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1. 发明公开

EP3187598A1 METHOD FOR MODIFYING NUCLEIC ACIDS 有权
标题翻译：修饰核酸的方法
公开(公告)号：EP3187598A1
公开(公告)日：2017-07-05
申请号：EP17155621.0
申请日：2012-01-20
申请人： Takara Bio Inc.
发明人： Yamamoto, Junko , Kubo, Keiko , Uemori, Takashi , Mukai, Hiroyuki , Asada, Kiyozo
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12N15/01 , C12Q1/6848 , C12Q1/686 , C12Q1/689 , C12Q2523/101 , C12Q2525/117
摘要： The present invention pertains to: a method for modifying a nucleic acid contained in a sample, said method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, said method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.
摘要翻译：本发明涉及修饰样品中所含核酸的方法，所述方法包括在酸性多糖和/或核苷酸存在下使样品与核酸修饰剂接触的步骤; 所述方法包括以下步骤：（a）根据用于修饰包含在所述样品中的核酸的方法对样品中包含的核酸进行修饰的步骤; 样品，其包括在酸性多糖和/或核苷酸存在下使样品与核酸修饰剂接触的步骤; 和（b）在步骤（a）之后选择性检测来自样品的未修饰核酸的步骤。本发明还涉及用于这些方法的试剂盒和组合物。

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