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1. 发明公开

EP2784152A4 METHODS FOR PRODUCING RETINAL TISSUE AND RETINA-RELATED CELL 有权转让
标题翻译：用于生产的视网膜组织及相关的视网膜细胞
公开(公告)号：EP2784152A4
公开(公告)日：2015-11-11
申请号：EP12851901
申请日：2012-11-22
申请人： SUMITOMO CHEMICAL CO , RIKEN
发明人： NAKANO TOKUSHIGE , ANDO SATOSHI , SASAI YOSHIKI , EIRAKU MOTOSUGU
IPC分类号： C12N5/071 , A61K35/30 , A61L27/00 , A61L27/36 , A61L27/38 , C12N5/0789 , C12N5/079 , C12Q1/02 , G01N33/15 , G01N33/50
CPC分类号： A61K35/30 , A61L27/3633 , A61L27/3834 , A61L27/3895 , A61L2430/16 , C12N5/062 , C12N5/0621 , C12N2500/84 , C12N2500/90 , C12N2501/16 , C12N2501/415 , C12N2501/42 , C12N2506/02 , C12N2533/90 , G01N33/5058
摘要： The present invention provides [1] a method for producing a retinal tissue, including the following steps (1) to (3): (1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium containing a substance inhibiting the Wnt signal pathway to form an aggregate of pluripotent stem cells, (2) a second step of subjecting the aggregate formed in the first step to floating culture in a serum-free medium containing a basement membrane preparation, and (3) a third step of subjecting the aggregate cultured in the second step to floating culture in a serum-containing medium; [2] a method for producing an optic-cup-like structure, including a step of subjecting an aggregate containing the retinal tissue cultured in the step (3) above to floating culture in a serum-free medium or serum-containing medium each containing a substance acting on the Sonic hedgehog signal pathway and a substance acting on the Wnt signal pathway; [3] a method for producing a retinal pigment epithelium, including a step of subjecting an aggregate containing the retinal tissue cultured in the step (3) above to floating culture in a serum-free medium or serum-containing medium each containing a substance acting on the Wnt signal pathway (wherein said serum-free medium and serum-containing medium are free of a substance acting on the Sonic hedgehog signal pathway); and [4] a method for producing a retinal layer-specific neural cell, including bringing a retinal progenitor cell contained in a retinal tissue derived from a primate pluripotent stem cell into contact with a substance inhibiting the Notch signal pathway.

2. 发明公开

EP3070162A4 METHOD FOR PRODUCING RETINAL PIGMENT EPITHELIAL CELLS 有权转让
标题翻译： VERFAHREN ZUR HERSTELLUNG VON RETINAPIGMENT-EPITHELZELLEN
公开(公告)号：EP3070162A4
公开(公告)日：2017-05-31
申请号：EP14860084
申请日：2014-10-02
申请人： SUMITOMO CHEMICAL CO , RIKEN
发明人： NAKANO TOKUSHIGE , OZONE CHIKAFUMI , SASAI YOSHIKI
IPC分类号： C12N5/0735 , A61K35/12 , A61K35/44 , A61L27/00 , A61P27/00 , C12N5/074 , C12N5/10
CPC分类号： C12N5/0621 , A61K35/30 , A61K35/545 , A61L27/3813 , A61L27/3834 , A61L27/3895 , A61L2430/16 , C12N5/062 , C12N2501/113 , C12N2501/155 , C12N2501/41 , C12N2501/415 , C12N2501/727 , C12N2506/02 , C12N2506/45
摘要： The present invention provides a method for producing a retinal pigment epithelial cell, including (1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium to form an aggregate of pluripotent stem cells, (2) a second step of subjecting the aggregate formed in step (1) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway and containing a substance acting on the BMP signal transduction pathway, thereby obtaining an aggregate containing retinal progenitor cells, and (3) a third step of subjecting the aggregate formed in step (2) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway and a substance acting on the BMP signal transduction pathway and containing a substance acting on the Wnt signal pathway, thereby obtaining an aggregate containing retinal pigment epithelial cell.
摘要翻译：本发明提供一种视网膜色素上皮细胞的制造方法，其包括（1）使第一步使多能干细胞在无血清培养基中漂浮培养，形成多能干细胞的集合体，（2）第二步骤使步骤（1）中形成的聚集体在无血清培养基或含血清培养基中进行漂浮培养，每种培养基或含血清培养基均不含作用于Sonic hedgehog信号转导途径并含有作用于BMP信号转导途径的物质的物质，从而获得含有视网膜祖细胞的集合体，和（3）将步骤（2）中形成的集合体在不含作用于声波的物质的无血清培养基或含血清培养基中进行漂浮培养的第3步骤刺猬蛋白信号转导途径和作用于BMP信号转导途径并含有作用于Wnt信号途径的物质的物质，从而获得含有视网膜色素上皮细胞。

3. 发明公开

EP3037524A4 METHOD FOR PRODUCING RETINAL TISSUE AND RETINA-RELATED CELLS 有权转让
标题翻译： VERFAHREN ZUR HERSTELLUNG VON NETZHAUTGEWEBE UND RETINAVERWANDTEN ZELLEN
公开(公告)号：EP3037524A4
公开(公告)日：2017-05-03
申请号：EP14838289
申请日：2014-08-22
申请人： SUMITOMO CHEMICAL CO , RIKEN
发明人： NAKANO TOKUSHIGE , OZONE CHIKAFUMI , SASAI YOSHIKI
IPC分类号： C12N5/079 , A61K35/44 , A61L27/00 , A61P27/02 , C12Q1/02 , G01N33/15 , G01N33/50
CPC分类号： C12N5/0621 , A61K35/30 , A61L27/3804 , A61L27/3834 , A61L27/3895 , A61L2430/16 , C12N2500/33 , C12N2500/44 , C12N2500/90 , C12N2501/155 , C12N2501/19 , C12N2501/385 , C12N2501/415 , C12N2501/727 , C12N2506/02 , C12N2506/45
摘要： The present invention provides a method for producing a retinal progenitor cell, including (1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium to form an aggregate of pluripotent stem cells, and (2) a second step of subjecting the aggregate formed in step (1) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway but containing a substance acting on the BMP signal transduction pathway, thereby obtaining an aggregate containing retinal progenitor cells.
摘要翻译：本发明提供一种视网膜祖细胞的制造方法，其包括（1）将多能干细胞在无血清培养基中进行漂浮培养而形成多能干细胞的集合体的第1工序，（2）第2工序使步骤（1）中形成的聚集体在无血清培养基或含血清培养基中进行漂浮培养，每种培养基或含血清培养基均不含作用于Sonic hedgehog信号转导途径但含有作用于BMP信号转导途径的物质的物质，从而获得含有视网膜祖细胞的聚集体。

4. 发明公开

EP2860245A4 METHOD FOR PRODUCING CILIARY MARGINAL ZONE-LIKE STRUCTURE 有权转让
标题翻译： VERFAHREN ZUR HERSTELLUNG EINER ZILIARRANDZONENARTIGEN STRUKTUR
公开(公告)号：EP2860245A4
公开(公告)日：2015-12-23
申请号：EP13800106
申请日：2013-06-07
申请人： SUMITOMO CHEMICAL CO , RIKEN
发明人： NAKANO TOKUSHIGE , ANDO SATOSHI , SASAI YOSHIKI , EIRAKU MOTOTSUGU
IPC分类号： C12N5/079 , A61K35/12 , A61K35/30 , A61L27/00 , A61L27/36 , A61L27/38 , C12N5/0735 , C12N5/10
CPC分类号： C12N5/0621 , A61K35/30 , A61L27/3604 , A61L27/3895 , C12N2500/90 , C12N2501/41 , C12N2501/415 , C12N2501/727 , C12N2506/02 , C12N2506/08
摘要： The present invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure, including a step of culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more of the tissue in a serum-free medium or serum-containing medium each containing a substance acting on the Wnt signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the "cell aggregate in which a RPE65 gene expressing cell does not appear" thus obtained in a serum-free medium or serum-containing medium each not containing a substance acting on the Wnt signal pathway and so on. According to the present invention, a ciliary marginal zone-like structure can be produced with high efficiency.
摘要翻译：本发明提供一种含有睫状边缘区域状结构的细胞聚集体的制造方法，其特征在于，包括培养含有所述组织20％以上的Chx10阳性细胞的视网膜组织的细胞集合体的步骤在仅含有表达RPE65基因的细胞出现前一段时间内含有作用于Wnt信号通路的物质的无血清培养基或含血清培养基中，然后培养“表达RPE65基因的细胞的细胞集合体不出现“，因此在不含有作用于Wnt信号通路的物质等的无血清培养基或含血清培养基中得到。根据本发明，可以高效率地制造睫状边缘区域状结构。

5. 发明公开

EP2784154A4 METHOD OF CRYOPRESERVATION OF TISSUE DERIVED FROM PLURIPOTENT STEM CELLS 有权转让
标题翻译：组织冷冻保存从多能干细胞的方法
公开(公告)号：EP2784154A4
公开(公告)日：2015-05-27
申请号：EP12851379
申请日：2012-11-22
申请人： SUMITOMO CHEMICAL CO , RIKEN
发明人： ANDO SATOSHI , NAKANO TOKUSHIGE , SASAI YOSHIKI , EIRAKU MOTOTSUGU
IPC分类号： C12N5/074 , A01N1/02 , C12N1/04 , C12N5/0735 , C12N5/0775 , C12N5/0789 , C12N5/0797
CPC分类号： A01N1/0221 , C12N1/04
摘要： The present invention provides a method of cryopreserving a tissue derived from a pluripotent stem cell, including the following (1) to (3): (1) a first step of bringing a tissue derived from a pluripotent stem cell into contact with a cell protection solution that contains sulfoxide and chain polyol, (2) a second step of maintaining, in a cryopreservation solution, the pluripotent stem cell-derived tissue that was brought into contact with the cell protection solution in the first step, and, (3) a third step of cryopreserving, in the presence of a coolant, the pluripotent stem cell-derived tissue that was maintained in the cryopreservation solution in the second step. According to the method of the present invention, a stable preservation method of a tissue derived from a pluripotent stem cell can be provided.

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