are provided for diagnosis of disease, as well as for screening of candidate drugs for treatment of neurodegenerative disease. The techniques are simple, extremely sensitive, and utilize readily-available components. Binding species, capable of binding a neurodegenerative disease aggregate-forming or aggregate-forming species, are fastened to surfaces of electrodes and surfaces of particles, or provided free in solution, to bind aggregate-forming species and/or be involved in aggregation.

2. 发明授权

EP2078750B1 A fluorescent two-hybrid (F2H) assay for direct visualization of protein interactions in living cells 有权
标题翻译：荧光双杂交（F2H）测定法用于在活细胞中的蛋白质相互作用的直接可视化
公开(公告)号：EP2078750B1
公开(公告)日：2016-09-28
申请号：EP08000297.5
申请日：2008-01-09
申请人： Ludwig-Maximilians-Universität München
发明人： Rothbauer, Ulrich , Leonhardt, Heinrich , Zolghadr, Kourosh , Mortusewicz, Oliver
IPC分类号： C12N15/10 , G01N33/542 , C12Q1/68
CPC分类号： C12N15/1055 , C12N15/625 , G01N21/6486 , G01N33/542 , G01N33/6875 , G01N33/6884

3. 发明公开

EP2904093A4 METHODS OF QUANTITATING HEAVY AND LIGHT CHAIN POLYPEPTIDE PAIRS 有权转让
标题翻译： METHOD FOR重链和轻POLYPEPTIDPAAREN的定量测定
公开(公告)号：EP2904093A4
公开(公告)日：2016-06-08
申请号：EP13843363
申请日：2013-10-03
申请人： ZYMEWORKS INC , NAT RES COUNCIL OF CANADA NRC , BAARDSNES JASON , O'CONNOR-MCCOURT MAUREEN
发明人： BAARDSNES JASON , O'CONNOR-MCCOURT MAUREEN , OHRN ANDERS , CORPER ADAM LOUIS , NG GORDON YIU KON , SAMIOTAKIS ANTONIOS , CHOU YANG-CHIEH
IPC分类号： C12N5/07 , A61K39/00 , C12N5/16
CPC分类号： C12N15/1055 , A61K39/00 , C07K16/468 , C07K2317/55 , G01N21/6486
摘要： Provided is a method of quantitatively determining the ability of individual IgG heavy chains to selectively pair with a particular IgG light chain when the heavy chains and two unique light chains are co-expressed. The method provides results with reasonable throughput and is robust and accurate. The co-expressed heavy and light chains do not need to be isolated and purified which enables more efficient screening.

4. 发明公开

EP2847368A4 SURFACE ANCHORED LIGHT CHAIN BAIT ANTIBODY DISPLAY SYSTEM 有权转让
标题翻译：表面锚定的轻链诱饵抗体展示系统
公开(公告)号：EP2847368A4
公开(公告)日：2015-12-30
申请号：EP13787435
申请日：2013-05-06
申请人： MERCK SHARP & DOHME
发明人： SAHEEN HUSSAM HISHAM , ZHA DONGXING
IPC分类号： C40B30/04
CPC分类号： C12N15/1055 , C07K14/39 , C07K16/00 , C07K16/40 , C07K2317/41 , C07K2317/52 , C07K2317/56 , C07K2319/02 , C07K2319/035 , C40B40/02 , G01N33/6854 , G01N33/6857 , G01N2440/38 , C12Q2563/131
摘要： The present invention provides, in part, an antibody display system that simultaneously uses a secretion and a display mode. Embodiments of the invention provide a system in which a bait complexed with a monovalent antibody fragment can be captured prior to secretion in a host cell by virtue of surface displaying an antibody light chain and utilizing the covalent interaction of this light chain with the heavy chain of an antibody molecule that is co-expressed in the same host. Polypeptides, polynucleotides and host cells useful for making the antibody display system are also provided along with methods of using the system for identifying antibodies that bind specifically to an antigen of interest.

5. 发明公开

EP2622102A4 IN VIVO REPORTER SYSTEM 审中-公开
标题翻译：体内，REPORTERSYSTEM
公开(公告)号：EP2622102A4
公开(公告)日：2014-03-12
申请号：EP11829786
申请日：2011-09-26
申请人： RIGEL PHARMACEUTICALS INC
发明人： KINSELLA TODD M
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： G01N33/5061 , C07H21/04 , C07K2319/92 , C12N15/1055 , C12N15/79 , C12N2510/00 , C12N2800/00 , C12N2800/10 , C12Q1/6897
摘要： A construct system for expressing a reporter protein, as well as a transgenic animal and a screening method employing the same, are provided. In certain embodiments, the construct system is a three component system in which expression of a reporter protein from a reporter construct is induced by a transcription factor that is produced using two other constructs, each producing a different part of the transcription factor. The parts of the transcription factor are ligated together. Expression of the reporter only occurs in tissues in which both of the parts of the transcription factor are produced.
摘要翻译：提供了用于表达报道蛋白的构建体系，以及转基因动物和采用该构建体的筛选方法。在某些实施方案中，构建体系是三组分系统，其中来自报道构建体的报道蛋白的表达由使用两个其它构建体产生的转录因子诱导，每个构建体产生转录因子的不同部分。将转录因子的部分连接在一起。报告基因的表达仅发生在转录因子两部分产生的组织中。

6. 发明公开

EP2692865A1 Transposition-mediated identification of specific binding or functional proteins 有权
标题翻译：转座介导鉴定特异性结合或功能蛋白
公开(公告)号：EP2692865A1
公开(公告)日：2014-02-05
申请号：EP12178529.9
申请日：2012-07-30
申请人： NBE-Therapeutics LLC
发明人： Grawunder, Ulf
IPC分类号： C12N15/10 , C12N15/90
CPC分类号： C12N15/1055 , C07K16/28 , C07K2317/14 , C07K2317/55 , C07K2317/56 , C07K2317/622 , C12N5/10 , C12N9/1241 , C12N15/1082 , G01N33/6854 , C12N15/1037 , C12Q2525/179
摘要： The method disclosed herein describes a novel technology offering unparalleled efficiency, flexibility, utility and speed for the discovery and optimization of polypeptides having desired binding specificity and/or functionality, including antigen-binding molecules such as antibodies and fragments thereof, for desired functional and/or binding phenotypes. The novel method is based on transposable constructs and diverse DNA libraries cloned into transposable vectors and their transfection into host cells by concomitant transient expression of a functional transposase enzyme. This ensures an efficient, stable introduction of the transposon-based expression vectors into vertebrate host cells in one step, which can then be screened for a desired functional or binding phenotype of the expressed proteins, after which the relevant coding sequences for the expressed proteins, including antibodies and fragments thereof, can be identified by standard cloning and DNA sequencing techniques.
摘要翻译：本文公开的方法描述了提供无与伦比的效率，灵活性，实用性和速度的新技术，用于发现和优化具有期望的结合特异性和/或功能性的多肽，包括抗原结合分子如抗体及其片段，用于期望的功能性和/ 或结合表型。该新方法基于转座构建体和克隆到转座载体中的各种DNA文库以及它们通过功能性转座酶的伴随瞬时表达而转染入宿主细胞。这确保了一步有效，稳定地将基于转座子的表达载体导入脊椎动物宿主细胞中，然后可以筛选所表达的蛋白质的所需功能或结合表型，然后将表达蛋白质的相关编码序列包括抗体及其片段，可以通过标准克隆和DNA测序技术来鉴定。

7. 发明公开

EP2687599A1 Transposition-mediated identification of specific binding or functional proteins 审中-公开
标题翻译：特异性结合或功能性蛋白的换位介导的识别
公开(公告)号：EP2687599A1
公开(公告)日：2014-01-22
申请号：EP13177083.6
申请日：2013-07-18
申请人： NBE-Therapeutics LLC
发明人： Grawunder, Ulf
IPC分类号： C12N15/10 , C12N15/90
CPC分类号： C12N15/1055 , C07K16/28 , C07K2317/14 , C07K2317/55 , C07K2317/56 , C07K2317/622 , C12N5/10 , C12N9/1241 , C12N15/1082 , G01N33/6854 , C12N15/1037 , C12Q2525/179
摘要： The method disclosed herein describes a novel technology offering unparalleled efficiency, flexibility, utility and speed for the discovery and optimization of polypeptides having desired binding specificity and/or functionality, including antigen-binding molecules such as antibodies and fragments thereof, for desired functional and/or binding phenotypes. The novel method is based on transposable constructs and diverse DNA libraries cloned into transposable vectors and their transfection into host cells by concomitant transient expression of a functional transposase enzyme. This ensures an efficient, stable introduction of the transposon-based expression vectors into vertebrate host cells in one step, which can then be screened for a desired functional or binding phenotype of the expressed proteins, after which the relevant coding sequences for the expressed proteins, including antibodies and fragments thereof, can be identified by standard cloning and DNA sequencing techniques.
摘要翻译：在游离缺失的方法，光盘描述了一种新技术，提供用于发现和多肽的优化无与伦比的效率，灵活性，效用和速度具有所需的结合特异性和/或功能，包括抗原结合分子：例如抗体和其片段，对于期望的功能和/ 或结合的表型。该新方法是基于转座构建体，并克隆到载体转座及其转染到宿主细胞中由功能的转座酶的伴随瞬时表达各种DNA文库。这确保对高效，稳定引入基于转座子的表达载体引入脊椎动物宿主细胞在一个步骤中，其然后可以筛选过表达的蛋白质的期望的功能或结合表型，对于afterwhich过表达的蛋白质相关的编码序列，包括抗体和其片段，可以通过标准克隆和DNA测序技术来鉴定。

8. 发明公开

EP2622102A1 IN VIVO REPORTER SYSTEM 审中-公开
标题翻译：体内，REPORTERSYSTEM
公开(公告)号：EP2622102A1
公开(公告)日：2013-08-07
申请号：EP11829786.0
申请日：2011-09-26
申请人： Rigel Pharmaceuticals, Inc.
发明人： KINSELLA, Todd, M.
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： G01N33/5061 , C07H21/04 , C07K2319/92 , C12N15/1055 , C12N15/79 , C12N2510/00 , C12N2800/00 , C12N2800/10 , C12Q1/6897
摘要： A construct system for expressing a reporter protein, as well as a transgenic animal and a screening method employing the same, are provided. In certain embodiments, the construct system is a three component system in which expression of a reporter protein from a reporter construct is induced by a transcription factor that is produced using two other constructs, each producing a different part of the transcription factor. The parts of the transcription factor are ligated together. Expression of the reporter only occurs in tissues in which both of the parts of the transcription factor are produced.

9. 发明授权

EP1166121B1 SELECTION OF INTRACELLULAR IMMUNOGLOBULINS 有权
标题翻译：选择INTRA蜂窝免疫球蛋白
公开(公告)号：EP1166121B1
公开(公告)日：2011-12-14
申请号：EP00909465.7
申请日：2000-03-10
申请人： Medical Research Council , Scuola Internazionale Superiore di Studi Avanzati
发明人： TSE, Eric MRC Lab.of Molecular Biology , RABBITS, Terence MRC Lab.of Molecular Biology , CATTANEO, Antonio, SISSA , VISINTIN, Michela, SISSA
IPC分类号： G01N33/68 , C12N15/10 , C12Q1/68
CPC分类号： C07K16/32 , C07K16/08 , C07K16/40 , C07K2317/622 , C07K2319/00 , C12N15/1055 , G01N33/6854 , Y10T436/13
摘要： A general immunoglobulin-target assay system is provided, in which a positive outcome (the generation of a signal) depends only on the intracellular interaction of immunoglobulin with target. This can be accomplished for many immunoglobulins expressed in yeast and/or in mammalian cells and allows the selection of immunoglobulins which are capable of functioning in an intracellular environment.

10. 发明公开

EP2392596A2 Intrabodies with defined framework that is stable in a reducing environment and applications thereof 审中-公开
标题翻译：用限定的结构（“框架”），其是稳定在还原环境中，并且它们的用途胞内抗体
公开(公告)号：EP2392596A2
公开(公告)日：2011-12-07
申请号：EP10010030.4
申请日：2000-12-18
申请人： ESBATech, an Alcon Biomedical Research Unit LLC
发明人： Auf der Maur, Adrian , Barberis, Alcide , Escher, Dominik
IPC分类号： C07K16/00 , C12N15/10 , C12N15/13 , A61K39/395 , C07K19/00 , G01N33/577
CPC分类号： C07K16/005 , A61K2039/505 , C07K16/00 , C07K16/14 , C07K2317/622 , C07K2317/80 , C07K2318/10 , C07K2319/00 , C12N15/1055 , C12N15/1093
摘要： Methods for the generation of a CDR library with a defined framework that is stable and soluble in a reducing environment are described as well as thus obtained library of intrabodies.
摘要翻译：对于CDR库定义的框架的生成方法所做的就是稳定，易溶于还原环境中被描述，以及细胞内抗体由此获得库。

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