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1. 发明公开

EP3174998A1 METHOD FOR SELECTING A TARGET NUCLEIC ACID SEQUENCE 有权转让
标题翻译：用于选择目标核酸序列的方法
公开(公告)号：EP3174998A1
公开(公告)日：2017-06-07
申请号：EP15744928.1
申请日：2015-07-31
申请人： Olink Bioscience AB
发明人： LANDEGREN, Ulf , NONG, Yuan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6853 , C12Q2521/501 , C12Q2525/131 , C12Q2525/161 , C12Q2525/301 , C12Q2531/125 , C12Q2533/101 , C12Q2533/107
摘要： The present invention relates to a method for selecting a target region of interest (ROI) in a target nucleic acid molecule using a nucleic acid probe comprising a 3' sequence capable of hybridising to a target nucleic acid molecule and acting as a primer for the production of a complement of the target ROI (i.e. by target templated extension of the primer), and a sequence capable of templating the circularisation and ligation of the extended probe comprising the reverse complement of the target ROI and a portion of the probe. The circularised molecule thus obtained contains the reverse complement of the target ROI and may be subjected to further analysis and/or amplification etc. The probe may be provided as an oligonucleotide comprising a stem-loop structure or as a partially double- stranded construct and comprises a single-stranded 3' end region containing the target- binding site. A second binding site provided in the probe serves as the ligation template for circularisation, and the stem-loop structure, if present, is cleaved to render the second binding site available for hybridisation to the target complement. Also provided are probes and kits for carrying out such a method.
摘要翻译：本发明涉及使用包含能够与靶核酸分子杂交的3'序列并充当产生的引物的核酸探针选择靶核酸分子中的目标靶区域（ROI）的方法（即通过引物的目标模板延伸），以及能够模板扩增探针的环化和连接的序列，所述扩增探针包含目标ROI和部分探针的反向互补物。如此获得的环化分子含有目标ROI的反向互补物并且可以进行进一步分析和/或扩增等。探针可以作为包含茎环结构或部分双链构建体的寡核苷酸提供，并且包含含有靶结合位点的单链3'端区域。探针中提供的第二结合位点用作环化的连接模板，并且茎环结构（如果存在的话）被切割以使得第二结合位点可用于与靶补体杂交。还提供了用于实施这种方法的探针和试剂盒。

2. 发明授权

EP2920324B1 LOCALISED RCA-BASED AMPLIFICATION METHOD 有权转让
标题翻译：局部化的基于RCA的放大方法
公开(公告)号：EP2920324B1
公开(公告)日：2017-12-27
申请号：EP13798603.0
申请日：2013-11-14
申请人： Olink Bioscience AB
发明人： LANDEGREN, Ulf , CHEN, Lei , WU, Di , NONG, Yuan , GALLANT, Caroline
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q1/6804 , C12Q1/682 , C12Q2531/125 , C12Q2537/149 , C12Q2533/107 , C12Q2525/131 , C12Q2525/301 , C12Q2525/155

3. 发明授权

EP2920320B1 RCA REPORTER PROBES AND THEIR USE IN DETECTING NUCLEIC ACID MOLECULES 有权
标题翻译： RCA记者探测器及其使用的检测NUCLEIC分子的
公开(公告)号：EP2920320B1
公开(公告)日：2016-12-28
申请号：EP13791997.3
申请日：2013-11-14
申请人： Olink Bioscience AB
发明人： LANDEGREN, Ulf , CHEN, Lei , WU, Di , NONG, Yuan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6804 , C12Q1/6816 , C12Q1/6848 , C12Q1/6853 , C12Q2521/501 , C12Q2525/131 , C12Q2525/301 , C12Q2525/307 , C12Q2531/125 , C12Q2521/531

4. 发明授权

EP3174997B1 METHOD FOR SELECTING A TARGET NUCLEIC ACID SEQUENCE 有权
公开(公告)号：EP3174997B1
公开(公告)日：2018-12-05
申请号：EP15744927.3
申请日：2015-07-31
申请人： Olink Bioscience AB
发明人： LANDEGREN, Ulf , NONG, Yuan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6827 , C12Q2525/131 , C12Q2525/161 , C12Q2525/301 , C12Q2525/307 , C12Q2531/125 , C12Q2533/107 , C12Q2537/149 , C12Q2537/159
摘要： The present invention relates to a method of selecting a target region of interest (ROI) in a target nucleic acid molecule using a nucleic acid probe comprising sequences capable of directing the cleavage of a target nucleic acid molecule to release a fragment comprising the ROI and sequences capable of templating the circularisation and ligation of the target fragment. The circularised molecule thus obtained contains the selected ROI and may be subjected to further analysis and/or amplification etc. Also provided are probes and kits for use in such methods.

5. 发明公开

EP3174997A1 METHOD FOR SELECTING A TARGET NUCLEIC ACID SEQUENCE 有权
标题翻译：用于选择目标核酸序列的方法
公开(公告)号：EP3174997A1
公开(公告)日：2017-06-07
申请号：EP15744927.3
申请日：2015-07-31
申请人： Olink Bioscience AB
发明人： LANDEGREN, Ulf , NONG, Yuan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6827 , C12Q2525/131 , C12Q2525/161 , C12Q2525/301 , C12Q2525/307 , C12Q2531/125 , C12Q2533/107 , C12Q2537/149 , C12Q2537/159
摘要： The present invention relates to a method of selecting a target region of interest (ROI) in a target nucleic acid molecule using a nucleic acid probe comprising sequences capable of directing the cleavage of a target nucleic acid molecule to release a fragment comprising the ROI and sequences capable of templating the circularisation and ligation of the target fragment. The circularised molecule thus obtained contains the selected ROI and may be subjected to further analysis and/or amplification etc. Also provided are probes and kits for use in such methods.
摘要翻译：本发明涉及使用核酸探针选择靶核酸分子中的目标靶区（ROI）的方法，所述核酸探针包含能够指导靶核酸分子裂解的序列以释放包含ROI的片段和序列能够模板化目标片段的环化和连接。如此获得的环化分子包含选定的ROI并且可以进行进一步的分析和/或扩增等。还提供了用于这种方法的探针和试剂盒。

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