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1. 发明公开

EP0716150A1 DNA primer and a method for screening DNAS 失效
标题翻译： DNS-Primer und Screening-VerfahrenfürDNS
公开(公告)号：EP0716150A1
公开(公告)日：1996-06-12
申请号：EP95119121.2
申请日：1995-12-05
申请人： Takeda Chemical Industries, Ltd.
发明人： Onda, Haruo , Hosoya, Masaki
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04 , G01N33/68
CPC分类号： C12Q1/6876 , C07K5/0806 , C07K14/57536
摘要： DNA primers each having a nucleotide sequence substantially complementary to a nucleotide sequence which codes for Gly-Lys-Arg, Gly-Lys-Lys or Gly-Arg-Arg; methods for amplifying a polynucleotide which codes for a peptide or a precursor protein thereof using said DNA primer; screening methods for a polynucleotide which codes for a peptide comprising an amino acid sequence of Gly-Lys-Arg, Gly-Lys-Lys or Gly-Arg-Arg or a precursor protein thereof from a DNA library, etc. using said DNA; the polynucleotide obtained by said screening method; and the peptide or its precursor protein encoded by said polynucleotide, or a salt thereof. When the DNA primer of the present invention is used, the DNA which codes for a peptide having a useful physiological activity or a precursor protein thereof can be efficiently cloned.
摘要翻译： DNA引物各自具有与编码Gly-Lys-Arg，Gly-Lys-Lys或Gly-Arg-Arg的核苷酸序列基本上互补的核苷酸序列; 使用所述DNA引物扩增编码肽或其前体蛋白的多核苷酸的方法; 使用所述DNA从DNA文库等编码包含Gly-Lys-Arg，Gly-Lys-Lys或Gly-Arg-Arg或其前体蛋白质的氨基酸序列的肽的多核苷酸的筛选方法; 通过所述筛选方法获得的多核苷酸; 和由所述多核苷酸编码的肽或其前体蛋白质或其盐。当使用本发明的DNA引物时，可以有效地克隆编码具有有用生理活性的肽或其前体蛋白质的DNA。

2. 发明公开

EP0470604A3 PACAP38, proteine with adenylate cyclase activity 失效
标题翻译： PACAP38，具有腺苷酸环化酶活性的蛋白质
公开(公告)号：EP0470604A3
公开(公告)日：1992-12-09
申请号：EP91113293.4
申请日：1991-08-08
申请人： Takeda Chemical Industries, Ltd.
发明人： Onda, Haruo , Kimura, Chiharu
IPC分类号： C12N15/16 , C07K14/00 , C12N1/21 , C12P21/02
CPC分类号： C07K14/57563
摘要： Disclosed are (1) a genomic DNA of human PACAP38; (2) a DNA containing a DNA segment coding for human PACAP38; (3) a transformant bearing a DNA containing a DNA segment coding for PACAP38; and (4) a method for preparing a mature PACAP38 protein comprising cultivating the transformant described in the above (3), accumulating the protein in a culture product, and collecting the resulting protein. The DNA makes it possible to screen the chemical substance necessary for production of PACAP and is applied to experimental animals to understand their brain functions, which serves to elucidate human brain functions. Human PACAP38 can also be utilized as therapeutic agents about growth and maintenance of human brain nerves.

3. 发明公开

EP0385308A1 Production of a peptide from human cancer cells 失效
标题翻译： Herstellung eines Peptids aus menschlichen Krebszellen。
公开(公告)号：EP0385308A1
公开(公告)日：1990-09-05
申请号：EP90103566.7
申请日：1990-02-23
申请人： Takeda Chemical Industries, Ltd.
发明人： Onda, Haruo , Itoh, Yasuaki , Hayashi, Kyozo
IPC分类号： C12N15/12 , C07K7/00 , C12P21/02 , C12N1/18
CPC分类号： C07K14/47 , A61K38/00
摘要： Disclosed are (1) a DNA containing a DNA segment coding for a polypeptide having EAQ as the N-terminal sequence and composed of 60 amino acids, (2) a recombinant DNA constructed by introducing the DNA sequence in an expression vector for Escherichia coli , yeast or animal cells so as to express the foregoing polypeptide, (3) a transformant bearing the recombinant DNA and (4) a process for producing the foregoing polypeptide comprising cultivating the transformant, accumulating the polypeptide and collecting the same, whereby the polypeptide can be obtained in large amounts and is useful for the elucidation of the growth mechanism of cancer cells and the development of the therapeutic agents for carcinoma.
摘要翻译：公开了（1）含有编码具有EAQ作为N末端序列并由60个氨基酸组成的多肽的DNA区段的DNA，（2）通过将DNA序列引入大肠杆菌表达载体构建的重组DNA，酵母或动物细胞，以表达前述多肽，（3）携带重组DNA的转化体和（4）产生前述多肽的方法，包括培养转化体，聚集多肽并收集该多肽，由此多肽可以是大量获得，可用于阐明癌细胞的生长机制和癌症治疗剂的发展。

4. 发明公开

EP0279433A2 Plants resistant to cucumber mosaic virus infection 失效
标题翻译： Gegen“黄瓜花叶病毒” - 抗病毒Pflanzen。
公开(公告)号：EP0279433A2
公开(公告)日：1988-08-24
申请号：EP88102322.0
申请日：1988-02-18
申请人： Takeda Chemical Industries, Ltd.
发明人： Furusawa, Iwao , Onda, Haruo , Komiya, Takeya
IPC分类号： C12N15/40 , C12N15/82
CPC分类号： C07K14/005 , A01H1/02 , C12N15/8283 , C12N2770/14022
摘要： This invention presents a DNA coding for the coat protein of the cucumber mosaic virus strain Y, a vector DNA containing the DNA and a transformant transformed with said vector DNA, and it also relates to a production of genetically transformed plants which are resistant to cucumber mosaic virus infection.
摘要翻译：本发明提供了编码黄瓜花叶病毒株Y的外壳蛋白的DNA，含有DNA的载体DNA和用所述载体DNA转化的转化体，并且还涉及对黄瓜镶嵌抗性的转基因植物的生产病毒感染。

5. 发明公开

EP0068719A2 Deoxyribonucleic acids, their production and use 失效
标题翻译： DesoxyribonukleineSäuren，deren Herstellung und Verwendung。
公开(公告)号：EP0068719A2
公开(公告)日：1983-01-05
申请号：EP82303122.4
申请日：1982-06-16
申请人： Takeda Chemical Industries, Ltd.
发明人： Igarashi, Koichi , Onda, Haruo , Kikuchi, Masakazu , Fujisawa, Yukio
IPC分类号： C12N15/00 , C12P21/02 , C07H21/04 , C07C103/52 , C12N1/20 , A61K39/29
CPC分类号： C07K14/005 , A61K39/00 , C07K2319/00 , C07K2319/40 , C07K2319/61 , C12N15/70 , C12N2730/10122
摘要： A recombinant DNA containing a structural gene coding for hepatitis B virus subtype adw surface antigen is constructed. This structural gene is expressed in a transformant containing said recombinant DNA to produce hepatitis B virus subtype adw surface antigen which is useful as a vaccine against hepatitis B.
摘要翻译：构建了含有编码乙型肝炎病毒亚型adw表面抗原的结构基因的重组DNA。该结构基因在含有所述重组DNA的转化体中表达，以产生可用作针对乙型肝炎的疫苗的乙型肝炎病毒亚型adw表面抗原。

6. 发明公开

EP0442538A1 High purified protein, production and use thereof 失效
标题翻译： Hochgereinigtes蛋白，seine Herstellung und Verwendung。
公开(公告)号：EP0442538A1
公开(公告)日：1991-08-21
申请号：EP91105904.6
申请日：1984-11-23
申请人： Takeda Chemical Industries, Ltd.
发明人： Kato, Koichi , Yamada, Takao , Onda, Haruo
IPC分类号： C12N15/26 , C12P21/02 , A61K37/02 , C07K13/00
CPC分类号： C07K14/55 , A61K38/00
摘要： A substantially pure non-glycosylated human interleukin-2 protein having a specific activity of not less than 10⁴ U/mg is obtained by growing a transformant carrying a DNA having a base sequence coding for human interleukin-2 to cause production and accumulation of human interleukin-2 in the culture broth, and subjecting the thus obtained human interleukin-2-containing liquid to a purification process comprising a hydropholic column chromatography.
摘要翻译：通过生长携带具有编码人白细胞介素-2的碱基序列的DNA以产生和积累的转化体，获得具有不小于10 4 U / mg的比活性的基本上纯的非糖基化人白细胞介素-2蛋白质的培养肉汤中的人白细胞介素-2，并将由此获得的含白细胞介素-2的液体进行包含疏水柱色谱法的纯化方法。

7. 发明公开

EP0315118A2 DNA coding for endothelin and use thereof 失效
标题翻译：内皮素脱氧核糖核酸和dessen Verwendung。
公开(公告)号：EP0315118A2
公开(公告)日：1989-05-10
申请号：EP88118171.3
申请日：1988-11-01
申请人： Takeda Chemical Industries, Ltd.
发明人： Masaki, Tomoh , Yanagisawa, Masashi , Kurihara, Hiroki , Onda, Haruo , Itoh, Yasuaki , Fujino, Masahiko
IPC分类号： C12N15/00 , C07K7/10 , C12N1/20 , C12N1/18 , C12P21/02 , A61K37/02
CPC分类号： C07K14/57536 , A61K38/00
摘要： (1) a DNA sequence containing a DNA segment coding for porcine or human endothelin, (2) a precursor protein of porcine or human endothelin, (3) a transformant bearing a DNA sequence containing a DNA segment coding for percine or human endothelin, and (4) a method for preparing mature porcine or human endothelin or a porcine or human endothelin precursor are disclosed.
The cells gene-infected or transformed with the DNA of the present invention can allow a large amount of endothelin to be produced, even in various cells in which little or no endothelin is originally produced. Therefore, the production of endothelin can be advantageously achieved.
Endothelin can be utilized as hypotension therapeutic agents or local vasoconstrictors to animals including humans.
摘要翻译：（1）含有编码猪或人内皮素的DNA片段的DNA序列，（2）猪或人内皮素的前体蛋白质，（3）具有含有编码海马或人内皮素的DNA片段的DNA序列的转化体，以及（4）公开了制备成熟猪或人内皮素或猪或人内皮素前体的方法。用本发明的DNA感染或转化的细胞可以允许产生大量的内皮素，即使在最初产生很少或没有内皮素的各种细胞中也是如此。因此，可以有利地实现内皮素的产生。内皮素可用作包括人在内的动物的低血压治疗剂或局部血管收缩剂。

8. 发明公开

EP0293077A1 Assay for anti-pre-S antibody 失效
标题翻译：测定抗前S抗体
公开(公告)号：EP0293077A1
公开(公告)日：1988-11-30
申请号：EP88303467.0
申请日：1988-04-18
申请人： Takeda Chemical Industries, Ltd.
发明人： Fujisawa, Yukio , Onda, Haruo , Hinuma, Shuji
IPC分类号： G01N33/576 , G01N33/569
CPC分类号： G01N33/5764
摘要： An assay for anti-pre-S antibody in a serum specimen which comprises adding a carrier having Protein A bound thereto to a mixture containing the serum specimen and a pre-S peptide labeled with ¹²⁵I, then separating the carrier from the mixture, and measuring radioactivity of the separated carrier is highly specific, highly sensitive and simply operable.
摘要翻译：血清标本中抗前S抗体的测定法包括将含有结合有蛋白A的载体加入到含有血清标本和用125 I标记的前S肽的混合物中，然后从混合物中分离载体，并测量分离的载体的放射性高度特异，高度敏感并且可操作。

9. 发明授权

EP0102634B1 Recombinant DNA, microorganism transformed therewith and use thereof 失效
标题翻译：重组DNA，其转化的微生物及其用途
公开(公告)号：EP0102634B1
公开(公告)日：1988-06-29
申请号：EP83108699.6
申请日：1983-09-03
申请人： Takeda Chemical Industries, Ltd.
发明人： Kikuchi, Masakazu , Kurokawa, Tsutomu , Onda, Haruo
IPC分类号： C12N15/00 , C12P21/00 , C12P21/04 , C12P19/34 , A61K39/395
CPC分类号： C07K16/00 , C12N15/70

10. 发明公开

EP0158198A1 DNA and use thereof 失效
标题翻译： DNA及其用途
公开(公告)号：EP0158198A1
公开(公告)日：1985-10-16
申请号：EP85103450.4
申请日：1985-03-23
申请人： Takeda Chemical Industries, Ltd.
发明人： Senoo, Masaharu , Onda, Haruo , Igarashi, Koichi
IPC分类号： C12N15/00 , C12P19/34 , C12P21/02 , C07K7/10 , C07K15/26
CPC分类号： C07K14/55 , C07K14/57 , C07K2319/00 , C07K2319/75
摘要： A polypeptide comprising amino acid sequence of human interferon and that of human interleukine-2-may be produced by making new DNA by the ligation of DNA containing the structural gene coding for the peptide of human interferon with DNA containing the structural gene coding for human interleukine-2, transforming the host cells with the DNA, and cultivating the transformant.
摘要翻译：包含人干扰素的氨基酸序列和人白细胞介素-2的多肽可以通过将含有编码人干扰素肽的结构基因的DNA与含有编码人白细胞介素的结构基因的DNA -2，用DNA转化宿主细胞，并培养转化体。

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