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1. 发明公开

EP3968004A1 CELL IMAGING DEVICE, CELL IMAGING METHOD, AND SAMPLE CELL 审中-实审
公开(公告)号：EP3968004A1
公开(公告)日：2022-03-16
申请号：EP21206431.5
申请日：2015-12-25
申请人： SYSMEX CORPORATION
发明人： Fukuda, Masakazu , Tanaka, Masamichi , Liu, Yanyan , Fujii, Ryosuke , Inutsuka, Takumi , Tsukahara, Tomoyuki
IPC分类号： G01N21/17 , G01N15/00 , G01N15/14 , G01N21/01 , G01N33/483 , G01N33/493 , G02B21/26 , G02B21/36 , G02B21/30
摘要： Provided are a cell imaging device and a cell imaging method that can shorten the time period of taking images of cells in a liquid sample, compared with conventional techniques. This cell imaging device 100 introduces a urine sample containing cells into an internal space 11 of a sample cell 10, moves in a second direction at least one of the sample cell 10 and an objective lens 52 while at least one of the sample cell 10 and the objective lens 52 is moved in a first direction, the second direction being different from the first direction, and takes, at a plurality of imaging positions, images of cells contained in the urine sample by means of an imaging unit 50.

2. 发明公开

EP3076158A3 CELL IMAGING APPARATUS AND CELL IMAGING METHOD 有权
标题翻译： CELL插图DEVICE AND CELL例证程序
公开(公告)号：EP3076158A3
公开(公告)日：2016-12-21
申请号：EP16161362.5
申请日：2016-03-21
申请人： SYSMEX CORPORATION
发明人： Fukuda, Masakazu , Liu, Yanyan , Ootani, Toshihiro
IPC分类号： G01N15/14 , G01N15/00 , G01N15/10 , G01N33/493
摘要： A cell imaging apparatus (100) captures images of cells contained in a liquid specimen comprising: an image capture unit (50) including an objective lens (52), specimen cells (10a and 10b) each including an inner space (11) which is capable of holding a liquid specimen and which is elongated in one direction, the specimen cells arranged such that the inner spaces are aligned in a row in a longitudinal direction of the inner spaces, a drive unit (61, 62) that moves at least one of a)one or more specimen cells; and b)the objective lens; and a controller (70) that controls the drive unit to move at least one of: a)one or more specimen cells; and b)the objective lens in the longitudinal direction and controls the image capture unit to capture images of cells contained in a liquid specimen held in the inner space of each of the specimen cells at multiple image capture positions.

3. 发明公开

EP3570041A1 SAMPLE PREPARATION APPARATUS AND SAMPLE PREPARATION METHOD, AND CELL ANALYZER AND CELL ANALYSIS METHOD 有权
公开(公告)号：EP3570041A1
公开(公告)日：2019-11-20
申请号：EP19183814.3
申请日：2009-03-26
申请人： SYSMEX CORPORATION
发明人： Fukuda, Masakazu , Nagaoka, Kanako , Ishisaka, Masaki
IPC分类号： G01N35/00 , G01N1/10 , G01N1/28 , G01N15/14
摘要： A preprocessing step is performed in which a concentration information of a cell C1 I in a biological sample is acquired by optically measuring a predetermined cell C1 contained in the biological sample flowing in a flow cell 45, and the biological sample is subjected to concentration adjustment of the cell C1 based on the acquired concentration information to prepare a measurement sample. Even when the concentrations of measurement target cells of the respective biological samples are highly varied, measurement samples suitable for the analysis of the measurement target cells can be favorably prepared, and the measurement target cells can be analyzed accurately.

4. 发明公开

EP2045595A3 Cell analyser and cell analysing method 有权
标题翻译：细胞分析仪和细胞分析方法
公开(公告)号：EP2045595A3
公开(公告)日：2013-05-29
申请号：EP08017313.1
申请日：2008-10-01
申请人： SYSMEX CORPORATION
发明人： Fukuda, Masakazu , Ishisaka, Masaki
IPC分类号： G01N15/14 , G01N21/64
CPC分类号： G01N15/147 , G01N15/1434 , G01N15/1459 , G01N2015/1477 , G01N2021/6439
摘要： The present invention is to present a cell analyzer capable of measuring cells which are approximately 20 to 100 µm in size with high precision via flow cytometry. The cell analyzer 10 comprises: a flow cell 51 in which a measurement sample including a measurement target cell flows; a light source part 53 for irradiating a light on the measurement sample flowing in the flow cell 51; an optical system 52 for forming a beam spot on the measurement sample flowing in the flow cell 51, the beam spot having a diameter of 3∼8 µm in a flow direction of the measurement sample and a diameter of 300∼600 µm in a direction perpendicular to the flow direction of the measurement sample; and a light receiving part 55, 58, 59 for receiving a light from the measurement sample.

5. 发明公开

EP1348943A3 Sheath liquid for particle analyzer 审中-公开
标题翻译：用于颗粒分析仪的鞘液
公开(公告)号：EP1348943A3
公开(公告)日：2003-12-17
申请号：EP03006298.8
申请日：2003-03-24
申请人： Sysmex Corporation
发明人： Ozasa, Masatsugu , Inoue, Junya , Fukuda, Masakazu
IPC分类号： G01N15/14
CPC分类号： G01N15/1404 , G01N2015/1409 , G01N2015/1415 , Y10T436/10 , Y10T436/101666 , Y10T436/108331
摘要： A sheath liquid for use with a particle analyzer has a refractive index of 1.3340 or more at the wavelength of sodium D line at 25 °C. A method of using a sheath liquid in which the latter is selected so as to have a refractive index substantially equal to that of the sample containing particles to avoid sporious counts from signals resulting from scattering of light at the boundary between the sheath liquid and the sample fluid. The sheath liquid comprises various compositions including sorfactant and buffer components.
摘要翻译：与粒子分析仪一起使用的鞘液在25℃时在钠D线的波长处具有1.3340或更高的折射率。一种使用鞘液的方法，其中选择后者的折射率与含有样品的颗粒的折射率基本相同，以避免由鞘液和样品之间的边界处的光散射引起的信号引起的臭名昭着的计数流体。鞘液包含各种组合物，包括表面活性剂和缓冲剂组分。

6. 发明公开

EP0949498A2 Apparatus and method for differentiating erythrocytes in urine 有权
标题翻译：尿素中的Vorrichtung und Verfahren zur Unterscheidung von Erythrozyten
公开(公告)号：EP0949498A2
公开(公告)日：1999-10-13
申请号：EP99400842.3
申请日：1999-04-07
申请人： Sysmex Corporation
发明人： Kanai, Kazuyuki , Fukuda, Masakazu
IPC分类号： G01N15/02 , G01N33/493
CPC分类号： G01N15/1429 , G01N15/1459 , G01N33/493 , G01N2015/0065 , G01N2015/1493
摘要： An apparatus for differentiating erythrocytes in urine includes: a sheath flow cell for forming a urine specimen flow by surrounding a urine specimen with a sheath liquid, the urine specimen containing particles; a light application device for applying light to the urine specimen flow; a light detector for detecting a light signal emitted by each particle in the urine specimen flow; an identification device for identifying erythrocytes from the particles in the urine specimen flow in accordance with the detected light signal; a particle size distribution preparing device for preparing a particle size distribution diagram of the identified erythrocytes; a determining device for determining an origin of the erythrocytes in accordance with an erythrocyte size value at which an accumulated frequency of erythrocytes as counted from a smallest size in the particle size distribution diagram agrees with a preset value larger than half of a total accumulated frequency in the particle size distribution diagram; and an output device for outputting a determination result.
摘要翻译：用于分离尿液中的红细胞的装置包括：用鞘液包围尿标本形成尿液样本流的鞘流池，尿液样本含有颗粒; 用于向所述尿液样本流施加光的光施加装置; 光检测器，用于检测尿液样本流中每个颗粒发射的光信号; 用于根据检测到的光信号从尿液样本流中的颗粒中鉴定红细胞的识别装置; 用于制备鉴定的红细胞的粒度分布图的粒度分布准备装置; 用于根据红细胞大小值确定红细胞起源的确定装置，其中在粒度分布图中从最小尺寸计数的红细胞的累计频率与大于总累积频率的一半的预设值一致粒度分布图; 以及用于输出确定结果的输出装置。

7. 发明公开

EP3957991A1 URINE ANALYSIS SYSTEM, IMAGE CAPTURING APPARATUS, CELL IMAGE CAPTURING APPARATUS, URINE ANALYSIS METHOD, MANAGEMENT APPARATUS, AND INFORMATION PROCESSING APPARATUS 审中-实审
公开(公告)号：EP3957991A1
公开(公告)日：2022-02-23
申请号：EP21202127.3
申请日：2016-01-29
申请人： SYSMEX CORPORATION
发明人： Fukuda, Masakazu , Tanaka, Masamichi , Tanaka, Yousuke
IPC分类号： G01N33/493 , G01N15/14 , G01N21/78 , G01N33/483 , G01N33/52 , G06V20/69
摘要： A urine analysis system 10 includes: a testing apparatus 10 that measures particles included in a urine sample according to a flow cytometry method; an image capturing apparatus 20 that captures images of particles in the urine sample to acquire cell images; and a management apparatus 30 that receives a measurement result obtained by the testing apparatus 10 and the cell images acquired by the image capturing apparatus 20. The management apparatus 30 generates an order to capture an image of the urine sample based on the measurement result obtained by the testing apparatus 10. The image capturing apparatus 20 executes the image capturing processing of the particles in the urine sample for which the image capturing order has been generated by the management apparatus 30, and transmits the acquired cell images to the management apparatus 30.

8. 发明授权

EP3076158B1 CELL IMAGING APPARATUS 有权
公开(公告)号：EP3076158B1
公开(公告)日：2019-11-13
申请号：EP16161362.5
申请日：2016-03-21
申请人： SYSMEX CORPORATION
发明人： Fukuda, Masakazu , Liu, Yanyan , Ootani, Toshihiro
IPC分类号： G01N15/14 , G01N15/00 , G01N15/10 , G01N33/493

9. 发明授权

EP0949498B1 Apparatus and method for differentiating erythrocytes in urine 有权
标题翻译：的装置和方法在尿区分红细胞
公开(公告)号：EP0949498B1
公开(公告)日：2005-10-26
申请号：EP99400842.3
申请日：1999-04-07
申请人： SYSMEX CORPORATION
发明人： Kanai, Kazuyuki , Fukuda, Masakazu
IPC分类号： G01N15/02 , G01N33/493 , G01N15/14
CPC分类号： G01N15/1429 , G01N15/1459 , G01N33/493 , G01N2015/0065 , G01N2015/1493

10. 发明公开

EP1348943A2 Sheath liquid for particle analyzer 审中-公开
标题翻译： Umhüllungsstromfüreinen Teilchenanalysator
公开(公告)号：EP1348943A2
公开(公告)日：2003-10-01
申请号：EP03006298.8
申请日：2003-03-24
申请人： Sysmex Corporation
发明人： Ozasa, Masatsugu , Inoue, Junya , Fukuda, Masakazu
IPC分类号： G01N15/14
CPC分类号： G01N15/1404 , G01N2015/1409 , G01N2015/1415 , Y10T436/10 , Y10T436/101666 , Y10T436/108331
摘要： A sheath liquid for use with a particle analyzer has a refractive index of 1.3340 or more at the wavelength of sodium D line at 25 °C.
A method of using a sheath liquid in which the latter is selected so as to have a refractive index substantially equal to that of the sample containing particles to avoid sporious counts from signals resulting from scattering of light at the boundary between the sheath liquid and the sample fluid. The sheath liquid comprises various compositions including sorfactant and buffer components.
摘要翻译：用于粒子分析仪的鞘液在25℃的D线波长处的折射率为1.3340以上。＆lt;使用鞘液的方法，其中后者被选择为具有折射率基本上等于含有颗粒的样品的折射率，以避免由鞘液体和样品流体之间的边界处的光散射引起的信号的计数。鞘液包含各种组合物，包括助溶剂和缓冲组分。

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