专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

EP1406091B1 METHOD OF ANALYZING PROTEIN OCCURRING IN CELL OR SUBSTANCE INTERACTING WITH THE PROTEIN 有权
标题翻译：程序对蛋白质在细胞中的分析发生的或与蛋白质互动物质
公开(公告)号：EP1406091B1
公开(公告)日：2008-02-20
申请号：EP02738766.1
申请日：2002-06-19
申请人： SUNTORY LIMITED
发明人： NAKAJIMA, Terumi , NAOKI, Hideo , YASUDA, Akikazu , IWASA, Keiko
IPC分类号： G01N33/566 , G01N33/483 , G01N30/72 , G01N27/62 , G01N33/68 , G01N33/50
CPC分类号： C40B30/04 , G01N33/5008 , G01N33/502 , G01N33/5082 , G01N33/566 , G01N33/6803 , G01N33/6845 , G01N2500/00 , G01N2500/20
摘要： A method which comprises: bringing cells having a protein into contact with a substance interacting with the protein to thereby make them to interact; purifying the cells as being under the interaction with the substance; irradiating the purified cells with laser to thereby directly ionize the substance; and analyzing the thus ionized substance by mass spectrometry. Using this method, the molecular weight, molecular chemical species and molecular structure of a protein occurring in cell surface layer or in cells or a substance interacting with the protein can be easily identified or analyzed within a short time. The above method is a novel method of screening a substance interacting with a protein occurring in cells without resort to the conventional bioassay methods. Also, it is a novel method of judging the presence of a specific protein in cells with the use of a substance interacting with the protein. Moreover, these methods can be conveniently performed and enable accurate and quick judgment.

2. 发明公开

EP1179539A1 NOVEL PROTEIN HAVING HEMOLYTIC ACTIVITY AND GENE ENCODING THE PROTEIN 有权
标题翻译：具有溶血活性的新蛋白质和编码蛋白质的基因
公开(公告)号：EP1179539A1
公开(公告)日：2002-02-13
申请号：EP01915654.6
申请日：2001-03-21
申请人： SUNTORY LIMITED
发明人： NAGAI, Hiroshi , KURODA, Kyoko , NAKAJIMA, Terumi
IPC分类号： C07K14/435 , C12P21/02 , C12N1/15 , C12N1/19 , C12N1/21 , A61K38/17 , C12N15/12 , C07K16/18 , C12N5/10 , A61K39/395 , A61P7/02 , A01N63/00
CPC分类号： C07K14/43595 , A61K38/00
摘要： Novel proteins having hemolytic activity giving the new approach of development of the drugs, and having the following property are provided:

(1) having hemolytic activity;
(2) having a molecular weight of about 50,000 Da (determined by SDS gel electrophoresis); and
(3) having an amino acid sequence represented by any of SEQ ID NO 1 and SEQ ID NO 2 as a partial amino acid sequence represented by the following amino acid sequences (1) and (2);

Amino acid sequence (1):
Amino acid sequence (2):
(wherein, an amino acid residue is written by the 3 letters notation def fined by IUPAC and IUB)
obtained from nematocyst of Carybdea alata.
摘要翻译：具有溶血活性的新蛋白质为药物开发提供了新的方法，并具有以下性质：（1）具有溶血活性; （2）分子量约为50,000Da（通过SDS凝胶电泳测定）; （3）具有下述氨基酸序列（1）和（2）所示的部分氨基酸序列的由序列号1和序列号2中任一个表示的氨基酸序列。氨基酸序列（1）：从Carybdea alata的线虫获得的氨基酸序列（2）:(其中，氨基酸残基由IUPAC和IUB定义的3个字母符号表示）。

3. 发明授权

EP1179539B1 PROTEIN HAVING HEMOLYTIC ACTIVITY AND GENE ENCODING THE PROTEIN 有权
标题翻译：具有活性的蛋白质和本蛋白质编码溶血GEN
公开(公告)号：EP1179539B1
公开(公告)日：2007-01-17
申请号：EP01915654.6
申请日：2001-03-21
申请人： SUNTORY LIMITED
发明人： NAGAI, Hiroshi , KURODA, Kyoko , NAKAJIMA, Terumi
IPC分类号： C07K14/435 , C12P21/02 , C12N1/15 , C12N1/19 , C12N1/21 , A61K38/17 , C12N15/12 , C07K16/18 , C12N5/10 , A61K39/395 , A61P7/02 , A01N63/00
CPC分类号： C07K14/43595 , A61K38/00
摘要： A novel protein having a hemolytic activity and providing a new approach to the development of drugs which has the following characteristics: (1) having a hemolytic activity; (2) having a molecular weight of about 50,000 Da (determined by SDS gel electrophoresis); and (3) having an amino acid sequence containing the partial amino acid sequences represented by SEQ ID NOS:1 and 2, i.e., having partial chemical structures of the following amino acid sequences (1) and (2): amino acid sequence (1): Tyr-Arg-Asp-Gln-Glu-Leu-Glu-Asp-Asn-Val-Lys; and amino acid sequence (2): Lys-Trp-Pro-Asp-Tyr-Phe-Val-Tyr-Met-Glu-Ser-Ser- Ala-His-Gly-Tyr-Ile-Arg; Wherein each amino acid residue is expressed in the triplet code as specified by IUPAC or IUB; and is obtained from nematocyst of Carybdea alata .

4. 发明公开

EP1259820A2 METHOD FOR ANALYSIS OF SUBSTANCES IN TISSUE OR IN CELLS 审中-公开
标题翻译：程序对物质在组织或细胞分析
公开(公告)号：EP1259820A2
公开(公告)日：2002-11-27
申请号：EP01908267.6
申请日：2001-03-02
申请人： SUNTORY LIMITED
发明人： YASUDA, Akikazu , YASUDA, Yoshimi , NAKAJIMA, Terumi
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , G01N33/6818 , G01N33/6851
摘要： An improved method for directly identifying a chemical structure of a substance present in tissue or cells of organisms is disclosed. In particularly, the method for directly identifying a chemical structure of a substance present in tissue or cells of various kind of organisms comprises the following steps: (1) a step irradiating a laser to a certain intracellular region of a sample tissue slice or a cell, and analyzing the generating mass ions to obtain mass spectrum of the substance present at the cite, (2) a step analyzing the mass spectrum to obtain a mass profile of the substance existing at the intracellular region of the sample tissue slice or in cell, and(3) a step determining the chemical structure of the substance corresponding a certain molecular weight appeared in the mass profile. The method of this invention is conducted by utilizing the combined techniques of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and on-line capillary reversed-phase HPLC/quadrupole orthogonal acceleration time-of-flight (Q-Tof)-MS, and molecular cloning, is disclosed.

5. 发明授权

EP0916681B1 NEUROPEPTIDES ORIGINATING IN SCORPION 失效
标题翻译：天蝎特异性肽NEURO
公开(公告)号：EP0916681B1
公开(公告)日：2005-10-26
申请号：EP97950385.1
申请日：1997-12-25
申请人： SUNTORY LIMITED
发明人： LEBRUN, Bruno , ROMI-LEBRUN, Régine , MINAKATA, Hiroyuki , NAKAJIMA, Terumi , HASHINO, Junko
IPC分类号： C07K14/435 , A61K38/17
CPC分类号： C07K14/43522 , A61K38/00
摘要： The peptide represented by the following amino acid sequence, having been amidated at the C-end and carrying four disulfide bonds in its molecule: Ala-Ser-Cys-Arg-Thr-Pro-Lys-Asp-Cys-Ala-Asp-Pro-Cys-Arg-Lys-Glu-Thr-Gly-Cys-Pro-Tyr-Gly-Lys-Cys-Met-Asn-Arg-Lys-Cys-Lys-Cys-Asn-Arg-Cys, which inhibits the production of IL-2 by blocking the voltage-dependent K channel and is isolated from the poison gland of a scorpion Heterometrus spinnifer; and peptides analogous thereto. These peptides are neuropeptides which exhibit activities of, at an extremely low concentration, blocking the voltage-dependent K channel and thus inhibiting the IL-2 production and are applicable to drugs against scorpion toxin, etc.

6. 发明公开

EP1067140A1 NOVEL HEMOLYTIC ACTIVE PROTEINS AND GENES ENCODING THE SAME 审中-公开
标题翻译：根据DAFÜRKODIERENDE GENE的NEEM HEMOLYTISCH AKIST PROTEINE
公开(公告)号：EP1067140A1
公开(公告)日：2001-01-10
申请号：EP99910756.8
申请日：1999-03-30
申请人： SUNTORY LIMITED
发明人： NAGAI, Hiroshi , NAKAJIMA, Terumi
IPC分类号： C07K14/435 , C07K14/745 , C12N1/21 , C12N15/12 , A61K38/36 , A01N63/02
CPC分类号： A01N37/46 , A01N63/02 , A61K38/00 , C07K14/43595
摘要： Novel proteins providing the new approach to development of the drugs and pesticides with the use or application of a hemolytic activity, and novel proteins having the following properties and the genes encoding thereof are provided:

(1) having hemolytic activity;
(2) having a molecular weight of about 50,000 Da (determined by SDS gel electrophoresis);
(3) having an amino acid sequence represented by any of SEQ ID NO 1 to SEQ ID NO 3 as a partial amino acid sequence; and
(4) having an amino acid sequence represented by SEQ ID NO 5 as the full amino acid sequence.
摘要翻译：提供了使用或应用溶血活性提供药物和农药开发新方法的新型蛋白质，以及具有以下性质的新蛋白质及其编码基因：（1）具有溶血活性; （2）分子量约50,000Da（通过SDS凝胶电泳测定）; （3）作为部分氨基酸序列具有由SEQ ID NO 1〜SEQ ID NO 3中任一个表示的氨基酸序列; 和（4）具有由SEQ ID NO 5表示的氨基酸序列作为全氨基酸序列。

7. 发明公开

EP1406091A1 METHOD OF ANALYZING PROTEIN OCCURRING IN CELL OR SUBSTANCE INTERACTING WITH THE PROTEIN 有权
标题翻译：程序对蛋白质在细胞中的分析发生的或与蛋白质互动物质
公开(公告)号：EP1406091A1
公开(公告)日：2004-04-07
申请号：EP02738766.1
申请日：2002-06-19
申请人： SUNTORY LIMITED
发明人： NAKAJIMA, Terumi , NAOKI, Hideo , YASUDA, Akikazu , IWASA, Keiko
IPC分类号： G01N33/566 , G01N33/483 , G01N30/72 , G01N27/62
CPC分类号： C40B30/04 , G01N33/5008 , G01N33/502 , G01N33/5082 , G01N33/566 , G01N33/6803 , G01N33/6845 , G01N2500/00 , G01N2500/20
摘要： A method is disclosed for identifying or analyzing, easily and in a short time, the molecular weight, the chemical species of molecules, and the structure of molecules, of a protein present on the superficial layer of cells or in cells, or a substance interacting with the protein. This method comprises the steps of: bringing the cells having the protein and the substance interacting with the protein into contact with each other to cause the substance to interact with the cells; purifying the cells, with the cells and the substance in an interacting state; irradiating the purified cells with a laser to ionize the substance directly; and analyzing the ionized substance by mass spectrometry.
This method is a new method for screening a substance interacting with a protein present in cells, and a new method for determining the presence of a particular protein in cells with the use of a substance interacting with the protein, the new methods not relying on conventional bioassays. These methods are easy to operate and capable of accurate and prompt determination.
摘要翻译：鉴定或分析（M1）的蛋白质发生的细胞中或在细胞表面，或与该蛋白质的物质相互作用，其包括：（a）使与所述物质的细胞; （b）纯化所述细胞; （C）照射所述纯化的细胞用激光直接电离的物质; 和（d）分析通过质谱法（MS）将这样的离子化物质，是新的。因此独立权利要求中包括了：（1）筛选（M2）的物质并与在细胞表面上或在细胞中的蛋白质相互作用，并且具有已知活性，包括：（A）使它们接触; （b）纯化所述细胞; （C）纯化的细胞的物质的直接电离的激光照射; 通过MS离子化物质的和（d）数据; 和（2）筛选（M3）的细胞或组织中表达的蛋白质并与具有已知活性的物质，包括间行为：（a）使它们相互接触; （B）纯化的组织或细胞; （C）纯化的组织或细胞进行直接电离的激光照射; 和由MS离子化物质的（d）中分析，这过程中产生的质谱与那些在运行的对照获得用于判断基于所述相互作用的蛋白质的存在或不存在相比较。

8. 发明公开

EP0916681A1 NEUROPEPTIDES ORIGINATING IN SCORPION 失效
标题翻译： SKORPION-SPEZIFISCHE神经病学
公开(公告)号：EP0916681A1
公开(公告)日：1999-05-19
申请号：EP97950385.1
申请日：1997-12-25
申请人： SUNTORY LIMITED
发明人： LEBRUN, Bruno , ROMI-LEBRUN, Régine , MINAKATA, Hiroyuki , NAKAJIMA, Terumi , HASHINO, Junko
IPC分类号： C07K14/435 , A61K38/17
CPC分类号： C07K14/43522 , A61K38/00
摘要： The invention provides a peptide capable of blocking voltage-gated K + channels and inhibiting IL-2 production, which is represented by an amino acid sequence of:

Ala-Ser-Cys-Arg-Thr-Pro-Lys-Asp-Cys-Ala-Asp-Pro-Cys-Arg-Lys-
Glu-Thr-Gly-Cys-Pro-Tyr-Gly-Lys-Cys-Met-Asn-Arg-Lys-Cys-Lys-
Cys-Asn-Arg-Cys;
in which the C-terminal is amidated and four disulfide bridges are present in the molecule, and their related peptides isolated from the venom gland of a scorpion Heterometrus spinnifer .
The peptides provided by the present invention are neuropeptides which can block voltage-gated K + channels and inhibit IL-2 production at an extremely low concentration and, therefore, are expected to contribute to application of scorpion toxins to medicines and so on.
摘要翻译：本发明提供能够阻断电压门控K +通道并抑制IL-2产生的肽，其由以下氨基酸序列表示：Ala-Ser-Cys-Arg-Thr-Pro-Lys-Asp-Cys -Ala-Asp-Pro-Cys-Arg-Lys-Glu-Thr-Gly-Cys-Pro-Tyr-Gly-Lys-Cys-Met-Asn-Arg-Lys-Cys-Lys-Cys-Asn-Arg-Cys ; 其中C末端被酰胺化并且分子中存在四个二硫键，并且其相关肽从蝎子Heterometrus spinnifer的毒液腺分离。本发明提供的肽是可阻断电压门控K +通道并以极低浓度抑制IL-2产生的神经肽，因此预期有助于将蝎毒素施用于药物等。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式