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2. 发明公开

EP2546341A1 FC BINDING PROTEIN AND METHOD FOR PRODUCING SAME 有权
标题翻译： FC结合蛋白及其生产方法
公开(公告)号：EP2546341A1
公开(公告)日：2013-01-16
申请号：EP11753056.8
申请日：2011-03-10
申请人： Sagami Chemical Research Institute , Tosoh Corporation
发明人： HATAYAMA, Kouta , ASAOKA, Yoshiharu , TANAKA, Toru , IDE, Teruhiko
IPC分类号： C12N15/09 , C07K14/735 , C12N1/21 , C12P21/02
CPC分类号： C07K14/70535 , C07K1/22 , C07K17/08 , C07K2319/21
摘要： Disclosed are: an Fc binding protein having increased stability with respect to heat, acid, and/or alkalinity compared with the wild type; a method for producing same; and a method for specifically isolating protein containing an Fc binding protein binding site using said Fc binding protein as a ligand for affinity chromatography.
An Fc binding protein was obtained having increased stability with respect to heat, acid, and/or alkalinity compared with the wild-type human Fc receptor by means of substituting at least one specific amino acid residue in the extracellular domain of the wild-type human Fc receptor with another amino acid residue. The Fc binding protein is useful as a ligand for affinity chromatography for example by immobilizing in a solid phase. Also, when the Fc binding protein is expressed using a host that has been transformed with an expression vector containing a polynucleotide coding for said protein, the amount of produced protein is increased compared with using a wild-type human Fc receptor.
摘要翻译：公开了：与野生型相比，具有增加的热，酸和/或碱度稳定性的Fc结合蛋白; 其制造方法; 以及使用所述Fc结合蛋白作为亲和层析的配体特异性分离含有Fc结合蛋白结合位点的蛋白的方法。通过取代野生型人类细胞外结构域中的至少一个特定氨基酸残基，获得与野生型人类Fc受体相比具有关于热，酸和/或碱度增加的稳定性的Fc结合蛋白 Fc受体与另一个氨基酸残基。 Fc结合蛋白可用作亲和层析的配体，例如通过固定在固相中。此外，当使用已经用含有编码所述蛋白质的多核苷酸的表达载体转化的宿主表达Fc结合蛋白时，与使用野生型人Fc受体相比，产生的蛋白质的量增加。

3. 发明公开

EP3048112A1 Fc-BINDING PROTEIN, METHOD FOR PRODUCING SAID PROTEIN, AND ANTIBODY ADSORBENT USING SAID PROTEIN, AND METHODS FOR PURIFYING AND IDENTIFYING ANTIBODY USING SAID ADSORBENT 有权
标题翻译： FC-结合蛋白，过程以对其及其制备ANTIKÖRPERADSORPTIONSMITTEL与清洁和抗体识别这种蛋白质及其使用方法该吸附剂
公开(公告)号：EP3048112A1
公开(公告)日：2016-07-27
申请号：EP14846295.5
申请日：2014-09-18
申请人： Tosoh Corporation
发明人： ASAOKA, Yoshiharu , TANAKA, Toru , IDE, Teruhiko
IPC分类号： C07K14/735 , C07K1/22 , C12N1/21 , C12N15/09 , C12P21/02
CPC分类号： C07K14/70535 , C07H1/06 , C07K1/22 , C07K16/283 , C07K17/00 , C07K2317/10 , C07K2317/732
摘要： The present invention addresses the first problem of providing an Fc-binding protein having improved stability, especially stability to heat and acid, of the Fc-binding protein, a method for producing this protein, and an antibody adsorbent using this protein. The present invention also addresses the second problem of providing a method that makes it possible to identify the presence or absence of glycosylation of an antibody, and a material to be used in this method. The first problem is solved by an Fc-binding protein having improved stability to heat and acid obtained by substituting amino acid residues at specific positions in the extracellular domain within human FcγRIIIa with other specific amino acids, a method for producing this protein, and an antibody adsorbent using this protein. The second problem is solved by using an adsorbent capable of specifically adsorbing an antibody having a sugar chain, the adsorbent being obtained by immobilizing human FcγRIIIa on an insoluble carrier.
摘要翻译：本发明解决了具有改进的稳定性，尤其是爱对热的稳定性和酸，所述Fc结合蛋白，用于产生该蛋白的方法，以及使用该蛋白质与抗体吸附剂的第一个问题，提供的与Fc结合蛋白。因此，本发明针对的是第二提供一种方法问题的做使得有可能识别存在或不存在的抗体的糖基化的，并且材料在该方法中使用。第一个问题，是通过求解以Fc结合蛋白具有改善的稳定性，对热和酸通过与其他特定的氨基酸，用于制造这种蛋白的方法人类Fc³RIIIa内的细胞外结构域替换在特定位置的氨基酸残基得到的，到抗体吸附剂使用这种蛋白质。第二个问题，是通过使用能够特异性吸附到抗体，其具有糖链吸附剂的解决，所述吸附剂是由上到不溶性载体固定化人Fc³RIIIa得到。

4. 发明公开

EP4083059A1 IMPROVED ADENO-ASSOCIATED VIRUS-BINDING PROTEIN, METHOD FOR PRODUCING SAME, AND ADENO-ASSOCIATED VIRUS ADSORBENT USING SAME 审中-实审
公开(公告)号：EP4083059A1
公开(公告)日：2022-11-02
申请号：EP20893928.0
申请日：2020-11-24
申请人： Tosoh Corporation
发明人： ASAOKA, Yoshiharu , KOBAYASHI, Hidetaka , MAKINO, Yuriko , YOSHIDA, Kouhei , KURIHARA, Kento
IPC分类号： C07K14/705 , C12N7/00 , C12N1/21 , C12N15/12 , C12N15/63 , C12P21/00
摘要： The present invention provides an improved adeno-associated virus (AAV)-binding protein having enhanced stability, especially , heat stability, acid stability and alkali stability, of an AAV-binding protein, a method for producing the improved AAV-binding protein, and an AAV adsorbent using the same.

5. 发明授权

EP3048112B1 Fc-BINDING PROTEIN, METHOD FOR PRODUCING SAID PROTEIN, AND ANTIBODY ADSORBENT USING SAID PROTEIN, AND METHODS FOR PURIFYING AND IDENTIFYING ANTIBODY USING SAID ADSORBENT 有权
公开(公告)号：EP3048112B1
公开(公告)日：2020-03-11
申请号：EP14846295.5
申请日：2014-09-18
申请人： Tosoh Corporation
发明人： ASAOKA, Yoshiharu , TANAKA, Toru , IDE, Teruhiko
IPC分类号： C07K14/735 , C07K1/22 , C12N1/21 , C12N15/09 , C12P21/02 , C07H1/06

6. 发明授权

EP2546341B1 FC BINDING PROTEIN AND METHOD FOR PRODUCING SAME 有权
标题翻译： FC-结合蛋白和方法及其
公开(公告)号：EP2546341B1
公开(公告)日：2014-12-31
申请号：EP11753056.8
申请日：2011-03-10
申请人： Sagami Chemical Research Institute , Tosoh Corporation
发明人： HATAYAMA, Kouta , ASAOKA, Yoshiharu , TANAKA, Toru , IDE, Teruhiko
IPC分类号： C12N15/09 , C07K14/735 , C12N1/21 , C12P21/02
CPC分类号： C07K14/70535 , C07K1/22 , C07K17/08 , C07K2319/21

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