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    • 10. 发明公开
    • METHOD
    • 方法
    • EP2879513A2
    • 2015-06-10
    • EP13744574.8
    • 2013-08-02
    • Dupont Nutrition Biosciences ApS
    • ARENT, Susan LundLAURSEN, Brian SøgaardKIARIE, Elijah GituanjahMILLÁN, Luis Fernando RomeroZHANG, ZhenghongLAU, RosalynYU, ZheyongPEDERSEN, Mads BrøggerDALSGAARD, Søren
    • A23K1/06A23K1/14A23K1/165C12N9/24C12C5/00
    • A23K1/1656A23K10/14A23K10/38A23K20/189A23L7/115A23L29/06A23L33/21C12C5/004C12N9/2482Y02P60/873
    • The present invention relates to a method of preparing a corn based product said method comprising contacting a plant composition comprising (consisting of or consisting essentially of) corn or a corn by-product or a combination thereof with a xylanase comprising: i) a polypeptide as set forth in SEQ ID No. 8 or SEQ ID No. 7 or SEQ ID No. 6; or ii) a variant, fragment, homologue, fragment or derivative thereof having at least 85% identity with SEQ ID No. 8 or SEQ ID No. 7 or SEQ ID No. 6; or with a xylanase encoded by: a) a nucleotide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1; or b) a nucleotide sequence which can hybridize to the complement of SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1 under high stringency conditions: or c) a nucleotide sequence which has at least 80% identity with SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1. The present invention further relates to the use of the enzyme to produce corn based feedstuffs. The present invention yet further relates to a method of producing a fermented beverage comprising the step of contacting a mash and/or a wort with a xylanase comprising a polypeptide as set forth in SEQ ID No. 8 or SEQ ID No. 7 or SEQ ID No. 6; or a variant, fragment, homologue or derivative thereof having at least 85% identity with SEQ ID No. 8 or SEQ ID No. 7 or SEQ ID No. 6; or encoded by a nucleotide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1, or a nucleotide sequence which can hybridize to the complement of SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1 under high stringency conditions, or a nucleotide sequence which has at least 80% identity with SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1.
    • 本发明涉及一种制备玉米基产品的方法,所述方法包括使包含(由玉米或玉米副产品或其组合)(由玉米或玉米副产品或其组合)组成的植物组合物与包含以下成分的木聚糖酶接触:i) 如SEQ ID No.8或SEQ ID No.7或SEQ ID No.6所示; 或ii)与SEQ ID No.8或SEQ ID No.7或SEQ ID No.6具有至少85%同一性的变体,片段,同系物,片段或衍生物; 或由以下序列编码的木聚糖酶:a)本文中显示为SEQ ID No.3,SEQ ID No.2或SEQ ID No.1的核苷酸序列; 或b)在高度严格条件下可与SEQ ID No.3,SEQ ID No.2或SEQ ID No.1的互补序列杂交的核苷酸序列;或c)与SEQ ID No.3,SEQ ID No.2或SEQ ID No.1具有至少80%同一性的核苷酸序列 ID No.3,SEQ ID No.2或SEQ ID No.1。本发明进一步涉及该酶用于生产基于玉米的饲料的用途。 本发明还涉及生产发酵饮料的方法,包括使ma液和/或麦芽汁与包含如SEQ ID No.8或SEQ ID No.7或SEQ ID No.11所示的多肽的木聚糖酶接触的步骤 6号; 或与SEQ ID No.8或SEQ ID No.7或SEQ ID No.6具有至少85%同一性的变体,片段,同源物或衍生物; 或由本文中显示为SEQ ID No.3,SEQ ID No.2或SEQ ID No.1的核苷酸序列编码的核苷酸序列或能够与SEQ ID No.3,SEQ ID No.2的补体或 在高严格条件下的SEQ ID No.1,或与SEQ ID No.3,SEQ ID No.2或SEQ ID No.1具有至少80%同一性的核苷酸序列。