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1. 发明公开

EP2777714A1 Method of producing an immunoligand/payload conjugate by means of a sequence-specific transpeptidase enzyme 审中-公开
标题翻译：一种用于通过序列特异性转酶来生产immunoligand / Nutzlastkonjugats方法
公开(公告)号：EP2777714A1
公开(公告)日：2014-09-17
申请号：EP13159484.8
申请日：2013-03-15
申请人： NBE-Therapeutics LLC
发明人： Grawunder, Ulf
IPC分类号： A61K47/48 , C07K1/107 , C07K16/18
CPC分类号： A61K47/65 , A61K47/6803 , A61K47/6851 , A61K47/6889 , A61K2039/505 , C07K1/1075 , C07K16/40 , C07K2317/24 , C12P21/00 , C12Q2521/537
摘要： The present invention relates to a method of producing an immunoligand/payload conjugate, which method encompasses conjugating a payload to an immunoligand by means of a sequence-specific transpeptidase enzyme, or a catalytic domain thereof ( Fig. 2 ).
摘要翻译：本发明涉及到在immunoligand /有效载荷缀合物，所述方法包括通过序列特异性转酶的手段，在缀合一个immunoligand有效载荷，或催化结构域及其制造的方法（图2）。

2. 发明公开

EP2692865A1 Transposition-mediated identification of specific binding or functional proteins 有权
标题翻译：转座介导鉴定特异性结合或功能蛋白
公开(公告)号：EP2692865A1
公开(公告)日：2014-02-05
申请号：EP12178529.9
申请日：2012-07-30
申请人： NBE-Therapeutics LLC
发明人： Grawunder, Ulf
IPC分类号： C12N15/10 , C12N15/90
CPC分类号： C12N15/1055 , C07K16/28 , C07K2317/14 , C07K2317/55 , C07K2317/56 , C07K2317/622 , C12N5/10 , C12N9/1241 , C12N15/1082 , G01N33/6854 , C12N15/1037 , C12Q2525/179
摘要： The method disclosed herein describes a novel technology offering unparalleled efficiency, flexibility, utility and speed for the discovery and optimization of polypeptides having desired binding specificity and/or functionality, including antigen-binding molecules such as antibodies and fragments thereof, for desired functional and/or binding phenotypes. The novel method is based on transposable constructs and diverse DNA libraries cloned into transposable vectors and their transfection into host cells by concomitant transient expression of a functional transposase enzyme. This ensures an efficient, stable introduction of the transposon-based expression vectors into vertebrate host cells in one step, which can then be screened for a desired functional or binding phenotype of the expressed proteins, after which the relevant coding sequences for the expressed proteins, including antibodies and fragments thereof, can be identified by standard cloning and DNA sequencing techniques.
摘要翻译：本文公开的方法描述了提供无与伦比的效率，灵活性，实用性和速度的新技术，用于发现和优化具有期望的结合特异性和/或功能性的多肽，包括抗原结合分子如抗体及其片段，用于期望的功能性和/ 或结合表型。该新方法基于转座构建体和克隆到转座载体中的各种DNA文库以及它们通过功能性转座酶的伴随瞬时表达而转染入宿主细胞。这确保了一步有效，稳定地将基于转座子的表达载体导入脊椎动物宿主细胞中，然后可以筛选所表达的蛋白质的所需功能或结合表型，然后将表达蛋白质的相关编码序列包括抗体及其片段，可以通过标准克隆和DNA测序技术来鉴定。

3. 发明公开

EP2687599A1 Transposition-mediated identification of specific binding or functional proteins 审中-公开
标题翻译：特异性结合或功能性蛋白的换位介导的识别
公开(公告)号：EP2687599A1
公开(公告)日：2014-01-22
申请号：EP13177083.6
申请日：2013-07-18
申请人： NBE-Therapeutics LLC
发明人： Grawunder, Ulf
IPC分类号： C12N15/10 , C12N15/90
CPC分类号： C12N15/1055 , C07K16/28 , C07K2317/14 , C07K2317/55 , C07K2317/56 , C07K2317/622 , C12N5/10 , C12N9/1241 , C12N15/1082 , G01N33/6854 , C12N15/1037 , C12Q2525/179
摘要： The method disclosed herein describes a novel technology offering unparalleled efficiency, flexibility, utility and speed for the discovery and optimization of polypeptides having desired binding specificity and/or functionality, including antigen-binding molecules such as antibodies and fragments thereof, for desired functional and/or binding phenotypes. The novel method is based on transposable constructs and diverse DNA libraries cloned into transposable vectors and their transfection into host cells by concomitant transient expression of a functional transposase enzyme. This ensures an efficient, stable introduction of the transposon-based expression vectors into vertebrate host cells in one step, which can then be screened for a desired functional or binding phenotype of the expressed proteins, after which the relevant coding sequences for the expressed proteins, including antibodies and fragments thereof, can be identified by standard cloning and DNA sequencing techniques.
摘要翻译：在游离缺失的方法，光盘描述了一种新技术，提供用于发现和多肽的优化无与伦比的效率，灵活性，效用和速度具有所需的结合特异性和/或功能，包括抗原结合分子：例如抗体和其片段，对于期望的功能和/ 或结合的表型。该新方法是基于转座构建体，并克隆到载体转座及其转染到宿主细胞中由功能的转座酶的伴随瞬时表达各种DNA文库。这确保对高效，稳定引入基于转座子的表达载体引入脊椎动物宿主细胞在一个步骤中，其然后可以筛选过表达的蛋白质的期望的功能或结合表型，对于afterwhich过表达的蛋白质相关的编码序列，包括抗体和其片段，可以通过标准克隆和DNA测序技术来鉴定。

4. 发明授权

EP2692865B1 Transposition-mediated identification of specific binding or functional proteins 有权
标题翻译：特异性结合和功能蛋白的换位介导的识别
公开(公告)号：EP2692865B1
公开(公告)日：2014-12-10
申请号：EP12178529.9
申请日：2012-07-30
申请人： NBE-Therapeutics LLC
发明人： Grawunder, Ulf
IPC分类号： C12N15/10 , C12N15/90
CPC分类号： C12N15/1055 , C07K16/28 , C07K2317/14 , C07K2317/55 , C07K2317/56 , C07K2317/622 , C12N5/10 , C12N9/1241 , C12N15/1082 , G01N33/6854 , C12N15/1037 , C12Q2525/179

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