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1. 发明授权

EP2483198B1 DREIDIMENSIONALE MIKRO-STRUKTUR, ANORDNUNG MIT MINDESTENS ZWEI DREIDIMENSIONALEN MIKRO-STRUKTUREN, VERFAHREN ZUM HERSTELLEN DER MIKRO-STRUKTUR UND VERWENDUNG DER MIKRO-STRUKTUR 有权
标题翻译：至少有两个二维微结构，其制造方法微结构和使用微观结构的三维微结构安排
公开(公告)号：EP2483198B1
公开(公告)日：2016-11-02
申请号：EP10760290.6
申请日：2010-09-20
申请人： Siemens Aktiengesellschaft
发明人： HEDLER, Harry , ZAPF, Jörg
IPC分类号： B81C99/00
CPC分类号： B81C99/0085 , B81B2203/0361 , B81B2207/056 , Y10T29/49826 , Y10T428/24355

2. 发明公开

EP2888048A1 MULTIWELL PLATES COMPRISING NANOWIRES 审中-公开
标题翻译：与纳米线多孔板
公开(公告)号：EP2888048A1
公开(公告)日：2015-07-01
申请号：EP13717359.7
申请日：2013-03-15
申请人： President and Fellows of Harvard College
发明人： PARK, Hongkun , SHALEK, Alexander, K. , DING, Ruihua , PARK, Joseph
IPC分类号： B01L3/00 , B82Y5/00 , B82Y15/00 , C12M1/20
CPC分类号： B81C1/00111 , B01L3/5085 , B01L99/00 , B01L2300/0851 , B01L2300/0896 , B01L2300/163 , B01L2400/086 , B81B7/04 , B81B2201/055 , B81B2203/0361 , B81B2207/056 , B82Y15/00 , B82Y30/00 , C12M23/12 , C12M25/00 , C12N1/20 , C12N5/0068 , Y10T156/10
摘要： The present invention generally relates to nanowires and, in particular, to multiwell plates comprising nanowires, including systems and methods of making the same. Such multiwell plates can, in some cases, be used in automated equipment or high-throughput applications. For example, a plurality of cells may be placed in at least some of the wells of the multiwell plate, and one or more nanowires may be inserted into at least some of the cells within the wells of the multiwell plate. In some cases, one or more of the nanowires may have coated thereon a biological effector. The cells in each of the wells may be identical or different, and/or the biological effector may the same or different. Such multiwell plates may be used, for example, to test a biological effector against a variety of cell types, or to test a variety of biological effectors against a one or more cell types, or the like.

3. 发明公开

EP2888047A1 FABRICATION OF NANOWIRE ARRAYS 审中-公开
标题翻译：备纳米线阵列
公开(公告)号：EP2888047A1
公开(公告)日：2015-07-01
申请号：EP13715062.9
申请日：2013-03-15
申请人： President and Fellows of Harvard College
发明人： PARK, Hongkun , SHALEK, Alexander, K. , JORGOLLI, Marsela
IPC分类号： B01L3/00 , B82Y5/00 , B82Y15/00 , C12M1/20 , B81C1/00
CPC分类号： B81C1/00111 , B01L3/5085 , B01L99/00 , B01L2300/0851 , B01L2300/0896 , B01L2300/163 , B01L2400/086 , B81B7/04 , B81B2201/055 , B81B2203/0361 , B81B2207/056 , B82Y15/00 , B82Y30/00 , C12M23/12 , C12M25/00 , C12N1/20 , C12N5/0068 , Y10T156/10
摘要： The present invention generally relates to nanowire arrays and methods of fabricating such arrays. In certain embodiments, the fabrication methods can consistently form NW arrays with reproducible configurations at nanoscale sites and produce NWs of specified heights, diameters, and densities. In some cases, the methods also allow formation of NW arrays containing barriers between regions, which would be prohibitively expensive if prepared by other methods.

4. 发明公开

EP2650256A2 Verfahren zur Herstellung eines mikrostrukturierten Formkörpers 有权
标题翻译： Verfahren zur Herstellung eines mikrostrukturiertenFormkörpers
公开(公告)号：EP2650256A2
公开(公告)日：2013-10-16
申请号：EP13162904.0
申请日：2013-04-09
申请人： Technische Universität Ilmenau
发明人： Schober, Andreas , Hampl, Jörg , Weise, Frank , Schlingloff, Gregor , Fernekorn, Uta
IPC分类号： B81C1/00 , B81C99/00
CPC分类号： B44C1/227 , B81B2201/06 , B81B2203/0346 , B81B2207/056 , B81C99/0085 , B81C2201/034 , Y10T428/24281
摘要： Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung eines mikrostrukturierten Formkörpers, welcher insbesondere zur Kultivierung von biologischen Zellen vorgesehen ist. Gemäß diesem Verfahren wird zunächst eine plastisch verformbare, poröse erste Folie (01) bereitgestellt. Weiterhin werden eine verformbare zweite Folie (02) und eine verformbare Opferfolie (03) bereitgestellt. Die erste Folie (01), die zweite Folie (02) und die Opferfolie (03) werden zu einem Folienstapel (01, 02, 03) gestapelt. Anschließend wird die Opferfolie (03) mit einem Druck beaufschlagt, um den Folienstapel (01, 02 ,03) in eine Form (04) zu pressen. Die Form (04) weist Ausnehmungen (06) auf, wodurch in der Opferfolie (03), in der ersten Folie (01) und in der zweiten Folie (02) jeweils verformte Bereiche (07, 09) in Form von Kavitäten ausgebildet werden und unverformte Bereiche (08, 11) verbleiben. Beim Pressen des Folienstapels (01, 02, 03) in die Form (04) werden die erste Folie (01) und die zweite Folie (02) miteinander verbunden, sodass sie einen Folienverbund (01, 02) ausbilden. Daraufhin erfolgt ein Ätzen von zumindest Teilen der verformten Bereiche (09) der zweiten Folie (02), wodurch Abschnitte der zweiten Folie (02) chemisch aufgelöst werden. In diesen Abschnitten der zweiten Folie (02) werden Abschnitte (12) in den verformten Bereichen (07) der ersten Folie (01) freigelegt, sodass die Poren in diesen Abschnitten (12) wieder geöffnet werden.
摘要翻译：该方法包括堆叠具有孔的可变形的第一膜（01），可变形的第二膜（O 2）和可变形的第一牺牲膜以形成一叠膜，用压力将膜堆叠压在模具中其中变形部分以空腔形式形成，未变形区域保留在第一牺牲膜中，在第一膜和第二膜中，并且第一膜和第二膜（O 2）连接在一起以形成复合膜并从复合膜中除去第一受害膜。该方法包括堆叠具有孔的可变形的第一膜（01），可变形的第二膜（O 2）和可变形的第一牺牲膜以形成一叠膜，用压力将膜堆叠压在模具中其中变形部分以空腔形式形成，未变形区域保留在第一牺牲膜中，在第一膜和第二膜中，并且第一膜和第二膜（O 2）连接在一起以形成复合膜从复合膜去除第一受害膜，并且通过化学溶解部分以暴露第一膜中的变形部分（07）来蚀刻第二膜的变形部分的部分。第一膜和第二膜彼此连接。孔的直径为500〜2μm。第一片厚度为20-80微米。模具具有用于形成空腔的凹槽。凹槽的直径为50-500亩。第二片中的变形部分的厚度厚度小于变形前厚度的50％。该方法还包括用第一膜，第二膜和第一受害膜层叠第三膜以形成膜堆叠，在压制膜堆的同时在第三膜中形成变形和未变形的部分，蚀刻第三膜中的变形部分通过化学溶解所述部分，将具有孔的可变形的第四塑料膜和第二受害膜堆叠在复合膜上，用压力加压第二受害膜以将复合膜中的第四膜压制，使得形成变形部分以空腔和未变形区域的形式保留在第二受害者薄膜和第四薄膜中，并且去除第二受害薄膜。第三膜布置在第一膜和第二膜之间。第一，第二和第三部电影面临着第一部受害者电影。第三膜和第二膜相互连接以形成复合膜。

5. 发明授权

EP2650256B1 Verfahren zur Herstellung eines mikrostrukturierten Formkörpers 有权
标题翻译：一种用于制造微结构化模制过程
公开(公告)号：EP2650256B1
公开(公告)日：2015-12-09
申请号：EP13162904.0
申请日：2013-04-09
申请人： Technische Universität Ilmenau
发明人： Schober, Andreas , Hampl, Jörg , Weise, Frank , Schlingloff, Gregor , Fernekorn, Uta
IPC分类号： B81C1/00 , B81C99/00
CPC分类号： B44C1/227 , B81B2201/06 , B81B2203/0346 , B81B2207/056 , B81C99/0085 , B81C2201/034 , Y10T428/24281

6. 发明公开

EP2233564A3 Cell culture sheet comprising a functional substrate with a group of columnar micro-pillars and its manufacturing method 审中-公开
标题翻译：细胞培养载体，包括具有柱状微柱和制造方法的官能团的衬底
公开(公告)号：EP2233564A3
公开(公告)日：2012-11-21
申请号：EP10007188.5
申请日：2003-10-30
申请人： Hitachi, Ltd.
发明人： Miyauchi, Akihiro , Kuwabara, Kosuke , Ogino, Masahiko , Yoshida, Hiroshi , Ando, Takashi
IPC分类号： C12N5/00 , B01L3/00 , B81B1/00
CPC分类号： G02B1/111 , A45B25/18 , B01L3/502746 , B01L3/502761 , B01L2200/10 , B01L2200/12 , B01L2300/0636 , B01L2300/0877 , B01L2300/166 , B01L2400/086 , B81B1/00 , B81B2203/0361 , B81B2207/056 , B82Y20/00 , C12M25/00 , C12M25/06 , G01N27/423 , G02B1/005 , G02B1/118 , G02B5/289 , G02B6/12004 , G02B6/1225 , G02B6/42 , G02B6/43 , G02B2006/12147 , G02B2006/12166
摘要： The invention relates to a cell culture sheet (600) comprising a functional substrate with a group of columnar micro-pillars (604) extending from a matrix of an organic polymer.
The micro-pillars (604) have an equivalent diameter D in the range of 10 nm to 500 µm, a height H in the range of 50 nm to 5000 µm and an aspect ratio H/D of 4 or more. The cell culture sheet (600) may comprise gaps (605) for the flow of culture solution. The cell culture sheet (600) reduces damages of cultured cells.
The invention further relates to a method for manufacturing the cell culture sheets and a method for culturing cells and tissues.

7. 发明公开

EP2516322A2 VERFAHREN ZUR HERSTELLUNG VON KONUSFÖRMIGEN NANOSTRUKTUREN AUF SUBSTRATOBERFLÄCHEN 有权
标题翻译：用于生产锥形性纳米结构在衬底表面上
公开(公告)号：EP2516322A2
公开(公告)日：2012-10-31
申请号：EP10798264.7
申请日：2010-12-17
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
发明人： MORHARD, Christoph , PACHOLSKI, Claudia , SPATZ, Joachim, P.
IPC分类号： B81C1/00
CPC分类号： B81C1/00031 , B81B2203/0361 , B81B2207/056 , Y10T428/24479
摘要： The invention relates to a method for producing conical nanostructures on substrate surfaces, in particular in optical devices, solar cells, and sensors. The method according to the invention comprises at least the steps of: a) providing a substrate surface covered with nanoparticles; b) etching the substrate surface covered with nanoparticles, wherein the nanoparticles act as an etching mask and the etching parameters are set in such a way that hyperboloid structures are produced underneath the nanoparticles; c) breaking the hyperboloid structures in the region of the smallest diameter by exerting mechanical forces, wherein the structures remaining on the substrate surface have a conical shape.

8. 发明公开

EP2307307A2 MICRO-POSTS HAVING IMPROVED UNIFORMITY AND A METHOD OF MANUFACTURE THEREOF 审中-公开
标题翻译：具有产生改进的均匀性和过程的微型柱
公开(公告)号：EP2307307A2
公开(公告)日：2011-04-13
申请号：EP09773898.3
申请日：2009-06-30
申请人： Alcatel-Lucent USA Inc.
发明人： BOLLE, Cristian, A. , PARDO, Flavio
IPC分类号： B81B7/04 , H01L21/027
CPC分类号： H01L21/30604 , B81B2201/047 , B81B2203/0118 , B81B2203/0307 , B81B2203/0361 , B81B2203/0384 , B81B2207/056 , B81C1/00015 , B81C1/00039 , B81C1/00531 , B81C1/00539 , H01L21/3065 , Y10T428/24479
摘要： As discussed herein, there is presented an apparatus comprising micro-posts. The apparatus includes a substrate having a planar surface, a plurality of micro- posts located on the planar surface, wherein each micro- post has a base portion on the planar surface and a post portion located on a top surface of the corresponding base portion, and wherein side surfaces of the base portions intersect the planar surface at oblique angles.

9. 发明公开

EP2233564A2 Cell culture sheet comprising a functional substrate with a group of columnar micro-pillars and its manufacturing method 审中-公开
标题翻译：细胞培养载体，包括具有柱状微柱和制造方法的官能团的衬底
公开(公告)号：EP2233564A2
公开(公告)日：2010-09-29
申请号：EP10007188.5
申请日：2003-10-30
申请人： Hitachi, Ltd.
发明人： Miyauchi, Akihiro , Kuwabara, Kosuke , Ogino, Masahiko , Yoshida, Hiroshi , Ando, Takashi
IPC分类号： C12N5/00 , B01L3/00 , B81B1/00
CPC分类号： G02B1/111 , A45B25/18 , B01L3/502746 , B01L3/502761 , B01L2200/10 , B01L2200/12 , B01L2300/0636 , B01L2300/0877 , B01L2300/166 , B01L2400/086 , B81B1/00 , B81B2203/0361 , B81B2207/056 , B82Y20/00 , C12M25/00 , C12M25/06 , G01N27/423 , G02B1/005 , G02B1/118 , G02B5/289 , G02B6/12004 , G02B6/1225 , G02B6/42 , G02B6/43 , G02B2006/12147 , G02B2006/12166
摘要： The invention relates to a cell culture sheet (600) comprising a functional substrate with a group of columnar micro-pillars (604) extending from a matrix of an organic polymer.
The micro-pillars (604) have an equivalent diameter D in the range of 10 nm to 500 µm, a height H in the range of 50 nm to 5000 µm and an aspect ratio H/D of 4 or more. The cell culture sheet (600) may comprise gaps (605) for the flow of culture solution. The cell culture sheet (600) reduces damages of cultured cells.
The invention further relates to a method for manufacturing the cell culture sheets and a method for culturing cells and tissues.
摘要翻译：本发明涉及包含功能性基板与一组柱状微柱（604）由有机聚合物的基体延伸的一个细胞培养片（600）。的微柱（604）具有与等效直径D在10纳米至500微米，在50nm的范围内的高度H至5000微米，并且纵横比H / 4以上D中的范围内。细胞培养片（600）可以包括用于培养液的流动间隙（605）。细胞培养片材（600）降低培养的细胞的损伤。本发明还涉及一种用于制造细胞培养板和培养的细胞和组织的方法等。

10. 发明公开

EP2657004A4 METHOD FOR MANUFACTURING MICROSCOPIC STRUCTURAL BODY 有权
标题翻译： VERFAHREN ZUR HERSTELLUNG MIKROSKOPISCHERSTRUKTURKÖRPER
公开(公告)号：EP2657004A4
公开(公告)日：2017-04-12
申请号：EP11850115
申请日：2011-12-01
申请人： JAPAN STEEL WORKS LTD
发明人： NAITO AKIHIRO , FURUKI KENICHI , ITO HIROSHI , YAKEMOTO KAZUTOSHI
IPC分类号： B29C59/02 , B29C41/36 , B29C43/02 , B29C43/34 , B29C43/52 , H01L21/027
CPC分类号： B29C43/02 , B29C33/3857 , B29C33/424 , B29C39/24 , B29C39/26 , B29C39/42 , B29C41/36 , B29C41/50 , B29C43/021 , B29C43/34 , B29C43/52 , B29C45/263 , B29C2043/025 , B29C2043/3438 , B29C2043/5816 , B29C2059/023 , B81B2207/056 , B81C99/0085
摘要： The present invention is widely applicable to an injection molding method, an injection compression molding method, a blow molding method, a film sheet molding method using a roller and a thermoplastic molten polymer, and the like. An object of the invention is to provide a method for manufacturing a molded product with fine structure, which can transfer-mold a fine structure using any of various thermoplastic molten polymers without particularly requiring a pressurizing apparatus and can afford a nano-scaled fine pattern having a high economical efficiency, a large area, and homogeneous properties. The invention includes steps of, in a temperature-controlled stamper mold provided with a fine structure including a concavo-convex pattern having a width of 10 nm to 1 µm, forming a thermoplastic molten polymer layer to be in contact with the fine structure of the stamper mold having been kept at a predetermined temperature and holding the thermoplastic molten polymer layer for a predetermined time so as to transfer the fine structure of the stamper mold to the thermoplastic molten polymer layer under gravity.
摘要翻译：本发明可广泛地应用于注射成型法，注射压缩成型法，吹塑法，使用辊和热塑性熔融聚合物的膜片成型法等。本发明的目的是提供一种制造具有精细结构的模制产品的方法，其可以使用各种热塑性熔融聚合物中的任何一种转移模制精细结构，而不需要加压装置，并且可以提供纳米级精细图案，其具有经济效益高，面积大，性能均匀。本发明包括以下步骤：在具有包括宽度为10nm至1μm的凹凸图案的精细结构的温度控制的压模中，形成热塑性熔融聚合物层以与将模具保持在预定温度并将热塑性熔融聚合物层保持预定时间，以便在重力下将压模的精细结构转移到热塑性熔融聚合物层。

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