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1. 发明授权

EP1992689B1 METHOD FOR EXTRACTION OF NUCLEIC ACID FROM BIOLOGICAL MATERIAL 有权
标题翻译：过程对于核酸从生物学材料的提取
公开(公告)号：EP1992689B1
公开(公告)日：2015-03-18
申请号：EP07714626.4
申请日：2007-02-14
申请人： Tosoh Corporation
发明人： TSUCHIYA, Shigeo , HAYASHI, Toshinori
IPC分类号： C12N15/00 , C12N15/09
CPC分类号： C12N15/1006

2. 发明授权

EP2351851B1 METHOD FOR MEASURING CYTOKERATIN-19 MRNA 失效
标题翻译：方法细胞角蛋白19 mRNA的测量
公开(公告)号：EP2351851B1
公开(公告)日：2015-07-08
申请号：EP09829212.1
申请日：2009-11-30
申请人： Tosoh Corporation
发明人： OMOTO, Daisuke , SAITO, Juichi , OONAKA, Satoru , HAYASHI, Toshinori
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/6853 , C12Q1/6865 , C12Q2525/143 , C12Q2545/114 , C12Q2561/113 , C12Q2531/143 , C12Q2521/107

3. 发明公开

EP1783232A1 METHOD OF MEASURING HETEROGENOUS NCULEAR NIBONUCLEOPROTEIN B1 (hnRNP B1) mRNA 审中-公开
标题翻译：测量异源NCULEARNIBONUCERROTEIN B1（hnRNP B1）mRNA的方法
公开(公告)号：EP1783232A1
公开(公告)日：2007-05-09
申请号：EP05768811.1
申请日：2005-07-28
申请人： TOSOH CORPORATION
发明人： SAITO, Juichi , HAYASHI, Toshinori
IPC分类号： C12Q1/68 , C12N15/09 , G01N21/78
CPC分类号： G01N21/6428 , C12Q1/6865 , C12Q2563/107
摘要： A method for assaying heterogeneous nuclear ribonucleoprotein B1 (hnRNP B1) mRNA present in a sample, the method comprising a step of using a first primer homologous to at least a portion downstream from the 5'-end of a specified nucleotide sequence of the RNA and a second primer complementary to at least a portion upstream from the 3'-end of the specified nucleotide sequence to produce double-stranded DNA containing the promoter sequence and the specified nucleotide sequence downstream from the promoter sequence, wherein at least one of the first and second primers has a promoter sequence at the 5'-end, a step of using the double-stranded DNA as template to produce an RNA transcript, a step of using the RNA transcript in turn as template for DNA synthesis to produce the double-stranded DNA, a step of nucleic acid amplification in which the aforementioned steps are repeated under conditions that simultaneously promote each of the steps, and a step of assaying the amount of the RNA transcript.
摘要翻译：一种用于测定样品中存在的异源核核糖核蛋白B1（hnRNP B1）mRNA的方法，所述方法包括使用与所述RNA的特定核苷酸序列的5'端下游的至少一部分同源的第一引物和与指定核苷酸序列的3'末端上游的至少一部分互补的第二引物，以产生含有启动子序列和启动子序列下游的特定核苷酸序列的双链DNA，其中第一和第二引物中的至少一个第二引物在5'末端具有启动子序列，使用双链DNA作为模板以产生RNA转录本的步骤，使用RNA转录本依次作为DNA合成模板以产生双链 DNA，在同时促进各步骤的条件下重复上述步骤的核酸扩增步骤，以及分析RNA转录量的步骤脚本。

4. 发明公开

EP2202305A1 PRIMER FOR AMPLIFICATION OF RRNA OF BACTERIUM BELONGING TO THE GENUS LEGIONELLA, DETECTION METHOD, AND DETECTION KIT 审中-公开
标题翻译：引物军团菌属，检测方法和检测试剂盒细菌的R-RNA扩增
公开(公告)号：EP2202305A1
公开(公告)日：2010-06-30
申请号：EP08835360.2
申请日：2008-10-01
申请人： Tosoh Corporation , Intec Systems Institute, Incorporated
发明人： UNE, Kurando , SAITO, Juichi , HAYASHI, Toshinori , TAKIZAWA, Fumihide , HASHIMOTO, Yoichi
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/689 , Y02A50/451
摘要： [PROBLEMS] To detect a bacterium belonging to the genus Legionella rapidly, with high sensitivity, and specifically. [MEANS FOR SOLVING PROBLEMS] Disclosed are: a primer set for amplifying ribosomal RNA of a bacterium belonging to the genus Legionella specifically and highly efficiently; and a method for detecting ribosomal RNA of a bacterium belonging to the genus Legionella by using the primer set.
摘要翻译： [问题]为了检测细菌属于军团菌属快速，高灵敏度，并且具体。 [解决问题的手段]本发明公开了：用于扩增属于军团菌属特异性和高效的细菌的核糖体RNA的引物组; 和用于通过使用所述引物组检测属于军团菌属细菌的核糖体RNA的方法。

5. 发明公开

EP1790719A1 METHOD OF ASSAYING ALPHA 1,4-n-ACETYLGLUCOSAMINE TRANSFERASE (ALPHA 4GnT) mNRA 审中-公开
标题翻译：测定α1,4-n-乙酰葡萄糖胺转移酶（α4GnT）mNRA的方法
公开(公告)号：EP1790719A1
公开(公告)日：2007-05-30
申请号：EP05770350.6
申请日：2005-08-09
申请人： Tosoh Corporation
发明人： SAITO, Juichi , HAYASHI, Toshinori
IPC分类号： C12N15/09 , C12Q1/68 , G01N21/78
CPC分类号： C12Q1/6865 , C12Q2545/114
摘要： A method for assaying α1,4-N-acetylglucosamine transferase (α4GnT) mRNA present in a sample, the method comprising a step of using a first primer homologous to at least a portion downstream from the 5'-end of a specified nucleotide sequence of the RNA and a second primer complementary to at least a portion upstream from the 3'-end of the specified nucleotide sequence to produce double-stranded DNA containing the promoter sequence and the specified nucleotide sequence downstream from the promoter sequence, wherein at least one of the first and second primers has a promoter sequence at the 5'-end, a step of using the double-stranded DNA as template to produce an RNA transcript, a step of using the RNA transcript in turn as template for DNA synthesis to produce the double-stranded DNA, a step of nucleic acid amplification in which the aforementioned steps are repeated under conditions that simultaneously promote each of the steps, and a step of assaying the amount of the RNA transcript.
摘要翻译：一种分析样品中存在的α1,4-N-乙酰葡糖胺转移酶（α4GnT）mRNA的方法，该方法包括使用第一引物和第二引物的步骤，所述第一引物与下述特定核苷酸序列的5'末端下游的至少一部分同源所述RNA和与所述特定核苷酸序列的3'末端上游的至少一部分互补的第二引物，以产生含有所述启动子序列和所述启动子序列下游的特定核苷酸序列的双链DNA，其中至少一个第一和第二引物在5'末端具有启动子序列，使用双链DNA作为模板以产生RNA转录物的步骤，使用RNA转录物依次作为DNA合成模板以产生双链DNA，在同时促进每个步骤的条件下重复上述步骤的核酸扩增步骤，以及分析RNA转录物的量的步骤。

6. 发明授权

EP2345741B1 METHOD FOR MEASURING SURVIVIN mRNA 失效
标题翻译：方法的存活蛋白mRNA的测定
公开(公告)号：EP2345741B1
公开(公告)日：2014-09-10
申请号：EP09819288.3
申请日：2009-10-06
申请人： Tosoh Corporation
发明人： OMOTO, Daisuke , SAITO, Juichi , OONAKA, Satoru , HAYASHI, Toshinori
IPC分类号： C12Q1/68 , C12N15/09 , C12Q1/44 , C12Q1/48 , G01N33/53
CPC分类号： C12Q1/6886 , C12Q1/6851 , C12Q1/6865 , C12Q2600/158 , C12Q2525/143 , C12Q2563/173

7. 发明公开

EP2345741A1 METHOD FOR MEASURING SURVIVIN mRNA 失效
标题翻译： VERFAHREN ZUR SURVIVIN-MRNA-MESSUNG
公开(公告)号：EP2345741A1
公开(公告)日：2011-07-20
申请号：EP09819288.3
申请日：2009-10-06
申请人： Tosoh Corporation
发明人： OMOTO, Daisuke , SAITO, Juichi , OONAKA, Satoru , HAYASHI, Toshinori
IPC分类号： C12Q1/68 , C12N15/09 , C12Q1/44 , C12Q1/48 , G01N33/53
CPC分类号： C12Q1/6886 , C12Q1/6851 , C12Q1/6865 , C12Q2600/158 , C12Q2525/143 , C12Q2563/173
摘要： Disclosed is a method of amplifying and detecting mRNA of survivin gene in an RNA amplification process comprising: a step for forming a double-stranded DNA containing a promoter sequence by use of a combination of oligonucleotides consisting of a first primer having a sequence homologous with a portion of survivin mRNA and a second primer having a complementary sequence, wherein the promoter sequence is added to the 5'-end of either the first primer or the second primer with a reverse transcriptase, forming an RNA transcription product by use of an RNA polymerase with using the double-stranded DNA as template, and forming the double-stranded DNA by use of a reverse transcriptase by continuing to use the RNA transcription product as a template of DNA synthesis, measuring an amount of amplified RNA produced over time with an oligonucleotide probe designed so that signal properties change with the formation of a complementary double strand with the amplified RNA.
摘要翻译：公开了在RNA扩增方法中扩增和检测存活蛋白基因的mRNA的方法，包括：通过使用由具有与第一引物序列同源的第一引物组成的寡核苷酸组合形成含有启动子序列的双链DNA的步骤存在蛋白mRNA的部分和具有互补序列的第二引物，其中所述启动子序列用逆转录酶加入到第一引物或第二引物的5'-末端，通过使用RNA聚合酶形成RNA转录产物使用双链DNA作为模板，并通过继续使用RNA转录产物作为DNA合成的模板，通过使用逆转录酶形成双链DNA，测量随着时间的推移用寡核苷酸产生的扩增的RNA的量探针的设计使得信号特性随着扩增的RNA形成互补双链而发生变化。

8. 发明公开

EP0737858A1 METHOD AND APPARATUS FOR ADJUSTING ELECTRON-BEAM DEVICE 失效
标题翻译： VERFAHREN UNDGERÄTZUR JUSTIERUNG EINER ELEKTRONENSTRAHLVORRICHTUNG
公开(公告)号：EP0737858A1
公开(公告)日：1996-10-16
申请号：EP94918545.8
申请日：1994-06-22
申请人： RESEARCH DEVELOPMENT CORPORATION OF JAPAN , TOSOH CORPORATION
发明人： TSUKAJIMA, Junichi , HAYASHI, Toshinori , ENOKIJIMA, Toru
IPC分类号： G01N23/225
CPC分类号： H01J37/256 , H01J2237/2522
摘要： In an electron beam apparatus having an energy analyzer for analyzing the electron beam energy, matching may be performed accurately and easily of the position to be irradiated by an electron beam and the position for which an energy analysis is performed. The electron beam apparatus includes: an electron-optical lens column 401 provided as capable of scanning an electron beam ED in an XY direction and capable of deflecting it in the XY direction; an energy analyzer 408; and image display means 416 for displaying an SEM image of a predetermined range on a sample 407 by an electron beam scanning. An energy analysis area 504 for which electrons are efficiently taken into the energy analyzer is displayed as an image upon the SEM image by analyzing an output information obtained at the energy analyzer 408 at the time of an electron beam scanning. An analyzing electron-beam irradiation position 502 to be irradiated by an electron beam on the trajectory emitted from the electron-optical lens column 401 at the time corresponding to the non-scanning state of the electron beam is displayed as an image upon the SEM image. The trajectory of the electron beam to be irradiated onto the sample is deflected so that the energy analysis area 504 and the analyzing electron-beam irradiation position 502 are matched on the SEM image.
摘要翻译：在具有用于分析电子束能量的能量分析器的电子束装置中，可以准确且容易地执行与电子束的照射位置和进行能量分析的位置的匹配。电子束装置包括：电子 - 光学透镜柱401，其能够沿XY方向扫描电子束ED并能够在XY方向上偏转; 能量分析器408; 以及用于通过电子束扫描在样本407上显示预定范围的SEM图像的图像显示装置416。通过分析在电子束扫描时在能量分析器408处获得的输出信息，将能量分析区域504有效地吸入能量分析器的能量分析区域504作为图像显示在SEM图像上。在与电子束的非扫描状态相对应的时刻，从电子光学透镜柱401发射的轨迹上的电子束照射的分析电子束照射位置502作为图像显示在SEM图像上。要照射到样品上的电子束的轨迹被偏转，使得能量分析区域504和分析电子束照射位置502在SEM图像上匹配。

9. 发明公开

EP2351851A1 METHOD FOR MEASURING CYTOKERATIN-19 MRNA 失效
标题翻译：测量细胞角蛋白-19MRNA的方法
公开(公告)号：EP2351851A1
公开(公告)日：2011-08-03
申请号：EP09829212.1
申请日：2009-11-30
申请人： Tosoh Corporation
发明人： OMOTO, Daisuke , SAITO, Juichi , OONAKA, Satoru , HAYASHI, Toshinori
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/6853 , C12Q1/6865 , C12Q2525/143 , C12Q2545/114 , C12Q2561/113 , C12Q2531/143 , C12Q2521/107
摘要： Disclosed is a method of amplifying and detecting cytokeratin 19 mRNA in RNA amplification process, comprising: a step for forming a double-stranded DNA containing a promoter sequence with a reverse transcriptase by use of a combination of oligonucleotides consisting of a first primer having a sequence homologous to a portion of cytokeratin 19 mRNA and a second primer having a complementary sequence, wherein the promoter sequence is added to the 5'-end of either the first primer or the second primer, forming an RNA transcription product by use of an RNA polymerase with using the double-stranded DNA as template, and forming the double-stranded DNA by use of a reverse transcriptase by continuing to use the RNA transcription product as a template of DNA synthesis, by measuring an amount of amplified RNA produced over time with an oligonucleotide probe designed so that signal properties change with the formation of a complementary double strand with the amplified RNA.
摘要翻译：本发明公开了在RNA扩增过程中扩增和检测细胞角蛋白19mRNA的方法，其包括：用逆转录酶形成含有启动子序列的双链DNA的步骤，其中使用由具有序列的第一引物与细胞角蛋白19 mRNA的一部分同源，以及具有互补序列的第二引物，其中启动子序列被添加到第一引物或第二引物的5'末端，通过使用RNA聚合酶形成RNA转录产物以双链DNA为模板，通过继续使用RNA转录产物作为DNA合成的模板，通过使用逆转录酶形成双链DNA，通过测量随时间产生的扩增RNA的量寡核苷酸探针，其设计使得信号特性随着与扩增的RNA形成互补双链而改变。

10. 发明公开

EP2172561A1 IMPROVED METHOD OF DETECTING NOROVIRUS RNA 审中-公开
标题翻译： VERBESSERTES VERFAHRENFÜRDEN NACHWEIS VON NOROVIRUS-RNA
公开(公告)号：EP2172561A1
公开(公告)日：2010-04-07
申请号：EP08777360.2
申请日：2008-06-12
申请人： Tosoh Corporation
发明人： MASUDA, Noriyoshi , UNE, Kurando , SAITO, Juichi , HAYASHI, Toshinori
IPC分类号： C12Q1/68 , C12N15/09 , G01N21/78 , G01N33/569
CPC分类号： C12Q1/701 , C12Q2563/173 , C12Q2531/143 , C12Q2521/107
摘要： The amount of an RNA transcription product amplified in an RNA amplification process is measured using a nucleic acid probe labeled with an intercalating fluorescent dye. The RNA amplification process comprises the steps of using at least two sets of primer pairs comprising a first primer and a second primer (in which one of these primers carries a promoter sequence added to the 5' end thereof), both of which have high hybridization efficiency to a nucleic acid sequence that is homologous to or complementary to each norovirus genotype RNA; forming a double-stranded DNA containing the promoter sequence with a reverse transcriptase; forming an RNA transcription product with an RNA polymerase by using the double-stranded DNA as a template; and forming the double-stranded DNA by successively using the RNA transcription product as a template in the DNA synthesis with the reverse transcriptase.
摘要翻译：使用用插层荧光染料标记的核酸探针测量在RNA扩增过程中扩增的RNA转录产物的量。 RNA扩增方法包括以下步骤：使用至少两组包含第一引物和第二引物的引物对（其中这些引物中的一个携带加入其5'末端的启动子序列），两者都具有高杂交与每种诺如病毒基因型RNA同源或互补的核酸序列的效率; 用逆转录酶形成含有启动子序列的双链DNA; 通过使用双链DNA作为模板与RNA聚合酶形成RNA转录产物; 并通过在逆转录酶的DNA合成中连续使用RNA转录产物作为模板形成双链DNA。

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