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1. 发明公开

EP3253867A1 POLYMERASE VARIANTS 有权
标题翻译：聚合酶变体
公开(公告)号：EP3253867A1
公开(公告)日：2017-12-13
申请号：EP16703461.0
申请日：2016-02-01
申请人： F. Hoffmann-La Roche AG , Roche Diagnostics GmbH
发明人： AYER, Aruna , BIBILLO, Arkadiusz , ECKERT, Barbara , SUKO, Shawn , ARNOLD, Cleoma Renetta , SCHWAB, Charles Wayan , THAI, Eileen , LEDERMAN, Ilya , MCGAW, Colin Alexander , SCHULTZ, Tyler O'Brien , BOENITZ-DULAT, Mara , WOERSDOERFER, Bigna , WUNDERLICH, David Daniel
IPC分类号： C12N9/00
摘要： Described herein are variant pol6 polymerase having at least having at least two substitutions, wherein at least one of the substitutions is at position S366 of SEQ ID NO: 2. The disclosed polymerases may be coupled to alpha-hemolysin nanopores, and may be used in nanopore-based sequencing apparatuses.

2. 发明授权

EP3253867B1 POLYMERASE VARIANTS 有权
公开(公告)号：EP3253867B1
公开(公告)日：2020-05-06
申请号：EP16703461.0
申请日：2016-02-01
申请人： F. Hoffmann-La Roche AG , Roche Diagnostics GmbH
发明人： AYER, Aruna , BIBILLO, Arkadiusz , ECKERT, Barbara , SUKO, Shawn , ARNOLD, Cleoma Renetta , SCHWAB, Charles Wayan , THAI, Eileen , LEDERMAN, Ilya , MCGAW, Colin Alexander , SCHULTZ, Tyler O'Brien , BOENITZ-DULAT, Mara , WOERSDOERFER, Bigna , WUNDERLICH, David Daniel
IPC分类号： C12N9/00

3. 发明授权

EP1802748B1 THERMOSTABLE MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT GLUCOSE DEHYDROGENASE 有权转让
标题翻译： THERMO稳定的突变体吡咯CHINON依赖葡萄糖脱氢酶
公开(公告)号：EP1802748B1
公开(公告)日：2011-06-01
申请号：EP05792997.8
申请日：2005-10-14
申请人： Roche Diagnostics GmbH , F. Hoffmann-La Roche AG
发明人： BOENITZ-DULAT, Mara , KRATZSCH, Peter , SCHMUCK, Rainer
IPC分类号： C12N9/04 , C12N15/53 , C12Q1/32
CPC分类号： C12N9/0006
摘要： The present invention relates to a mutant protein of PQQ-dependent s-GDH characterized in that in at least one of the positions 122 and 124 the amino acid lysine is present, wherein these positions correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence (SEQ ID NO: 2), it also discloses genes encoding such mutant s-GDH, and different applications of these sGDH mutants, particularly for determining the concentration of glucose in a sample.

4. 发明公开

EP3516053A2 POL6 POLYMERASE VARIANTS 审中-公开
公开(公告)号：EP3516053A2
公开(公告)日：2019-07-31
申请号：EP17781021.5
申请日：2017-09-20
申请人： F. Hoffmann-La Roche AG , Roche Diagnostics GmbH
发明人： BOENITZ-DULAT, Mara , SUKO, Shawn , AYER, Aruna , WOERSDOERFER, Bigna , ECKERT, Barbara , SARVABHOWMAN, Preethi , SCHWAB, Charles Wayan , WUNDERLICH, David , CLEOMA, Arnold , LEDERMAN, Ilya , THAI, Eileen , MCGAW, Colin , AMATO, Evan
IPC分类号： C12N9/12

5. 发明公开

EP3394252A1 MUTANT 3-HYDROXYBUTYRATE DEHYDROGENASE FROM ALCALIGENES FAECALIS AS WELL AS METHODS AND USES INVOLVING THE SAME 有权
公开(公告)号：EP3394252A1
公开(公告)日：2018-10-31
申请号：EP16826037.0
申请日：2016-12-21
申请人： F. Hoffmann-La Roche AG , Roche Diagnostics GmbH , Roche Diabetes Care GmbH
发明人： KRATZSCH, Peter , BOENITZ-DULAT, Mara , BECK, Daniela , STREIDL, Thomas , HUNT DUVALL, Stacy
IPC分类号： C12N9/04
CPC分类号： C12N9/0006 , C12Q1/32 , C12Q1/54 , C12Y101/0103
摘要： The present disclosure relates to a mutant 3-hydroxybutyrate dehydrogenase (3-HBDH) with improved performance relative to the wild-type 3-HBDH, a nucleic acid encoding the mutant 3-HBDH, a cell having the mutant 3-HBDH or the nucleic acid, and/or a method of determining the amount or concentration of 3-hydroxybutyrate in a sample. Also disclosed is the use of the mutant 3-HBDH for determining the amount or concentration of 3-hydroxybutyrate in a sample, and a device for determining the amount or concentration of 3-hydroxybutyrate in a sample.

6. 发明公开

EP4222257A1 BACTERIAL PILUS PROTEIN COMPLEX FIMGT-DSF STABILIZED PROTEIN COMPLEXES FOR PRODUCING FILAMENTOUS PHAGES 审中-实审
公开(公告)号：EP4222257A1
公开(公告)日：2023-08-09
申请号：EP21786129.3
申请日：2021-09-28
申请人： F. Hoffmann-La Roche AG , Roche Diagnostics GmbH
发明人： BOENITZ-DULAT, Mara , SCHRAEML, Michael , WOERSDOERFER, Bigna
IPC分类号： C12N15/10 , C07K14/245

7. 发明授权

EP3414325B1 MUTANT 3-HYDROXYBUTYRATE DEHYDROGENASE FROM RHODOBACTER SPHAEROIDES AS WELL AS METHODS AND USES INVOLVING THE SAME 有权
公开(公告)号：EP3414325B1
公开(公告)日：2020-01-29
申请号：EP17704245.4
申请日：2017-02-09
申请人： Roche Diabetes Care GmbH , Roche Diagnostics GmbH , F. Hoffmann-La Roche AG
发明人： BECK, Daniela , BOENITZ-DULAT, Mara , KRATZSCH, Peter , STREIDL, Thomas , HORN, Carina
IPC分类号： C12P7/18 , C12N9/04 , C12Q1/32

8. 发明公开

EP2010649A1 IMPROVED MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT SOLUBLE GLUCOSE DEHYDROGENASE 有权转让
标题翻译： VERBESSERTE MUTANTE MORANTEN PYRROLOCHINOLIN-CHINON-ABHÄNGIGERLÖSLICHERGLUCOSE-DEHYDROGENASE
公开(公告)号：EP2010649A1
公开(公告)日：2009-01-07
申请号：EP07724148.7
申请日：2007-04-11
申请人： Roche Diagnostics GmbH , F. Hoffmann-La Roche AG
发明人： BOENITZ-DULAT, Mara , BECK, Daniela , KRATZSCH, Peter , SCHMUCK, Rainer , VON DER ELTZ, Herbert
IPC分类号： C12N9/04
CPC分类号： C12N9/0006
摘要： The present invention discloses a mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alanine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.
摘要翻译：与麦芽糖相比，PQQ依赖性可溶性葡萄糖脱氢酶（s-GDH; EC 1.1.5.2）的突变体具有改善的葡萄糖特异性，其具有通过甘氨酸，丙氨酸或丝氨酸取代位置348处的苏氨酸，其中所述突变体还包含至少一个用于改善突变体的稳定性的突变和用于改善突变体对葡萄糖的亲和力的一个或多个突变，和/或用于进一步提高突变体的特异性的一个或多个突变用于与麦芽糖相比的葡萄糖，并且其中位置348对应于从A.calcoaceticus s-GDH野生型序列已知的氨基酸位置。还公开了编码这种突变体s-GDH的基因以及这些s-GDH突变体的不同应用，特别是用于测定样品中葡萄糖浓度。

9. 发明公开

EP3353314A1 TRANSAMIDATION EMPLOYING SORTASE A IN DEEP EUTECTIC SOLVENTS 审中-公开
公开(公告)号：EP3353314A1
公开(公告)日：2018-08-01
申请号：EP16775565.1
申请日：2016-09-22
申请人： F. Hoffmann-La Roche AG
发明人： SCHATTE, Martin , BOENITZ-DULAT, Mara
IPC分类号： C12P21/02
CPC分类号： C12P21/02
摘要： Herein is reported a method for the enzymatic production of a polypeptide comprising the step of incubating i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 01, wherein X can be any amino acid residue) or LPXTA (SEQ ID NO: 41, wherein X can be any amino acid residue), ii) a second polypeptide that has i) a glycinyl, an alaninyl, or a cysteinyl compound at its N- terminus, or ii) an oligoglycine, or oligoalanine, or a cysteine amino acid residue followed by one to three glycine or alanine amino acid residues at its N-terminus, or iii) a lysine amino acid residue within its 5 N-terminal amino acid residues, and iii) a third polypeptide with sortase A activity, in a deep eutectic solvent and thereby producing a polypeptide.

10. 发明公开

EP3714046A1 PROLINE HYDROXYLASES AS WELL AS USES, METHODS AND PRODUCTS INVOLVING THE SAME 审中-公开
公开(公告)号：EP3714046A1
公开(公告)日：2020-09-30
申请号：EP18807288.8
申请日：2018-11-20
申请人： F. Hoffmann-La Roche AG
发明人： BOENITZ-DULAT, Mara , IDING, Hans , WETZL, Dennis , HINZMANN, Alessa
IPC分类号： C12N9/02

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