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1. 发明授权

EP3031919B1 TAILORED MULTI-SITE COMBINATIONAL ASSEMBLY 有权转让
公开(公告)号：EP3031919B1
公开(公告)日：2018-09-19
申请号：EP16154206.3
申请日：2008-07-31
申请人： BASF Enzymes LLC
发明人： TAN, Xuqiu
IPC分类号： C12N15/10 , C12P19/34
CPC分类号： C12P19/34 , C12N15/102 , C12N15/1024
摘要： The present invention provides a novel method of producing a plurality of modified polynucleotides having different combinations of various mutations at multiple sites by a tailored multi-site combinatorial assembly, comprising adding at least two or at least three primers to a double stranded template polynucleotide in a single reaction mixture, wherein the primers are not overlapping, and wherein each of the primers comprise at least one mutation different from the other primers, wherein at least one primer is a forward primer that can anneal to a minus strand of the template and at least one primer is a reverse primer that can anneal to a plus strand of the template, and subjecting the reaction mixture to a polymerase extension reaction to yield a plurality of extended modified polynucleotides from the at least three primers. The method can be performed without employing a ligation step prior to transforming the extended modified polynucleotides into a cell. The plurality of extended modified polynucleotides can be treated with an enzyme for destroying the template polynucleotide prior to transforming in to the cell.

2. 发明公开

EP3216867A1 METHOD FOR MODIFYING GENOME SEQUENCE TO INTRODUCE SPECIFIC MUTATION TO TARGETED DNA SEQUENCE BY BASE-REMOVAL REACTION, AND MOLECULAR COMPLEX USED THEREIN 有权
标题翻译：通过碱基去除反应修饰基因组序列以引入靶向DNA序列的特定突变的方法及其中使用的分子复合物
公开(公告)号：EP3216867A1
公开(公告)日：2017-09-13
申请号：EP15857325.3
申请日：2015-11-02
申请人： National University Corporation Kobe University
发明人： NISHIDA, Keiji , KONDO, Akihiko
IPC分类号： C12N15/09 , C07K19/00 , C12N9/24
CPC分类号： C12N15/1024 , C07K19/00 , C12N9/22 , C12N9/24 , C12N9/2497 , C12N15/09 , C12N15/11 , C12N2310/20 , C12Y302/02027
摘要： The present invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and DNA glycosylase with sufficiently low reactivity with a DNA having an unrelaxed double helix structure (unrelaxed DNA) are bonded, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.
摘要翻译：本发明提供了一种修饰双链DNA的靶向位点的方法，该方法包括使复合体与选定的双链DNA中的靶核苷酸序列特异性结合的核酸序列识别模块和DNA糖基化酶与与具有非松弛双螺旋结构的DNA（非松弛DNA）的足够低的反应性与双链DNA结合以将靶位点中的一个或多个核苷酸转化为其他一个或多个核苷酸或删除一个或多个核苷酸或插入一个或多个核苷酸进入靶向位点，而不会切割靶向位点中双链DNA的至少一条链。

3. 发明公开

EP3116997A1 CRISPR/CAS-RELATED METHODS AND COMPOSITIONS FOR TREATING LEBER'S CONGENITAL AMAUROSIS 10 (LCA10) 有权转让
标题翻译： CRISPR / CAS相关方法和组合物治疗LEBER的先天性耳毛畸形10（LCA10）
公开(公告)号：EP3116997A1
公开(公告)日：2017-01-18
申请号：EP15714320.7
申请日：2015-03-10
申请人： Editas Medicine, Inc.
发明人： MAEDER, Morgan, L. , BUMCROT, David, A. , SHEN, Shen
IPC分类号： C12N15/113 , A61K31/7088 , C12N9/22 , C12N15/86 , A61K48/00
CPC分类号： C12N15/1024 , C12N9/22 , C12N15/11 , C12N15/113 , C12N15/86 , C12N2310/10 , C12N2310/20 , C12N2750/14132 , C12N2750/14142 , C12N2750/14143 , C12N2750/14145 , C12N2750/14151
摘要： CRISPR/CAS-related compositions and methods for treatment of Leber's Congenital Amaurosis 10 (LCA10) are disclosed.
摘要翻译：公开了CRISPR / CAS相关的组合物和用于治疗莱伯氏先天性黑麦草10（LCA10）的方法。

4. 发明公开

EP3097196A2 NEGATIVE SELECTION AND STRINGENCY MODULATION IN CONTINUOUS EVOLUTION SYSTEMS 有权
标题翻译：负选择而产生STRINGENZMODULATION不断发展中，系统
公开(公告)号：EP3097196A2
公开(公告)日：2016-11-30
申请号：EP15759185.0
申请日：2015-01-20
申请人： President and Fellows of Harvard College
发明人： LIU, David R. , CARLSON, Jacob Charles , BADRAN, Ahmed Hussein , ESVELT, Kevin Michael
IPC分类号： C12N15/63 , C12N15/01 , C12Q1/02 , C12N9/12
CPC分类号： C12N15/1058 , C12N7/00 , C12N15/01 , C12N15/1024 , C12N15/1037 , C12N15/64 , C12N15/70 , C12N2795/14121 , C12N2795/14152 , C40B10/00 , C40B50/06
摘要： Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and/or positive selection of desired properties.

5. 发明公开

EP2931891A4 RNA-GUIDED HUMAN GENOME ENGINEERING 有权
标题翻译： RNA-GESTEUERTES工程师EINES MENSCHLICHEN GENOMS
公开(公告)号：EP2931891A4
公开(公告)日：2016-07-13
申请号：EP13863815
申请日：2013-12-16
申请人： HARVARD COLLEGE
发明人： CHURCH GEORGE M , MALI PRASHANT , YANG LUHAN
IPC分类号： C12N15/00 , C12N9/22
CPC分类号： C12N15/85 , C12N9/22 , C12N15/01 , C12N15/10 , C12N15/102 , C12N15/1024 , C12N15/63 , C12N15/81 , C12N15/8201 , C12N15/87 , C12N15/90 , C12N15/907 , C12N2800/80 , C12N2810/55 , C12Y301/00
摘要： A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
摘要翻译：提供了改变真核细胞的方法，包括用编码与真核细胞的基因组DNA互补的RNA的核酸转染真核细胞，用编码与RNA相互作用并切割基因组DNA的酶的核酸转染真核细胞以位点特异性方式，其中细胞表达RNA和酶，RNA结合互补基因组DNA，酶以位点特异性方式切割基因组DNA。

6. 发明公开

EP2914728A4 METHODS AND PRODUCTS FOR EXPRESSING PROTEINS IN CELLS 有权
标题翻译：方法和产品的蛋白在细胞中的表达
公开(公告)号：EP2914728A4
公开(公告)日：2016-05-25
申请号：EP13850281
申请日：2013-11-01
申请人： FACTOR BIOSCIENCE INC
发明人： ANGEL MATTHEW , ROHDE CHRISTOPHER
IPC分类号： C12N15/31 , A61K31/7115 , A61K38/46 , A61K48/00 , A61P25/14 , A61P25/28 , A61P35/00 , C12N5/10 , C12N9/22 , C12N15/63 , C12Q1/68
CPC分类号： C12N15/907 , A61K31/7115 , A61K35/14 , A61K38/465 , A61K48/005 , A61K2035/124 , C07K14/195 , C07K2319/80 , C12N9/22 , C12N15/1024 , C12N15/86 , C12N2800/80 , C12Y301/00 , C12Y301/21004 , G01N33/5014
摘要： The present invention relates in part to nucleic acids encoding proteins, therapeutics comprising nucleic acids encoding proteins, methods for inducing cells to express proteins using nucleic acids, methods, kits and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods and products for altering the DNA sequence of a cell are described, as are methods and products for inducing cells to express proteins using synthetic RNA molecules. Therapeutics comprising nucleic acids encoding gene-editing proteins are also described.

7. 发明授权

EP2646550B1 TARGETED ALTERATION OF DNA WITH OLIGONUCLEOTIDES 有权
标题翻译：用寡核苷酸DNA的靶向修饰
公开(公告)号：EP2646550B1
公开(公告)日：2015-02-18
申请号：EP11791642.9
申请日：2011-11-25
申请人： Keygene N.V.
发明人： DE BOTH, Michiel Theodoor Jan , FURUKAWA, Tomoyuki
IPC分类号： C12N15/10
CPC分类号： C12N15/1024 , C12N15/102 , C12N15/1082
摘要： The current invention relates to a method for targeted alteration of acceptor DNA, for example duplex acceptor DNA. The method comprises use of at least two oligonucleotides, each oligonucleotide having at least one mismatch relative to the targeted (duplex) acceptor DNA. The mismatch of the first oligonucleotide is directed to a nucleotide at a position in the first strand of the duplex and the mismatch of the second oligonucleotide is directed to the nucleotide in the second strand that occupies the complementary position in the duplex acceptor DNA (e.g. forms a base-pair with the nucleotide in the first strand). These mismatches are located at specific positions within said oligonucleotides. Also provided is a kit that comprises instructions for performing the method according to the inventions, and in a preferred embodiment, comprises oligonucleotides suitable for use in the method.

8. 发明公开

EP2274421A1 CIS-ACTING DIVERSIFICATION ACTIVATOR AND METHOD FOR SELECTIVE DIVERSIFICATION OF NUCLEIC ACIDS 审中-公开
标题翻译：顺式作用DIVERSIFIKATIONSAKTIVATOR和方法对于核酸的选择性多样化
公开(公告)号：EP2274421A1
公开(公告)日：2011-01-19
申请号：EP09730911.6
申请日：2009-04-09
申请人： Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)
发明人： BUERSTEDDE, Jean-Marie , CALDWELL, Randolph, B. , SCHÖTZ, Ulrike
IPC分类号： C12N15/00 , C12N15/01 , C12N5/06
CPC分类号： C12N15/1024
摘要： The invention relates to identification of a
cis -acting diversification activator (
DIVAC ) that is necessary and sufficient for the activation of diversification in transcription units linked thereto. The invention provides a method for diversification of a target nucleic acid comprising introducing a genetic construct comprising the diversification activator into a recipient cell, wherein the diversification activator is linked to the target nucleic acid.

9. 发明授权

EP2110440B1 Regulated vectors for controlling DNA hypermutability in eukaryotic cells 有权
标题翻译：用于DNA超突变的真核细胞中控制调节的矢量
公开(公告)号：EP2110440B1
公开(公告)日：2010-12-22
申请号：EP09155285.1
申请日：2003-02-21
申请人： Morphotek, Inc.
发明人： Grasso, Luigi , Nicholaides, Nicholas C , Sass, Philip M
IPC分类号： C12Q1/68 , C12N15/85
CPC分类号： C12N15/1024 , C12N15/85 , C12N2830/42 , C12N2840/203

10. 发明授权

EP1551988B1 METHODS FOR GENERATING ENHANCED ANTIBODY-PRODUCING CELL LINES WITH IMPROVED GROWTH CHARACTERISTICS 有权
标题翻译：方法制备改善的抗体生产具有改进的生长特性细胞系
公开(公告)号：EP1551988B1
公开(公告)日：2010-11-24
申请号：EP03765839.0
申请日：2003-07-21
申请人： Morphotek, Inc.
发明人： GRASSO, Luigi , KLINE, J. Bradford , NICOLAIDES, Nicholas, C. , SASS, Philip, M.
IPC分类号： C12P21/00 , C12P21/04 , C12N15/00 , C12N15/63 , C12N15/87 , C12Q1/68 , C12Q1/70 , G01N33/53 , A61K48/00
CPC分类号： C12N15/1024 , C07K16/00 , C12N15/1034
摘要： The use of mismatch repair (MMR) defective antibody producer cells offers a method to generate subclone variants with elevated protein production such as antibodies. Using MMR defective cells and animals, new cell lines and animal varieties with novel and useful properties such as enhanced protein production can be generated more efficiently than by relying on the natural rate of mutation. These methods are useful for generating genetic diversity within host cells to alter endogenous genes that can yield increased titer levels of protein production. By employing this method, two genes were discovered whose suppressed expression is associated with enhanced antibody production. Suppressed expression of these genes by a variety of methods leads to increased antibody production for manufacturing as well as strategies for modulating antibody production in immunological disorders. Moreover, the suppression of these two genes in host cells can be useful for generating universal high titer protein production lines.

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