专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

EP2570480B1 METHOD FOR SYNTHESIZING CDNA 有权转让
公开(公告)号：EP2570480B1
公开(公告)日：2018-07-04
申请号：EP11780567.1
申请日：2011-05-09
申请人： Takara Bio Inc.
发明人： NAKABAYASHI, Yuko , UEMORI, Takashi , MUKAI, Hiroyuki , ASADA, Kiyozo
IPC分类号： C12N15/00 , C12N15/09 , C12Q1/68 , C12N15/10
CPC分类号： C12N15/1096 , C12P19/34
摘要： A method for synthesizing cDNA characterized by preparing a reaction solution that does not allow an endodeoxyribonuclease to show its activity, without thermal deactivation of the endodeoxyribonuclease or removal of the endodeoxyribonuclease, and carrying out a reverse transcription reaction, wherein the reaction solution contains a treated sample and a reverse transcriptase, the treated sample being formed by treating a sample comprising RNA and DNA with the endodeoxyribonuclease to degrade DNA in the sample. The method and the kit for synthesizing cDNA of the present invention are widely useful in genetic engineering fields.

2. 发明授权

EP1225229B1 GENE TRANSFER METHOD 有权
标题翻译：基因转移过程
公开(公告)号：EP1225229B1
公开(公告)日：2011-10-19
申请号：EP00969984.4
申请日：2000-10-23
申请人： TAKARA BIO INC.
发明人： UENO, Takashi , MATSUMURA, Hajime , TANAKA, Keiji , IWASAKI, Tomoko , UENO, Mitsuhiro , FUJINAGA, Kei , ASADA, Kiyozo , KATO, Ikunoshin
IPC分类号： C12N15/861 , A61K35/12 , A61K35/76 , A61K48/00 , C12N5/10 , C12N7/01
CPC分类号： C12N15/86 , C12N2710/10343 , C12N2800/30
摘要： A method of transferring a foreign gene into cells, characterized by involving: the step of transferring into the cells with the use of an adenovirus vector, a first nucleic acid, which has a sequence provided with adeno-associated virus-origin ITRs in both sides of the target foreign gene to be transferred, and a second nucleic acid, which has an adeno-associated virus-origin rep gene and a promoter for expressing this gene and carries a stuffer sequence inserted thereinto sandwiched in two recombinase recognition sequences and located between the rep gene and the promoter; and the step of expressing the Rep protein under the action of recombinase in the cells obtained in the above step to thereby integrate the target foreign gene into the chromosomal DNA.

3. 发明公开

EP1947177A1 NOVEL ENDORIBONUCLEASE 审中-公开
标题翻译：新型ENDORIBONUCLEASE
公开(公告)号：EP1947177A1
公开(公告)日：2008-07-23
申请号：EP06797712.4
申请日：2006-09-08
申请人： Takara Bio, Inc.
发明人： SHIMADA, Masamitsu , TAKAYAMA, Masanori , ASADA, Kiyozo , KATO, Ikunoshin
IPC分类号： C12N15/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/22 , C12N15/09 , C12P19/34
CPC分类号： C12N9/22
摘要： A novel endoribonuclease activity exhibiting polypeptide; a nucleic acid coding for the polypeptide; a recombinant DNA comprising the nucleic acid; a transformant obtained by transformation using the recombinant DNA; a process for producing the polypeptide, characterized by including the steps of culturing the transformant and collecting the polypeptide from the culture; a process for producing single-strand RNA fragments, characterized by including the step of causing the polypeptide to act on a single-strand RNA; and a method of fragmenting a single-strand RNA.
摘要翻译：一种新的显示多肽的内切核糖核酸酶活性; 编码多肽的核酸; 包含该核酸的重组DNA; 通过使用重组DNA转化获得的转化体; 一种生产多肽的方法，其特征在于包括以下步骤：培养转化体并从培养物中收集多肽; 生产单链RNA片段的方法，其特征在于包括使多肽作用于单链RNA的步骤; 以及将单链RNA片段化的方法。

4. 发明公开

EP1921139A1 NOVEL ENDORIBONUCLEASE 审中-公开
标题翻译：新型ENDORIBONUCLEASE
公开(公告)号：EP1921139A1
公开(公告)日：2008-05-14
申请号：EP06767803.7
申请日：2006-07-04
申请人： Takara Bio, Inc.
发明人： SHIMADA, Masamitsu , TAKAYAMA, Masanori , ASADA, Kiyozo , KATO, Ikunoshin
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/22 , C12P19/30
CPC分类号： C12N9/22
摘要： A polypeptide having a novel endoribonuclease activity; a nucleic acid encoding the polypeptide; recombinant DNA having the nucleic acid therein; a transformant transformed with the recombinant DNA; a process for producing the polypeptide comprising the steps of cultivating the transformant and collecting the polypeptide from the culture; a process for producing a digest of single-stranded RNA comprising the step of reacting the polypeptide with the single-stranded RNA; and a method for the digestion of single-stranded RNA.
摘要翻译：具有新的内切核糖核酸酶活性的多肽; 编码该多肽的核酸; 其中具有核酸的重组DNA; 用重组DNA转化的转化体; 一种生产多肽的方法，包括以下步骤：培养转化体并从培养物中收集多肽; 生产单链RNA消化物的方法，包括使多肽与单链RNA反应的步骤; 和消化单链RNA的方法。

5. 发明授权

EP0994191B1 SYSTEM FOR EXPRESSING HYPERTHERMOSTABLE PROTEASE 失效
标题翻译：系统用于表达一个超热稳定性蛋白酶
公开(公告)号：EP0994191B1
公开(公告)日：2006-03-01
申请号：EP98923114.7
申请日：1998-06-04
申请人： TAKARA BIO INC.
发明人： TAKAKURA, Hikaru , MORISHITA, Mio , SHIMOJO, Tomoko , ASADA, Kiyozo , KATO, Ikunoshin
IPC分类号： C12N15/57 , C12N9/52 , C12P21/02
CPC分类号： C12N15/75 , C07K2319/02 , C12N9/52
摘要： A hyperthermostable protease having the amino acid sequence represented by the SEC ID NO:1 of the Sequence Listing or a sequence derived therefrom by deletion, substitution, insertion or addition of one to several amino acid residues, a gene encoding the hyperthermostable protease, and a process for preparing the protease, aiming at providing by genetic engineering techniques a hyperthermophile protease which is advantageous for industrial use.

6. 发明公开

EP1331275A1 METHOD OF DETERMINING NUCLEIC ACID BASE SEQUENCE 审中-公开
标题翻译： VERFAHREN ZUR BESTIMMUNG EINERNUKLEINSÄUREBASENSEQUENZ
公开(公告)号：EP1331275A1
公开(公告)日：2003-07-30
申请号：EP01976869.6
申请日：2001-10-30
申请人： TAKARA BIO INC.
发明人： UEMORI, Takashi , YAMASHITA, Hiroshige , HOKAZONO, Shigekazu 205, Takara , SATO, Yoshimi , MUKAI, Hiroyuki , ASADA, Kiyozo , KATO, Ikunoshin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869
摘要： A method of determining the base sequence of a nucleic acid characterized by involving: the step of amplifying a template nucleic acid in the presence of at least two primers each having a tag sequence, a primer specific to the template nucleic acid and a DNA polymerase, wherein the primers having tag sequences have each the tag sequence in the 5'-terminal side thereof and a specific base sequence consisting of three or more nucleotides in the 3'-terminal side; and the step of directly sequencing the amplified fragments obtained in the above step.
摘要翻译：一种确定核酸碱基序列的方法，其特征在于包括：在存在至少两个具有标签序列的引物，对模板核酸特异的引物和DNA聚合酶的引物的情况下扩增模板核酸的步骤，其中具有标签序列的引物各自具有其5'末端侧的标记序列和3'-末端侧的3个以上核苷酸组成的特异碱基序列; 以及对上述步骤中获得的扩增片段进行直接测序的步骤。

7. 发明授权

EP0930361B1 CELL COMPOSITIONS 失效
标题翻译： ZELLZUSAMMENSTELLUNGEN
公开(公告)号：EP0930361B1
公开(公告)日：2003-06-11
申请号：EP97928515.2
申请日：1997-06-30
申请人： TAKARA BIO INC.
发明人： ASADA, Kiyozo , KONISHI, Haruko , KOYAMA, Nobuto , KATO, Ikunoshin
IPC分类号： C12N5/00 , C12N5/06
CPC分类号： C12N5/0093 , C12N5/0647 , C12N2501/125 , C12N2501/23 , C12N2501/90

8. 发明公开

EP2669385A1 METHOD FOR MODIFYING NUCLEIC ACIDS 有权转让
标题翻译： VERFAHRENFÜRNUKLEINSÄUREMODIFIKATION
公开(公告)号：EP2669385A1
公开(公告)日：2013-12-04
申请号：EP12739127.4
申请日：2012-01-20
申请人： Takara Bio, Inc.
发明人： YAMAMOTO, Junko , KUBO, Keiko , UEMORI, Takashi , MUKAI, Hiroyuki , ASADA, Kiyozo
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12N15/01 , C12Q1/6848 , C12Q1/686 , C12Q1/689 , C12Q2523/101 , C12Q2525/117
摘要： The present invention pertains to: a method for modifying a nucleic acid contained in a sample, said method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, said method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.
摘要翻译：本发明涉及：用于修饰样品中所含核酸的方法，所述方法包括使样品与酸性多糖和/或核苷酸存在下与核酸改性剂接触的步骤; 以及选择性地检测来自样品中含有的活细胞的核酸的方法，所述方法包括以下步骤：（a）根据包含在所述样品中的核酸的方法修饰样品中的核酸的步骤样品，其包括在酸性多糖和/或核苷酸的存在下使样品与核酸改性剂接触的步骤; 和（b）在步骤（a）之后从样品中选择性地检测未修饰的核酸的步骤。本发明还涉及用于这些方法的试剂盒和组合物。

9. 发明授权

EP0870839B1 METHOD FOR GENE INTRODUCTION INTO TARGET CELLS BY RETROVIRUS 失效
标题翻译：的程序对靶细胞的基因通过逆转录病毒手段的简介
公开(公告)号：EP0870839B1
公开(公告)日：2006-05-10
申请号：EP96937515.3
申请日：1996-11-07
申请人： TAKARA BIO INC.
发明人： ASADA, Kiyozo , UEMORI, Takashi , UENO, Takashi , KOYAMA, Nobuto , HASHINO, Kimikazu , KATO, Ikunoshiu
IPC分类号： C12N15/86 , C12N15/12 , C12N5/10
CPC分类号： C12N15/86 , A61K48/00 , C07K14/505 , C07K14/78 , C07K2319/00 , C12N9/1088 , C12N2740/13043 , C12N2740/13045
摘要： A method for elevating the gene introduction efficiency into target cells by a retrovirus which comprises infecting the target cells with the retrovirus in the presence of a mixture of an effective amount of a functional substance having a retrovirus-binding site with an effective amount of another functional substance having a target cell-binding site, or in the presence of an effective amount of a functional substance having both of these binding sites in a single molecule. The functional substances are optionally immobilized on beads, etc. This method is usable in, for example, gene therapy.

10. 发明授权

EP0818532B1 PLANT PROMOTER AND GENE EXPRESSION WITH THE USE OF THE SAME 失效
标题翻译：植物启动子及其用途的基因表达
公开(公告)号：EP0818532B1
公开(公告)日：2005-11-16
申请号：EP96906945.9
申请日：1996-03-26
申请人： TAKARA BIO INC.
发明人： OBA, Toshiharu , TAKAHASHI, Syuichi , ANMA, Yoshiko , ASADA, Kiyozo , KATO, Ikunoshin
IPC分类号： C12N15/11 , C12N15/82 , C12N5/10 , A01H5/00 , C12P21/02 , C12N9/10
CPC分类号： C12N15/8223 , C12N9/1051
摘要： A plant promoter capable of inducing the expression specifically at the site and time wherein the reconstitution of plant cell wall xyloglucan is necessary, namely, a plant promoter originating in a gene which encodes an endo-xyloglucan transferase or a gene which encodes a substance having a function equivalent thereto; and a method for altering the function of a plant with the use of the plant promoter and a method for cloning the plant promoter.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式