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2. 发明公开

EP1406091A1 METHOD OF ANALYZING PROTEIN OCCURRING IN CELL OR SUBSTANCE INTERACTING WITH THE PROTEIN 有权
标题翻译：程序对蛋白质在细胞中的分析发生的或与蛋白质互动物质
公开(公告)号：EP1406091A1
公开(公告)日：2004-04-07
申请号：EP02738766.1
申请日：2002-06-19
申请人： SUNTORY LIMITED
发明人： NAKAJIMA, Terumi , NAOKI, Hideo , YASUDA, Akikazu , IWASA, Keiko
IPC分类号： G01N33/566 , G01N33/483 , G01N30/72 , G01N27/62
CPC分类号： C40B30/04 , G01N33/5008 , G01N33/502 , G01N33/5082 , G01N33/566 , G01N33/6803 , G01N33/6845 , G01N2500/00 , G01N2500/20
摘要： A method is disclosed for identifying or analyzing, easily and in a short time, the molecular weight, the chemical species of molecules, and the structure of molecules, of a protein present on the superficial layer of cells or in cells, or a substance interacting with the protein. This method comprises the steps of: bringing the cells having the protein and the substance interacting with the protein into contact with each other to cause the substance to interact with the cells; purifying the cells, with the cells and the substance in an interacting state; irradiating the purified cells with a laser to ionize the substance directly; and analyzing the ionized substance by mass spectrometry.
This method is a new method for screening a substance interacting with a protein present in cells, and a new method for determining the presence of a particular protein in cells with the use of a substance interacting with the protein, the new methods not relying on conventional bioassays. These methods are easy to operate and capable of accurate and prompt determination.
摘要翻译：鉴定或分析（M1）的蛋白质发生的细胞中或在细胞表面，或与该蛋白质的物质相互作用，其包括：（a）使与所述物质的细胞; （b）纯化所述细胞; （C）照射所述纯化的细胞用激光直接电离的物质; 和（d）分析通过质谱法（MS）将这样的离子化物质，是新的。因此独立权利要求中包括了：（1）筛选（M2）的物质并与在细胞表面上或在细胞中的蛋白质相互作用，并且具有已知活性，包括：（A）使它们接触; （b）纯化所述细胞; （C）纯化的细胞的物质的直接电离的激光照射; 通过MS离子化物质的和（d）数据; 和（2）筛选（M3）的细胞或组织中表达的蛋白质并与具有已知活性的物质，包括间行为：（a）使它们相互接触; （B）纯化的组织或细胞; （C）纯化的组织或细胞进行直接电离的激光照射; 和由MS离子化物质的（d）中分析，这过程中产生的质谱与那些在运行的对照获得用于判断基于所述相互作用的蛋白质的存在或不存在相比较。

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