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1. 发明公开

EP3071699A4 METHODS FOR PRODUCING DICARBOXYLIC ACIDS 审中-公开
公开(公告)号：EP3071699A4
公开(公告)日：2017-11-22
申请号：EP14863233
申请日：2014-11-20
申请人： SYNTHETIC GENOMICS INC
发明人： GRIFFIN BENJAMIN M , KAMBOURAKIS SPIROS
IPC分类号： C12P7/64 , C12N9/02
CPC分类号： C12P7/6409 , C12N9/001 , C12N9/0071 , C12Y114/14001
摘要： The present invention provides methods of producing dicarboxylic acids. The methods involve incubating a fatty acid or hydrocarbon substrate with an enzyme to produce a dicarboxylic acid product. The enzyme acts on the substrate to produce a product that has been both over-oxidized and has undergone cleavage of a C—C bond. In some embodiments the enzymes having these useful characteristics are mutants of a cytochrome P450 enzyme, for example an enzyme of the class CYP102 or a mutant thereof. The invention provides enzymes where these desirable characteristics can be found in a single enzyme, and thus in some embodiments the methods can be performed through the action of a single enzyme.

2. 发明公开

EP3119899A4 COMPOSITIONS AND METHODS FOR PRODUCING CHEMICALS AND DERIVATIVES THEREOF 审中-公开
公开(公告)号：EP3119899A4
公开(公告)日：2017-11-29
申请号：EP15764217
申请日：2015-03-20
申请人： SYNTHETIC GENOMICS INC
发明人： KAMBOURAKIS SPIROS , GRIFFIN BENJAMIN M , MARTIN KEVIN V
IPC分类号： C12P7/58 , C12N9/04 , C12P7/42 , C12P7/44 , C12P17/04 , C12P17/06
CPC分类号： C12P7/58 , C12P7/44 , C12P17/06
摘要： The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency. Additional products that can be produce include metabolic products such as, but not limited to, guluronic acid, L-iduronic acid, idaric acid, glucaric acid. Any of the products can be produced using glucose as a substrate or using any intermediate in any of the methods or pathways of the invention.

3. 发明公开

EP2898083A4 COMPOSITIONS AND METHODS FOR PRODUCING CHEMICALS AND DERIVATIVES THEREOF 审中-公开
标题翻译： ZUSAMMENSETZUNGEN UND VERFAHREN ZUR HERSTELLUNG VON CHEMIKALIEN UND DERIVATEN DAVON
公开(公告)号：EP2898083A4
公开(公告)日：2016-05-25
申请号：EP13838235
申请日：2013-09-20
申请人： SYNTHETIC GENOMICS INC
发明人： KAMBOURAKIS SPIROS , GRIFFIN BENJAMIN M , MARTIN KEVIN V
IPC分类号： C12P7/58 , C12N9/04
CPC分类号： C12P7/58 , C07D307/68 , C12P17/12
摘要： The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency. Additional products that can be produce include metabolic products such as, but not limited to, guluronic acid, L-iduronic acid, idaric acid, glucaric acid. Any of the products can be produced using glucose as a substrate or using any intermediate in any of the methods or pathways of the invention.
摘要翻译：本发明提供了生产一种或多种酶途径的产物的方法。用于本发明方法的途径涉及一个或多个转化步骤，例如将古罗糖醛酸酶促转化成D-葡糖二酸（步骤7）; 将5-酮葡萄糖酸盐（5-KGA）酶促转化成L-阿魏酸（步骤15）; 步骤7b）中L-赖康酸转化成Idaric酸的酶促转化; 并将5-酮基葡糖酸酯酶促转化成4,6-二羟基2,5-二酮己酸酯（2,5-DDH）（步骤16）。在一些实施方案中，本发明的方法产生作为产物的2,5-呋喃二羧酸（FDCA）。所述方法包括作为步骤的酶和化学转化。还提供了将葡萄糖转化为5-脱氢-4-脱氧葡糖二酸（DDG）和将葡萄糖转化成2,5-呋喃二羧酸（FDCA）的各种途径。该方法还涉及使用以高特异性和高效率进行反应的工程化酶。可以生产的其它产品包括代谢产物，例如但不限于古洛糖醛酸，L-艾杜糖醛酸，id酸，葡糖二酸。可以使用葡萄糖作为底物或使用本发明的任何方法或途径中的任何中间体来生产任何产物。

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