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1. 发明公开

EP3164696A1 VERFAHREN UND VORRICHTUNG ZUR RAMANSPEKTROSKOPISCHEN IN-OVO GESCHLECHTSBESTIMMUNG VON BEFRUCHTETEN UND BEBRÜTETEN VOGELEIERN 有权
标题翻译：方法和设备IN-拉曼光谱OVO性别鉴定和受精孵化鸟蛋
公开(公告)号：EP3164696A1
公开(公告)日：2017-05-10
申请号：EP15759358.3
申请日：2015-07-03
申请人： Technische Universität Dresden , Universität Leipzig
发明人： GALLI, Roberta , PREUSSE, Grit , KOCH, Edmund , STEINER, Gerald , KRAUTWALD-JUNGHANNS, Maria-Elisabeth , BARTELS, Thomas
IPC分类号： G01N21/65 , G01N33/08
CPC分类号： G01N21/65 , A01K45/007 , G01N33/08 , G01N33/4833 , G01N2021/653 , G01N2201/06113
摘要： The invention relates to a method for the Raman spectroscopic, in ovo sex determination of fertilised and hatched birds' eggs (1), wherein the embryo, including the extra-embryonic structures, can move in the egg, and is not yet attached to the shell at the time of measuring. In addition, the following steps are carried out: monitoring the time course of the hatched egg until forming at least one recognisable blood vessel (21); creating a hole (2) in the shell in the region near to the attached bloody vessel, using a hole-generating unit; finding the blood vessel forming in the egg, using a vision system (19, 13) and a coaxial or lateral illumination with light (10a) in the visible wavelength range; positioning at least one blood vessel in the laser focus of a laser source (3), either by moving the egg or moving a lens (6) of a device (5) for introducing the laser light (3a), and detecting the Raman scattered radiation (7); registering the Raman scattered radiation of the irradiated blood vessel using the device for introducing the laser light, and for detecting the Raman scattered radiation, wherein, during the measuring process, a movement of the blood vessel out of the focus can be avoided by tracking using the vision system; evaluating the Raman scattered radiation in an evaluation unit; determining and displaying the sex of the embryo in the bird's egg.
摘要翻译：本发明涉及一种拉曼光谱的方法，在卵内性别决定受精和孵化的鸟蛋（1）的，worin胚胎，包括胚外的结构，可在蛋移动，并且还没有被附连到壳在测量的时间。此外，下面的步骤被执行：监测蛋孵出的时间过程，直至形成至少一个可识别的血管（21）; 创建在该区域壳中的孔（2）接近附血性容器，使用空穴产生单元; 找到血管在卵形成，使用视觉系统（19，13）和在可见光波长范围内的光（10A）同轴或侧向照明; 定位至少一个血管中的激光源（3）任一的激光焦点通过移动卵或移动透镜（6）用于将激光（3a）中，并检测拉曼的装置（5）的散辐射（7）; 注册拉曼使用该装置用于将激光光散射所照射的血管的辐射，并且用于检测拉曼散射辐射，worin，在测量过程中，可以通过使用跟踪来避免使血管的移动出来的焦点的视觉系统; 评价拉曼至评估单元的散射辐射; 确定性的挖掘和展示在鸟的蛋胚胎的性别。

2. 发明公开

EP2526405A1 VERFAHREN UND VORRICHTUNG ZUR BESTIMMUNG DES GESCHLECHTES VON BEFRUCHTETEN UND NICHT BEBRÜTETEN VOGELEIERN 有权转让
标题翻译：的方法和设备，用于确定性别和受精的未孵育鸟蛋
公开(公告)号：EP2526405A1
公开(公告)日：2012-11-28
申请号：EP11711247.4
申请日：2011-01-14
申请人： Technische Universität Dresden , Universität Leipzig
发明人： STEINER, Gerald , KOCH, Edmund , KRAUTWALD-JUNGHANNS, Maria-Elisabeth , BARTELS, Thomas
IPC分类号： G01N21/35 , G01N21/55 , G01N33/74
CPC分类号： A01K45/007 , G01N21/3563 , G01N21/3577 , G01N21/359 , G01N21/552 , G01N33/08 , G01N33/743 , G01N2021/3595
摘要： The invention relates to a method and a device (1, 100) for determining the sex of fertilized, nonincubated bird eggs (13, 130), where an egg (13, 130) has a solid egg shell (14), an egg yolk (2) which is surrounded by the egg shell and further egg integuments and a blastodisk(3) associated with the egg yolk (2), and where a probe (4, 40) for measuring a spectrum is introduced through a hole (17) of the egg shell (14) towards the blastodisk (3) with blastodisk cells (23), with the following steps: - positioning of the probe (4, 40) in the region of the blastodisk (3), - spectroscopic
in-ovo characterization of the blastodisk cells (23), and - identification of the sex by an automatic classification of spectra. The problem is that of rapidly, reliably and unambiguously determining the sex as early as during the bird egg stage. The solution is as follows: an optical crystal is employed as the probe (4, 40), by means of which optical crystal a rapid and retroaction-free recording of an infrared and/or near-infrared spectrum (70) is carried out by exploiting a reduced total reflection (31) within the optical crystal (4, 40) through an evanescent field (21) within the region (29) of the blastodisk (3), with extinction taking place as a result of a spectral absorption of sex-specific blastodisk cells (23), where the positioning of the optical crystal (4, 40) is accompanied by an ongoing automatic evaluation of the returned spectra (70) until the sex-specific blastodisk cells (23) are determined, until the sex of the fertilized egg (13, 130) is evaluated by an evaluation unit (7) and unambiguously displayed by a display unit (12).

3. 发明授权

EP2526405B1 VERFAHREN UND VORRICHTUNG ZUR BESTIMMUNG DES GESCHLECHTES VON BEFRUCHTETEN UND NICHT BEBRÜTETEN VOGELEIERN 有权转让
标题翻译：的方法和设备，用于确定性别和受精的未孵育鸟蛋
公开(公告)号：EP2526405B1
公开(公告)日：2016-10-19
申请号：EP11711247.4
申请日：2011-01-14
申请人： Technische Universität Dresden , Universität Leipzig
发明人： STEINER, Gerald , KOCH, Edmund , KRAUTWALD-JUNGHANNS, Maria-Elisabeth , BARTELS, Thomas
IPC分类号： G01N21/35 , G01N21/55 , G01N33/74 , G01N33/08 , G01N21/3563 , A01K45/00 , G01N21/552 , G01N21/3577 , G01N21/359
CPC分类号： A01K45/007 , G01N21/3563 , G01N21/3577 , G01N21/359 , G01N21/552 , G01N33/08 , G01N33/743 , G01N2021/3595

4. 发明授权

EP3164696B1 VERFAHREN UND VORRICHTUNG ZUR RAMANSPEKTROSKOPISCHEN IN-OVO GESCHLECHTSBESTIMMUNG VON BEFRUCHTETEN UND BEBRÜTETEN VOGELEIERN 有权
标题翻译：方法和设备的RAMANSPEKTROSCOPIC INOOVO法律确定脆弱和破碎的鸟
公开(公告)号：EP3164696B1
公开(公告)日：2018-04-18
申请号：EP15759358.3
申请日：2015-07-03
申请人： Technische Universität Dresden , Universität Leipzig
发明人： GALLI, Roberta , PREUSSE, Grit , KOCH, Edmund , STEINER, Gerald , KRAUTWALD-JUNGHANNS, Maria-Elisabeth , BARTELS, Thomas
IPC分类号： G01N21/65 , G01N33/08 , G01N33/483
CPC分类号： G01N21/65 , A01K45/007 , G01N33/08 , G01N33/4833 , G01N2021/653 , G01N2201/06113

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